Live cold-adapted influenza A vaccine produced in Vero cell line

2004 ◽  
Vol 103 (1-2) ◽  
pp. 187-193 ◽  
Author(s):  
Julia Romanova ◽  
Dietmar Katinger ◽  
Boris Ferko ◽  
Brigitta Vcelar ◽  
Sabine Sereinig ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Line ◽  
Influenza A ◽  
Cold Adapted ◽  
Vero Cell Line

Whole Genome Sequencing and Phylogenetic Analysis of the Rabies Virus Strain Moscow 3253 Adapted to a Vero Cell Line

2020 ◽  
Vol 35 (4) ◽  
pp. 237-242
Author(s):  
Ya. M. Krasnov ◽  
Zh. V. Alkhova ◽  
S. V. Generalov ◽  
I. V. Tuchkov ◽  
E. A. Naryshkina ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Vero Cell ◽  
Whole Genome Sequencing ◽  
Cell Line ◽  
Rabies Virus ◽  
Genome Sequencing ◽  
Virus Strain ◽  
Whole Genome ◽  
Vero Cell Line ◽  
Rabies Virus Strain

scholarly journals Antibacterial Activity, Cytotoxicity, and the Mechanism of Action of Bacteriocin from Bacillus subtilis GAS101

2018 ◽  
Vol 27 (2) ◽  
pp. 186-192 ◽  
Author(s):  
Garima Sharma ◽  
Shweta Dang ◽  
Sanjay Gupta ◽  
Reema Gabrani
Keyword(s):  
Molecular Weight ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Vero Cell ◽  
Cell Line ◽  
Mode Of Action ◽  
Proteolytic Enzymes ◽  
Antibiofilm Activity ◽  
Vero Cell Line ◽  
Soil Isolate

Objective: The aim of this study was to purify and characterize bacteriocin from the soil isolate Bacillus subtilis GAS101, and to determine its antimicrobial as well as antibiofilm potential. The purified bacteriocin was further analyzed and evaluated for mammalian cell cytotoxicity and the possible mode of action. Material and Methods: Bacteriocin from B. subtilis GAS101 (an animal husbandry soil isolate) was partially purified and checked for antimicrobial and antibiofilm activity against gram-positive and gram-negative bacteria. The molecular weight of bacteriocin was determined using tricine SDS-PAGE gel. The stability of bacteriocin was investigated at various temperatures and pH levels, and its sensitivity towards 8 enzymes and 6 chemicals was determined. Cytotoxicity analysis was performed on a Vero cell line by a tetrazolium dye-based assay. Scanning electron microscopy (SEM) of bacteriocin-treated bacteria was carried out to determine the possible mode of action. Results: Bacteriocin from B. subtilis GAS101 was a potential inhibitor of both the indicator organisms (Staphylococcus epidermidis and Escherichia coli), and had a molecular weight of approximately 6.5 kDa. An in situ gel assay showed a zone of inhibition corresponding to the estimated protein band size. Bacteriocin was stable and showed antibacterial activity in broad ranges of temperature (30–121°C) and pH (2–12). It was sensitive to 4 proteolytic enzymes, which indicated its proteinaceous nature. Bacteriocin showed > 70% cell viability on the mammalian Vero cell line. SEM depicted that the bacteriocin was able to disrupt the bacterial cell membrane as its probable mode of action. Conclusion: Thermostable and pH-tolerant bacteriocin from B. subtilis GAS101, of about 6.5 kDa, showed broad-spectrum antimicrobial and antibiofilm activity.

scholarly journals ​Synthesis, Characterization and Safety Assessment of Nano Selenium and Organic Selenium for Incorporation in Lamb Feed

2021 ◽  
Author(s):  
S.S. Vajpeyee ◽  
J. Ramesh ◽  
R. Karunakaran ◽  
J. Muralidharan ◽  
V. Sankar
Keyword(s):  
Particle Size ◽  
Vero Cell ◽  
Cell Line ◽  
Inductively Coupled Plasma ◽  
The Body ◽  
Nano Particles ◽  
Dependent Manner ◽  
Organic Selenium ◽  
Vero Cell Line

Background: Selenium is an important trace mineral required by the animals. It is an integral part of antioxidant system, protecting the body against free radical injury. Nano particles attract a widespread attention due to its high bioavailability and efficacy. The current study was aimed to synthesize, characterize nano selenium and evaluate the cytotoxic effect of nano selenium and organic selenium (selenocysteine) under in vitro condition in vero cell line. Methods: Nano selenium was synthesized by wet chemical method by using sodium selenite, selenium powder, ascorbic acid and sodium hydroxide at laboratory level. In this study particle size, shape, zeta potential and selenium content were characterized by using Particle Size Analyser (PSA), Transmission Electron Microscope (TEM) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The toxicity was analysed by MTT assay against vero cell line. Result: The result revealed that selenium nano particles were spherical in shape, nano in size (less than 50 nm) and pure in nature. The nano selenium and organic selenium (selenocysteine) effectively inhibited the growth of vero cells in a dose dependent manner.

scholarly journals Investigation of antimalarial activity and cytotoxicity profiling of a Bangladeshi plant Syzygium cymosum

2020 ◽  
Vol 14 (08) ◽  
pp. 924-928
Author(s):  
Muhammad Riadul Haque Hossainey ◽  
Saiful Arefeen Sazed ◽  
Maisha Khair Nima ◽  
Mohammad Saydur Rahman ◽  
Tanvir Ashraf ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Line ◽  
Crude Extract ◽  
Antimalarial Drug ◽  
Methanolic Extract ◽  
Antimalarial Activity ◽  
Chloroform Fraction ◽  
Moderate Activity ◽  
Plant Materials ◽  
Vero Cell Line

Introduction: The persistent increase of resistance to existing antimalarials underscores the needs for new drugs. Historically, most of the successful antimalarial are derived from plants. The leaves of the S. cymosum is one of the plant materials used by traditional healers in malaria-endemic areas in Bangladesh for treatment of malaria. Here, we investigated the crude extract and its fractions against chloroquine (CQ)-sensitive 3D7, CQ-resistant Dd2, and artemisinin (ART)-resistant IPC 4912 Mondulkiri strains of Plasmodium falciparum. Methodology: The antimalarial activities were tested using HRP II based in-vitro antimalarial drug sensitivity ELISA described by WWARN and half inhibitory concentrations (IC50) were calculated by non-linear regression analysis using GraphaPad Prism. The cytotoxicity of the crude methanolic extract was assessed using the MTT assay on Vero cell line. Results: The methanolic crude extract revealed promising activity against 3D7 (IC50 6.28 µg/mL), Dd2 (IC50 13.42 µg/mL), and moderate activity against IPC 4912 Mondulkiri (IC50 17.47 µg/mL). Among the fractionated portions, the chloroform fraction revealed highest activity against IPC 4912 Mondulkiri (IC50 1.65 µg/mL) followed by Dd2 (1.73 µg/mL) and 3D7 (2.39 µg/mL). The crude methanolic extract also demonstrated good selectivity with the selectivity indices of > 15.92, > 7.45, and > 6.91 against 3D7, Dd2, and IPC 4912, respectively when tested against Vero cell line. Conclusions: This is the first report on S. cymosum for its putative antimalarial activity, and is imperative to go for further phytochemical analyses in order to investigate possible novel antimalarial drug compound(s).

scholarly journals CYTOTOXICITY IDENTIFICATION OF THE SUPERNATANT OF Manihot esculenta Crantz RHIZOME AS RIBOSOME-INACTIVATING PROTEIN TO NORMAL CELL

2010 ◽  
Vol 4 (2) ◽  
pp. 77-81
Author(s):  
Endang Astuti ◽  
Sabirin Matsjeh ◽  
Winarto Haryadi ◽  
Deni Pranowo ◽  
Nuning Sri Mulatsih
Keyword(s):  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Line ◽  
Cancer Cell ◽  
Manihot Esculenta ◽  
Normal Cell ◽  
Cytotoxic Effect ◽  
Ribosome Inactivating Protein ◽  
Manihot Esculenta Crantz ◽  
Vero Cell Line

The supernatant of Manihot esculenta Crantz rhizome has been used as an alternative remedy of infectious and cancer in Jogjakarta. Cytotoxicity assay showed that  Manihot esculenta Crantz supernatant had cytotoxic effect to cancer cell line, namely Myeloma (LC50 = 180,24 μg/mL) and HeLa (LC50 = 415,55 μg/mL), but have a little cytotoxic effect to SiHa. This research was aimed to identify cytotoxic activity of Manihot esculenta Crantz's supernatant to normal cell, particularly to human mononuclear cell and Vero cell line, and to compare to the cancer cell lines. The result showed that supernatant of M. esculenta had cytotoxic effect to normal mononuclear cell (LC50 = 564,00 μg/mL) and Vero cell line (LC50 = 686,00 μg/mL). The supernatant of M. esculenta had the highest cytotoxic activity to myeloma and relatively toxic to cervix cancer HeLa and normal cell, but less to SiHa.   Keywords: Manihot esculenta Crantz, Ribosome-inactivating Protein (RIP), cytotoxic, normal cell, cancer cell

scholarly journals Real-Time Cytotoxicity Assay of Water Extracts Hibiscus rosa-sinensis

2020 ◽  
Vol 981 ◽  
pp. 265-270
Author(s):  
Nur Husnina Iffah Bakar ◽  
Thong Chuan Lee ◽  
Ab. Rahim Mohd-Hairul ◽  
Raha Ahmad Raus ◽  
Dayang Fredalina Basri ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Line ◽  
Real Time ◽  
Water Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Cell Analysis ◽  
Octadecanoic Acid ◽  
Hibiscus Rosa Sinensis ◽  
Vero Cell Line ◽  
Phytochemical Compounds

A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract.

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