scholarly journals Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4198
Author(s):  
Ali Aldalbahi ◽  
Seham Alterary ◽  
Ruba Ali Abdullrahman Almoghim ◽  
Manal A. Awad ◽  
Noura S. Aldosari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Cell Line ◽  
Zinc Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Eosin Y ◽  
Zno Nps ◽  
Vitro Assay ◽  
Vis Spectroscopy

Nanoparticles (NPs) have unique properties compared to their bulk counterparts, and they have potentials for various applications in many fields of life science. Green-synthesized NPs have garnered considerable interest due to their inherent features such as rapidity, eco-friendliness and cost-effectiveness. Zinc oxide nanoparticles (ZnO NPs) were synthesized using an aqueous extract of Kalanchoe blossfeldiana as a reducing agent. The resulting nanoparticles were characterized via X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, photoluminescence (PL), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). The antimicrobial potential of the synthesized ZnO NPs against bacterial and fungal strains was examined by the disk diffusion method, and they showed a promising antibacterial and antifungal potential. The catalytic activity of the synthesized ZnO NPs in reducing methylene blue (MB) and eosin was studied via UV-Vis spectroscopy. The decolorization percentages of the MB and Eosin Y dyes were 84% and 94%, respectively, which indicate an efficient degradation of the ZnO NPs. In addition, the cytotoxic activity of the ZnO NPs on the HeLa cell line was evaluated via in vitro assay. The MTT assay results demonstrate a potent cytotoxic effect of the ZnO NPs against the HeLa cancer cell line.

scholarly journals Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1919
Author(s):  
Elsayim Rasha ◽  
AlOthman Monerah ◽  
Alkhulaifi Manal ◽  
Ali Rehab ◽  
Doud Mohammed ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Klebsiella Pneumoniae ◽  
Zinc Oxide Nanoparticles ◽  
Acacia Nilotica ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray ◽  
Carbapenem Resistant

Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant Klebsiella pneumoniae (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of Acacia nilotica (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells.

scholarly journals Photocatalytic activity of biogenic zinc oxide nanoparticles: In vitro antimicrobial, biocompatibility, and molecular docking studies

2021 ◽  
Vol 10 (1) ◽  
pp. 1079-1091
Author(s):  
Mahboob Alam
Keyword(s):  
Zinc Oxide ◽  
Molecular Docking ◽  
Zinc Oxide Nanoparticles ◽  
Dye Degradation ◽  
Antimicrobial Properties ◽  
Morphological Properties ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Receptor Binding Site

Abstract The biogenic synthesis of zinc oxide nanoparticles (ZnO NPs) with pinecone extract (PCE) as a reducing agent and antibacterial agent was explored. The current study aims to investigate the biosynthesis of ZnO NPs and their effect on photocatalytic dye degradation and antimicrobial properties. The physical, chemical, and morphological properties of biogenic ZnO NPs synthesized using PCE were investigated using advanced spectroscopy techniques such as Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy, transmission electron microscopy (TEM) analysis, selected area electron diffraction (SAED), and X-ray diffraction (XRD) techniques. The photocatalytic degradation of methylene blue was measured spectrophotometrically using biogenic ZnO NPs as nanocatalysts, and decolonization of solution indicates dye degradation gradually as exposure duration increases. The antimicrobial properties of ZnONPs against the tested pathogenic strains were demonstrated using the disc diffusion method. The antimicrobial efficacy of ZnONPs was further explained using molecular docking analysis. Confirmation of the lowest binding energy was used to predict receptor binding site with NPs in order to understand the mechanistic approach. ZnONPs are likely to interact with pathogens via mechanical enfolding, which could be one of the major toxicity actions of ZnONPs against strains. Furthermore, the nontoxicity and biocompatibility of ZnO NPs were studied, revealing impressive hemocompatibility with red blood cells (RBCs) and no significant toxicity to Brine shrimps at lower ZnONP concentrations.

Preparation and Antibacterial Performances of Electrocatalytic Zinc Oxide Nanoparticles with Diverse Morphologies

2021 ◽  
Vol 17 (9) ◽  
pp. 1824-1829
Author(s):  
Junlin Li ◽  
Xiangfei Li ◽  
Dong Liang ◽  
Xiaojuan Zhang ◽  
Qing Lin ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Surface Area ◽  
Zinc Oxide Nanoparticles ◽  
Average Particle Size ◽  
Three Dimensional ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Average Particle ◽  
X Ray Diffraction ◽  
Zno Nps

This study exploits the potential of zinc oxide nanoparticles (ZnO-NPs) with diverse morphologies as catalysts and antibacterial agent. Spherical ZnO-NPs, rod-shaped ZnO-NPs and flower-shaped ZnO-NPs were prepared by microemulsion method, solvent heat method and hydrothermal method, respectively. The structural characterizations of samples were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM) techniques. XRD results revealed the formation of spherical ZnO-NPs, rod-shaped ZnO-NPs and flower-shaped ZnO-NPs were all wurtzite crystal structure. SEM results showed that spherical ZnO-NPs had an average particle size of 30–40 nm, rod-shaped ZnO-NPs were about 500 nm long and 100 nm wide with obvious hexagonal crystals. Flower-shaped ZnO-NPs had a three-dimensional appearance with obvious petals. Results of electrochemical HER (Hydrogen evolution reaction) experiments revealed that spherical ZnO-NPs exhibited the highest electrocatalytic activity at the lowest potential voltage due to their largest specific surface area. The antibacterial property of ZnO-NPs samples were studied by the optical density method and disc diffusion method. All samples had antibacterial effects against E. coli. and flower-shaped ZnO-NPs showed the best antibacterial activity due to the largest surface area in comparison with spherical ZnO-NPs and rod-shaped ZnO-NPs, which promised the maximum Zn2+ release as bactericide mechanism that registered in the case of different ZnO-NPs morphologies.

scholarly journals Synthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Lippia adoensis (Koseret) and Evaluation of Its Antibacterial Activity

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Meron Girma Demissie ◽  
Fedlu Kedir Sabir ◽  
Gemechu Deressa Edossa ◽  
Bedasa Abdisa Gonfa
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Lippia Adoensis

The synthesis of metal oxide nanoparticles with the use of medicinal plant extract is a promising alternative to the conventional chemical method. This work aimed to synthesize zinc oxide nanoparticles using a green approach from indigenous “Koseret” Lippia adoensis leaf extract which is an endemic medicinal plant and cultivated in home gardens of different regions of Ethiopia. The biosynthesized zinc oxide nanoparticles were characterized using thermogravimetric analysis, X-ray diffraction, scanning electron microscopy-energy dispersive spectroscopy, transmission electron microscopy, ultraviolet-visible spectroscopy, and Fourier transform infrared spectroscopy. Furthermore, this study also evaluated the antibacterial activity of the synthesized ZnO nanoparticles against clinical and standard strains of Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Enterococcus faecalis by the disc diffusion method. According to the result of this study, ZnO nanoparticles synthesized using Lippia adoensis leaf extract showed promising result against both Gram-positive and Gram-negative bacterial strains with a maximum inhibition zone of 14 mm and 12 mm, respectively, using uncalcinated form of the synthesized ZnO nanoparticles.

scholarly journals Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo

2021 ◽  
Vol 11 ◽  
Author(s):  
Elsayim Rasha ◽  
Manal M. Alkhulaifi ◽  
Monerah AlOthman ◽  
Ibrahim Khalid ◽  
Elnagar Doaa ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Aspergillus Niger ◽  
Zinc Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray ◽  
Carbapenem Resistant

Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. pneumoniae (KPC) in vitro and in vivo. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents.

Sunflower Assisted Bio-derived ZnO-NPs as an Efficient Nanocatalyst for Synthesis of Novel Quinazolines with Highly Antioxidant Activities

2021 ◽  
Author(s):  
Balaji GL ◽  
S. Mahesh ◽  
Boya Palajonnala Narasaiah ◽  
Sadegh Rostamnia ◽  
Harihara Padhy ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Antioxidant Activities ◽  
Present Report ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Green Method ◽  
Vis Spectroscopy

Abstract The present report an environmental benign route for the fabrication of Zinc Oxide nanoparticles (ZnO-NPs) using sunflower leaf aqueous extract at room temperature. This was an green method is a rapid biogenic and offers few advantages over the chemical and physical procedures, as it is an easy and fast, eco-friendly and does not involve any costly chemicals as well as hazardous chemicals, harmful solvent. The synthesized ZnO-NPs were characterized using different technique such as UV-Visible spectroscopy. The formation of ZnO-NPs was confirmed by Surface Plasmon Resonance (SRP) at 344 nm using UV-Vis spectroscopy. The leaf extract act as a source of phyto-chemicals and are the primarily responsible for the reduction and then formation of stable Zinc Oxide nanoparticles by the characteristic functional groups of extract and synthesized ZnO-NPs were identified by FTIR spectroscopy. Crystalline nature of ZnO-NPs was confirmed by powder –XRD. Size and morphology were measured by HR-TEM analysis. Stability of the nanoparticles is represented by DLS and TGA analysis. The synthesized ZnO-NPs has been found good and efficient catalyst for the synthesis of novel 1,2-dihydroquinazoline derivatives under green method via one pot reaction of 2-amino benzophenone, 1,3-diphenyl-1 H -pyrazole carbaldehydes and ammonium acetate. The synthesized compounds (4a-o) were characterized by 1 HNMR, 13 CNMR and HRMS spectra and further screened for radical scavenging activity. Among all the compounds showed good percentage of inhibition against standard ascorbic acid. The synthesized ZnO nanoparticles showed good antioxidant activity and also act as a good promising material for catalytic agent.

scholarly journals Antibacterial and Antifungal Properties of Electrospun Recycled PET Polymeric Fibers Functionalized with Zinc Oxide Nanoparticles

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3763
Author(s):  
Katherine Vázquez ◽  
Paul Vanegas ◽  
Christian Cruzat ◽  
Néstor Novoa ◽  
Ramón Arrué ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Raw Materials ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Polymeric Fibers ◽  
Recycled Pet ◽  
Antifungal Properties ◽  
Antibacterial And Antifungal

Currently, to reduce the environmental problems associated with plastic waste, methods are being sought to use this waste as raw materials in different applications, such as fibers. In addition, to improve these materials and provide different properties, nanoparticles (NPs) are incorporated. In the present work, polymeric fibers made of recycled polyethylene terephthalate (r-PET) from post-consumer water bottles, functionalized with 0%, 1.5%, 3% and 6% zinc oxide nanoparticles (ZnO-NPs) in function of r-PET weight, were elaborated to evaluate their antibacterial and antifungal characteristics. The ZnO-NPs were synthesized by the solvothermal method, obtaining particles with a mean diameter of 38.15 nm, while the fibers were obtained by electrospinning with a diameter range between 200–5000 nm. The functionalized fibers were carried out against Escherichia coli and Bacillus subtilis through the agar diffusion method, obtaining the highest inhibition halo at 6% w/w ZnO-NPs, being 26.5 mm and 34.25 mm, respectively. In addition, the same method was used to evaluate the antifungal activity of Penicillium s.p. and Fusarium graminearum, observing antifungal properties due to the presence of nanoparticles in the fibers.

scholarly journals Green Fabrication of Zinc Oxide Nanoparticles Using Phlomis Leaf Extract: Characterization and In Vitro Evaluation of Cytotoxicity and Antibacterial Properties

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6140
Author(s):  
Amal A. Alyamani ◽  
Salim Albukhaty ◽  
Salman Aloufi ◽  
Faizah A. AlMalki ◽  
Hassan Al-Karagoly ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Normal Fibroblast ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Disk Diffusion Method

Green nanoparticle synthesis is an environmentally friendly approach that uses natural solvents. It is preferred over chemical and physical techniques due to the time and energy savings. This study aimed to synthesize zinc oxide nanoparticles (ZnO NPs) through a green method that used Phlomis leaf extract as an effective reducing agent. The synthesis and characterization of ZnO NPs were confirmed by UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Dynamic light scattering (DLS), Zeta potential, and Field Emission Scanning Electron Microscope (FESEM) techniques. In vitro cytotoxicity was determined in L929 normal fibroblast cells using MTT assay. The antibacterial activity of ZnO nanoparticles was investigated using a disk-diffusion method against S. aureus and E. coli, as well as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) content concentrations. XRD results confirmed the nanoparticles’ crystalline structure. Nanoparticle sizes were found to be around 79 nm by FESEM, whereas the hydrodynamic radius of nanoparticles was estimated to be around 165 3 nm by DLS. FTIR spectra revealed the formation of ZnO bonding and surfactant molecule adsorption on the surface of ZnO NPs. It is interesting to observe that aqueous extracts of phlomis leave plant are efficient reducing agents for green synthesis of ZnO NPs in vitro, with no cytotoxic effect on L929 normal cells and a significant impact on the bacteria tested.

Size-Dependent Biological Activities of Fluorescent Organosilane-Modified Zinc Oxide Nanoparticles

2020 ◽  
Vol 16 (2) ◽  
pp. 137-152
Author(s):  
Mariana Buşilă ◽  
Aurel Tăbăcaru ◽  
Viorica Muşsat ◽  
Bogdan Ştefan Vasile ◽  
Ionela Andreea Neaşu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Zinc Oxide ◽  
Cancer Cells ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Cytotoxic Activities ◽  
Zno Nps ◽  
Size Dependent ◽  
Transmission Electron

Surface modification of zinc oxide nanoparticles (ZnO NPs) is a strategy to tune their biocompatibility. Herein we report on the synthesis of a series of fluorescent ZnO NPs modified with 2–10% (3-glycidyloxypropyl)trimethoxysilane (GPTMS) to investigate the fluorescence properties and to explore their applications in microbiology and biomedicine. The obtained ZnO NPs were characterized by X-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM) and Fourier transform infrared spectroscopy (FTIR). Size reduction occurred from ca. 13 nm in unmodified ZnO to 3–4 nm in silane-modified samples and fluorescence spectra showed size-dependent variation of the photoemission bands' intensity. The antibacterial and cytotoxic activities were investigated on Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria, and in ovarian (A2780) and prostate (PC3) cancer cells by tetrazolium/formazan-based methods. The antibacterial effect was higher for E. coli than S. aureus, while the cytotoxic activity was similar for both cancer cells and varied with the particle size. Cell death by apoptosis, and/or necrosis versus autophagy, were explored by flow cytometry using an Annexin V based-method and transmission electron microscopy (TEM). The main mechanism of ZnO NPs toxicity may involve the generation of reactive oxygen species (ROS) and the induction of apoptosis or autophagy. This work revealed the potential utility of GPTMS-modified ZnO NPs in the treatment of bacterial infection and cancer.

scholarly journals Cytotoxic effects of zinc oxide nanoparticles on cyanobacteriumSpirulina (Arthrospira) platensis

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4682 ◽  
Author(s):  
Sinouvassane Djearamane ◽  
Yang Mooi Lim ◽  
Ling Shing Wong ◽  
Poh Foong Lee
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Cell Viability ◽  
Aquatic Environment ◽  
Zinc Oxide Nanoparticles ◽  
Algal Biomass ◽  
Time Dependent ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Cytotoxic Effects

BackgroundThe extensive usage of zinc oxide nanoparticles (ZnO NPs) in industrial and consumer products raises the risk of releasing their residues into the aquatic environment. The presence of ZnO NPs in the aquatic environment could potentially cause cytotoxic effects on aquatic organisms. Thus, investigating the cytotoxic effects of ZnO NPs on microalgae, which form the base for the food web of aquatic biota, is essential to gain information regarding the ecotoxicological effects of metallic oxide nanoparticles in the aquatic ecosystem. Therefore, the present study has investigated in detail the assorted cytotoxic effects of ZnO NPs onS. platensisusing various concentrations of ZnO NPs (10–200 mg/L) from 6 to 96 h to explore the dose- and time-dependent cytotoxic effects.MethodsThe cytotoxic effects were all assessed through quantification of loss in cell viability, reduction in biomass and decrease in photosynthetic pigments such as chlorophyll-a, carotenoids and phycocyanin. The surface interactions of nanoparticles and the subsequent morphological alterations on algal cells were examined by optical and scanning electron microscopy (SEM). The intracellular alterations of algal cells were studied using transmission electron microscopy. Furthermore, Fourier transformed infrared (FTIR) spectrum was obtained to investigate the involvement of algal surface biomolecules in surface binding of ZnO NPs on algal cells.ResultsThe treatment of ZnO NPs onS. platensisexhibited a typical concentration- and time-dependent cytotoxicity. Results showed a significant (p < 0.05) cytotoxicity from 24 h onwards for all tested concentrations of ZnO NPs. The maximum cytotoxicity on algal cells was achieved at 96 h of exposure to ZnO NPs. In comparison with control, the algal cells that interacted with 200 mg/L of ZnO NPs for 96 h showed 87.3 ± 1% loss in cell viability, 76.1 ± 1.7% reduction in algal biomass, 92.5 ± 2.2%, 76.2 ± 2.2% and 74.1 ± 3.4% decrease in chlorophyll-a, carotenoids and phycocyanin contents respectively. Our study confirmed the cytotoxicity of ZnO NPs through the algal growth inhibition with 72 h EC10and EC50values of 1.29 and 31.56 mg/L, respectively. The microscopic examinations of the algal cells that interacted with ZnO NPs showed severe cell membrane and intracellular damage. The SEM EDX spectrum of ZnO NPs treated algal biomass evidenced the surface accumulation of zinc in the biomass. Finally, the FTIR spectrum confirmed the involvement of amino, hydroxyl and carboxylic groups of algal cell wall in the surface interaction of ZnO NPs on the algal cells.DiscussionThe results showed that the treatment of ZnO NPs onS. platensistriggered substantial cytotoxicity and caused cell death. Hence,S. platensiscould be potentially used as a bioindicator for testing toxicity of ZnO NPs in aquatic environment.

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