Sodium dodecylsulfate capillary gel electrophoretic measurement of the concentration ratios of albumin and α2-macroglobulin in cerebrospinal fluid and serum of patients with neurological disorders

Background: Cerebrospinal fluid (CSF) free light chains (FLCs) can be an alternative assay to oligoclonal bands (OCBs) in inflammatory neurological disorders, but threshold has no consensus. Objective: To assess the diagnostic accuracy of CSF FLCs in multiple sclerosis (MS) and other neurological diseases. Methods: A total of 406 patients from five Italian centers. FLCs were measured in CSF and serum using Freelite MX assays on Optilite. Results: A total of 171 patients were diagnosed as MS, 154 non-inflammatory neurological diseases, 48 inflammatory central nervous system (CNS) diseases, and 33 peripheral neurological diseases. Both kFLC and λFLC indices were significantly higher in patients with MS compared to other groups ( p < 0.0001). The kFLC index ⩾ 6.4 is comparable to OCB for MS diagnosis (area under the receiver operating characteristic curve (AUC) = 0.876; sensitivity 83.6% vs 84.2%; specificity 88.5% vs 90.6%). λFLC index ⩾ 5 showed an AUC of 0.616, sensitivity of 33.3% and specificity of 90.6%. In all, 12/27 (44.4%) MS patients with negative OCB had kFLC index ⩾ 6.4. Interestingly, 37.5% of 24 patients with a single CSF IgG band showed high kFLC index and 12.5% positive λFLC index. Conclusion: Our findings support the diagnostic utility of FLC indices in MS and other CNS inflammatory disorders, suggesting a combined use of FLC and OCB to help clinicians with complementary information.

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Central and Peripheral Myelin Basic Protein in Cerebrospinal Fluid of Multiple Sclerosis and Other Neurological Disorders

Progress in Multiple Sclerosis Research ◽

10.1007/978-3-642-67554-6_30 ◽

1980 ◽

pp. 170-173

Author(s):

K. Hempel ◽

D. Dommasch ◽

W. Brors

Keyword(s):

Multiple Sclerosis ◽

Cerebrospinal Fluid ◽

Myelin Basic Protein ◽

Neurological Disorders ◽

Basic Protein

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A New Approach for Early Assessment of the Epileptogenic Potential of Quinolones

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.42.10.2756 ◽

1998 ◽

Vol 42 (10) ◽

pp. 2756-2758 ◽

Cited By ~ 3

Author(s):

Annie Delon ◽

Claudine Pariat ◽

Philippe Courtois ◽

Serge Bouquet ◽

William Couet

Keyword(s):

Cerebrospinal Fluid ◽

Animal Species ◽

Early Assessment ◽

New Approach ◽

Drug Concentrations ◽

Quinolone Antibiotics ◽

Concentration Ratios

ABSTRACT The epileptogenic potential of pefloxacin and norfloxacin, two quinolone antibiotics, was investigated in vivo in three different animal species by measuring drug concentrations in cerebrospinal fluid (CSF), which is part of the biophase, at the onset of convulsions. Interestingly, the pefloxacin-to-norfloxacin concentration ratios in CSF were virtually constant across the species (7.0, 6.6, and 6.0 in mice, rats, and rabbits, respectively), suggesting that this approach could be used to predict the relative epileptogenic potential of quinolones in humans.

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HPLC method for measurement of purine nucleotide degradation products in cerebrospinal fluid

Clinical Chemistry ◽

10.1093/clinchem/42.5.756 ◽

1996 ◽

Vol 42 (5) ◽

pp. 756-760 ◽

Cited By ~ 17

Author(s):

L Kuracka ◽

T Kalnovicová ◽

B Líska ◽

P Turcáni

Keyword(s):

Cerebrospinal Fluid ◽

Uric Acid ◽

Neurological Disorders ◽

Limit Of Detection ◽

Degradation Products ◽

Potassium Phosphate ◽

Hplc Method ◽

Potassium Phosphate Buffer ◽

Nucleotide Degradation

Abstract We describe a convenient method for the separation and quantification of xanthine, hypoxanthine, and uric acid in 20 microL of cerebrospinal fluid (CSF) with use of HPLC and ultraviolet detection. The analysis is performed on a Sepharon SGX C18 column and the elution system consists of potassium phosphate buffer, pH 5.1, with 20 mL/L methanol. The lower limit of detection was 4 pmol for hypoxanthine and xanthine and 6 pmol for uric acid. Analytical recoveries of purine metabolites ranged from 98.6% to 102.9%. The intra- and interassay CVs were <3%. The applicability of the method is illustrated with the determination of micromolar concentrations of xanthine, hypoxanthine, and uric acid in CSF samples obtained from 113 patients with various neurological disorders.

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Cerebrospinal Fluid Proteomics For Identification Of α2-Macroglobulin As A Potential Biomarker To Monitor Pharmacological Therapeutic Efficacy In Dopamine Dictated Disease States Of Parkinson’s Disease And Schizophrenia

Neuropsychiatric Disease and Treatment ◽

10.2147/ndt.s214217 ◽

2019 ◽

Vol Volume 15 ◽

pp. 2853-2867 ◽

Cited By ~ 1

Author(s):

Ashish Kumar Gupta ◽

Ruchika Pokhriyal ◽

Mohd Imran Khan ◽

Domada Ratna Kumar ◽

Rishab Gupta ◽

...

Keyword(s):

Parkinson’S Disease ◽

Parkinson's Disease ◽

Cerebrospinal Fluid ◽

Therapeutic Efficacy ◽

Disease States ◽

Α2 Macroglobulin ◽

Potential Biomarker

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Time-resolved immunofluorometric assay of complement C3: application to cerebrospinal fluid

Clinical Chemistry ◽

10.1093/clinchem/39.2.309 ◽

1993 ◽

Vol 39 (2) ◽

pp. 309-312 ◽

Cited By ~ 7

Author(s):

O Gaillard ◽

D Meillet ◽

M C Diemert ◽

L Musset ◽

J Delattre ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Neurological Disorders ◽

Biological Fluids ◽

Clinical Applications ◽

Complement C3 ◽

Complement Components ◽

Time Resolved ◽

Sandwich Type ◽

Analytical Range ◽

Immunochemical Methods

Abstract Complement components have a role in various neurological disorders. Complement C3 can be measured by immunochemical methods, but only radioimmunoassays and electroimmunodiffusion assays (EIDs) are sufficiently sensitive to be applied to biological fluids in which the C3 concentration is low, especially cerebrospinal fluid (CSF). We report a sandwich-type time-resolved immunofluorometric assay (TR-IFMA) for C3 in CSF. The linearity (0.7-3650 micrograms/L) and intra- (CV < 4.8%) and inter-assay (CV < 10.9%) precision were satisfactory and the results agreed with those of EID. The assay is extremely sensitive (< 1 microgram/L) and its analytical range is large and well suited to clinical applications. This simple TR-IFMA is thus a nonisotopic alternative to radioimmunoassay for the quantification of complement C3 in CSF.

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