Schizophyllum commune induced oxidative stress and immunosuppressive activity in Spodoptera litura

Abstract Background: In a search for the mechanism of action of an endophytic fungus Schizophyllum commune against Spodoptera litura, the effect of its ethyl acetate extract was judged on antioxidant and detoxifying enzymes as well as on morphology of haemocytes. Results: Ethyl acetate extract of S.commune was administrated to the larvae of S.litura using artificial diet method having concentration 276.54µg/ml (LC50 of fungus). The effect on antioxidant and detoxifying enzymes (Catalase, Ascorbate peroxidase, Superoxide dismutase, Glutathione-S-Transferase) was observed using haemolymph and midgut of insect larvae for different time intervals (24, 48, 72 and 96). In additionally, haemocytes morphology was also studied using Scanning Electron Microscopy (SEM) after 96hr. In particular, to resist the toxicity, the activities of the antioxidant and detoxifying enzymes (Catalase, Ascorbate peroxidase, Superoxide dismutase, Glutathione-S-Transferase) significantly (p≤0.05) increased in both the tissues of treated larvae as compared to control. The exposed group revealed various deformities in haemocytes of S.litura like breakage in the cell membrane, cytoplasmic leakage and appearance of strumae etc. Conclusion: This study provides important information regarding the oxidative stress causing potential and immunosuppressant nature of S.commune against S.litura and elucidates the patterns of antioxidant and detoxifying enzymes activities and changes in haemocytes in the susceptible larvae.

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Schizophyllum commune induced oxidative stress and immunosuppressive activity in Spodoptera litura

10.21203/rs.2.21186/v2 ◽

2020 ◽

Author(s):

Mandeep Kaur ◽

Pooja Chadha ◽

Sanehdeep Kaur ◽

Amarjeet Kaur ◽

Rajvir Kaur

Keyword(s):

Oxidative Stress ◽

Spodoptera Litura ◽

Artificial Diet ◽

Ethyl Acetate Extract ◽

Fungal Endophytes ◽

Schizophyllum Commune ◽

Immunosuppressive Activity ◽

Glutathione S Transferase ◽

Drastic Reduction ◽

Detoxifying Enzymes

Abstract Background: In the last few decades, considerable attention has been paid to fungal endophytes as biocontrol agents, however little is known about their mode of action. This study aimed to investigate the toxic effects of an endophytic fungus Schizophyllum commune by analyzing activities of antioxidant and detoxifying enzymes as well as morphology of haemocytes using Spodoptera litura as a model. Results: Ethyl acetate extract of S.commune was fed to the larvae of S.litura using the artificial diet having 276.54µg/ml (LC 50 of fungus) concentration for different time durations. Exposed groups revealed, significant (p≤0.05) increase in the activities of various enzymes viz. Catalase, Ascorbate peroxidase, Superoxide dismutase, Glutathione-S-Transferase. Furthermore, haemocytes showed various deformities like breakage in the cell membrane, cytoplasmic leakage and appearance of strumae in the treated larvae. A drastic reduction in the percentage of normal haemocytes was recorded in the treated groups with respect to control. Conclusion: The study provides important information regarding the oxidative stress causing and immunosuppressant potential of S.commune against S.litura and its considerable potential for incorporation in pest management programmes.

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Aspergillus Flavus Induced Oxidative Stress and Immunosuppressive Activity in Spodoptera Litura As Well As Safety for Mammals

10.21203/rs.3.rs-32331/v1 ◽

2020 ◽

Author(s):

Mandeep Kaur ◽

Pooja Chadha ◽

Sanehdeep Kaur ◽

Amarjeet Kaur

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Ethyl Acetate ◽

Spodoptera Litura ◽

Ethyl Acetate Extract ◽

Immune Defense ◽

Malondialdehyde Content ◽

Liver And Kidney ◽

Antioxidant Enzymes Activities ◽

Cellular Immune

Abstract In an attempt to find toxicity of Aspergillus flavus in insects, Spodoptera litura was treated with A.flavus ethyl acetate extract and toxicity was assessed by analyzing the effect of fungal extract on antioxidant and cellular immune defense. In antioxidant defense, the lipid peroxidation (Malondialdehyde content) and antioxidant enzymes activities (Catalase, Ascorbate peroxidase, Superoxide dismutase) were examined. In cellular immune defense, effect of A. flavus extract was analyzed on haemocytes using Scanning Electron Microscopy (SEM). Ethyl acetate extract of A. flavus was administrated to the larvae of S.litura using artificial diet method having concentration 1340.84µg/ml (LC50 of fungus). The effect was observed using haemolymph of insect larvae for different time intervals (24, 48, 72 and 96). In particular, Malondialdehyde content and antioxidant enzymes activities were found to be significantly (p≤0.05) increased in treated larvae as compared to control. A. flavus ethyl acetate extract also exhibit negative impact on haemocytes having major role in cellular immune defense. Various deformities were observed in different haemocytes like cytoplasmic leakage and surface abnormalities etc. Furthermore, mammalian toxicity was analyzed with respect to DNA damage induced in treated rat relative to control. Genotoxicity on rat was assessed using different tissues of rat (blood, liver, and kidney) by comet assay. Non-significant effect of A. flavus extract was found in all the tissues (blood, liver, and kidney). Overall the study provides important information regarding the oxidative stress causing potential and immunosuppressant nature of A. flavus against S. litura and its non toxicity to mammals (rat).

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Ameliorative Effects of Indole-3-Carbinol and/or Sulforaphane Against Gold Nanoparticle-Induced Cytotoxicity in Rats

Science of Advanced Materials ◽

10.1166/sam.2021.3891 ◽

2021 ◽

Vol 13 (2) ◽

pp. 222-234

Author(s):

Maha I. Alkhalaf ◽

Wafa S. Alansari

Keyword(s):

Oxidative Stress ◽

Gold Nanoparticles ◽

Superoxide Dismutase ◽

Creatine Kinase ◽

Albino Rats ◽

Functional Markers ◽

Related Factor ◽

Nuclear Factor ◽

Glutathione S Transferase ◽

Total Antioxidant

Gold nanoparticles (GNPs) are the most commonly used metal nanoparticles due to their promising characteristics. However, application of GNs in medical and biological fields has resulted in toxicity to several organs. Indole-3-carbinol (I3C) and sulforaphane (SF) are the two well-known natural compounds, largely present in cruciferous vegetables. This study aimed to explore the therapeutic efficacy of I3C and SF alone or in combination against GN-induced renal and cardiac toxicities. Fifty male Albino rats were randomly segregated into five groups with each group containing 10 rats; G1, control; G2, intraperitoneally administered with a suspension of GNPs (10 nm in size; 20 µg/kg body weight (b.w.) for 7 days; G3, GN-injected rats, supplemented with SF (5 mg/kg b.w) daily for 7 days; G4, GN-injected rats, supplemented orally with I3C (150 mg/kg b.w.) for 7 days and G5, GN-injected rats supplemented orally with SF and I3C daily for 7 days. GN treatment significantly disturbed kidney functional markers, as evidenced by significantly increased levels of urea, creatinine and creatine kinase. Additionally, GNs significantly increased renal and cardiac levels of malondialdehyde, 8-hydroxydeoxyguanosine and interleukin-6, and depleted, glutathione S-transferase, glutathione reductase, superoxide dismutase, total antioxidant capacity, and nuclear factor erythroid 2-related factor 2. In contrast, treatment with I3C and SF alone or in combination significantly restored all the parameters to their near normal levels. GN induced histological abnormalities were also significantly attenuated. Taken together, the data indicate that the SF and I3C are more effective when given separately than when given together in lowering GN-induced toxicity by their ability to alleviate oxidative stress and inflammation.

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Precompetitional Weight Reduction Modifies Prooxidative-Antioxidative Status in Judokas

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/2164698 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Katarzyna Knapik ◽

Karolina Sieroń ◽

Ewa Wojtyna ◽

Grzegorz Onik ◽

Ewa Romuk ◽

...

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Weight Reduction ◽

Glutathione S Transferase ◽

Second Stage ◽

The Third ◽

Third Stage ◽

Protein Sulfhydryl ◽

Total Oxidative Stress

Objective. The main aim of the study was an assessment of the influence of rapid weight loss on oxidative stress parameters in judokas differing in weight reduction value. Materials and Methods. The study included 30 judokas with an age range of 18-30 years (mean age: 22.4±3.40 years). Enzymatic and nonenzymatic antioxidative markers, lipid peroxidation markers, and total oxidative stress were assessed three times: one week before a competition (the first stage), after gaining the desired weight (the second stage), and one week after the competition (the third stage). Results. Between the first and the second stage, the concentration of lipid hydroperoxides (LPH) decreased significantly. The superoxide dismutase (SOD), copper- and zinc-containing superoxide dismutase (Cu,Zn-SOD), ceruloplasmin (CER), malondialdehyde (MDA), LPH, and total oxidative stress (TOS) concentrations were the lowest one week after the competition. Linear regression indicated that the emphases on increased weight reduction increased the activity of glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), and protein sulfhydryl (PSH) between the first and the second stage of the study. Moderate weight reduction (2-5%) resulted in elevated levels of SOD, Mn-SOD, LPH, MDA, and TOS in comparison to low and high reductions. An opposite relation was observed in PSH. In judokas, the precompetitional weight reduction range was 0.44-6.10% (mean: 2.93%±1.76%) of the initial body weight. Concentrations of superoxide dismutase (SOD; p<.01), manganese-dependent superoxide dismutase (Mn-SOD; p<.001), and ceruloplasmin (CER; p<.05) decreased between the first and the third stage of the study as well between the second and third one. Before competitions, a decrease in lipid hydroperoxide (LPH; p<.01) concentration was observed. A reduction of malondialdehyde (MDA; p<.05), LPH (p<.01), and total oxidative stress (TOS; p<.05) levels between the first and the final stage occurred. The increase in weight reduction was linearly correlated with the rise of glutathione peroxidase (GPx; p<.05), glutathione reductase (GR; p<.05), glutathione S-transferase (GST; p<.05), and protein sulfhydryl (PSH; p<.05) concentrations between the first and the second stage of the study. Moderate weight reduction (2-5%) resulted in elevated levels of SOD (p<.05), Mn-SOD (p<.05), LPH (p<.05), MDA (p<.05), and TOS (p<.05) in comparison to low and high reductions. An opposite relation was observed in PSH (p<.005). Conclusions. The effect of weight reduction in judo athletes on prooxidative-antioxidative system diversity depends on the weight reduction value.

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