378: 14-3-3 Expression Patterns in the Human Kidney: From Fetal Development to the Adult State to Malignant Transformation

2005 ◽  
Vol 173 (4S) ◽  
pp. 103-103
Author(s):  
Adam G. Baseman ◽  
Andrew J. Kirsch ◽  
Fray F. Marshall ◽  
Haiyen E. Zhau ◽  
Leland W.K. Chung ◽  
...  
Keyword(s):  
Malignant Transformation ◽  
Fetal Development ◽  
Expression Patterns ◽  
Human Kidney

scholarly journals ALDH1 and podoplanin expression patterns predict the risk of malignant transformation in oral leukoplakia

2016 ◽  
Vol 13 (1) ◽  
pp. 321-328 ◽  
Author(s):  
Umma Habiba ◽  
Kyoko Hida ◽  
Tetsuya Kitamura ◽  
Aya Yanagawa Matsuda ◽  
Fumihiro Higashino ◽  
...  
Keyword(s):  
Malignant Transformation ◽  
Expression Patterns ◽  
Oral Leukoplakia ◽  
Podoplanin Expression

Expression patterns of complex glycoconjugates and endogenous lectins during fetal development of the viscerocranium

1999 ◽  
Vol 181 (1) ◽  
pp. 117-121 ◽  
Author(s):  
A. Zschäbitz ◽  
V. Krahn ◽  
W. Schmidt ◽  
H.-J. Gabius ◽  
H. Weiser ◽  
...  
Keyword(s):  
Fetal Development ◽  
Expression Patterns ◽  
Endogenous Lectins

scholarly journals The Expression of Human Placental Genes Related to Carotenoid/retinoid Metabolism and Pathways (P02-005-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Xiaoming Gong ◽  
Lewis Rubin
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Fetal Development ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Gene Set Enrichment Analysis ◽  
Differentially Expressed ◽  
Retinoid Metabolism ◽  
Expression Of Genes ◽  
Β Carotene

Abstract Objectives Carotenoid/retinoids status and metabolism are essential for normal placental and fetal development. Both deficiencies and excess of retinoids and some carotenoids are associated with adverse pregnancy outcomes, such as preeclampsia and preterm birth. A group of important genes involved in regulating carotenoid/retinoid metabolism and maternal to fetal transfer in human placenta. The objective of this study is to analyze (a) the expression of genes critical for regulating carotenoid/retinoid metabolism and maternal-fetal transport in human trophoblasts and (b) placental transcriptional profiles of these pathways in response to carotenoid exposure. Methods Human cytotrophoblasts (CTBs) were isolated from term placentas. CTB RNA was used to analyze the expression of genes involved in carotenoid/retinoid metabolism and pathways by qRT-PCT. First trimester-like trophoblasts (HTR-8/SVneo) were treated with either β-carotene or lycopene. RNAs were isolated and gene expression were analyzed by DNA microarrays. Results Human CTBs express retinoid metabolism and pathways-related genes, including Stra6, Lrat, Rdh5, Rdh10, Aldh1a1, Aldh1a2, Aldh1a3, Aldh8a1, Cyp26a1, and Cyp26b1, but not carotenoid metabolism genes, BCO1 and BCO2. Microarray analysis of placental gene expression profile revealed a total of 872 and 756 differentially expressed genes, respectively, compared to the control. Gene set enrichment analysis and functional annotation clustering was performed to characterize the genes differentially expressed in either β-carotene or lycopene-treated HTR-8/SVneo cells. Many known retinoid metabolism related genes and genes involved in regulation of retinoid signaling were found, and the expression profiles of these genes were markedly different in response to β-carotene treatments. Finally, the qRT-PCR and microarray analysis results showed similar gene expression patterns of carotenoid/retinoid metabolism and pathways. Conclusions These findings suggest that placental expression of genes involved in retinoid metabolism and transport in trophoblasts is critical for regulating retinoid homeostasis during placental and fetal development. Carotenoid exposure in early placental development, significantly modify the placenta gene expression related to retinoid pathways and maternal to fetal transfer. Funding Sources NIH HD421174.

scholarly journals Small proteoglycans of normal adult human kidney: Distinct expression patterns of decorin, biglycan, fibromodulin, and lumican

2000 ◽  
Vol 58 (4) ◽  
pp. 1557-1568 ◽  
Author(s):  
Liliana Schaefer ◽  
Hermann-Josef Gröne ◽  
Igor Raslik ◽  
Horst Robenek ◽  
Jana Ugorcakova ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Human Kidney ◽  
Normal Adult ◽  
Adult Human

Gene expression patterns in melanocytic cells: candidate markers for early stage and malignant transformation

2001 ◽  
Vol 196 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Clifton B. Meije ◽  
Theodorus B. M. Hakvoort ◽  
Guido W. M. Swart ◽  
Wiete Westerhof ◽  
Wouter H. Lamers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Malignant Transformation ◽  
Early Stage ◽  
Expression Patterns ◽  
Gene Expression Patterns

rBAT-b0,+AT heterodimer is the main apical reabsorption system for cystine in the kidney

2002 ◽  
Vol 283 (3) ◽  
pp. F540-F548 ◽  
Author(s):  
Esperanza Fernández ◽  
Montserrat Carrascal ◽  
Ferran Rousaud ◽  
Joaquín Abián ◽  
Antonio Zorzano ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Expression Patterns ◽  
Human Kidney ◽  
Mass Spectrometry Analysis ◽  
Type I ◽  
Spectrometry Analysis ◽  
Sds Page ◽  
Heterologous Cell ◽  
Dibasic Amino Acids

Mutations in the rBAT and b0,+AT genes cause type I and non-type I cystinuria, respectively. The disulfide-linked rBAT-b0,+AT heterodimer mediates high-affinity transport of cystine and dibasic amino acids (b0,+-like activity) in heterologous cell systems. However, the significance of this heterodimer for cystine reabsorption is unknown, as direct evidence for such a complex in vivo is lacking and the expression patterns of rBAT and b0,+AT along the proximal tubule are opposite. We addressed this issue by biochemical means. Western blot analysis of mouse and human kidney brush-border membranes showed that rBAT and b0,+AT were solely expressed as heterodimers of identical size and that both proteins coprecipitated. Moreover, quantitative immunopurification of b0,+AT followed by SDS-PAGE and mass spectrometry analysis established that b0,+AT heterodimerizes exclusively with rBAT. Together with cystine reabsorption data, our results demonstrate that a decreasing expression gradient of heterodimeric rBAT-b0,+AT along the proximal tubule is responsible for virtually all apical cystine reabsorption. As a corollary of the above, there should be an excess of rBAT expression over that of b0,+AT protein in the kidney. Indeed, complete immunodepletion of b0,+AT did not coprecipitate >20–30% of rBAT. Therefore, another rBAT-associated subunit may be present in latter parts of the proximal tubule.

Coordinated regulation of c-Myc and Max in rat liver development

2006 ◽  
Vol 290 (1) ◽  
pp. G145-G155 ◽  
Author(s):  
Jennifer A. Sanders ◽  
Philip A. Gruppuso
Keyword(s):  
Liver Regeneration ◽  
Fetal Development ◽  
Potential Role ◽  
Fetal Liver ◽  
Expression Patterns ◽  
Hepatocyte Proliferation ◽  
Liver Development ◽  
Adult Liver ◽  
Expression Of Genes ◽  
Growth Metabolism

The processes of liver development and regeneration involve regulation of a key network of transcription factors, the c -myc/ max/ mad network. This network regulates the expression of genes involved in hepatocyte proliferation, growth, metabolism, and differentiation. In previous studies on the expression and localization of c-Myc in the fetal and adult liver, we made the unexpected observation that c-Myc content was similar in the two. However, c-Myc was localized predominantly to the nucleolus in the adult liver. On the basis of this finding, we went on to characterize the expression patterns of the other members of the network, max and mad, comparing their regulation during late fetal development with the proliferation of mature hepatocytes that is seen in liver regeneration. We found that Max content, rather than being constitutive, as predicted by other studies, was elevated in the fetal liver compared with the adult liver. Its content correlated with hepatocyte proliferation during the perinatal transition. In contrast, mad4 expression was decreased in the fetal liver compared with the adult liver. Nucleolar localization of c-Myc coincided with changes in Max content. To explore this relationship, we overexpressed Max in cultured adult hepatocytes. High levels of Max resulted in a shift in c-Myc localization from nucleolar to diffuse nuclear. In contrast, liver regeneration was associated with an increase in c-Myc content but no change in Max content. We conclude that the regulation of Max content during liver development and its potential role in determining c-Myc localization are means by which Max may control the biological activity of the c-Myc/Max/Mad network during liver development.

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