Immunogold electron-microscopy localization of abscisic acid in flesh cells of grape berry (Vitis vinifera L.×Vitis labrusca L. cv Kyoho)

Ensaio foi conduzido com viderias da cultivar 'Niagara Rosada' (Vitis labrusca L. X Vitis vinifera L.) com 7 anos de idade, no município de Jundiaí, SP, (23°12' de latitude sul e 46°33' de longitude oeste e 715 m de altitude), situadas sobre um Regossolo unidade Currupira, com os objetivos de: (1) determinar as quantidades de nutrientes absorvidos pela videira nos diferentes estádios de desenvolvimento; (2) avaliar a exportação de nutrientes pela cultura durante um ciclo vegetativo. Após a brotação da videira, foram realizadas 17 coletas quinzenais de material. Foram coletadas e separadas as folhas das partes terminal e basal, sarmentos das partes terminal e basal e cachos. No material coletado foram determinados os teores de micronutrientes, com exceção do molibdênio e cloro. Curvas representativas das concentraçoes dos nutrientes nas partes da planta, em função da idade, foram obtidas a partir dos dados calculados através de equações de regressão. Pelos pontos de máximo estimaram-se as quantidades máximas de nutrientes extraídos. Concluiu-se que: - A concentração dos nutrientes é sempre maior nas folhas do que nos sarmentos e existem diferenças nas concentrações de nutrientes das folhas, sarmentos e cachos, em função da idade. - Os acúmulos máximos de nutrientes nas folhas, sarmentos e cachos ocorrem nas seguintes idades: - A exportação de nutrientes em mg por planta pelos cachos e sarmentos removidos pelas colheitas e poda é a seguinte.

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VvNAC17, a novel stress-responsive grapevine (Vitis vinifera L.) NAC transcription factor, increases sensitivity to abscisic acid and enhances salinity, freezing, and drought tolerance in transgenic Arabidopsis

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2019.11.002 ◽

2020 ◽

Vol 146 ◽

pp. 98-111 ◽

Cited By ~ 14

Author(s):

Yan-lun Ju ◽

Xiao-feng Yue ◽

Zhuo Min ◽

Xian-hang Wang ◽

Yu-lin Fang ◽

...

Keyword(s):

Transcription Factor ◽

Abscisic Acid ◽

Drought Tolerance ◽

Vitis Vinifera ◽

Transgenic Arabidopsis ◽

Nac Transcription Factor ◽

Vitis Vinifera L

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The molecular regulation of stilbene phytoalexin biosynthesis in Vitis vinifera during grape berry development

Functional Plant Biology ◽

10.1071/pp00007_co ◽

2000 ◽

Vol 27 (7) ◽

pp. 723 ◽

Cited By ~ 34

Author(s):

Anthony J. Bais ◽

Peter J. Murphy ◽

Ian B. Dry

Keyword(s):

Gene Expression ◽

Vitis Vinifera ◽

Stilbene Synthase ◽

Grape Berry ◽

Molecular Regulation ◽

Vitis Vinifera L ◽

Uv Treatment ◽

Grape Berries ◽

Uv Induction ◽

Phytoalexin Biosynthesis

The molecular regulation of stilbene phytoalexin biosynthesis in developing Vitis vinifera L. grape berries was investigated using a UV induction system. Berries were collected at 1, 5, 10 and 16 weeks post-flowering from the cultivars Shiraz, Semillon, Cabernet Sauvignon and Chardonnay and the skins analysed for resveratrol production following irradiation with UV-C light. The rate and maximal level of resveratrol accumulation increased markedly in berries sampled from 1–5 weeks post-flowering and then dramatically declined in maturing berries sampled from 10–16 weeks post-flowering in all cultivars. In berries sampled at 1 and 5 weeks post-flowering, maximal levels of resveratrol accumulation were recorded at incubation periods of 24 and 48 h respectively whereas maximal resveratrol levels were not recorded in week 16 berry skins until 72 h after UV-treatment. Gene expression analysis indicated that stilbene synthase (STS) mRNA accumulated within 4–8 h of UV treatment in berries sampled at 1 and 5 weeks post-flowering, but did not increase in week 16 berries until 24–48 h following UV-irradiation. Furthermore, the overall level of STS gene expression declined in berries sampled 10–16 weeks post-flowering. The results demonstrate that inducible stilbene accumulation in ripening grape berries is highly regulated at the level of STS gene transcription. This decline in inducible STS gene expression may be a major factor contributing to the increased susceptibility of ripening grape berries to Botrytis cinerea infection.

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