Basic Life Support (BLS) training for school teachers is increasingly acknowledged as an important public health method to disseminate knowledge regarding life-saving skills such as CPR. However, there is a lack of studies examining this crucial area of training in Malaysia. Hence, this study aimed to investigate student teachers' knowledge, attitudes, and perceptions towards Basic Life Support training in a public university in Malaysia. An online questionnaire was implemented among final-year student teachers spanning seven different majors. Participants were required to answer 40 questions regarding their CPR knowledge, attitudes towards obtaining BLS training, and perceptions towards performing CPR. A total of 111 student teachers (80.2% female) responded to the survey. Results indicated that the majority of the participants (98.2%) would like to join a CPR training if offered. However, most participants reported a lack of information or resources in obtaining CPR training (82.0%) and had fears of incurring injury (93.7%), being sued (96.4%), being uncomfortable about bodily fluids (85.6%), or concerns of being infected with a communicable disease (85.6%) by the individual receiving CPR. Notably, knowledge regarding CPR was low regardless of CPR training history. Therefore, there is a need for public health information to be disseminated regarding CPR to dispel fears regarding its practice and provide future teachers opportunities to obtain BLS training.