Increased cellular resistance to oxidative stress by expression of cyanobacterium catalase-peroxidase in animal cells

ABSTRACT All aerobic organisms have mechanisms that protect against oxidative compounds. Catalase, peroxidase, superoxide dismutase, glutathione, and thioredoxin are widely distributed in many taxa and constitute elements of a nearly ubiquitous antioxidant metabolic strategy. Interestingly, the regulatory mechanisms that control these elements are rather different depending on the nature of the oxidative stress and the organism. Catalase is well documented to play an important role in protecting cells from oxidative stress. In particular, pathogenic bacteria seem to use this enzyme as a defensive tool against attack by the host. To investigate the significance of catalase in hostile environments, we made catalase deletion mutations in two different B. abortus strains and used two-dimensional gel analysis, survival tests, and adaptation experiments to explore the behavior and role of catalase under several oxidative stress conditions. These studies show that B. abortus strains that do not express catalase activity exhibit increased sensitivity to hydrogen peroxide. We also demonstrate that catalase expression is regulated in this species, and that preexposure to a sublethal concentration of hydrogen peroxide allows B. abortus to adapt so as to survive subsequent exposure to higher concentrations of hydrogen peroxide.

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A bifunctional catalase-peroxidase,MakatG1, contributes to virulence ofMetarhizium acridumby overcoming oxidative stress on the host insect cuticle

Environmental Microbiology ◽

10.1111/1462-2920.13932 ◽

2017 ◽

Vol 19 (10) ◽

pp. 4365-4378 ◽

Cited By ~ 12

Author(s):

Guohong Li ◽

Anni Fan ◽

Guoxiong Peng ◽

Nemat O. Keyhani ◽

Jiankang Xin ◽

...

Keyword(s):

Oxidative Stress ◽

Insect Cuticle ◽

Host Insect ◽

Catalase Peroxidase

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The cellular resistance against oxidative stress (H2O2) is independent of neutral trehalase (Ntc1p) activity in Candida albicans

FEMS Yeast Research ◽

10.1111/j.1567-1364.2006.00069.x ◽

2006 ◽

Vol 6 (2) ◽

pp. 319-319 ◽

Cited By ~ 3

Author(s):

Yolanda Pendreño ◽

Pilar González-Párraga ◽

Sergio Conesa ◽

María Martínez-Esparza ◽

Ana Aguinaga ◽

...

Keyword(s):

Oxidative Stress ◽

Candida Albicans ◽

Cellular Resistance ◽

Neutral Trehalase

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Correction: Tualang Honey Improves Human Corneal Epithelial Progenitor Cell Migration and Cellular Resistance to Oxidative Stress In Vitro

PLoS ONE ◽

10.1371/journal.pone.0105233 ◽

2014 ◽

Vol 9 (8) ◽

pp. e105233

Author(s):

Keyword(s):

Oxidative Stress ◽

Cell Migration ◽

Progenitor Cell ◽

Cellular Resistance ◽

Corneal Epithelial ◽

Tualang Honey

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Evidence that disruption of cytosolic calcium is critically important in oxidative plant stress

Proceedings of the Royal Society of Edinburgh Section B Biological Sciences ◽

10.1017/s0269727000014202 ◽

1994 ◽

Vol 102 ◽

pp. 261-264 ◽

Cited By ~ 1

Author(s):

J. Lock ◽

A. H. Price

Keyword(s):

Oxidative Stress ◽

Calcium Homeostasis ◽

Cellular Response ◽

Animal Cell ◽

Plant Stress ◽

Temporal Distribution ◽

Cytosolic Calcium ◽

Plant Responses ◽

Animal Cells ◽

Transient Elevation

It is no longer doubted that calcium functions as a second messenger in animals and plants. This is only possible because cells maintain cytosolic calcium concentrations many orders of magnitude below that of extracellular or organelle calcium. Environmental stimuli are perceived by receptor proteins which trigger transient elevation of cytosolic calcium using internal or external sources. The spatial and temporal distribution and the magnitude of calcium elevation determines the specific cellular response at the molecular level (Cheek 1991). The fine balance of cytosolic calcium homeostasis in animal cells is highly sensitive to oxidising conditions (Duncan 1991). Elevated cytosolic calcium resulting from oxidative perturbation of calcium homeostasis is believed to be responsible for the subsequent cellular injury and death (Nicotera et al. 1991). Transient stimulation of cytosolic calcium in sublethal oxidative stress may be a mechanism by which oxidative attack is perceived by the animal cell (Nicotera et al. 1991). Our understanding of oxidative stress and plant responses to it would be greatly advanced if it can be shown that similar processes occur in plant cells. This paper briefly presents the mechanism of oxidative disruption of calcium homeostasis in animal cells and summarises the evidence that the same scenario applies to plants.

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Enzyme activities associated with oxidative stress in Metarhizium anisopliae during germination, mycelial growth, and conidiation and in response to near-UV irradiation

Canadian Journal of Microbiology ◽

10.1139/w03-097 ◽

2004 ◽

Vol 50 (1) ◽

pp. 41-49 ◽

Cited By ~ 33

Author(s):

Charles D Miller ◽

Drauzio Rangel ◽

Gilberto UL Braga ◽

Stephan Flint ◽

Sun-Il Kwon ◽

...

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Glutathione Reductase ◽

Stress Responses ◽

Resistant Strain ◽

Metarhizium Anisopliae ◽

Specific Activity ◽

Major Effect ◽

Solar Irradiation ◽

Catalase Peroxidase

Metarhizium anisopliae isolates have a wide insect host range, but an impediment to their commercial use as a biocontrol agent of above-ground insects is the high susceptibility of spores to the near-UV present in solar irradiation. To understand stress responses in M. anisopliae, we initiated studies of enzymes that protect against oxidative stress in two strains selected because their spores differed in sensitivity to UV-B. Spores of the more near-UV resistant strain in M. anisopliae 324 displayed different isozyme profiles for catalaseperoxidase, glutathione reductase, and superoxide dismutase when compared with the less resistant strain 2575. A transient loss in activity of catalaseperoxidase and glutathione reductase was observed during germination of the spores, whereas the intensity of isozymes displaying superoxide dismutase did not change as the mycelium developed. Isozyme composition for catalaseperoxidases and glutathione reductase in germlings changed with growth phase. UV-B exposure from lamps reduced the activity of isozymes displaying catalaseperoxidase and glutathione reductase activities in 2575 more than in 324. The major effect of solar UV-A plus UV-B also was a reduction in catalaseperoxidases isozyme level, a finding confirmed by measurement of catalase specific activity. Impaired growth of M. anisopliae after near-UV exposure may be related to reduced abilities to handle oxidative stress.Key words: catalaseperoxidase, germination, glutathione reductase, Metarhizium anisopliae, near-UV, protein oxidation, superoxide dismutase.

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