Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo

AbstractIntroduction: Breeding profiles at the periparturient stage in red foxes which mated naturally or were subjected to artificial insemination were retrospectively surveyed using 130 vixens during their reproductive seasons of 2012–2017 in Japan. Material and Methods: Natural mating vixens were encouraged a maximum of three times with the same male, while artificial insemination was conducted using frozen-thawed semen with the bovine semen extender as a diluent. Results: With natural mating, conception rates after one, two, and three copulations were 55.8%, 68.0%, and 85.7%, respectively, showing a significant difference between the rates for one and three copulations. Conception rates with artificial insemination were 82.4%. Mean gestation periods were between 52.1 and 53.3 days in all groups. Mean litter sizes were 3.7–4.3 cubs with natural mating, and 4.4 cubs with artificial insemination. Although some sporadic and inconsistent changes in litter sizes were noted between primiparous and multiparous groups, these were of doubtful clinical importance. Conclusion: This is the first report from Japan concerning basic breeding events of red fox vixens in captivity.

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Identifikasi Bakteri dan Efektivitas Antibiotik dalam Pengencer untuk Mengontrol Pertumbuhan Bakteri pada Semen Sapi Friesian Holstein

jurnal veteriner ◽

10.19087/jveteriner.2019.20.1.140 ◽

2019 ◽

Vol 20 (1) ◽

pp. 140

Author(s):

Muttaqinullah Rabusin ◽

Andriani Andriani ◽

Raden Iis Arifiantini ◽

Ni Wayan Kurniani Karja

Keyword(s):

Semen Quality ◽

Bacterial Species ◽

Enterobacter Cloacae ◽

Bovine Semen ◽

Frozen Semen ◽

Bacterial Content ◽

Pseudomonas Diminuta ◽

Semen Extender ◽

Fresh Semen

This study was designed to investigate the presence of bacterial species in Friesian Holstein (FH) bovine semen at the time of collection, processing and to assess the efficacy of two types of antibiotics combinations; penicillin and streptomycin (PS) and gentamycin, tylosin, lincomycin and spectinomycin (GTLS) in semen extender on bacterial control and quality of semen. For this purpose, three experiments were conducted. In experiment 1, identification of bacterial content in fresh semen which collected from 5 bovine ejaculates. In experiment 2, identification of bacterial content in skimmilk-eggyolk extender which were prepared in artificial insemination center, Lembang, Bandung. In experiment 3, identification of bacterial content in frozen thawed semen. In the result, some of bacterial species were isolated from the bovine semen. The GTLS combination of antibiotics may be incorporated into a freezing extender or protocol without compromising the post-thawed semen quality of FH bull spermatozoa. Three types of bacteria were found in fresh semen; Klebsiella sp., Micrococcus sp., and Pediococcus sp.. Three types of bacteria were found in semen extender; Enterobacter cloacae, Pseudomonas diminuta and Serratia plymutica. Two types of bacteria were found in frozen semen; Enterobacter cloacae and Serratia plymutica. In conclusion, antibiotics PS and GTLS were effective for controlling the growth of bacteria in frozen semen.

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269 THE USE OF A NEW EXTENDER FOR STORING FRESH BOVINE SEMEN FOR LONG PERIODS

Reproduction Fertility and Development ◽

10.1071/rdv22n1ab269 ◽

2010 ◽

Vol 22 (1) ◽

pp. 291

Author(s):

L. G. Frers ◽

J. Hepburn ◽

K. Hogan ◽

C. Parminter ◽

L. Mc Gowan ◽

...

Keyword(s):

Ambient Temperature ◽

Storage Period ◽

Egg Yolk ◽

Bovine Semen ◽

Bull Semen ◽

Frozen Semen ◽

Long Life ◽

Semen Extender ◽

Fresh Semen

Processing bovine semen in fresh long life extender for use over 3 to 4 days after collection is a widely used technique in New Zealand (Shannon and Vishwanath 1995 Anim. Reprod. Sci. 39, 1-10). Advantages include greater use of valuable sires, transport without liquid nitrogen, and the possibility of more efficient use of sexed sorted semen. The new extender (Ext. A) also has the advantage of containing no egg yolk. This study compares this new long-life extender (Ext. A) with an existing product (Ext. B) and frozen/thawed semen. Semen from 12 different bulls was diluted to a concentration of 8 × 106 mL-1 and gradually cooled to 16°C. All samples were held at ambient temperature in the dark and motility was evaluated over a storage period of 4 days comparing the extenders. In this part of the trial Ext. A maintained motility better than Ext. B (P = 0.001) during the 4-day storage period (24 h: 90 v. 70%; 96 h: 85 v. 50%). The second part of the trial compared the conception rates (CR) in cows from the use of fresh long-term-extended semen and frozen/thawed semen. On 19 farms, 8546 cows were inseminated with fresh semen stored for 1 to 3 days and 7280 cows were inseminated with frozen semen. The overall CR at 7 to 8 weeks for the 19 farms was 73.7%. On 18 farms within the same farming group, 8498 cows received frozen semen and the CR was 71.1%. Pregnancy results were 2.6% (P = 0.001) higher CR at scanning in herds where fresh semen was used compared with the farms where only frozen/thawed semen was used (73.7 v. 71.1%). In the third part of the trial, semen from 4 different bulls were extended to 1 × 106 mL-1 in Ext. A and held at ambient temperature for 6 days prior to use for IVF. Our lab standard frozen/thawed bull semen was used as a control. Table 1 shows that semen held at ambient temperature in Ext. A for 6 days produced a similar percentage of transferable quality embryos to our IVP control frozen/thawed semen (26.9 v. 25.7%). We conclude that preserved bovine semen in fresh long-life extender for several days offers some advantages in AI and IVP programs compared with frozen semen. Table 1.Fresh semen extender (Ext.A) compared with frozen semen in IVP We appreciate the assistance of Liberty Genetics Ltd.

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Dose-Dependent Effect of Green Tea (Camellia sinensis) Extract in the Post-Thawed Fertility Indicators of frozen Bovine Semen Extender

Pakistan Journal of Zoology ◽

10.17582/journal.pjz/20181230051216 ◽

2021 ◽

Author(s):

Noor Bad Shah Khattak

Keyword(s):

Green Tea ◽

Camellia Sinensis ◽

Dependent Effect ◽

Bovine Semen ◽

Semen Extender ◽

Dose Dependent ◽

Dose Dependent Effect

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Does supplementation of vitamin C, reduced glutathione or their association in semen extender reduce oxidative stress in bovine frozen semen?

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-11293 ◽

2020 ◽

Vol 72 (1) ◽

pp. 9-17 ◽

Cited By ~ 1

Author(s):

S.C.C. Pinto ◽

D.S. Almeida ◽

M.B.R. Alves ◽

S.A. Florez-Rodriguez ◽

G.S. Abreu Júnior ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Sperm Motility ◽

Reduced Glutathione ◽

Control Group ◽

Mitochondrial Potential ◽

Semen Cryopreservation ◽

Bovine Semen ◽

Frozen Semen ◽

Semen Extender

ABSTRACT The aim of this study was to evaluate the addition of vitamin C, reduced glutathione and the association thereof to the bovine semen cryopreservation extender. The ejaculate from nine bulls were divided into four fractions, each corresponding to a treatment, namely: control group-semen diluted with Tris-yolk extender; vitamin C group-semen diluted in Tris-yolk extender supplemented with vitamin C (2.5mmol/mL); glutathione group-semen diluted in Tris-yolk extender supplemented with reduced glutathione (2.5mmol/mL) and associated group-semen diluted in Tris-yolk extender supplemented with vitamin C (1.25mmol/mL) and reduced glutathione (1.25mmol/mL). Afterwards, the semen was packed into French straws and submitted to cryopreservation using automated equipment. After cryopreservation, the semen was thawed and evaluated considering sperm motility, morphology, plasma membrane, acrosome, mitochondrial potential and oxidative stress, as well as the thermo resistance test. Extender’s supplementation with the association of vitamin C and reduced glutathione showed benefic effects on sperm motility and preservation of plasma and acrosomal membranes during semen cryopreservation, being also the group that showed higher values of reactive oxygen species. Thus, the association of both antioxidants contributed to the preservation of sperm cells in every analyzed characteristic, suggesting its use on bovine semen cryopreservation.

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