Molecular and morphological identification of a reptile-associated tick, Amblyomma geoemydae (Acari: Ixodidae), infesting wild yellow-margined box turtles (Cuora flavomarginata) in northern Taiwan

2022 ◽  
pp. 101901
Author(s):  
Li-Lian Chao ◽  
Tien-Hsi Chen ◽  
Wei-Chih Lien ◽  
Esmeralda Erazo ◽  
Chien-Ming Shih
Author(s):  
W. R. Schucany ◽  
G. H. Kelsoe ◽  
V. F. Allison

Accurate estimation of the size of spheroid organelles from thin sectioned material is often necessary, as uniquely homogenous populations of organelles such as vessicles, granules, or nuclei often are critically important in the morphological identification of similar cell types. However, the difficulty in obtaining accurate diameter measurements of thin sectioned organelles is well known. This difficulty is due to the extreme tenuity of the sectioned material as compared to the size of the intact organelle. In populations where low variance is suspected the traditional method of diameter estimation has been to measure literally hundreds of profiles and to describe the “largest” as representative of the “approximate maximal diameter”.


Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).


Peanuts are plants of the tropical zone, therefore, for its growth and development, high temperature indicators are needed during a fairly long growing season. The climatic parameters of the south of Ukraine correspond to the needs of this culture, and today there is positive experience in growing peanuts in this region. In order to increase the efficiency of growing peanuts in the conditions of the Steppe zone, it is necessary to carry out genetic selection work to study and select a more adapted source material in order to create highly productive Ukrainian varieties adapted to the arid zone conditions. At the initial stage of this work, it is necessary to have clear signs and characteristics for evaluating the studied genotypes, and the existing methods for describing the characters and conducting an examination for distinctness, uniformity, and stability are incomplete and do not meet the requirements for in-depth work on private genetics and culture breeding. For our work, we used 18 peanut genotypes as a material, which make up the collection of the Institute of Oilseeds of NAASU. Studies were carried out in 2018 and 2019, in different weather conditions. All measurements, observations and statistical processing in the study of samples were carried out in accordance with generally accepted methods. When conducting a comparative analysis of the two methods for describing the signs of underground peanuts, it was found that one of the methods contains 23 signs and the other 17. The signs of the vegetative part of plants that characterize the habit and structure of the leaf predominate in both methods. We have identified and proposed for description 9 new characters in peanut plants: 2 - leaf characters (additional leaves and pubescence); 1 - the structure of beans (weight 100 beans); 1 - an economically valuable trait (the presence of nodules on the roots); 5 - flower features (size and color of the flower and border). In terms of plant habitat, Ukrainian Stepnyak and Krasnodar 13 varieties were distinguished, which had the highest height (43.8 cm) and the largest number of branches (9.3 pcs.). In addition to morphological identification features for the description and examination of distinctness, uniformity and stability, we have also studied the quantitative characteristics of underground peanuts. It was established that the height of peanut plants in the field conditions of the south of Ukraine ranged from 14 to 44 cm. The highest variety was the Ukrainian Stepnyak variety, and the shortest one was Pink large. The largest number of branches is 9.3 pcs. noted in the variety Krasnodar 13, and the smallest 4.62 pcs. - at L3. And the largest flower of 1.91 cm stood out White-pink 3. The quantitative traits we studied are characterized by continuous variability, which is due to the interaction between genes and the environment. Thus, to identify varietal variability in underground peanuts, it is necessary to use both existing methods, and in the near future, work should be done to combine them with the inclusion of new identified characters to more clearly identify the genotypes of this new valuable oilseed crop.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Parisa Soltan-Alinejad ◽  
Javad Rafinejad ◽  
Farrokh Dabiri ◽  
Piero Onorati ◽  
Olle Terenius ◽  
...  

Abstract Objectives Annually, 1.2 million humans are stung by scorpions and severely affected by their venom. Some of the scorpion species of medical importance have a similar morphology to species with low toxicity. To establish diagnostic tools for surveying scorpions, the current study was conducted to generate three mitochondrial markers, Cytochrome Oxidase I (COI gene), 12S rDNA and 16S rDNA for six species of medically important Iranian scorpions: Androctonus crassicauda, Hottentotta saulcyi, Mesobuthus caucasicus, M. eupeus, Odontobuthus doriae, and Scorpio maurus. Results Phylogenetic analyses of the obtained sequences corroborated the morphological identification. For the first time, 12S rDNA sequences are reported from Androctonus crassicauda, Hottentotta saulcyi, Mesobuthus caucasicus and M. eupeus and also the 16S rDNA sequence from Hottentotta saulcyi. We conclude that the mitochondrial markers are useful for species determination among these medically important species of scorpions.


2021 ◽  
pp. 073563312199595
Author(s):  
Te-Lien Chou ◽  
Kai-Yu Tang ◽  
Chin-Chung Tsai

Programming learning has become an essential literacy for computer science (CS) and non-CS students in the digital age. Researchers have addressed that students’ conceptions of learning influence their approaches to learning, and thus impact their learning outcomes. Therefore, we aimed to uncover students’ conceptions of programming learning (CoPL) and approaches to programming learning (APL), and analyzed the differences between CS and non-CS students. Phenomenographic analysis was adopted to analyze 31 college students (20 CS-related, and 11 not) from northern Taiwan. Results revealed six categories of CoPL hierarchically: 1. memorizing concepts, logic, and syntax, 2. computing and practicing programming writing, 3. expressing programmers’ ideas and relieving pressure, 4. applying and understanding, 5. increasing one’s knowledge and improving one’s competence, and 6. seeing in a new way. Four categories of APL were also found, namely: 1. copying from the textbook, teachers, or others, 2. rote memory, 3. multiple exploration attempts, and 4. online or offline community interactions. Furthermore, we found that most CS students held higher level CoPL (e.g., seeing in a new way) than non-CS students. However, compared with non-CS students, CS students adopted more surface approaches to learning programming, such as copying and rote memory. Implications are discussed.


Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 146
Author(s):  
Jordan Hoffman ◽  
Ilinca Ciubotariu ◽  
Limonty Simubali ◽  
Twig Mudenda ◽  
William Moss ◽  
...  

Despite dramatic reductions in malaria cases in the catchment area of Macha Hospital, Choma District, Southern Province in Zambia, prevalence has remained near 1–2% by RDT for the past several years. To investigate residual malaria transmission in the area, this study focuses on the relative abundance, foraging behavior, and phylogenetic relationships of Anopheles squamosus specimens. In 2011, higher than expected rates of anthropophily were observed among “zoophilic” An. squamosus, a species that had sporadically been found to contain Plasmodium falciparum sporozoites. The importance of An. squamosus in the region was reaffirmed in 2016 when P. falciparum sporozoites were detected in numerous An. squamosus specimens. This study analyzed Centers for Disease Control (CDC) light trap collections of adult mosquitoes from two collection schemes: one performed as part of a reactive-test-and-treat program and the second performed along a geographical transect. Morphological identification, molecular verification of anopheline species, and blood meal source were determined on individual samples. Data from these collections supported earlier studies demonstrating An. squamosus to be primarily exophagic and zoophilic, allowing them to evade current control measures. The phylogenetic relationships generated from the specimens in this study illustrate the existence of well supported clade structure among An. squamosus specimens, which further emphasizes the importance of molecular identification of vectors. The primarily exophagic behavior of An. squamosus in these collections also highlights that indoor vector control strategies will not be sufficient for elimination of malaria in southern Zambia.


2020 ◽  
Vol 20 (1) ◽  
pp. 193-200
Author(s):  
Hui Deng ◽  
Jun Li

AbstractIn recent years, with the participation of genetics and other disciplines, the controversy on the origins of the domestic chicken has returned. As the resource of primary data, archaeology plays an extremely important role in this dispute. Taking an archaeological standpoint, this paper aims to establish a set of bone morphological identification standards for domestic chicken bones unearthed at archaeological remains, beginning with the bone morphology as the most basic but also the least studied aspect. By this set of standards, we reanalyze available chicken bone materials and relevant pictorial and textual materials for domestic chicken candidate samples as mentioned by previous scholars. The results show that no confirmed domestic chicken bones have been found in China’s early to mid-Holocene remains to date; meanwhile, there is no substantial archaeological evidence to support China as the earliest place of origin of domestic chicken. Future work seeking to advance research on the origin of the domestic chicken should first pay proper attention to the archaeological background; only continuing scientific analyses and exploration on the origin of domestic chicken based on scientific morphological identification will prove the most convincing methodology.


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