Cross-sectional study on seroprevalence and risk factor analysis of Mycobacterium avium subsp. paratuberculosis in Kashmir Merino sheep flocks of Central Kashmir valley, India

2020 ◽  
Vol 193 ◽  
pp. 106266
Author(s):  
Adil Majid Bhat ◽  
Hamidullah Malik ◽  
Masood Saleem Mir ◽  
Kundan Kumar Chaubey ◽  
S.V. Singh
Keyword(s):  
Factor Analysis ◽  
Risk Factor ◽  
Mycobacterium Avium ◽  
Cross Sectional Study ◽  
Mycobacterium Avium Subsp ◽  
Sectional Study ◽  
Kashmir Valley ◽  
Cross Sectional ◽  
Merino Sheep ◽  
Mycobacterium Avium Subsp Paratuberculosis

scholarly journals Mycobacterium avium subsp. paratuberculosis antibodies in cows of low-tropic dairy herds in Colombia

2020 ◽  
pp. e1782
Author(s):  
Nathalia María del Pilar Correa-Valencia ◽  
Ferney Arango-Lezcano ◽  
Jorge Arturo Fernández-Silva
Keyword(s):  
Mycobacterium Avium ◽  
Positive Outcome ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Mycobacterium Avium Subsp ◽  
Dairy Herds ◽  
Cross Sectional ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Level Variable ◽  
Associated Variables

Objective. To report the frequency of seropositivity against Mycobacterium avium subsp. paratuberculosis (MAP) and to explore the factors associated with the positive outcome, both at herd and animal level. Materials and methods. A cross-sectional study was carried out on 204 dairy cows, result of the crossing of different breeds, from five low-tropic dairy herds, located in three municipalities of the Provinces of Sucre and Córdoba (Colombia) in 2018. The animals were randomly selected, and blood samples were collected from each one. A commercial ELISA kit was used to analyze the sera, and the association between these variables and the result to ELISA (p<0.05) was explored. Results. The 17.2% (35/204; 95% CI: 12.0-22.3%) of the cows were positive for MAP by the ELISA test, and the five herds had seropositive animals. The herd-level variable presence of other ruminants in co-grazing with cattle in the last 2 years and the animal-level variables age and parity were associated with the positive ELISA results. Conclusions. The present study found that 17.2% of the cows and 100% of the herds were MAP positive by the ELISA test. Additionally, associated variables were identified that may be of interest to both producers and veterinarians and guide their approach to disease management.

scholarly journals Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in Brazilian patients with inflammatory bowel diseases and in controls

2015 ◽  
Vol 134 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Isabel Azevedo Carvalho ◽  
David Germano Gonçalves Schwarz ◽  
Pricila Aparecida Grasse Pietralonga ◽  
Ana Carolina Silva Faria ◽  
Isis Freitas Espechit Braga ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Bacterial Load ◽  
Cross Sectional Study ◽  
Mycobacterium Avium Subsp ◽  
Control Group ◽  
Cross Sectional ◽  
Qrt Pcr ◽  
Ffpe Samples ◽  
Inflammatory Bowel ◽  
Mycobacterium Avium Subsp Paratuberculosis

CONTEXT AND OBJECTIVE: Mycobacterium avium subsp. paratuberculosis (MAP) has attracted the interest of researchers because of similarities between paratuberculosis and Crohn's disease (CD). The aim of this study was to evaluate the frequency of MAP through cultures, histology and polymerase chain reaction (PCR) on intestinal biopsies from Brazilian CD patients. Quantitative real time PCR (qRT-PCR) was performed on positive samples. DESIGN AND SETTING: Analytical cross-sectional study with control group at two federal universities. METHODS: Fresh samples were collected from 25 patients; five with CD, eight with ulcerative colitis (UC) and 12 controls with non-inflammatory bowel disease (nIBD). Formalin-fixed paraffin-embedded (FFPE) samples from 143 patients were also collected: 44 CD, 49 UC and 56 nIBD. RESULTS: None of the fresh samples was positive for MAP. Five FFPE samples (one CD, two UC and two nIBD) and three fresh samples (one in each group) were positive through IS900-PCR. qRT-PCR was performed on these eight samples. Among the FFPE samples, there were 192.12 copies/μl in the CD group, 72.28 copies/μl in UC and 81.43 copies/μl in nIBD. Among the fresh samples, there were 432.99 copies/μl, 167.92 copies/μl and 249.73 copies/μl in the CD, UC and nIBD groups, respectively. The highest bacterial load was in the CD group. CONCLUSION: This study does not provide evidence for a role of MAP in the etiology of CD, although MAP DNA was detected in all three patient groups. This is the first report of MAP presence in human intestinal biopsies in Brazil.

Risk factor analysis for antibodies to Brucella, Leptospira and C. burnetii among cattle in the Adamawa Region of Cameroon: a cross-sectional study

2012 ◽  
Vol 45 (2) ◽  
pp. 617-623 ◽  
Author(s):  
Stella Mazeri ◽  
Francesca Scolamacchia ◽  
Ian G. Handel ◽  
Kenton L. Morgan ◽  
Vincent N. Tanya ◽  
...  
Keyword(s):  
Factor Analysis ◽  
Risk Factor ◽  
Cross Sectional Study ◽  
Risk Factor Analysis ◽  
Sectional Study ◽  
Cross Sectional

Eyelid Dermatitis and Contact Sensitization to Nickel: Results from an Italian Multi-Centric Observational Study

2019 ◽  
Vol 19 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Alessandro Borghi ◽  
Monica Corazza ◽  
Elisa Maietti ◽  
Cataldo Patruno ◽  
Maddalena Napolitano ◽  
...  
Keyword(s):  
Risk Factor ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Contact Sensitization ◽  
Main Risk Factor ◽  
Nickel Allergy ◽  
Cross Sectional ◽  
Triggering Factor ◽  
Irritant Action ◽  
Allergic Contact

Background: Due to the sensitizing constituents of eye cosmetics, allergic contact dermatitis is considered a frequent cause of eyelid dermatitis. An association between eyelid dermatitis and nickel contained in make-ups remains controversial. Objective: The study aimed to assess the association between nickel allergy, the use of pigmented makeup products and self-reported eyelid dermatitis. Method: This multi-centric, cross-sectional study enrolled 165 women sensitized to nickel (patients) and 103 women without intolerance to metals (controls). We recorded: demographics, atopy, use of pigmented eye cosmetics (mascara, eyeshadow, eyeliner, eyebrow pencil), and previous eyelid dermatitis. Among the patients, any co-sensitization to cosmetics or metals was recorded. Results: 87.3% of the patients and 91.3% of the controls reported their use of eye make-up; 44.9% and 52.4%, respectively, reported previous episodes of eyelid dermatitis, without significant differences. The occurrence of eyelid dermatitis was significantly associated with the use of eye make-up products, both in general and considering each product separately. Age, atopy, or co-sensitization to other metals or cosmetics did not affect the occurrence of eyelid dermatitis. Conclusion: Nickel allergy should not be considered the main risk factor for eyelid dermatitis. The use of pigmented eye make-up may be a triggering factor for eyelid dermatitis, probably due to an irritant action.

scholarly journals Development and validation of a Japanese version of the Patient Centred Assessment Method and its user guide: a cross-sectional study

BMJ Open ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. e037282
Author(s):  
Rieko Mutai ◽  
Yoshifumi Sugiyama ◽  
Shuhei Yoshida ◽  
Ryoko Horiguchi ◽  
Takamasa Watanabe ◽  
...  
Keyword(s):  
Primary Care ◽  
Factor Analysis ◽  
Japanese Version ◽  
Well Being ◽  
Cross Sectional Study ◽  
Assessment Method ◽  
Sectional Study ◽  
Cross Sectional ◽  
Vas Scores ◽  
Patient Complexity

ObjectivesThe primary objective of this study was to develop the Japanese version of the Patient Centred Assessment Method (PCAM) and its user guide. The secondary objective was to examine the validity and reliability in the primary care setting.DesignCross-sectional study.SettingThree family physician teaching clinics located in urban residential areas in Tokyo, Japan.ParticipantsPatients who were aged 20 years or older, and who had an appointment with physicians at the three participating clinics.Main outcome measuresPatient complexity measured by PCAM and complexity/burden level measured by a Visual Analogue Scale (VAS).ResultsAlthough confirmatory factor analysis using a model described in a previous study revealed that the indices did not meet the criteria for good fit, exploratory factor analysis revealed a new three-factor structure of ‘Personal well-being,’ ‘Social interaction’ and ‘Needs for care/service.’ Cronbach’s alpha of PCAM was 0.86. Spearman’s rank correlation coefficients between PCAM scores and VAS scores were 0.51 for complexity (p<0.001) and 0.41 for burden (p<0.001). There were 42 patients (14.3% of total patients) with PCAM scores greater than its mean of 16.5 but with complexity VAS scores less than its mean of 20.8.ConclusionsThe Japanese version of PCAM and its user guide were developed through Japanese translation and cultural adaptation by cognitive debriefing. PCAM is a valid and reliable tool to assess patient complexity in the primary care settings in Japan. Additionally, although the correlation between total PCAM scores and complexity/burden as assessed by VAS was moderate, PCAM can more precisely identify patient complexity than skilled physician’s intuition.

scholarly journals Correction to: Psoriasis as risk factor for non-ischemic dilated cardiomyopathy: a population-based cross-sectional study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Abbas Alshami ◽  
Nasam Alfraji ◽  
Steven Douedi ◽  
Swapnil Patel ◽  
Mohammad Hossain ◽  
...  
Keyword(s):  
Risk Factor ◽  
Dilated Cardiomyopathy ◽  
Population Based ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Non Ischemic Dilated Cardiomyopathy

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