Allicin ameliorates intraintestinal bacterial translocation after trauma/hemorrhagic shock in rats: The role of mesenteric lymph node dendritic cell

Surgery ◽  
2017 ◽  
Vol 161 (2) ◽  
pp. 546-555 ◽  
Author(s):  
Yun Zhang ◽  
Jian Zhang ◽  
Tao Xu ◽  
Wei Wu ◽  
Fang-Fang Huang ◽  
...  
Keyword(s):  
Lymph Node ◽  
Dendritic Cell ◽  
Hemorrhagic Shock ◽  
Bacterial Translocation ◽  
Mesenteric Lymph Node ◽  
Mesenteric Lymph

Bacteremia and Bacterial Translocation in the Naturally Occurring Canine Gastric Dilatation-Volvulus Patient

2003 ◽  
Vol 39 (4) ◽  
pp. 361-368 ◽  
Author(s):  
Kevin P. Winkler ◽  
Cathy L. Greenfield ◽  
David J. Schaeffer
Keyword(s):  
Lymph Node ◽  
Prospective Study ◽  
Bacterial Translocation ◽  
Mesenteric Lymph Node ◽  
Blood Cultures ◽  
Gastric Dilatation ◽  
Gram Negative ◽  
Bacterial Cultures ◽  
Naturally Occurring ◽  
Mesenteric Lymph

This prospective study was performed to determine the prevalence of bacteremia in the naturally occurring gastric dilatation-volvulus (GDV) patient, the possible relationship between bacteremia and survival, and whether bacteremia was a result of translocation from the stomach. Blood cultures were collected from each patient. Bacterial cultures were collected from the liver, mesenteric lymph node, and stomach. Forty-three percent of the GDV cases and 40% of the controls developed positive blood cultures. Gram-negative rods were the most frequently isolated organisms. Evidence of bacterial translocation from the stomach could not be demonstrated in GDV patients, and survival was not affected by the presence of bacteremia.

Detection of bacterial translocation in mesenteric lymph node by in situ PCR in rats with acute pancreatitis.

1997 ◽  
Vol 8 (8) ◽  
pp. 353-354
Author(s):  
Keiichi Ikegami ◽  
Tetsuo Sakai ◽  
Yasushi Nagashima ◽  
Akira Wakizaka ◽  
Eiichi Matsuo ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Lymph Node ◽  
Bacterial Translocation ◽  
Mesenteric Lymph Node ◽  
In Situ Pcr ◽  
Mesenteric Lymph

scholarly journals CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis

2012 ◽  
Vol 42 (12) ◽  
pp. 3150-3166 ◽  
Author(s):  
Samira Tamoutounour ◽  
Sandrine Henri ◽  
Hugues Lelouard ◽  
Béatrice de Bovis ◽  
Colin de Haar ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Mesenteric Lymph

CD4 T cells activated in the mesenteric lymph node mediate gastrointestinal food allergy in mice

2007 ◽  
Vol 293 (6) ◽  
pp. G1234-G1243 ◽  
Author(s):  
Adina Kay Knight ◽  
Ana Belén Blázquez ◽  
Shu Zhang ◽  
Lloyd Mayer ◽  
Hugh A. Sampson ◽  
...  
Keyword(s):  
T Cells ◽  
Food Allergy ◽  
Lymph Node ◽  
T Cell ◽  
T Cell Activation ◽  
Mesenteric Lymph Node ◽  
Cell Activation ◽  
Food Challenge ◽  
Mesenteric Lymph

A localized Th2 milieu has been observed in the intestine of subjects with food allergic disorders; however, the role of T cells in the pathophysiology of these disorders remains poorly understood. Our aim was to examine sites of T cell activation in response to food challenge, identify potential factors responsible for T cell recruitment to the gut, and determine the role of T cells in disease. BALB/c mice were systemically sensitized to ovalbumin (OVA) and repeatedly fed with OVA to induce allergic diarrhea. Local cytokine and chemokine expressions were assessed by quantitative PCR, and cytokine secretion levels in the mesenteric lymph node (MLN) were determined by ELISA. Homing molecule expression was determined by flow cytometry, and the role of CD4+ T cells in promoting disease was tested by adoptive transfer. Mice developed diarrhea associated with changes in epithelial ion transport, mast cell infiltration, intestinal IgE secretion, and local upregulation of Th2 cytokines and the Th2 chemokines CCL1, CCL17, and CCL22 in the small intestine. T cell activation occurred in the MLN before symptom onset, and a single feed of OVA induced T cell proliferation, α4β7 upregulation, and CD62L downregulation. Cells from the MLN, including purified CD4+ T cells, were able to transfer allergic diarrhea to naive mice. A gut-homing phenotype induced in the MLN and selective upregulation of Th2 chemoattractants are likely important factors in the gastrointestinal recruitment of pathological Th2-skewed CD4+ T cells in food allergy.

scholarly journals The Effect of Curcumin on an Animal Intestinal Ischemia/Reperfusion Model for Bacterial Translocation and Inflammatory Response

2015 ◽  
Vol 100 (11-12) ◽  
pp. 1352-1359
Author(s):  
Selim Sözen ◽  
Mehmet Aziret ◽  
İlhan Bali ◽  
Seyfi Emir ◽  
Yiğit Ülgen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Inflammatory Response ◽  
Bacterial Translocation ◽  
Mesenteric Lymph Node ◽  
Ischemia Reperfusion ◽  
Chain Reaction ◽  
Blood Samples ◽  
Mesenteric Lymph ◽  
Polymerase Chain

Ischemia/reperfusion (IR) injury of the intestine is a major problem in abdominal pathological condition and is associated with a high morbidity and mortality. The purpose of the study is to investigate the effects of curcumin on the bacterial translocation incidence and inflammatory response in rats submitted to bowel ischemia reperfusion injury. Thirty-two Wistar albino rats with a weight of 200 to 250 g were used in the study. They were randomly divided into 3 groups (n = 10 for each group): sham only operated group(group I); IR group (group II); and IR + curcumin treatment group (group III). Curcumin (curcumin from Curcuma longa) 20 mg/kg/day was given orally to the curcumin group. All animals were given 109 E. Coli by orogastric intubation 12 hours before sampling. Seventy-two hours after the first operation, mesenteric lymph node and blood samples were obtained and cultured. Blood samples of 2 mL were obtained for a polymerase chain reaction study. A piece of terminal ileum was also sampled for histopathologic examination. Mesenteric lymph node and blood cultures of all control animals were positive for microbiological growth, and polymerase chain reaction results were positive in seven of the eight rats. Histopathologically, edema, vasodilatation and inflammatory cell infiltration were found to be less in the other groups in comparison to the control group. Curcumin reduced bacterial translocation in blood, hepatocellular damage, and plasma cytokine levels. Curcumin reduced the incidence of bacterial translocation in intestinal I/R. rats. These results suggest that Curcumin would be clinically useful in the treatment of intestinal I/R injury.

Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node

2003 ◽  
Vol 284 (6) ◽  
pp. H2213-H2226 ◽  
Author(s):  
Mitchell H. Grayson ◽  
Richard S. Hotchkiss ◽  
Irene E. Karl ◽  
Michael J. Holtzman ◽  
David D. Chaplin
Keyword(s):  
Lymph Node ◽  
T Cell ◽  
T Lymphocytes ◽  
Mesenteric Lymph Node ◽  
White Pulp ◽  
Cell Trafficking ◽  
High Endothelial Venules ◽  
Rolling Velocity ◽  
Mesenteric Lymph

Lymphocyte rolling velocity is determined largely by interactions between leukocyte α4-integrin (CD49d) and L-selectin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mesenteric postcapillary venules and Peyer's patch high endothelial venules (HEVs). The role of these interactions in other tissue sites of lymphocyte emigration is not known. With the use of real-time intravital confocal microscopy, we found that rolling velocities of T lymphocytes in the murine mesenteric lymph node (MLN) HEV also depend on L-selectin and CD49d. However, in the murine spleen, rolling velocities of T lymphocytes are not influenced by the loss of L-selectin and CD49d. With the use of FITC-dextran and TIE2-GFP mice, we further defined the microvascular compartments of the spleen and showed that adherence of T cells is localized to regions in the white pulp that are not lined by endothelial cells and have shear rates similar to bone marrow sinusoids. These results establish that T cell trafficking to the spleen differs from trafficking to other secondary lymphoid organs and suggest that the mechanical properties of the blood-filtering role of the spleen are important in T cell accumulation in the organ.

Endogenous infection in mice with streptozotocin-induced diabetes. A feature of bacterial translocation

1984 ◽  
Vol 30 (11) ◽  
pp. 1344-1348 ◽  
Author(s):  
Akihiro Imai ◽  
Yoko Kurihara
Keyword(s):  
Lymph Node ◽  
Bacterial Translocation ◽  
Mesenteric Lymph Node ◽  
Staphylococcus Epidermidis ◽  
Anaerobic Bacteria ◽  
Bacterial Strains ◽  
Indigenous Bacteria ◽  
Mesenteric Lymph ◽  
Single Intraperitoneal Injection ◽  
Streptozotocin Induced Diabetes

Slc:ddY mice that received a single intraperitoneal injection of 200 mg/kg streptozotocin (STZ) were examined for persistency of diabetes (changes of indigenous bacterial floras, and bacterial translocation. Significant diabetes (increase in plasma glucose and decrease in insulin) was recognized 2 weeks after the injection, and persisted for 12 weeks. The numbers of aerobic gram-negative bacilli, staphylococci (including micrococci), and streptococci in caecal and oral floras were significantly increased, but the numbers of anaerobic bacteria in caecal flora were hardly changed. Bacterial translocation of indigenous bacteria to the mesenteric lymph node, lung, or kidney was detectable in some mice 2 weeks after the injection. The incidence of bacterial translocation in these STZ-treated mice then increased; infection caused by several organisms, e.g., Klebsiella pneumoniae, Staphylococcus epidermidis, streptococci, or Lactobacillus sp., occurred in lung, liver, spleen, kidneys, and mesenteric lymph node. No indigenous bacteria were cultured from these organs of control mice. This endogenous infection may have been due to the overpopulation of several bacterial strains caused by disruption of indigenous floras along with depression of immunological function.

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