scholarly journals Phytochemical screening and anti-oxidant activity of Sargassum wightii enhances the anti-bacterial activity against Pseudomonas aeruginosa

Author(s):  
Govindan Nadar Rajivgandhi ◽  
Chelliah Chenthis Kanisha ◽  
Govindan Ramachandran ◽  
Natesan Manoharan ◽  
Ramzi A. Mothana ◽  
...  
2019 ◽  
Vol 1 (2) ◽  
pp. 1-12
Author(s):  
Jamilah Abbas

Members of Calophyllum (Calophyllaceae) are commonly used as herbal medicine in Indonesia from the beginning of civilization. Due to their tremendous potential as a source for pharmaceutical industry, we selected 11 Calophyllum species for the current anti-oxidant and anti-bacterial studies, namely, Calophyllum euryphyllum, C. bicolor, C. flavoramulum, C incrassaptum, C. lowei, C. macrophyllum, C. nodosum, C. soulattri, C. tetrapterum, C. teysmannii, and C. tomentosum. The methods include Calophyllum stem bark extraction and separation into n-hexane, ethyl acetate, and butanol fractions, followed by the antioxidant analysis using DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging) and the anti-bacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa. The results showed that n-hexane fraction of C. soulattri exhibited the best anti-oxidant activity (IC50 = 2.2 µg/m). This study also showed that a xanthone compound isolated from C. macrophyllum, namely, 1,8 - dihydroxy-6 methoxy xanthone exhibited the anti-oxidant activity with IC50 = 1.56 ppm.


2014 ◽  
Vol 21 ◽  
pp. 10-21
Author(s):  
M. Geetha Eswari ◽  
R. Lakshmi Rathi ◽  
J. Harini ◽  
R. Aruna ◽  
V. Balakrishnan

The plant Justicia simplex D.Don is traditional herb, people used it in different diseases, they treat fever and burning of the body, strengthen the lungs, the teeth and stop vomiting. The plant exhibited more promising bacterial activity against various microorganisms. Hence, in the present study this plant is also tested against the dental pathogens. The plant was extracted from four different solvents was tested for the presence of phytochemicals in it. After analyzing, its antimicrobial activity is tested against fungi and bacteria. Then a dental carrier Streptococcus aureus, Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa which was collected from the K.S.R. Institute for Dental Science and Research. The isolates were used to check the activity against Justicia simplex D.Don. Based on the positive result obtained against various analyses, it is formulated successfully into a tooth paste. The present study on Phytochemical screening of Justicia simplex D.Don a valuable medicinal plant extract against Dental pathogens enables the use of this plant for the formulation of tooth powder against dental pathogens. The result is more effective against dental pathogens by herbal tooth powder


Author(s):  
Hamza Mohamed Ahmed ◽  
Ashraf Mahmoud Ramadhani ◽  
Ibrahim Yaagoub Erwa ◽  
Omer Adam Omer Ishag ◽  
Mohamed Bosharh Saeed

cinnamon dating from 1000 AD when it was firstly recorded in English due to its important as aroma and as herbs. The aim of this study was to investigate phytochemicals constitutes, chemical composition and antimicrobial activity of the essential oil of commercial samples of Cinnamon verum bark. The essential oil was extracted by hydrodistillation, while the crude extracts were prepared by three different solvents methanol (70%), acetone and aqueous. Phytochemical screening of crude extracts was performed using standard methods. The essential oil was subjected to GC-MS analysis and tested against Staphylococcus aureus, Salmonella typhimurium, Pseudomonas aeruginosa, Eschericchia coli and Candida albicans. The obtained results indicated the presence of alkaloids, flavonoids, coumarin, tannins, terpenoids, saponin, glycoside, anthrocyanin and phenolic compounds in the methanolic, aqoueous and acetone extracts of C. verum bark; while the major components of the extracted essential oil of C. verum bark were cinnamaldehyde (85.50%), stigmasterol (3.69%), Cadinene (1.37%), (E)-cinnamaldehyde (1.35%), alpha-amorphene (1.33%), hydrocinnamaldehyde (1.28%), alpha-cubebene (1.25) and ergosterol (1.09%) respectively. The antimicrobial activity result indicated the high activity of the extracted essential oil against all tested microorganisms at high concentration; except in S. typhimurium and C. albicans at concentrations of 25% and 12.5% no activity was noticed. Based in our obtained results the essential oil of C. verum bark had high potential as antimicrobial agent, therefore, recommended for more advanced studies to be conducted on this abundant plant as natural source of antibiotics.


Author(s):  
Moses Owolabi

Introduction: Medicinal plants play a significant role in the health maintenance in underdeveloped countries, herbs and spices continue serve as a new sources for herbal medicines. Aim: The objective was to investigate phytochemical screening and biocidal activity on stem bark Chrysophyllum albidum (Linn) and straw Aristolochia ringens using chloroform. Methods: Chloroform extracts of C. albidum and A. ringens were obtained and screened for phytochemical constituents. The colour intensity or the precipitate formation was used as analytical responses to these tests. The Antimicrobial activities crude extracts were evaluated against some microorganisms with broth microdilution test. The extracts were screened for in-vitro cytotoxic activity against breast tumour and liver tumour cells. Results: The results of the phytochemical screening revealed the presence of alkaloids, saponins, steroids, anthraquinone and glycosides. C. albidum and A. ringens chloroform extracts revealed high quantities of alkaloid and flavonoid respectively. While cardiac glycoside and anthraquinone were found with low quantity in C. albidum and A. ringens extracts respectively. C. albidum showed promising bacterial activity against Escherichia coli and cytotoxic activity to liver tumour cells. While A. ringens, showed excellent bacterial activity against Bacillus cereus and good fungal activity against Candida albicans. A. ringens also showed cytotoxic to liver tumour and breast tumour cells. The observed antimicrobial and cytotoxic effects may be due to the antagonist or synergistic effect of the secondary metabolites identified in the extracts. Conclusion: Medicinal plants studied showed good potential biological activities that support the idea that traditional medicines remain useful healthcare in developing countries


2011 ◽  
Vol 25 (3-4) ◽  
pp. 187-195 ◽  
Author(s):  
M. Nasiruzzaman Shaikh ◽  
Bassem A. Al-Maythalony ◽  
Mohammed I. M. Wazeer ◽  
Anvarhusein A. Isab

Cadmium and mercury selenocyanate complexes of 2-thiouracil (TU) and 2,4-dithiouracil (DTU) ligands have been synthesized to form complexes of the type [M(SeCN)2(TU)] and [M(SeCN)2(DTU)] (where M is Cd2+or Hg2+) and studied by various spectroscopic techniques such as IR,1H and13C NMR in solution and in the solid state for13C,15N, and113Cd nuclei. Based on IR, and solution and solid-state13C NMR data, stronger cadmium bonding to the thiouracil was observed compared to that of mercury. Anti-bacterial activities of these complexes have been investigated with standard type culture ofEscherichia coli(MTCC 443),Klebsiella pneumoniae(MTCC 109),Pseudomonas aeruginosa(MTCC 1688),Salmonella typhi(MTCC 733) andStaphylococcus aureus(MTCC 737) and show that ligands exhibit more anti-bacterial activities than that of the corresponding Cd(II) and Hg(II) complexes.


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