Identification of conserved Mycoplasma agalactiae surface antigens by immunoproteomics

2021 ◽  
Vol 236 ◽  
pp. 110239
Author(s):  
Carla Cacciotto ◽  
Maria Filippa Addis ◽  
Daniela Pagnozzi ◽  
Elisabetta Coradduzza ◽  
Marco Pittau ◽  
...  
Keyword(s):  
Surface Antigens ◽  
Mycoplasma Agalactiae

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

III. STRUCTURE AND BIOLOGICAL PROPERTIES OF SURFACE ANTIGENS FROM GRAM-NEGATIVE BACTERIA

1963 ◽  
Vol 27 (4) ◽  
pp. 352-368 ◽  
Author(s):  
Kelsey C. Milner ◽  
Robert L. Anacker ◽  
Kazue Fukushi ◽  
Willard T. Haskins ◽  
Maurice Landy ◽  
...  
Keyword(s):  
Surface Antigens ◽  
Biological Properties ◽  
Gram Negative Bacteria ◽  
Gram Negative

scholarly journals EFFECT OF 12-O-TETRADECANOYLPHORBOL-13-ACETATE ON EXPRESSION OF CELL SURFACE ANTIGENS IN TWO SUBCLONES OF HUMAN LEUKEMIA K562 CELLS

1993 ◽  
Vol 16 (10) ◽  
pp. 1054-1056
Author(s):  
Dai SASAKI ◽  
Satoshi KOSUNAGO ◽  
Takeshi MIKAMI ◽  
Tatsuji MATSUMOTO ◽  
Masuko SUZUKI
Keyword(s):  
Cell Surface ◽  
K562 Cells ◽  
Surface Antigens ◽  
Human Leukemia ◽  
Cell Surface Antigens ◽  
Human Leukemia K562 Cells

Antibodies to neuronal surface antigens in patients with a clinical diagnosis of neurodegenerative disorder

2021 ◽  
Author(s):  
Maria Pia Giannoccaro ◽  
Matteo Gastaldi ◽  
Giovanni Rizzo ◽  
Leslie Jacobson ◽  
Veria Vacchiano ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Neurodegenerative Disorder ◽  
Surface Antigens

scholarly journals Expression of the core lipopeptide of the glycopeptidolipid surface antigens in rough mutants of Mycobacterium avium

1993 ◽  
Vol 268 (14) ◽  
pp. 10510-10516
Author(s):  
J.T. Belisle ◽  
M.R. McNeil ◽  
D. Chatterjee ◽  
J.M. Inamine ◽  
P.J. Brennan
Keyword(s):  
Mycobacterium Avium ◽  
Surface Antigens ◽  
The Core

Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs

1987 ◽  
Vol 173 (2) ◽  
pp. 628-632 ◽  
Author(s):  
David Nishioka ◽  
Donald C. Porter ◽  
James S. Trimmer ◽  
Victor D. Vacquier
Keyword(s):  
Sea Urchin ◽  
Plasma Membranes ◽  
Surface Antigens ◽  
Sea Urchin Eggs ◽  
Sperm Surface
Sign in / Sign up

Export Citation Format

Share Document