scholarly journals Tyrosine kinase(s) regulate apoptosis and bcl-2 expression in a growth factor-dependent cell line.

1993 ◽  
Vol 268 (30) ◽  
pp. 22733-22736
Author(s):  
H Otani ◽  
M Erdos ◽  
W.J. Leonard
Keyword(s):  
Growth Factor ◽  
Tyrosine Kinase ◽  
Cell Line ◽  
Dependent Cell

scholarly journals The extracytoplasmic domain of the erythropoietin receptor forms a monomeric complex with erythropoietin

Blood ◽  
1993 ◽  
Vol 82 (6) ◽  
pp. 1713-1719 ◽  
Author(s):  
MG Yet ◽  
SS Jones
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Gel Filtration ◽  
Chinese Hamster ◽  
Erythropoietin Receptor ◽  
Apparent Dissociation Constant ◽  
Amino Terminal ◽  
Truncated Receptor ◽  
Monomeric Complex ◽  
Dependent Cell

Abstract We have generated a truncated form of the erythropoietin receptor (EPO- R), the extracytoplasmic ligand-binding domain, that is secreted from a transfected Chinese hamster ovary (CHO) cell line. The truncated receptor is readily purified from CHO conditioned media as a 33-Kd glycosylated protein, which is converted to a 25-Kd species upon treatment with protein N-glycan glycosidase F. Cross-linking of radioiodinated EPO to the secreted receptor yielded a complex of 72 Kd. Also, the growth of the EPO-dependent cell line, FDCPE, was inhibited in a dose-responsive manner by the truncated receptor. The complex of the secreted receptor and EPO was isolated by gel filtration and shown to be a one-to-one complex of the receptor and growth factor by quantitative amino terminal sequencing. Finally, analysis of the interaction of the receptor and growth factor by gel filtration indicated an apparent dissociation constant of 1.1 nmol/L for the truncated receptor.

scholarly journals CLM3, a Multitarget Tyrosine Kinase Inhibitor With Antiangiogenic Properties, Is Active Against Primary Anaplastic Thyroid Cancer In Vitro and In Vivo

2014 ◽  
Vol 99 (4) ◽  
pp. E572-E581 ◽  
Author(s):  
Alessandro Antonelli ◽  
Guido Bocci ◽  
Poupak Fallahi ◽  
Concettina La Motta ◽  
Silvia Martina Ferrari ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Thyroid Cancer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Tyrosine Kinase ◽  
Cell Line ◽  
Growth Factor Receptor ◽  
Anaplastic Thyroid Cancer ◽  
Antiangiogenic Activity ◽  
Epidermal Growth

Context and Objective: We have studied the antitumor activity of a pyrazolo[3,4-d]pyrimidine compound (CLM3) proposed for a multiple signal transduction inhibition [including the RET tyrosine kinase, epidermal growth factor receptor, and vascular endothelial growth factor (VEGF) receptor and with antiangiogenic activity] in primary anaplastic thyroid cancer (ATC) cells, in the human cell line 8305C (undifferentiated thyroid cancer), and in an ATC-cell line (AF). Design and Main Outcome Measures: CLM3 was tested in primary ATC cells at the concentrations of 5, 10, 30, and 50 μM; in 8305C cells, in AF cells, at 1, 5, 10, 30, 50, or 100 μM; and in AF cells in CD nu/nu mice. Results: CLM3 significantly inhibited the proliferation of 8305C and AF cells, also inducing apoptosis. A significant reduction of proliferation with CLM3 in ATC cells (P < .01, ANOVA) was shown. CLM3 increased the percentage of apoptotic ATC cells dose dependently (P < .001, ANOVA) and inhibited migration (P < .01) and invasion (P < .001). The AF cell line was injected sc in CD nu/nu mice, and tumor masses became detectable 15 days later. CLM3 (50 mg/kg per die) significantly inhibited tumor growth (starting 16 d after the beginning of treatment). CLM3 significantly decreased the VEGF-A expression and microvessel density in AF tumor tissues. Furthermore, CLM3 inhibited epidermal growth factor receptor, AKT, and ERK1/2 phosphorylation and down-regulated cyclin D1 in 8305C and AF cells. Conclusions: The antitumor and antiangiogenic activity of a pyrazolo[3,4-d]pyrimidine compound (CLM3) is very promising in anaplastic thyroid cancer, opening the way to a future clinical evaluation.

Expression of bcr-abl abrogates factor-dependent growth of human hematopoietic M07E cells by an autocrine mechanism

Blood ◽  
1994 ◽  
Vol 83 (6) ◽  
pp. 1575-1585 ◽  
Author(s):  
C Sirard ◽  
P Laneuville ◽  
JE Dick
Keyword(s):  
Growth Factor ◽  
Tyrosine Kinase ◽  
Neutralizing Antibodies ◽  
Pleiotropic Effect ◽  
Biologically Active ◽  
Gm Csf ◽  
Dependent Cell ◽  
Macrophage Colony ◽  
Dependent Growth ◽  
Autocrine Mechanism

The introduction of a retrovirus vector expressing p210bcr-abl (P210) into the human factor-dependent cell line M07E resulted in the rapid outgrowth of factor-independent cells. Early after infection, four factor-independent clones were isolated and analyzed in greater detail along with mass populations obtained from separate infections. High levels of P210 tyrosine kinase activity were measured in the factor- independent cells. The mass populations and three of the four clones remained responsive to exogenous growth factors. Concentrated conditioned media isolated from the factor-independent populations and from all clones contained biologically active granulocyte-macrophage colony-stimulating factor (GM-CSF); interleukin-3 (IL-3) was detected at low levels in the mass population and in two of the clones. Neutralizing antibodies to IL-3, GM-CSF, and mast cell growth factor inhibited proliferation of the factor responsive clones by 60% to 90%. These results indicate that the growth autonomy of the P210-expressing M07E cells was acquired via an autocrine mechanism. In addition to factor-independent growth, P210-expressing M07E cells readily acquired a more mature megakaryocytic phenotype compared with control M07E cells. These data provide experimental evidence that expression of P210 tyrosine kinase in human hematopoietic cells induced growth factor secretion resulting in a pleiotropic effect on growth factor dependence and differentiation.

Altered regulation of Src tyrosine kinase by transforming growth factor ?1 in a human hepatoma cell line

Hepatology ◽  
1998 ◽  
Vol 28 (3) ◽  
pp. 796-804 ◽  
Author(s):  
Kazuto Fukuda ◽  
Sumio Kawata ◽  
Shinji Tamura ◽  
Yukihiko Matsuda ◽  
Yoshiaki Inui ◽  
...  
Keyword(s):  
Growth Factor ◽  
Tyrosine Kinase ◽  
Cell Line ◽  
Transforming Growth Factor ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Src Tyrosine Kinase ◽  
Altered Regulation

AP-1/C-jun and C-myc Regulation During Megakaryocytic Differentiation of a Human Bi-potential Growth-factor-dependent Cell Line

Platelets ◽  
1995 ◽  
Vol 6 (1) ◽  
pp. 24-30
Author(s):  
H. G. Derigs ◽  
D. A. Morgan ◽  
R. Hoffman ◽  
S. L. Litz ◽  
E. F. Srour ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Potential Growth ◽  
Megakaryocytic Differentiation ◽  
Dependent Cell

Insulin-like growth factor-I (IGF-I) stimulates tyrosine kinase activity in purified receptors from a rat liver cell line

1984 ◽  
Vol 119 (1) ◽  
pp. 6-13 ◽  
Author(s):  
Yehiel Zick ◽  
Norio Sasaki ◽  
Robert W. Rees-Jones ◽  
George Grunberger ◽  
S.Peter Nissley ◽  
...  
Keyword(s):  
Growth Factor ◽  
Tyrosine Kinase ◽  
Cell Line ◽  
Rat Liver ◽  
Liver Cell ◽  
Kinase Activity ◽  
Tyrosine Kinase Activity ◽  
Factor I ◽  
Liver Cell Line ◽  
Igf I

scholarly journals Apoptosis is regulated by the rate of glucose transport in an interleukin 3 dependent cell line.

1994 ◽  
Vol 180 (3) ◽  
pp. 917-923 ◽  
Author(s):  
O Kan ◽  
S A Baldwin ◽  
A D Whetton
Keyword(s):  
Growth Factor ◽  
Tyrosine Kinase ◽  
Glucose Uptake ◽  
Cell Line ◽  
Glucose Transport ◽  
Alternative Energy ◽  
Protein Tyrosine Kinase ◽  
Permissive Temperature ◽  
Interleukin 3 ◽  
Abl Tyrosine Kinase

In the absence of a survival stimulus, the interleukin 3 (IL-3)-dependent IC.DP cell line undergoes a process termed programmed cell death or apoptosis. Survival can be induced by IL-3, which can also stimulate proliferation of IC.DP cells. IC.DP cells have been stably transfected with the p160v-abl protein tyrosine kinase, activation of the kinase at the permissive temperature permits cell survival in the absence of IL-3 by suppression of apoptosis, although the growth factor is still required for proliferation. Both IL-3 and activation of the v-ABL tyrosine kinase stimulated glucose transport, which may in part be due to a translocation of transporters to the cell surface. Inhibition of glucose uptake markedly increased the rate of apoptosis in these cells, an effect that could be reversed by the provision of alternative energy sources such as glutamine. Growth factor- or oncogene-mediated increases in glucose uptake may therefore represent an important regulatory point in the suppression of apoptosis.

scholarly journals Synthesis, Evaluation for Cytotoxicity and Molecular Docking Studies of Benzo[c]furan-Chalcones for Potential to Inhibit Tubulin Polymerization and/or EGFR-Tyrosine Kinase Phosphorylation

2018 ◽  
Vol 19 (9) ◽  
pp. 2552 ◽  
Author(s):  
Malose Mphahlele ◽  
Marole Maluleka ◽  
Nishal Parbhoo ◽  
Sibusiso Malindisa
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Molecular Docking ◽  
Growth Factor ◽  
Tyrosine Kinase ◽  
Cell Line ◽  
Receptor Tyrosine Kinase ◽  
Growth Factor Receptor ◽  
Tubulin Polymerization ◽  
Epidermal Growth ◽  
Mcf 7

A series of 2-arylbenzo[c]furan-chalcone hybrids 3a–y have been synthesized and evaluated for antiproliferative effects against the human breast cancer (MCF-7) cell line and for its potential to induce apoptosis and also to inhibit tubulin polymerization and/or epidermal growth factor receptor-tyrosine kinase (EGFR-TK) phosphorylation. Most of these compounds exhibited moderate to significant antigrowth effects in vitro against the MCF-7 cell line when compared to the reference standard actinomycin D. The capabilities of the most cytotoxic benzofuran-chalcone hybrids 3b and 3i, to induce apoptosis, have been evaluated by Annexin V-Cy3 SYTOX staining and caspase-3 activation. The experimental and molecular docking results suggest that the title compounds have the potential to exhibit inhibitory effects against tubulin polymerization and epidermal growth factor receptor tyrosine kinase (EGFR-TK) phosphorylation. The modeled structures of representative compounds displayed hydrophobic interactions as well as hydrogen and/or halogen bonding with the protein residues. These interactions are probably responsible for the observed increased binding affinity for the two receptors and their significant antigrowth effect against the MCF-7 cell line.

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