The age-related changes of Ca2+ channel currents were investigated in freshly isolated single smooth muscle cells from the circular layer of the distal colon from the rat using the whole cell voltage clamp technique. Under physiological conditions (Ca2+ concentration of 2.0 mM), the averaged total Ca2+ current density increased markedly from 1.25 pA/pF in the newborn rat to 6.46 pA/pF in the 60-day-old rat; it then gradually declined with aging. Two types of Ca2+ channel currents seemed to be present; one type possessed more negative threshold potentials (-70 to -60 mV) when the cells were held at -80 or -100 mV and inactivated quickly. The voltage for peak current was -20 to -10 mV, and the reversal potential was +60 to +70 mV. This current was highly sensitive to low concentrations of Ni2+ (30 microM) but was resistant to nifedipine, diltiazem, cadmium, and tetrodotoxin. In contrast, the other type of Ca2+ channel current possessed more positive threshold potential (-40 mV) and inactivated more slowly. The voltage for peak current was 0 mV, and the reversal potential was +60 to +70 mV. This current was insensitive to low concentrations of Ni2+ but highly sensitive to nifedipine, diltiazem, and cadmium. These results suggest that the fast inactivating (transient) current might be T-type Ca2+ current [ICa(T)], and such cells were ICa(T) positive cells; whereas the sustained Ca2+ current was L-type Ca2+ current [ICa(L)], and such cells were ICa(L) positive cells. Our results showed that the fraction of ICa(T) positive cells increased with development; the current densities of both ICa(L) and ICa(T) also increased with development.(ABSTRACT TRUNCATED AT 250 WORDS)
Short Barriers for Lowering Current-Density in Terahertz Quantum Cascade Lasers
