scholarly journals Molecular cloning and expression of a Leishmania major gene encoding a single-stranded DNA-binding protein containing nine “CCHC” zinc finger motifs

1993 ◽  
Vol 268 (19) ◽  
pp. 13994-14002
Author(s):  
J.R. Webb ◽  
W.R. McMaster
Keyword(s):  
Dna Binding ◽  
Molecular Cloning ◽  
Zinc Finger ◽  
Leishmania Major ◽  
Major Gene ◽  
Dna Binding Protein ◽  
Cloning And Expression ◽  
Gene Encoding ◽  
Zinc Finger Motifs ◽  
Cchc Zinc Finger

scholarly journals Evi-1, a murine zinc finger proto-oncogene, encodes a sequence-specific DNA-binding protein.

1991 ◽  
Vol 11 (5) ◽  
pp. 2665-2674 ◽  
Author(s):  
A S Perkins ◽  
R Fishel ◽  
N A Jenkins ◽  
N G Copeland
Keyword(s):  
Dna Binding ◽  
Binding Site ◽  
Zinc Finger ◽  
Sequence Data ◽  
Binding Protein ◽  
Consensus Sequence ◽  
Dna Binding Protein ◽  
Polymerase Chain Reaction Method ◽  
Nucleotide Sequence Data ◽  
Carboxy Terminal

Evi-1 was originally identified as a common site of viral integration in murine myeloid tumors. Evi-1 encodes a 120-kDa polypeptide containing 10 zinc finger motifs located in two domains 380 amino acids apart and an acidic domain located carboxy terminal to the second set of zinc fingers. These features suggest that Evi-1 is a site-specific DNA-binding protein involved in the regulation of RNA transcription. We have purified Evi-1 protein from E. coli and have employed a gel shift-polymerase chain reaction method using random oligonucleotides to identify a high-affinity binding site for Evi-1. The consensus sequence for this binding site is TGACAAGATAA. Evi-1 protein specifically protects this motif from DNase I digestion. By searching the nucleotide sequence data bases, we have found this binding site both in sequences 5' to genes in putative or known regulatory regions and within intron sequences.

Molecular analysis of the Escherichia coli has gene encoding a DNA-binding protein, which preferentially recognizes curved DNA sequences

1991 ◽  
Vol 230 (1-2) ◽  
pp. 332-336 ◽  
Author(s):  
Hisami Yamada ◽  
Takayuki Yoshida ◽  
Ken-ichi Tanaka ◽  
Chihiro Sasakawa ◽  
Takeshi Mizuno
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Molecular Analysis ◽  
Dna Sequences ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Gene Encoding ◽  
Curved Dna
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