A Chicken Chorioallantoic Membrane Assay for the Evaluation of the Androgen Responsiveness of Prostatic Tissue

1986 ◽  
Vol 135 (6) ◽  
pp. 1312-1318 ◽  
Author(s):  
Jonathan P. Jarow ◽  
Fray F. Marshall ◽  
John T. Isaacs
Keyword(s):  
Chorioallantoic Membrane ◽  
Prostatic Tissue ◽  
Chicken Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

scholarly journals Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay

2005 ◽  
Vol 93 (3) ◽  
pp. 317-323 ◽  
Author(s):  
Serafim Kiriakidis ◽  
Oliver Högemeier ◽  
Susanne Starcke ◽  
Frank Dombrowski ◽  
Jens Claus Hahne ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cell ◽  
Molecular Mechanisms ◽  
Inflammatory Diseases ◽  
Chorioallantoic Membrane ◽  
Endothelial Cell Proliferation ◽  
Inhibition Of Angiogenesis ◽  
Chicken Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

Anti-angiogenic strategies are emerging as an important tool for the treatment of cancer and inflammatory diseases. In the present investigation we isolated several isoflavones from a tempeh (fermented soyabean) extract. The isolated isoflavones were identified as 5,7,4′-trihydroxyisoflavone (genistein), 7,4′-dihydroxyisoflavone (daidzein), 6,7,4′-trihydroxyisoflavone (factor 2), 7,8,4′-trihydroxyisoflavone (7,8,4′-TriOH) and 5,7,3′,4′-tetrahydroxyisoflavone (orobol). The effects on angiogenesis of these isoflavones were evaluated in the chicken chorioallantoic membrane assay; their capacity to inhibit vascular endothelial growth factor-induced endothelial cell proliferation and expression of the Ets 1 transcription factor, known to be implicated in the regulation of new blood vessel formation, were also investigated. We found that all isoflavones inhibited angiogenesis, albeit with different potencies. Compared with negative controls, which slightly inhibited in vivo angiogenesis by 6·30 %, genistein reduced angiogensis by 75·09 %, followed by orobol (67·96 %), factor 2 (56·77 %), daidzein (48·98 %) and 7,8,4′-TriOH (24·42 %). These compounds also inhibited endothelial cell proliferation, with orobol causing the greatest inhibition at lower concentrations. The isoflavones also inhibited Ets 1 expression, providing some insight into the molecular mechanisms of their action. Furthermore, the chemical structure of the different isoflavones suggests a structure–activity relationship. Our present findings suggest that the new isoflavones might be added to the list of low molecular mass therapeutic agents for the inhibition of angiogenesis.

scholarly journals The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2613
Author(s):  
Aoi Komatsu ◽  
Kotaro Matsumoto ◽  
Yuki Yoshimatsu ◽  
Yooksil Sin ◽  
Arisa Kubota ◽  
...  
Keyword(s):  
Second Generation ◽  
Fusion Gene ◽  
Chorioallantoic Membrane ◽  
Tumor Formation ◽  
Cancer Type ◽  
Membrane Expression ◽  
Chicken Chorioallantoic Membrane ◽  
Cam Model ◽  
Chorioallantoic Membrane Assay ◽  
Potential Use

(1) Background: CIC-DUX4 sarcoma is a rare mesenchymal small round cell tumor which belongs to rare cancers that occupy a significant percentage of cancer cases as a whole, despite each being rare. Importantly, each rare cancer type has different features, and thus there is a need to develop a model that mimics the features of each of these cancers. We evaluated the idea that the chicken chorioallantoic membrane assay (CAM), a convenient and versatile animal model, can be established for the CIC-DUX4 sarcoma. (2) Methods: Patient-derived cell lines of CIC-DUX4 were applied. These cells were transplanted onto the CAM membrane and tumor formation was examined by H&E staining, immunohistochemistry and Western blotting. The CAM tumor was transferred onto a fresh CAM and was also used to form organoids. Retention of the fusion gene was examined. (3) Results: H&E staining as well as molecular characterization demonstrated the formation of the CIC-DUX4 tumor on the CAM membrane. Expression of cyclin D2 and ETV4 was identified. The CAM tumor was transferred to a fresh CAM to form the second-generation CAM tumor. In addition, we were successful in forming tumor organoids using the CAM tumor. Retention of the fusion gene CIC-DUX4 in the CAM, second-generation CAM, and in the CAM-derived organoids was confirmed by RT-PCR. (4) Conclusions: The CAM assay provides a promising model for CIC-DUX4 sarcoma.

scholarly journals The Aqueous Soluble Polyphenolic Fraction ofPsidium guajavaLeaves Exhibits Potent Anti-Angiogenesis and Anti-Migration Actions on DU145 Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Chiung-Chi Peng ◽  
Chiung-Huei Peng ◽  
Kuan-Chou Chen ◽  
Chiu-Lan Hsieh ◽  
Robert Y. Peng
Keyword(s):  
Chorioallantoic Membrane ◽  
Psidium Guajava ◽  
Elisa Assay ◽  
Scratch Assay ◽  
Anti Cancer ◽  
Assay Methods ◽  
Du145 Cells ◽  
Chicken Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay ◽  
Wound Scratch Assay

The aqueous extract ofPsidium guajavabudding leaves (PE) bears an extremely high content of polyphenolic and isoflavonoids. Whether it could be used as an anti-tumor chemopreventive in view of anti-angiogenesis and anti-migration, we performed the assay methods including the MTT assay to examine the cell viability; the ELISA assay to test the expressions of VEGF, IL-6 and IL-8; the western blot analysis to detect TIMP-2; the gelatinolytic zymography to follow the expression of MMPs; the wound scratch assay to examine the migration capability; and the chicken chorioallantoic membrane assay to detect the suppressive angiogenesis. Results indicated that the IC50 of PE for DU145 cells was ∼0.57 mg ml−1. In addition, PE effectively inhibited the expressions of VEGF, IL-6 and IL-8 cytokines, and MMP-2 and MMP-9, and simultaneously activated TIMP-2 and suppressed the cell migration and the angiogenesis. Conclusively, PE potentially possesses a strong anti-DU145 effect. Thus, clinically it owns the potential to be used as an effective adjuvant anti-cancer chemopreventive.

scholarly journals Optimization of the chicken chorioallantoic membrane assay as reliable in vivo model for the analysis of osteosarcoma

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0215312 ◽  
Author(s):  
Pierre Kunz ◽  
Astrid Schenker ◽  
Heiner Sähr ◽  
Burkhard Lehner ◽  
Jörg Fellenberg
Keyword(s):  
Chorioallantoic Membrane ◽  
In Vivo Model ◽  
Chicken Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

Inhibition by doxycycline of angiogenesis in the chicken chorioallantoic membrane (CAM)

2005 ◽  
Vol 56 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Mary Richardson ◽  
David Wong ◽  
Samantha Lacroix ◽  
Jolanta Stanisz ◽  
Gurmit Singh
Keyword(s):  
Chorioallantoic Membrane ◽  
Chicken Chorioallantoic Membrane

scholarly journals The Chorioallantoic Membrane Assay for Biomaterial Testing in Tissue Engineering: A Short-TermIn VivoPreclinical Model

2017 ◽  
Vol 23 (12) ◽  
pp. 938-952 ◽  
Author(s):  
Inés Moreno-Jiménez ◽  
Janos M. Kanczler ◽  
Gry Hulsart-Billstrom ◽  
Stefanie Inglis ◽  
Richard O.C. Oreffo
Keyword(s):  
Tissue Engineering ◽  
Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

scholarly journals Angiotropism of Human Melanoma: Studies Involving In Transit and Other Cutaneous Metastases and the Chicken Chorioallantoic Membrane

2006 ◽  
Vol 28 (3) ◽  
pp. 187-193 ◽  
Author(s):  
Claire Lugassy ◽  
Stephen E. Vernon ◽  
Klaus Busam ◽  
Jean A. Engbring ◽  
Danny R. Welch ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Human Melanoma ◽  
Cutaneous Metastases ◽  
In Transit ◽  
Chicken Chorioallantoic Membrane

Role of AKT/PKB signaling in fibroblast growth factor-1 (FGF-1)-induced angiogenesis in the chicken chorioallantoic membrane (CAM)

2004 ◽  
Vol 94 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Reza Forough ◽  
Brian Weylie ◽  
Chirag Patel ◽  
Sandy Ambrus ◽  
Ugra S. Singh ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Chorioallantoic Membrane ◽  
Fibroblast Growth ◽  
Chicken Chorioallantoic Membrane
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