Modulation of cytochrome P450 1A1 expression and activity in intestinal Caco-2 cells by components of Ginkgo biloba-based dietary supplements

2009 ◽  
Vol 189 ◽  
pp. S73
Author(s):  
Laurence Ribonnet ◽  
Alfons Callebaut ◽  
Marie-Louise Scippo ◽  
Marc Muller ◽  
Johan Robbens ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Dietary Supplements ◽  
Ginkgo Biloba ◽  
Cytochrome P450 1A1 ◽  
Expression And Activity

scholarly journals Regulation of Human Cytochrome P4501A1 (hCYP1A1): A Plausible Target for Chemoprevention?

2016 ◽  
Vol 2016 ◽  
pp. 1-17 ◽  
Author(s):  
Rebeca Santes-Palacios ◽  
Diego Ornelas-Ayala ◽  
Noel Cabañas ◽  
Ana Marroquín-Pérez ◽  
Alexis Hernández-Magaña ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 1A1 ◽  
Functional Characteristics ◽  
Cytochrome P4501a1 ◽  
Important Protein ◽  
Physiological Processes ◽  
Human Cytochrome ◽  
Object Of Study ◽  
Expression And Activity ◽  
Rule Out

Human cytochrome P450 1A1 (hCYP1A1) has been an object of study due to its role in precarcinogen metabolism; for this reason it is relevant to know more in depth the mechanisms that rule out its expression and activity, which make this enzyme a target for the development of novel chemiopreventive agents. The aim of this work is to review the origin, regulation, and structural and functional characteristics of CYP1A1 letting us understand its role in the bioactivation of precarcinogen and the consequences of its modulation in other physiological processes, as well as guide us in the study of this important protein.

Molecular Regulation of Cytochrome P450 1A1 Induction By Hyperoxia in Human Pulmonary Cells

PEDIATRICS ◽  
2016 ◽  
Vol 137 (Supplement 3) ◽  
pp. 507A-507A
Author(s):  
Sudeepta K Basu ◽  
Renjithkumar Kalikkot Thekkeveedu ◽  
Chun Chu ◽  
Paramhamsa Maturu ◽  
Weiwu Jiang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Molecular Regulation ◽  
Cytochrome P450 1A1 ◽  
Pulmonary Cells

Chicken Egg Yolk as an Excellent Source of Highly Specific Antibodies Against Cytochromes P450

2004 ◽  
Vol 69 (3) ◽  
pp. 659-673 ◽  
Author(s):  
Petr Hodek ◽  
Tomáš Koblas ◽  
Helena Rýdlová ◽  
Božena Kubíčková ◽  
Miroslav Šulc ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochromes P450 ◽  
Egg Yolk ◽  
Good Alternative ◽  
Invasive Technique ◽  
Cytochrome P450 1A1 ◽  
Orconectes Limosus ◽  
Chicken Egg ◽  
Cytochrome P450 2B4 ◽  
Human Livers

Using chicken antibodies IgY (purified from egg yolks) against mammalian cytochromes P450 and by means of cytochrome P450 marker substrates, we found for the first time the presence of hepatopancreatic cytochrome P450 in crayfishOrconectes limosus(an inducible cytochrome P450 2B-like enzyme) and we were able to detect and quantify cytochrome P450 1A1 in microsomes of human livers. Expression levels of cytochrome P450 1A1 in human livers constituted less than 0.6% of the total hepatic cytochrome P450 complement. The results obtained in our study are clear examples that chicken IgY are suitable for cytochrome P450 detection and quantification. Due to the evolutionary distance, chicken IgY reacts with more epitopes on a mammalian antigen, which gives an amplification of the signal. Moreover, this approach offers many advantages over common mammalian antibody production since chicken egg is an abundant source of antibodies (about 100 mg IgY/yolk) and the egg collection is a non-invasive technique. In the case of antibodies against cytochrome P450 2B4, we documented fast and steady production of highly specific immunoglobulins. Thus, chicken antibodies should be considered as a good alternative to and/or superior substitute for conventional polyclonal antibody produced in mammals.

scholarly journals A Health Risk Assessment of Lead and Other Metals in Pharmaceutical Herbal Products and Dietary Supplements Containing Ginkgo biloba in the Mexico City Metropolitan Area

2021 ◽  
Vol 18 (16) ◽  
pp. 8285
Author(s):  
Patricia Rojas ◽  
Elizabeth Ruiz-Sánchez ◽  
Camilo Ríos ◽  
Ángel Ruiz-Chow ◽  
Aldo A. Reséndiz-Albor
Keyword(s):  
Health Risk ◽  
Dietary Supplements ◽  
Mexico City ◽  
Metropolitan Area ◽  
Ginkgo Biloba ◽  
Graphite Furnace ◽  
Human Health Risk ◽  
Daily Intake ◽  
Herbal Remedies ◽  
Herbal Products

The use of the medicinal plant Ginkgo biloba has increased worldwide. However, G. biloba is capable of assimilating both essential and toxic metals, and the ingestion of contaminated products can cause damage to health. The aim of this study was to investigate the safety of manganese (Mn), copper (Cu), lead (Pb), arsenic (As), and cadmium (Cd) in 26 items containing Ginkgo biloba (pharmaceutical herbal products, dietary supplements, and traditional herbal remedies) purchased in the metropolitan area of Mexico City. Metal analysis was performed using a graphite furnace atomic absorption spectrometer. All of the products were contaminated with Pb, 54% of them with As, and 81% with Cd. The lowest values of Pb, As, and Cd were detected in pharmaceutical herbal products > dietary supplements > traditional herbal remedies. The daily intake dose (DID) of pharmaceutical herbal products was within the established limits for the five metals. Dietary supplements and traditional herbal remedies exceeded the DID limits for Pb. The hazard quotients estimation and non-carcinogenic cumulative hazard estimation index for Mn, As, and Cd indicated no human health risk. Our results suggest that products containing G. biloba for sale in Mexico are not a health risk.

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