Toxicity of zinc oxide nanoparticles towards a fish and mammalian cell line

Nanoparticles (NPs) have unique properties compared to their bulk counterparts, and they have potentials for various applications in many fields of life science. Green-synthesized NPs have garnered considerable interest due to their inherent features such as rapidity, eco-friendliness and cost-effectiveness. Zinc oxide nanoparticles (ZnO NPs) were synthesized using an aqueous extract of Kalanchoe blossfeldiana as a reducing agent. The resulting nanoparticles were characterized via X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, photoluminescence (PL), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). The antimicrobial potential of the synthesized ZnO NPs against bacterial and fungal strains was examined by the disk diffusion method, and they showed a promising antibacterial and antifungal potential. The catalytic activity of the synthesized ZnO NPs in reducing methylene blue (MB) and eosin was studied via UV-Vis spectroscopy. The decolorization percentages of the MB and Eosin Y dyes were 84% and 94%, respectively, which indicate an efficient degradation of the ZnO NPs. In addition, the cytotoxic activity of the ZnO NPs on the HeLa cell line was evaluated via in vitro assay. The MTT assay results demonstrate a potent cytotoxic effect of the ZnO NPs against the HeLa cancer cell line.

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Effect of topotycan and zinc oxide nanoparticles combination on cytotoxicity and P53 gene expression against breast cancer (MCF-7) cell line

Koomesh journal ◽

10.29252/koomesh.22.1.192 ◽

2020 ◽

Vol 22 (1) ◽

pp. 192-197

Author(s):

Alireza Sharifian ◽

Fahimeh Baghbani-Arani ◽

Hasan Sahebjami ◽

◽

...

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Zinc Oxide ◽

Cell Line ◽

Zinc Oxide Nanoparticles ◽

P53 Gene ◽

Oxide Nanoparticles ◽

Mcf 7

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Pharmacological Study on Activity of Silymarin and/or Zinc Oxide Nanoparticles against Hepatic Dysfunction Using Cell Line HepG2

Virology & Immunology Journal ◽

10.23880/vij-16000218 ◽

2019 ◽

Vol 3 (3) ◽

Author(s):

Sameh S Gad

Keyword(s):

Zinc Oxide ◽

Cell Line ◽

Zinc Oxide Nanoparticles ◽

Hepatic Dysfunction ◽

Pharmacological Study ◽

Oxide Nanoparticles

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Biosynthesis Zinc Oxide Nanoparticles Using Senna Occidentalis Leaf Extract and Evaluation of their Cytotoxic Effect on SW480 Colon Cancer Cell Line

10.21203/rs.3.rs-1215740/v1 ◽

2022 ◽

Author(s):

Nidhi chaudhary ◽

Manish Pratap Singh ◽

Preeti Sirohi ◽

Shadma Afzal ◽

Nand K. Singh

Keyword(s):

Colon Cancer ◽

Zinc Oxide ◽

Cell Line ◽

Cancer Cell ◽

Zinc Oxide Nanoparticles ◽

Cancer Cell Line ◽

Colon Cancer Cell Line ◽

Colon Cancer Cell ◽

Oxide Nanoparticles ◽

Zno Nps

Abstract ObjectiveIn the present study, we green synthesized ZnO NPs (zinc oxide nanoparticles) from Senna occidentalis leaf extract which were subsequently assessed for their cytotoxic and antioxidant activity on colon cancer SW480 cell line.Results Zinc oxide nanoparticles were characterized by using UV-Vis spectroscopy, X-ray diffractometer (XRD), Particle size analyzer (PSA), Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FE-SEM), Energy-dispersive X-ray (EDS) and atomic force microscope (AFM) analysis. The PSA, XRD and AFM showed 20-50 nm size and nearly cuboidal and irregular shaped of the ZnO NPs. The synthesized ZnO nanoparticles were evaluated for their anticancer activity on human colon cancer cell line (SW480) by using MTT and neutral red uptake assay. The SW480 colon cancer cells were treated with various concentrations of ZnO NPs in the range of 20–100 µg/ml for 2 hrs. The result showed that ZnO NPs could reduce cell viability of SW480 cells up to 50% at the concentration of 100 µg/ml and induce membrane leakage in a concentration dependent manner.ConclusionThe anticancer activity of zinc oxide nanoparticles has showed that these can be used as effective anti-cancer agent against colon cancer cell lines (SW480).

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Green Synthesis of Zinc Oxide Nanoparticles (ZnO NPs) using Aqueous Extract of Tagetes erecta flower and Evaluation of its Antioxidant, Antimicrobial, and Cytotoxic activities on HeLa cell line.

Current Biotechnology ◽

10.2174/2211550109999201202123939 ◽

2020 ◽

Vol 09 ◽

Author(s):

Akila Ilangovan ◽

Akshaya Venkatramanan ◽

Pakutharivu Thangarajan ◽

Anitha Saravanan ◽

Sowndarya Rajendran ◽

...

Keyword(s):

Antimicrobial Activity ◽

Zinc Oxide ◽

Cell Line ◽

Cancer Cell ◽

Crude Extract ◽

Zinc Oxide Nanoparticles ◽

Cancer Cell Line ◽

Tagetes Erecta ◽

Oxide Nanoparticles ◽

Zno Nps

Background: The Zinc oxide Nanoparticles (ZnO NPs) were synthesized successfully by using Tagetes erecta flower aqueous extract and evaluated for their antioxidant potential, antimicrobial and cytotoxic potential. Methods: Phytochemical screening of aqueous crude extract and synthesized ZnO NPs of Tagetes erecta flower revealed the presence of alkaloids, flavonoids, carbohydrates, amino acids, tannins, and proteins, etc. The characterization was done by various spectral analyses. Invitro antioxidant activities of synthesized ZnO NPs were found to possess concentrationdependent free radical scavenging activity was carried on different free radicals i.e. DPPH and ABTS. Antimicrobial activity of synthesized ZnO NPs was done by agar well diffusion method and compared with control ampicillin, while cytotoxic effects were determined by MTT assay against human cervical cancer cell line. Results: UV-Visible spectra were conducted to confirm the synthesis of ZnO NPs and peak obtained at 364.15nm. X-ray analysis confirmed the crystalline nature of the nanoparticles and the average size of the nanoparticles was 30-50nm and was spherical shape analyzed by SEM. The synthesized ZnO NPs showed antimicrobial activity against all tested microorganisms and maximum inhibition zone was found against E.coli in gram-negative and S.aureus in gram-positive bacteria. Synthesized ZnO NPs were showed 50% cell viability at 26.53µg/ml against the HeLa cancer cell line. Conclusion: The conclusion of this study suggests both the aqueous crude extract of Tagetes erecta flower and synthesized ZnO NPs showed an excellent alternative source of antimicrobial agent also an attractive selective cytotoxic activity against HeLa tested cancer lines, offering satisfying ‘safe and cheaper’ alternatives to conventional therapy protocols.

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