scholarly journals THE EXTRACTION OF FOLIC (PTEROYLGLUTAMIC) ACID CONJUGATE FROM YEAST

1949 ◽  
Vol 179 (3) ◽  
pp. 1075-1078
Author(s):  
Richard D. Greene
Keyword(s):  
Pteroylglutamic Acid ◽  
Acid Conjugate

scholarly journals Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount

Analytica ◽  
2021 ◽  
Vol 2 (3) ◽  
pp. 66-75
Author(s):  
Toshiki Horikoshi ◽  
Chihiro Kitaoka ◽  
Yosuke Fujii ◽  
Takashi Asano ◽  
Jiawei Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Glucuronic Acid ◽  
Laser Desorption ◽  
The Body ◽  
Time Dependent ◽  
Laser Desorption Ionization ◽  
Fingerprint Analysis ◽  
Desorption Ionization ◽  
Acid Conjugate ◽  
Over Time

The ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the fingerprint taken 4 h after AAP ingestion, not only AAP but also the glucuronic acid conjugate of AAP (GAAP), caffeine (Caf), ethenzamide (Eth), salicylamide (Sala; a metabolite of Eth), and urea were detected. Fingerprints were collected over time to determine how the amounts of AAP and its metabolite changed with time, and the time dependence of the peak intensities of protonated AAP and GAAP was measured. It was found that the increase of [GAAP+H]+ peak started later than that of [AAP+H]+ peak, reflecting the metabolism of AAP. Both AAP and GAAP reached maximum concentrations approximately 3 h after ingestion, and were excreted from the body with a half-life of approximately 3.3 h. In addition, fingerprint preservation was confirmed by optical microscopy, and fingerprint shape was retained even after laser irradiation of the fingerprint. Our method may be used in fingerprint analysis.

scholarly journals Molecular Targeting of VEGF with a Suramin Fragment–DOCA Conjugate by Mimicking the Action of Low Molecular Weight Heparins

Biomolecules ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 46
Author(s):  
Jooho Park ◽  
Tae-Bong Kang ◽  
Ji-Hong Lim ◽  
Hyung-Sik Won
Keyword(s):  
Therapeutic Potential ◽  
Umbilical Vein ◽  
Pharmaceutical Research ◽  
Molecular Targeting ◽  
Drug Candidate ◽  
Heparin Binding ◽  
Vegf Inhibitor ◽  
Acid Conjugate ◽  
Pathogenic Action ◽  
Umbilical Vein Endothelial Cells

Molecular targeting of growth factors has shown great therapeutic potential in pharmaceutical research due to their roles in pathological conditions. In the present study, we developed a novel suramin fragment and deoxycholic acid conjugate (SFD) that exhibited the potential to bind to the heparin-binding site (HBD) of vascular endothelial growth factor (VEGF) and to inhibit its pathogenic action for the first time. Notably, SFD was optimally designed for binding to the HBD of VEGF using the naphthalenetrisulfonate group, allowing to observe its excellent binding efficacy in a surface plasmon resonance (SPR) study, showing remarkable binding affinity (KD = 3.8 nM) as a small molecule inhibitor. In the tubular formation assay, it was observed that SFD could bind to HBD and exhibit antiangiogenic efficacy by inhibiting VEGF, such as heparins. The cellular treatment of SFD resulted in VEGF-inhibitory effects in human umbilical vein endothelial cells (HUVECs). Therefore, we propose that SFD can be employed as a novel drug candidate to inhibit the pathophysiological action of VEGF in diseases. Consequently, SFD, which has a molecular structure optimized for binding to HBD, is put forward as a new chemical VEGF inhibitor.

scholarly journals A CHEMICAL METHOD FOR THE DETERMINATION OF PTEROYLGLUTAMIC ACID AND RELATED COMPOUNDS

1947 ◽  
Vol 168 (2) ◽  
pp. 705-710
Author(s):  
B.L. Hutchings ◽  
E.L.R. Stokstad ◽  
J.H. Boothe ◽  
J.H. Mowat ◽  
C.W. Waller ◽  
...  
Keyword(s):  
Chemical Method ◽  
Pteroylglutamic Acid ◽  
Related Compounds
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