Green Synthesis of Zinc Oxide Nanoparticles (ZnO NPs) using Aqueous Extract of Tagetes erecta flower and Evaluation of its Antioxidant, Antimicrobial, and Cytotoxic activities on HeLa cell line.

2020 ◽  
Vol 09 ◽  
Author(s):  
Akila Ilangovan ◽  
Akshaya Venkatramanan ◽  
Pakutharivu Thangarajan ◽  
Anitha Saravanan ◽  
Sowndarya Rajendran ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Cell Line ◽  
Cancer Cell ◽  
Crude Extract ◽  
Zinc Oxide Nanoparticles ◽  
Cancer Cell Line ◽  
Tagetes Erecta ◽  
Oxide Nanoparticles ◽  
Zno Nps

Background: The Zinc oxide Nanoparticles (ZnO NPs) were synthesized successfully by using Tagetes erecta flower aqueous extract and evaluated for their antioxidant potential, antimicrobial and cytotoxic potential. Methods: Phytochemical screening of aqueous crude extract and synthesized ZnO NPs of Tagetes erecta flower revealed the presence of alkaloids, flavonoids, carbohydrates, amino acids, tannins, and proteins, etc. The characterization was done by various spectral analyses. Invitro antioxidant activities of synthesized ZnO NPs were found to possess concentrationdependent free radical scavenging activity was carried on different free radicals i.e. DPPH and ABTS. Antimicrobial activity of synthesized ZnO NPs was done by agar well diffusion method and compared with control ampicillin, while cytotoxic effects were determined by MTT assay against human cervical cancer cell line. Results: UV-Visible spectra were conducted to confirm the synthesis of ZnO NPs and peak obtained at 364.15nm. X-ray analysis confirmed the crystalline nature of the nanoparticles and the average size of the nanoparticles was 30-50nm and was spherical shape analyzed by SEM. The synthesized ZnO NPs showed antimicrobial activity against all tested microorganisms and maximum inhibition zone was found against E.coli in gram-negative and S.aureus in gram-positive bacteria. Synthesized ZnO NPs were showed 50% cell viability at 26.53µg/ml against the HeLa cancer cell line. Conclusion: The conclusion of this study suggests both the aqueous crude extract of Tagetes erecta flower and synthesized ZnO NPs showed an excellent alternative source of antimicrobial agent also an attractive selective cytotoxic activity against HeLa tested cancer lines, offering satisfying ‘safe and cheaper’ alternatives to conventional therapy protocols.

scholarly journals Biosynthesis Zinc Oxide Nanoparticles Using Senna Occidentalis Leaf Extract and Evaluation of their Cytotoxic Effect on SW480 Colon Cancer Cell Line

2022 ◽  
Author(s):  
Nidhi chaudhary ◽  
Manish Pratap Singh ◽  
Preeti Sirohi ◽  
Shadma Afzal ◽  
Nand K. Singh
Keyword(s):  
Colon Cancer ◽  
Zinc Oxide ◽  
Cell Line ◽  
Cancer Cell ◽  
Zinc Oxide Nanoparticles ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Oxide Nanoparticles ◽  
Zno Nps

Abstract ObjectiveIn the present study, we green synthesized ZnO NPs (zinc oxide nanoparticles) from Senna occidentalis leaf extract which were subsequently assessed for their cytotoxic and antioxidant activity on colon cancer SW480 cell line.Results Zinc oxide nanoparticles were characterized by using UV-Vis spectroscopy, X-ray diffractometer (XRD), Particle size analyzer (PSA), Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FE-SEM), Energy-dispersive X-ray (EDS) and atomic force microscope (AFM) analysis. The PSA, XRD and AFM showed 20-50 nm size and nearly cuboidal and irregular shaped of the ZnO NPs. The synthesized ZnO nanoparticles were evaluated for their anticancer activity on human colon cancer cell line (SW480) by using MTT and neutral red uptake assay. The SW480 colon cancer cells were treated with various concentrations of ZnO NPs in the range of 20–100 µg/ml for 2 hrs. The result showed that ZnO NPs could reduce cell viability of SW480 cells up to 50% at the concentration of 100 µg/ml and induce membrane leakage in a concentration dependent manner.ConclusionThe anticancer activity of zinc oxide nanoparticles has showed that these can be used as effective anti-cancer agent against colon cancer cell lines (SW480).

scholarly journals Eco-Friendly Formulated Zinc Oxide Nanoparticles: Induction of Cell Cycle Arrest and Apoptosis in the MCF-7 Cancer Cell Line

Genes ◽  
2017 ◽  
Vol 8 (10) ◽  
pp. 281 ◽  
Author(s):  
Amin Boroumand Moghaddam ◽  
Mona Moniri ◽  
Susan Azizi ◽  
Raha Abdul Rahim ◽  
Arbakariya Bin Ariff ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Zinc Oxide ◽  
Cell Cycle Arrest ◽  
Cell Line ◽  
Cancer Cell ◽  
Zinc Oxide Nanoparticles ◽  
Cancer Cell Line ◽  
Oxide Nanoparticles ◽  
Cycle Arrest ◽  
Mcf 7

scholarly journals Plant Mediated Green Synthesis of Zinc Oxide Nanoparticles for Burn Wound Therapy

2020 ◽  
Author(s):  
Mohd Riyaz Beg ◽  
Shital Ghodinde ◽  
Vidhi Gupta
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Zinc Oxide ◽  
Green Synthesis ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Burn Wound ◽  
Zno Nps ◽  
Burn Wounds ◽  
Burn Wound Healing

In this changing world, we all are surrounded by the surmountable risk of getting injured. Amongst various risk factors, major burns are the most distressing and catastrophic. Burn wounds are not easy to heal via natural healing process and ultimately ended up with scar formation. If the degree of burn is high then the loss of tissue and its function is very common. To fasten-up the natural burn wound healing; zinc, an essential trace element is found to be very much effective. But due to its’ particle size limitation, less contact with wounded cells and tissues, and high inherent toxicity restrict its use. Needlessly, zinc is an element with dual action i.e. both antimicrobial and wound healing it is a prime choice to apply its aptitude in burn wound healing. To overcome the documented limitations zinc has converted to nanoparticle form. Zinc oxide nanoparticles, in particular, have attained ample of interest due to their unique properties and potential antimicrobial activity along with wound healing activity which makes it promising for the healing of topical burn wounds. Plant mediated green synthesis of nano-metal oxide particles is gaining a lot of significance due to its simplicity, eco-friendliness and extensive antimicrobial activity and recommended as an appealing substitute to not only physical methods but also chemical methods avoiding the use of the high rate of toxic chemicals and extreme surroundings. This study includes ZnO NPs role in burn wound healing with Phyto-mediated synthesis methods to provide evidence of their potential applications. Additionally, it provides an overview of traditional methods used for the synthesis of ZnO nanoparticles and characterization techniques to obtain information concerning the size, shape and optical properties along with toxicity and safety concern of ZnO NPs and its biomedical applications.

scholarly journals Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4198
Author(s):  
Ali Aldalbahi ◽  
Seham Alterary ◽  
Ruba Ali Abdullrahman Almoghim ◽  
Manal A. Awad ◽  
Noura S. Aldosari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Cell Line ◽  
Zinc Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Eosin Y ◽  
Zno Nps ◽  
Vitro Assay ◽  
Vis Spectroscopy

Nanoparticles (NPs) have unique properties compared to their bulk counterparts, and they have potentials for various applications in many fields of life science. Green-synthesized NPs have garnered considerable interest due to their inherent features such as rapidity, eco-friendliness and cost-effectiveness. Zinc oxide nanoparticles (ZnO NPs) were synthesized using an aqueous extract of Kalanchoe blossfeldiana as a reducing agent. The resulting nanoparticles were characterized via X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, photoluminescence (PL), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). The antimicrobial potential of the synthesized ZnO NPs against bacterial and fungal strains was examined by the disk diffusion method, and they showed a promising antibacterial and antifungal potential. The catalytic activity of the synthesized ZnO NPs in reducing methylene blue (MB) and eosin was studied via UV-Vis spectroscopy. The decolorization percentages of the MB and Eosin Y dyes were 84% and 94%, respectively, which indicate an efficient degradation of the ZnO NPs. In addition, the cytotoxic activity of the ZnO NPs on the HeLa cell line was evaluated via in vitro assay. The MTT assay results demonstrate a potent cytotoxic effect of the ZnO NPs against the HeLa cancer cell line.

scholarly journals A POTENT CYTOTOXICITY AND ANTIMICROBIAL ACTIVITY OF ZINC OXIDE NANOPARTICLES SYNTHESIZED BY LEAF OF IPOMOEA PES-CAPRAE (L.) R. BR.

2019 ◽  
pp. 111-117
Author(s):  
RAMESH A ◽  
SUNDARRAJ P ◽  
BALAMANI J
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Cell Lines ◽  
Zinc Oxide Nanoparticles ◽  
Assay Method ◽  
Oxide Nanoparticles ◽  
Visible Spectroscopy ◽  
Zno Nps ◽  
Cytotoxicity Activity ◽  
Uv Visible

Objective: The present study was conducted to investigate the cytotoxicity and antimicrobial activity of zinc oxide nanoparticles (ZnO NPs) synthesized as eco-friendly technique from the leaf extract of Ipomoea pes-caprae (L.) R. Br. against human lung adenocarcinoma (A549), brain tumor (U87) cells, and human pathogens Salmonella typhi, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, and Bacillus subtilis. Materials and Methods: The work was carried out with varying precursor (plant extract) volume to optimize the synthesis of ZnO NPs and it was confirmed by ultraviolet (UV)-visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, scanning electron microscopy, and atomic force microscope (AFM) characterization techniques and evaluate its cytotoxicity activity by 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl--tetrazolium bromide assay method, antimicrobial activity by disk diffusion method. Results: A peak at 320 nm with maximum intensity was observed at temperature of 80°C with pH of 8.0 in UV-visible spectroscopy confirmed the formation of ZnO NPs and we calculate the size of ZnO NPs from XRD data found as 15.8 nm. The FTIR analysis evaluated that the presence of different functional groups is carboxyl, amine, and phenolic compounds of leaves extract which are involved in the reduction of zinc ions and acts as capping the ZnO NPs. AFM microgram confirms that ZnO NPs were in nanorange and spherical in nature. The cytotoxicity activity of A549 and U87 cell lines treated with various concentrations of ZnO NPs showed a dose-dependent increase in cell inhibition and the half maximal inhibitory concentration value was calculated to be 7.8 μg/ml. The antibacterial activity of selected pathogens shows higher zone of inhibition. Conclusion: The present study reveals that synthesized ZnO NPs capping with various bioactive compounds present in the leaf of I. pes-caprae show promising activity of cancer cell lines and antimicrobial agents; hence, further detailed study may lead to develop at a novel phytomedicine for the anticancer and antimicrobial drugs.

Addition of Zinc Oxide Nanoparticles in Biodegradable Polymer and Evaluation of its Antimicrobial Activity

2018 ◽  
Vol 930 ◽  
pp. 230-235 ◽  
Author(s):  
Ana Paula Capelezzo ◽  
Laura Cassol Mohr ◽  
Janayne Sander Godoy ◽  
Alessandra Sgnaulin Bellei ◽  
Luciano Luiz Silva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Biodegradable Polymer ◽  
Zinc Oxide Nanoparticles ◽  
Antimicrobial Agents ◽  
Food Contamination ◽  
Good Alternative ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Good Antibacterial Activity

The food contamination for pathogenic microorganisms is a serious problem for the food security. Many actions can be taken in order to reduce this pathogenic multiplication, highlighting the use of active packaging for the food conservation, which have antimicrobial agents dispersed in its surface. The metal oxides and its nanoparticles have been shown effective additives for this purpose. With the growing concern with the environmental and the use of less aggressive materials, biodegradable polymers emerge like a good alternative. This work has the goal to investigate the effect on antimicrobial activity against Gram negative (Escherichia coli) and Gram positive (Staphylococcus aureus) bacteria when added zinc oxide nanoparticles (ZnO NPs) in concentration of 1% (wt/wt) in the biodegrable polymer Ecoflex®. The system was homogenized and proccessed in industrial extruder single screw and the polymer’s antimicrobial activity was evaluated by the agar diffusion tests and counting surviving microorganisms with the time. The results showed that the biodegradable polymer Ecoflex®with ZnO NPs exhibit good antibacterial activity.

Evaluation of the Effect of Nanoparticles Zinc Oxide/Camellia sinensis Complex on the Kidney of Rats Treated with Monosodium Glutamate: Antioxidant and Histological Approaches

2019 ◽  
Vol 20 (7) ◽  
pp. 542-550 ◽  
Author(s):  
Nahla S. El-Shenawy ◽  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Rasha A. Al-Eisa
Keyword(s):  
Zinc Oxide ◽  
Camellia Sinensis ◽  
Zinc Oxide Nanoparticles ◽  
Morphological Changes ◽  
Monosodium Glutamate ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Enzymatic Antioxidants ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are robustly used biomedicine. Moreover, no study has been conducted to explore the consequence of green synthesis of ZnO NPs with Camellia sinensis (green tea extract, GTE) on kidneys of rats treated with monosodium glutamate (MSG). Methods: Therefore, the objective of the research was designed to explore the possible defensive effect of GTE/ZnO NPs against MSG-induced renal stress investigated at redox and histopathological points. Results: The levels of urea and creatinine increased as the effect of a high dose of MSG, in addition, the myeloperoxidase and xanthine oxidase activates were elevated significantly with the high dose of MSG. The levels of non-enzymatic antioxidants (uric acid, glutathione, and thiol) were decreased sharply in MSG-treated rats as compared to the normal group. Conclusion: The data displayed that GTE/ZnO NPs reduced the effects of MSG significantly by reduction of the level peroxidation and enhancement intracellular antioxidant. These biochemical findings were supported by histopathology evaluation, which showed minor morphological changes in the kidneys of rats.

Zinc oxide nanoparticles help to enhance plant growth and alleviate abiotic stress: A review

2020 ◽  
Vol 21 ◽  
Author(s):  
Mohammad Faizan ◽  
Fangyuan Yu ◽  
Chen Chen ◽  
Ahmad Faraz ◽  
Shamsul Hayat
Keyword(s):  
Zinc Oxide ◽  
Plant Growth ◽  
Abiotic Stresses ◽  
Zinc Oxide Nanoparticles ◽  
Growth And Yield ◽  
Oxide Nanoparticles ◽  
Main Concern ◽  
Negative Effects ◽  
Zno Nps ◽  
Agricultural Yield

: Abiotic stresses arising from atmosphere change belie plant growth and yield, leading to food reduction. The cultivation of a large number of crops in the contaminated environment is a main concern of environmentalists in the present time. To get food safety, a highly developed nanotechnology is a useful tool for promoting food production and assuring sustainability. Nanotechnology helps to better production in agriculture by promoting the efficiency of inputs and reducing relevant losses. This review examines the research performed in the past to show how zinc oxide nanoparticles (ZnO-NPs) are influencing the negative effects of abiotic stresses. Application of ZnO-NPs is one of the most effectual options for considerable enhancement of agricultural yield globally under stressful conditions. ZnO-NPs can transform the agricultural and food industry with the help of several innovative tools in reversing oxidative stress symptoms induced by abiotic stresses. In addition, the effect of ZnO-NPs on physiological, biochemical, and antioxidative activities in various plants have also been examined properly. This review summarizes the current understanding and the future possibilities of plant-ZnO-NPs research.

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