Influence of ageing, heat shock treatment and in vivo total antioxidant status on gene-expression profile and protein synthesis in human peripheral lymphocytes

2003 ◽  
Vol 124 (1) ◽  
pp. 55-69 ◽  
Author(s):  
D Visala Rao ◽  
Glen M Boyle ◽  
Peter G Parsons ◽  
Kenneth Watson ◽  
Graham L Jones
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Heat Shock ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Shock Treatment ◽  
Heat Shock Treatment ◽  
Total Antioxidant ◽  
Human Peripheral Lymphocytes

scholarly journals c-myc and c-myb protein degradation: effect of metabolic inhibitors and heat shock.

1988 ◽  
Vol 8 (6) ◽  
pp. 2504-2512 ◽  
Author(s):  
B Lüscher ◽  
R N Eisenman
Keyword(s):  
Protein Synthesis ◽  
Heat Shock ◽  
Ornithine Decarboxylase ◽  
Half Life ◽  
Shock Treatment ◽  
Metabolic Energy ◽  
Metabolic Inhibitors ◽  
Heat Shock Treatment ◽  
Myb Protein

The proteins encoded by both viral and cellular forms of the c-myc oncogene have been previously demonstrated to have exceptionally short in vivo half-lives. In this paper we report a comparative study on the parameters affecting turnover of nuclear oncoproteins c-myc, c-myb, and the rapidly metabolized cytoplasmic enzyme ornithine decarboxylase. The degradation of all three proteins required metabolic energy, did not result in production of cleavage intermediates, and did not involve lysosomes or ubiquitin. A five- to eightfold increase in the half-life of c-myc proteins, and a twofold increase in the half-life of c-myb proteins was detected after heat-shock treatment at 46 degrees C. In contrast, heat shock had no effect on the turnover of ornithine decarboxylase. Heat shock also had the effect of increasing the rate of c-myc protein synthesis twofold, whereas c-myb protein synthesis was decreased nearly fourfold. The increased stability and synthesis of c-myc proteins led to an overall increase in the total level of c-myc proteins in response to heat-shock treatment. Furthermore, treatments which reduced c-myc and c-myb protein turnover, such as heat shock and exposure to inhibitors of metabolic energy production, resulted in reduced detergent solubility of both proteins. The recovery from heat shock, as measured by increased turnover and solubility, was energy dependent and considerably more rapid in thermotolerant cells.

c-myc and c-myb protein degradation: effect of metabolic inhibitors and heat shock

1988 ◽  
Vol 8 (6) ◽  
pp. 2504-2512
Author(s):  
B Lüscher ◽  
R N Eisenman
Keyword(s):  
Protein Synthesis ◽  
Heat Shock ◽  
Ornithine Decarboxylase ◽  
Half Life ◽  
Shock Treatment ◽  
Metabolic Energy ◽  
Metabolic Inhibitors ◽  
Heat Shock Treatment ◽  
Myb Protein

The proteins encoded by both viral and cellular forms of the c-myc oncogene have been previously demonstrated to have exceptionally short in vivo half-lives. In this paper we report a comparative study on the parameters affecting turnover of nuclear oncoproteins c-myc, c-myb, and the rapidly metabolized cytoplasmic enzyme ornithine decarboxylase. The degradation of all three proteins required metabolic energy, did not result in production of cleavage intermediates, and did not involve lysosomes or ubiquitin. A five- to eightfold increase in the half-life of c-myc proteins, and a twofold increase in the half-life of c-myb proteins was detected after heat-shock treatment at 46 degrees C. In contrast, heat shock had no effect on the turnover of ornithine decarboxylase. Heat shock also had the effect of increasing the rate of c-myc protein synthesis twofold, whereas c-myb protein synthesis was decreased nearly fourfold. The increased stability and synthesis of c-myc proteins led to an overall increase in the total level of c-myc proteins in response to heat-shock treatment. Furthermore, treatments which reduced c-myc and c-myb protein turnover, such as heat shock and exposure to inhibitors of metabolic energy production, resulted in reduced detergent solubility of both proteins. The recovery from heat shock, as measured by increased turnover and solubility, was energy dependent and considerably more rapid in thermotolerant cells.

scholarly journals Characterization of the thermotolerant cell. I. Effects on protein synthesis activity and the regulation of heat-shock protein 70 expression.

1988 ◽  
Vol 106 (4) ◽  
pp. 1105-1116 ◽  
Author(s):  
L A Mizzen ◽  
W J Welch
Keyword(s):  
Protein Synthesis ◽  
Heat Shock ◽  
Stress Proteins ◽  
Stress Protein ◽  
Shock Treatment ◽  
Heat Shock Treatment ◽  
Normal Medium ◽  
Growth Environment ◽  
Mammalian Cell Lines ◽  
C 30

Exposure of mammalian cells to a nonlethal heat-shock treatment, followed by a recovery period at 37 degrees C, results in increased cell survival after a subsequent and otherwise lethal heat-shock treatment. Here we characterize this phenomenon, termed acquired thermotolerance, at the level of translation. In a number of different mammalian cell lines given a severe 45 degrees C/30-min shock and then returned to 37 degrees C, protein synthesis was completely inhibited for as long as 5 h. Upon resumption of translational activity, there was a marked induction of heat-shock (or stress) protein synthesis, which continued for several hours. In contrast, cells first made thermotolerant (by a pretreatment consisting of a 43 degrees C/1.5-h shock and further recovery at 37 degrees C) and then presented with the 45 degrees C/30-min shock exhibited considerably less translational inhibition and an overall reduction in the amount of subsequent stress protein synthesis. The acquisition and duration of such "translational tolerance" was correlated with the expression, accumulation, and relative half-lives of the major stress proteins of 72 and 73 kD. Other agents that induce the synthesis of the stress proteins, such as sodium arsenite, similarly resulted in the acquisition of translational tolerance. The probable role of the stress proteins in the acquisition of translational tolerance was further indicated by the inability of the amino acid analogue, L-azetidine 2-carboxylic acid, an inducer of nonfunctional stress proteins, to render cells translationally tolerant. If, however, analogue-treated cells were allowed to recover in normal medium, and hence produce functional stress proteins, full translational tolerance was observed. Finally, we present data indicating that the 72- and 73-kD stress proteins, in contrast to the other major stress proteins (of 110, 90, and 28 kD), are subject to strict regulation in the stressed cell. Quantitation of 72- and 73-kD synthesis after heat-shock treatment under a number of conditions revealed that "titration" of 72/73-kD synthesis in response to stress may represent a mechanism by which the cell monitors its local growth environment.

scholarly journals Ovarian Development and Vitellogenin Gene Expression under Heat Stress in Silkworm,Bombyx mori

2016 ◽  
Vol 2016 ◽  
pp. 1-8
Author(s):  
Satinath Paul ◽  
Bela Keshan
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Heat Shock ◽  
Reproductive Performance ◽  
Ovarian Development ◽  
Fat Body ◽  
Shock Treatment ◽  
Heat Shock Treatment ◽  
Vitellogenin Gene ◽  
Vitellogenin Mrna

The present study observed the effect of heat stress on ovarian development, fecundity, and vitellogenin gene expression in silkworm,Bombyx mori. The result showed that the heat shock treatment to spinning larvae and pupae at 39°C (1 h and 2 h) did not cause any adverse effect on the reproductive performance ofB. mori.However, the heat shock treatment at 42°C or above caused a decrease in the fecundity. The heat shock treatment to day 2 pupae for 2 h at 45°C caused a drastic effect on the development of ovary as measured by gonadosomatic index. The study thus showed that a brief exposure ofBombyxlarvae and pupae to a temperature of 42°C or higher, much prevalent in tropical countries like India, greatly affects the ovarian development and reproductive performance of this commercially important insect. The study further showed a developmental- and tissue-specific expression of vitellogenin mRNA in fat body and ovary upon heat shock. When heat shock treatment was done at 39°C and 42°C to spinning larvae, ovary showed an upregulation in the expression of vitellogenin mRNA, whereas fat body failed to do so. However, at 45°C, both fat body and ovary showed a downregulation. The heat shock treatment to day 2 pupae showed an upregulation in the vitellogenin mRNA expression in both fat body and ovary, even at 45°C. The upregulation in the expression of vitellogenin upon heat shock indicates its role in thermal protection ofBombyxlarvae and pupae.

scholarly journals IN VIVO ANALYSIS OF LIPID PEROXIDATION AND TOTAL ANTIOXIDANT STATUS IN SUBJECTS TREATED WITH STAINLESS STEEL ORTHODONTIC APPLIANCES

2013 ◽  
Vol 03 (03) ◽  
pp. 083-086
Author(s):  
Dilip Daniel Quadras ◽  
U.S. Krishna Nayak ◽  
Suchetha Kumari N. ◽  
Prashant Pujari ◽  
Vijay R. ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Stainless Steel ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Immunological Tolerance ◽  
Orthodontic Appliances ◽  
Time Interval ◽  
Total Antioxidant ◽  
Fixed Appliances

Abstract Introduction: Nickel is a strong immunologic sensitizer, although nickel sensitivity has been reported to be lower in subjects who have received orthodontic treatment; perhaps they develop immunological tolerance over the long period of treatment. Hence the present aim of the work was to determine the levels of lipid peroxidation and total antioxidant status in Serum and Saliva in subjects treated with stainless steel Orthodontic appliances. Methods and Materials: The study group included 25 participants and Samples were taken in different time intervals: Time interval 1- Collection of samples before the appliances fixed, Time interval 2- Collection of sample after one week of fixed appliances, Time interval 3- Collection of sample after three months of fixed appliances each from the Orthodontic Department of A. B.Shetty Memorial institute of dental science.Lipid peroxidation (Malondialdehyde) level is estimated using Thiobarbituric acid (TBA) method. Total antioxidant level was estimated using Phosphomolybdenum method. Conclusion: Total antioxidant and Malondialdehyde levels are increased in case of Females compared to Males in serum whereas in case of Saliva Total antioxidant level is increased in Time interval 2 and 3 in Males compared to Females.

scholarly journals Effects of commonly used medicinal herbs in Jordan on serum total antioxidant status and clinical laboratory testing

2019 ◽  
Vol 10 (4) ◽  
pp. 3489-3497
Author(s):  
Yousif. Y. Bilto ◽  
Nessrin. G. Alabdallat ◽  
Ali. M. Atoom ◽  
Noaman. A. Khalaf
Keyword(s):  
Oral Administration ◽  
Laboratory Testing ◽  
Antioxidant Status ◽  
Clinical Chemistry ◽  
Total Antioxidant Status ◽  
Medicinal Herbs ◽  
Aqueous Extracts ◽  
Total Antioxidant ◽  
Clinical Chemistry Tests

The use of medicinal herbs is widespread and growing. We tested the in-vivo effects of commonly used medicinal herbs in Jordan, on normal human volunteers after oral administration of aqueous extracts for several days, to see whether the in-vitro chemical antioxidant activity of a given herb replicates in-vivo and to see whether a given medicinal herb affects the results of selected clinical chemistry tests performed on the serum. Healthy volunteers were given orally aqueous herbal extracts daily for five days. Venous blood samples were taken before and one hour after the first dose of aqueous extract and then one day after the last dose of day five. Total antioxidant status and 13 of routine clinical chemistry tests were assayed on the serum. The tested herbs caused a significant increase in serum total antioxidant status. The studied herbs can be arranged in decreasing order of their in-vivo antioxidant strength after one hour of first consumption as follows: Nigella sativum > Rosmarinus officinalis > Zingiber officinale > Verbena triphylla > Origanum syriacum > Salvia triloba. The studied herbs except for Rosmarinus officinalis also caused a significant increase and/or decrease in 10 out of 13 laboratory tests performed on the serum. These results indicate that oral administration of aqueous extracts of medicinal herbs although improve the serum total antioxidant status, they could also significantly alter some laboratory results used for diagnosis or evaluation of diseases and this may negatively affect patient’s care. The present study highlights the importance of obtaining information regarding patients’ uptake of herbal products that might interfere with some laboratory testing.

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