Suppression of apoptotic signaling in rat hepatocytes by non-dioxin-like polychlorinated biphenyls depends on the receptors CAR and PXR

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

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High-resolution scanning electron microscopy (HRSEM) of mitochondria from various rat tissues

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102158 ◽

1988 ◽

Vol 46 ◽

pp. 14-15

Author(s):

P.J. Lea ◽

M.J. Hollenberg

Keyword(s):

Electron Microscopy ◽

Pigment Epithelium ◽

Retinal Pigment ◽

Rat Hepatocytes ◽

Three Dimensions ◽

Objective Lens ◽

Rat Tissues ◽

Thin Sections ◽

Reconstruction Methods ◽

Scanning Electron

Our current understanding of mitochondrial ultrastructure has been derived primarily from thin sections using transmission electron microscopy (TEM). This information has been extrapolated into three dimensions by artist's impressions (1) or serial sectioning techniques in combination with computer processing (2). The resolution of serial reconstruction methods is limited by section thickness whereas artist's impressions have obvious disadvantages.In contrast, the new techniques of HRSEM used in this study (3) offer the opportunity to view simultaneously both the internal and external structure of mitochondria directly in three dimensions and in detail.The tridimensional ultrastructure of mitochondria from rat hepatocytes, retinal (retinal pigment epithelium), renal (proximal convoluted tubule) and adrenal cortex cells were studied by HRSEM. The specimens were prepared by aldehyde-osmium fixation in combination with freeze cleavage followed by partial extraction of cytosol with a weak solution of osmium tetroxide (4). The specimens were examined with a Hitachi S-570 scanning electron microscope, resolution better than 30 nm, where the secondary electron detector is located in the column directly above the specimen inserted within the objective lens.

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Morphological and biochemical analyses of changes in rat hepatocytes related to wine and ethanol consumption

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111276 ◽

1984 ◽

Vol 42 ◽

pp. 232-233

Author(s):

S.M. Geyer ◽

C.L. Mendenhall ◽

J.T. Hung ◽

E.L. Cardell ◽

R.L. Drake ◽

...

Keyword(s):

Electron Microscopy ◽

Endoplasmic Reticulum ◽

Enzyme Activity ◽

Malic Enzyme ◽

Smooth Endoplasmic Reticulum ◽

Rat Hepatocytes ◽

Mature Male ◽

Treatment Groups ◽

Malic Enzyme Activity ◽

Biochemical Analyses

Thirty-three mature male Holtzman rats were randomly placed in 3 treatment groups: Controls (C); Ethanolics (E); and Wine drinkers (W). The animals were fed synthetic diets (Lieber type) with ethanol or wine substituted isocalorically for carbohydrates in the diet of E and W groups, respectively. W received a volume of wine which provided the same gram quantity of alcohol consumed by E. The animals were sacrificed by decapitation after 6 weeks and the livers processed for quantitative triglycerides (T3), proteins, malic enzyme activity (MEA), light microscopy (LM) and electron microscopy (EM). Morphometric analysis of randomly selected LM and EM micrographs was performed to determine organellar changes in centrilobular (CV) and periportal (PV) regions of the liver. This analysis (Table 1) showed that hepatocytes from E were larger than those in C and W groups. Smooth endoplasmic reticulum decreased in E and increased in W compared to C values.

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