Folate conjugated hyaluronic acid coated alginate nanogels encapsulated oxaliplatin enhance antitumor and apoptosis efficacy on colorectal cancer cells (HT29 cell line)

In 2020, an estimated 19.3 million new cancer cases and nearly 10 million cancer deaths have occurred worldwide, with colorectal cancer ranking as the third most frequently diagnosed (10.0%). Several attempts have been conducted against cancer, including surgery, radiation, monoclonal antibodies, and chemotherapy. Many people choose natural products as alternatives against cancer. These products will not only help in human life preservation but also work as a source of up-to-date information, leading people away from incorrect information. We discuss the current status, distribution, and future implications of protecting populations with natural products as an alternative against colorectal cancer in Indonesia. Thirty-eight studies were included in this review for data extraction. The distribution of natural products in Indonesia that have potential activity against colorectal cancer cells was predominated by terpenoids, followed by phytosterols, phenolics, alkaloids, and polyisoprenoids. The type of cell line utilized in the cytotoxic activity analysis of natural products was the WiDr cell line, followed by HT-29 cells and HCT-116 cells. This review showed that MTT in vitro assay is a general method used to analyze the cytotoxic activity of a natural product against colorectal cancer cells, followed by other in vitro and in vivo methods. The systematic review provided predictions for several secondary metabolites to be utilized as an alternative treatment against colorectal cancer in Indonesia. It also might be a candidate for a future co-chemotherapy agent in safety, quality, and standardization. In addition, computational methods are being developed to predict the drug-likeness of compounds, thus, drug discovery is already on the road towards electronic research and development.

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Hyaluronic acid synthase 2 promotes malignant phenotypes of colorectal cancer cells through transforming growth factor beta signaling

Cancer Science ◽

10.1111/cas.14070 ◽

2019 ◽

Vol 110 (7) ◽

pp. 2226-2236 ◽

Cited By ~ 4

Author(s):

Young‐Heon Kim ◽

Seung Bum Lee ◽

Sehwan Shim ◽

Areumnuri Kim ◽

Ji‐Hye Park ◽

...

Keyword(s):

Colorectal Cancer ◽

Hyaluronic Acid ◽

Growth Factor ◽

Cancer Cells ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Hyaluronic Acid Synthase ◽

Colorectal Cancer Cells ◽

Growth Factor Beta

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A gold-nanodot-decorated hollow carbon nanosphere based nanoplatform for intracellular miRNA imaging in colorectal cancer cells

Chemical Communications ◽

10.1039/c9cc06674h ◽

2019 ◽

Vol 55 (82) ◽

pp. 12352-12355 ◽

Cited By ~ 1

Author(s):

Heng Xiao ◽

Huiling Fan ◽

Luming Xu ◽

Zhiyin Pei ◽

Shijun Lei ◽

...

Keyword(s):

Colorectal Cancer ◽

Hyaluronic Acid ◽

Cancer Cells ◽

Living Cells ◽

Carbon Nanospheres ◽

Colorectal Cancer Cells ◽

Hollow Carbon

We report a new biofunctionalized nanoplatform based on hyaluronic acid-coated gold-nano-dot-decorated hollow carbon nanospheres (AuHCNs-HA) for microRNA imaging in living cells.

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Assessment of the Antineoplastic Effects of the Investigational Agent PAX2M as C-MYC Onco-Protein Blocking Agent in Human Colorectal Cancer Cells Model Sw480 Cell Line

Indian Journal of Public Health Research & Development ◽

10.5958/0976-5506.2019.00749.6 ◽

2019 ◽

Vol 10 (4) ◽

pp. 519

Author(s):

Sarah Mazin Naeem ◽

Hussein A. Abdulkadhim ◽

Kifah J. Shaker ◽

Hamid N. Obied

Keyword(s):

Colorectal Cancer ◽

Cancer Cells ◽

Cell Line ◽

Blocking Agent ◽

Sw480 Cell ◽

Human Colorectal Cancer ◽

Investigational Agent ◽

Sw480 Cell Line ◽

Colorectal Cancer Cells ◽

Human Colorectal Cancer Cells

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Effect of the Cellular Extract of Co-cultured Lactobacillus Casei on BAX and Human β-Defensin 2 Genes Expression in HT29 Cells

Quarterly of Horizon of Medical Sciences ◽

10.32598/hms.26.4.3277.1 ◽

2020 ◽

Vol 26 (4) ◽

pp. 364-381

Author(s):

Mina Yavari ◽

◽

Changiz Ahmadizadeh ◽

Keyword(s):

Cancer Cells ◽

Cell Line ◽

Lactobacillus Casei ◽

Ht29 Cell ◽

Resistant Bacteria ◽

Dna Ladder ◽

Dapi Staining ◽

Ht29 Cells ◽

Cellular Extract ◽

Ht29 Cell Line

Aims: Defensins are cysteine-rich antimicrobial cationic peptides and BAX is a proapoptotic gene that can cause cell death. This study aimed to investigate the effect of cellular extract of co-cultured Lactobacillus casei on the expression of BAX and human β-defensin 2 (hBD-2) genes in HT29 cells. Methods & Materials: This experimental study was conducted in the Research Center for Pharmaceutical Nanotechnology of Tabriz University of Medical Sciences in 2017. The HT29 cell line was obtained from the Pasteur Institute of Iran, and cells were assessed using Microculture Tetrazolium Test (MTT) after culturing. DNA was extracted from the treated cells, and then the DNA ladder assay was carried out. After preparing cDNA, the expression levels of BAX and hBD-2 genes in the HT29 cell line were measured using a real-time Polymerase Chain Reaction (PCR) method. Findings: The results of the MTT assay indicated that Lactobacillus casei inhibited the proliferation of HT29 cells and induced apoptosis in these cells. Results of DAPI staining and DNA ladder assay obtained from treating HT29 cells by Lactobacillus casei showed qualitative changes in cell apoptosis. Moreover, realtime PCR results indicated that Lactobacillus casei bacteria significantly increased the expression of the hBD-2 gene in HT29 colon cancer cells within 12-24 hours (P= 0.023), while BAX gene expression showed no significant change in the first 24 hours (P= 0.37). Conclusion: The extract of Lactobacillus casei can be used to stimulate cancer cells to produce β-defensins, inhibit pathogens, prevent the stimulation of cellular signaling, and fight antibiotic-resistant bacteria.

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ABCB1 and ABCG2 restricts the efficacy of gedatolisib (PF-05212384), a PI3K inhibitor in colorectal cancer cells

Cancer Cell International ◽

10.1186/s12935-021-01800-7 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Changfu Liu ◽

Wenge Xing ◽

Haipeng Yu ◽

Weihao Zhang ◽

Tongguo Si

Keyword(s):

Colorectal Cancer ◽

Atpase Activity ◽

Cancer Cells ◽

Cell Line ◽

Abc Transporters ◽

Expression Level ◽

Colorectal Cancer Cell Line ◽

Colorectal Cancer Cells ◽

Mtt Assays

Abstract Background Overexpression of ABC transporters is a big challenge on cancer therapy which will lead cancer cells resistance to a series of anticancer drugs. Gedatolisib is a dual PI3K and mTOR inhibitor which is under clinical evaluation for multiple types of malignancies, including colorectal cancer. The growth inhibitory effects of gedatolisib on colorectal cancer cells have been specifically studied. However, the role of ABC transporters on gedatolisib resistance remained unclear. In present study, we illustrated the role of ABC transporters on gedatolisib resistance in colorectal cancer cells. Methods Cell viability investigations of gedatolisib on colorectal cancer cells were determined by MTT assays. The verapamil and Ko143 reversal studies were determined by MTT assays as well. ABCB1 and/or ABCG2 siRNA interference assays were conducted to verify the role of ABCB1- and ABCG2-overexpression on gedatolisib resistance. The accumulation assays of gedatolisib were conducted using tritium-labeled paclitaxel and mitoxantrone. The effects of gedatolisib on ATPase activity of ABCB1 or ABCG2 were conducted using PREDEASY ATPase Kits. The expression level of ABCB1 and ABCG2 after gedatolisib treatment were conducted by Western blotting and immunofluorescence assays. The well-docked position of gedatolisib with crystal structure of ABCB1 and ABCG2 were simulated by Autodock vina software. One-way ANOVA was used for the statistics analysis. Results Gedatolisib competitively increased the accumulation of tritium-labeled substrate-drugs in both ABCB1- and ABCG2-overexpression colorectal cancer cells. Moreover, gedatolisib significantly increased the protein expression level of ABCB1 and ABCG2 in colorectal cancer cells. In addition, gedatolisib remarkably simulated the ATPase activity of both ABCB1 and ABCG2, suggesting that gedatolisib is a substrate drug of both ABCB1 and ABCG2 transporters. Furthermore, a gedatolisib-resistance colorectal cancer cell line, SW620/GEDA, was selected by increasingly treatment with gedatolisib to SW620 cells. The SW620/GEDA cell line was proved to resistant to gedatolisib and a series of chemotherapeutic drugs, except cisplatin. The ABCB1 and ABCG2 were observed overexpression in SW620/GEDA cell line. Conclusions These findings suggest that overexpression of ABCB1 and ABCG2 may restrict the efficacy of gedatolisib in colorectal cancer cells, while co-administration with ABC transporter inhibitors may improve the potency of gedatolisib.

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A Comparison of the Anti-Cancer Effects of Free and PLGA-PAA Encapsulated Hydroxytyrosol on the HT-29 Colorectal Cancer Cell Line

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520621666210308095712 ◽

2021 ◽

Vol 21 ◽

Author(s):

Nasrin S. Sani ◽

Habib Onsori ◽

Somayeh Akrami ◽

Mohammad Rahmati

Keyword(s):

Colorectal Cancer ◽

Cancer Cells ◽

Cell Line ◽

Cancer Cell ◽

Cancer Cell Line ◽

Colorectal Cancer Cell ◽

Colorectal Cancer Cell Line ◽

Cycle Arrest ◽

Anti Cancer ◽

Colorectal Cancer Cells

Background: Hydroxytyrosol is one of the phenolic compounds of olive oil and can induce anti-cancer effects on the colorectal cancer cells. Objective: The aim of the present study was to evaluate the free hydroxytyrosol and nano-capsulated hydroxytyrosol effects on the cell cycle arrest in HT-29 colorectal cancer cell line. Methods: The nano-capsulated hydroxytyrosol was synthesized in poly lactide-co-glycolide-co-polyacrylic acid (PLGA-PAA) copolymer. MTT assay was performed to evaluate the anti- proliferative and anti-tumor effects of the free hydroxytyrosol and nano-capsulated hydroxytyrosol. Finally, the relative expression of CDKN1A, CDKN1B and CCND1 genes was evaluated in the control and treated colorectal cancer cells by using Real-Time PCR. Results: The obtained results from the MTT assay showed that the cytotoxic effects of the nano-capsulated hydroxytyrosol on the colorectal cancer cell line (IC50= 6PPM) was significantly more than free hydroxytyrosol (IC50= 12PPM) after 72h. Also, nano-capsulated hydroxytyrosol showed more significant effects on the up-regulation of CDKN1A and CDKN1B genes, and down-regulation of the CCND1 gene in the colorectal cancer cells. Conclusion: In conclusion, the present study showed that the hydroxytyrosol led to die the colorectal cancer cell through the cell cycle arrest. Also, the PLGA-PAA copolymer dramatically caused to increase the cytotoxic effects of the hydroxytyrosol on the colorectal cancer cells.

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Scaphium affine Ethanol Extract Induces Anoikis by Regulating the EGFR/Akt Pathway in HCT116 Colorectal Cancer Cells

Frontiers in Oncology ◽

10.3389/fonc.2021.621346 ◽

2021 ◽

Vol 11 ◽

Author(s):

Hye Won Kawk ◽

Gun-He Nam ◽

Myeong Jin Kim ◽

Sang-Yong Kim ◽

Young-Min Kim

Keyword(s):

Colorectal Cancer ◽

Cancer Cells ◽

Cell Line ◽

Hct116 Cell ◽

Akt Pathway ◽

Dependent Manner ◽

Anticancer Effects ◽

Colorectal Cancer Cells ◽

Dose Dependent

Scaphium affine ethanol extracts (SAE) is a species that has been shown to contain various physiological effects; however, its anticancer effects have yet to be revealed. We qualitatively evaluated β-sitosterol in SAE through high-performance liquid chromatography (HPLC). The cytotoxicity in HCT116 and HT29 colorectal cancer cells and CCD841 normal colon cells was confirmed through WST-1 assays. Selective cytotoxicity was observed in colorectal cancer cells, with greater cytotoxicity demonstrated in the HCT116 cell line. As such, the HCT116 colorectal cell line was selected for subsequent experiments. After HCT116 cells were treated with SAE, it was confirmed that the apoptosis rate was increased in a SAE dose-dependent manner through Annexin V assay. SAE further showed dose-dependent suppression of invasion through invasion assays. Anoikis induction through the EGFR/Akt pathway in HCT116 colorectal cancer cells was confirmed by Western blotting. The tumor suppressive effects of SAE was assessed in vivo using a xenograft model of human HCT116 colorectal cancer cells. As a result, we confirmed that SAE decreased tumor size in a dose-dependent manner and that p-EGFR and cleaved-caspase 3 in tumors were also regulated in a dose-dependent manner. This study showed that SAE, by containing β-sitosterol with proven anticancer effects, induces anoikis through the EGFR/Akt pathway in HCT116 colorectal cancer cells both in vitro and in vivo.

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