Expression and function of cyclooxygenase-2 in mesothelial cells during late phase of rat carrageenin-induced pleurisy

Abstract Background The capacity of the liver to restore its architecture and function assures good prognoses of patients who suffer serious hepatic injury or cancer resection. In our study, we found that the P53/miR-34a/SIRT1 positive feedback loop has a remarkable negative regulatory effect, which is related to the termination of liver regeneration. Here, we described how P53/miR-34a/SIRT1 positive feedback loop controls liver regeneration and its possible relationship with liver cancer.Method We performed partial hepatectomy (PH) in mice transfected with adenovirus (Ade) overexpressing P53 and adenovirus-associated virus (AAV) knock-downing miR-34a. LR was analyzed by liver weight/body weight, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and cell proliferation, and the related cellular signals were investigated. Bile acid (BA) levels during LR were analyzed by metabolomics of bile acids. Results We found that the P53/miR-34a/SIRT1 positive feedback loop was activated in the late phase of LR. Overexpression of P53 terminated LR early and enhanced P53/miR-34a/SIRT1 positive feedback loop expression and its proapoptotic effect. Mice from the Ade-P53 group showed smaller livers and higher levels of serum ALT and AST than control mice. While knock-down of miR-34a abolished P53/miR-34a/SIRT1 positive feedback loop during LR. Mice from anti-miR-34a group showed larger livers and lower levels of PCNA-positive cells than control mice. T-β-MCA increased gradually during LR and peaked at 7 days after PH. T-β-MCA inhibited cell proliferation and promoted cell apoptosis via facilitating the P53/miR-34a/SIRT1 positive feedback loop during LR by suppressing FXR/SHP. Conclusion The P53/miR-34a/SIRT1 positive feedback loop plays an important role in the termination of LR. Our findings shed light on the molecular and metabolic mechanisms of LR termination and provide a potential therapeutic alternative for treating P53-wild-type HCC patients.

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Mesothelial Cells

Peritoneal Dialysis International ◽

10.1177/089686080702702s19 ◽

2007 ◽

Vol 27 (2_suppl) ◽

pp. 110-115 ◽

Cited By ~ 5

Author(s):

Susan Yung ◽

Chan Tak Mao

Keyword(s):

Mesothelial Cell ◽

In Vitro Models ◽

Mesothelial Cells ◽

Dynamic Membrane ◽

Peritoneal Mesothelial Cells ◽

Immunohistochemical Markers ◽

Vitro Model ◽

And Function

♦ Background The introduction of peritoneal dialysis (PD) as a modality of renal replacement therapy has provoked much interest in the biology of the peritoneal mesothelial cell. Mesothelial cells isolated from omental tissue have immunohistochemical markers that are identical to those of mesothelial stem cells, and omental mesothelial cells can be cultivated in vitro to study changes to their biologic functions in the setting of PD. ♦ Method The present article describes the structure and function of mesothelial cells in the normal peritoneum and details the morphologic changes that occur after the introduction of PD. Furthermore, this article reviews the literature of mesothelial cell culture and the limitations of in vitro studies. ♦ Results The mesothelium is now considered to be a dynamic membrane that plays a pivotal role in the homeostasis of the peritoneal cavity, contributing to the control of fluid and solute transport, inflammation, and wound healing. These functional properties of the mesothelium are compromised in the setting of PD. Cultures of peritoneal mesothelial cells from omental tissue provide a relevant in vitro model that allows researchers to assess specific molecular pathways of disease in a distinct population of cells. Structural and functional attributes of mesothelial cells are discussed in relation to long-term culture, proliferation potential, age of tissue donor, use of human or animal in vitro models, and how the foregoing factors may influence in vitro data. ♦ Conclusions The ability to propagate mesothelial cells in culture has resulted, over the past two decades, in an explosion of mesothelial cell research pertaining to PD and peritoneal disorders. Independent researchers have highlighted the potential use of mesothelial cells as targets for gene therapy or transplantation in the search to provide therapeutic strategies for the preservation of the mesothelium during chemical or bacterial injury.

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A new Era of Personalized Medicine for Cystic Fibrosis – at Last!

Canadian Respiratory Journal ◽

10.1155/2015/921712 ◽

2015 ◽

Vol 22 (5) ◽

pp. 257-260 ◽

Cited By ~ 3

Author(s):

Bradley S Quon ◽

Pearce G Wilcox

Keyword(s):

Cystic Fibrosis ◽

Personalized Medicine ◽

Late Phase ◽

Structure And Function ◽

Clinical Development ◽

New Era ◽

High Throughput Drug Screening ◽

Cftr Protein ◽

And Function ◽

Screening Approaches

The gene responsible for cystic fibrosis (CF) was discovered 25 years ago. This breakthrough has enabled a sophisticated understanding of how various mutations lead to specific alterations in the structure and function of the CF transmembrane regulator (CFTR) protein. Until recently, all therapies in CF were focused on ameliorating the downstream consequences of CFTR dysfunction. High-throughput drug screening approaches have yielded compounds that can modify CFTR structure and function, thus targeting the basic defect in CF. The present article describes theCFTRmutational classes, reviews mutation-specific therapies currently in late-phase clinical development, and highlights research opportunities and challenges with personalized medicine in CF.

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Breast cancer cell cyclooxygenase-2 expression alters extracellular matrix structure and function and numbers of cancer associated fibroblasts

Oncotarget ◽

10.18632/oncotarget.14912 ◽

2017 ◽

Vol 8 (11) ◽

pp. 17981-17994 ◽

Cited By ~ 16

Author(s):

Balaji Krishnamachary ◽

Ioannis Stasinopoulos ◽

Samata Kakkad ◽

Marie-France Penet ◽

Desmond Jacob ◽

...

Keyword(s):

Breast Cancer ◽

Extracellular Matrix ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Structure And Function ◽

Cyclooxygenase 2 ◽

Cancer Associated Fibroblasts ◽

Matrix Structure ◽

And Function

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Role of epicardial mesothelial cells in the modification of phenotype and function of adult rat ventricular myocytes in primary coculture.

Circulation Research ◽

10.1161/01.res.71.1.40 ◽

1992 ◽

Vol 71 (1) ◽

pp. 40-50 ◽

Cited By ~ 70

Author(s):

H Eid ◽

D M Larson ◽

J P Springhorn ◽

M A Attawia ◽

R C Nayak ◽

...

Keyword(s):

Ventricular Myocytes ◽

Mesothelial Cells ◽

Rat Ventricular Myocytes ◽

Adult Rat ◽

And Function

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Buffer-dependent regulation of aquaporin-1 expression and function in human peritoneal mesothelial cells

Pediatric Nephrology ◽

10.1007/s00467-012-2120-1 ◽

2012 ◽

Vol 27 (7) ◽

pp. 1165-1177 ◽

Cited By ~ 15

Author(s):

Yihui Zhai ◽

Jacek Bloch ◽

Meike Hömme ◽

Julia Schaefer ◽

Thilo Hackert ◽

...

Keyword(s):

Mesothelial Cells ◽

Peritoneal Mesothelial Cells ◽

Aquaporin 1 ◽

Human Peritoneal Mesothelial Cells ◽

And Function

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Lamellar Inclusions within Hyperplastic Endoplasmic Reticulum in Benign Mesothelial Cells

Acta Cytologica ◽

10.1159/000508757 ◽

2020 ◽

Vol 64 (6) ◽

pp. 572-576

Author(s):

Simon Haefliger ◽

Deepali Jain ◽

Thomas Menter ◽

Tatjana Vlajnic ◽

Spasenija Savic Prince ◽

...

Keyword(s):

Endoplasmic Reticulum ◽

Daily Practice ◽

Mesothelial Cells ◽

Routine Practice ◽

Consecutive Series ◽

Diagnostic Utility ◽

Tubular Structures ◽

Lamellar Inclusions ◽

Malignant Effusions ◽

And Function

Introduction: In effusion cytology, mesothelial cells can occasionally present with striking intracytoplasmic accumulation of rod- and crystal-like cytoplasmic lamellar inclusions (LIs). Their nature and function are poorly understood, and their diagnostic relevance is unknown. Objective: The aim of this study was to explore the nature of LIs in mesothelial cells and determine their prevalence and diagnostic utility in routine practice. Material and Method: We reviewed a consecutive series of cytological specimens of reactive (n = 102) and malignant effusions (n = 90), respectively. Malignant effusions included malignant mesotheliomas (n = 63) and carcinomas (n = 27). LIs of one effusion were analyzed by electron microscopy (EM). Results: LIs were found exclusively in benign mesothelial cells in 14% (14/102) of reactive and in 4% (1/27) of malignant effusions with carcinomatosis. They were absent in effusions of malignant mesothelioma. EM revealed mainly straight lamellar, less tubular, structures in cisternae of the hyperplasic rough endoplasmic reticulum (rER). Conclusion: Cytoplasmic LIs located within hyperplastic rER can be found in up to 14% of effusions restricted to benign mesothelial cells. They can be used as an indirect morphological clue favoring the diagnosis of benign effusion and helping the cytologist to differentiate between reactive and malignant mesothelial cells in daily practice.

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