Effect of type and inclusion level of cold-pressed oilseed cakes on in vitro rumen fermentation

2014 ◽  
Vol 54 (10) ◽  
pp. 1709 ◽  
Author(s):  
H. Benhissi ◽  
A. García-Rodríguez ◽  
I. Beltrán de Heredia
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Barley Straw ◽  
High Fat ◽  
In Vitro Gas Production ◽  
Fat Level ◽  
Total Fat ◽  
Cold Pressed ◽  
In Vitro Rumen Fermentation

The current study was conducted to explore the impact of inclusion of different types and levels of cold-pressed oilseed cakes on in vitro rumen fermentation and gas production parameters. Two batch fermentation trials (24 and 96 h) were conducted using the in vitro gas production technique. In each trial, three types of lipid sources [palm fat (PF), cold-pressed rapeseed cake (CPRC) and cold-pressed sunflower cake (CPSC)] × two levels of total fat (30 and 60 g/kg DM) were evaluated. Incubated substrates were made from iso-energetic and isoproteic basal mixtures of barley straw and concentrate (ratio 10 : 90). During the 24-h incubation trial, samples were collected 24 h post-incubation to measure volatile fatty acids (VFA) and methane production. The 96-h incubation was used to assess the rate and extent of in vitro gas production. CPSC showed lower total VFA (P < 0.001) and methane (P < 0.001) production, compared with PF and CPRC. High fat level decreased total VFA (P < 0.001) and methane (P < 0.001) production compared with low level of inclusion. Molar proportions of individual VFA and acetate to propionate ratio were not affected by lipid supplementation. CPSC reduced (P = 0.006) asymptotic gas production at high fat level, compared with PF and CPRC. Neither supplementation level nor type of lipid affected the fractional gas production rate or lag time. In conclusion, there was no difference in ruminal fermentation when either CPRC or PF were added but CPSC altered microbial fermentation in vitro at a total fat level of 60 g/kg DM.

scholarly journals Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 108
Author(s):  
Yichong Wang ◽  
Sijiong Yu ◽  
Yang Li ◽  
Shuang Zhang ◽  
Xiaolong Qi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Acid Content ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Quadratic Response ◽  
In Vitro Rumen Fermentation

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

Comparison of four in vitro gas production methods to study rumen fermentation kinetics of starch rich feeds

1997 ◽  
Vol 1997 ◽  
pp. 196-196
Author(s):  
S. Fakhri ◽  
A. R. Moss ◽  
D.I. Givens ◽  
E. Owen
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Production Methods ◽  
Fermentation Kinetics ◽  
In Vitro Gas Production ◽  
Production Techniques ◽  
Kinetics Of

Recently, the automatic in vitro gas production techniques (e.g. Cone. 1994; Theodorou et al., 1994) have been developed to study rumen fermentation kinetics. Many approaches have been taken. This work investigates the suitability of different methods for estimating the rumen fermentation of two starch rich feedstuffs.

scholarly journals Effect of Eucalyptus globulus leaves extracts on in vitro rumen fermentation, methanogenesis, degradability and protozoa population

2018 ◽  
Vol 18 (3) ◽  
pp. 753-767 ◽  
Author(s):  
Amina Boussaada ◽  
Rabah Arhab ◽  
Serena Calabrò ◽  
Raffaella Grazioli ◽  
Maria Ferrara ◽  
...  
Keyword(s):  
Organic Matter ◽  
Ethyl Acetate ◽  
Eucalyptus Globulus ◽  
Ethyl Acetate Extract ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Aqueous Extracts ◽  
Ch4 Production ◽  
In Vitro Gas Production

Abstract The aim of the research was to evaluate the effect of three Eucalyptus globulus extracts rich in phenolic compounds, especially flavonoids, on rumen fermentation, methane (CH4) production, organic matter degradability and protozoa population using an in vitro gas production technique. Four concentrations (0, 50, 75 and 100 mg) of three Eucalyptus extracts (ethyl acetate, n-butanol and aqueous) were added to a diet of ruminants (forage: concentrate ratio 60:40) and incubated at 39°C under anaerobiosis with buffered rumen fluid. After 24 h, the fermentation fluid was analysed for ammonia-N and volatile fatty acids (VFA). Organic matter degradability (OMD) and protozoa were also determined; in vitro gas production was also recorded and CH4 concentration was measured. Compared to the control, CH4 production was significantly lower for ethyl acetate extract (P<0.05), but higher for n-butanol and aqueous extracts. Production of ammonia- N was lower in all Eucalyptus extracts (P<0.05). Propionate production (P<0.05) increased for ethyl acetate and n-butanol extracts, whereas no effect was registered for VFA, for all Eucalyptus extracts. Ethyl acetate extract decreased in vitro OMD (P<0.05), whereas n-butanol and aqueous extracts were comparable to the control. Protozoa population decreased (P<0.05) for all extracts in comparison with the control. Eucalyptus ethyl acetate extract might be promising to be used as a potent anti-methanogenic additive. Moreover, the assessment of the right dosage seems to be important to decrease methane production, without reducing feed nutritional value.

scholarly journals Rumen fermentation kinetics as impacted by red amaranth (Amaranthus cruentus, L) leaf powder using in vitro gas production technique

2020 ◽  
Author(s):  
Thiwakorn Ampapon ◽  
Bounnaxay Viennasay ◽  
Metha Wanapat
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Amaranthus Cruentus ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Two Factors ◽  
Dry Matter Degradation ◽  
Leaf Powder ◽  
Completely Randomized Design

Abstract Background A need for research searching for alternative rumen enhancers warrants immediate attention. The in vitro fermentation experiment was conducted using factorial arrangement of two factors of roughage to concentrate and seven level of red amaranth leaf powder percentage of total substrate in a Completely randomized design (CRD). Two factors, namely Factor A was two ratio of roughage (R) to concentrate (C) at 60:40 and 40:60 and Factor B was level of red amaranth (Amaranthus cruentus, L) leaf powder (RALP) supplementation at 0, 2, 4, 6, 8, 10, and 12% of total dietary substrate. Results Red amaranth leaf powder (RALP) contained phytonutrients both condensed tannins and saponins in addition with high macro minerals (Ca, K, and Mg). This experiment revealed innovations of the RALP supplementation by enhancing rumen propionate (C3) production, reducing acetate (C2) to (C3) ratio, reducing protozoal population and mitigating methane (CH4) production. Furthermore, rumen dry matter degradation percentages were remarkably enhanced (P < 0.001) by increasing RALP supplementation. Conclusion Plants rich in phytonutrients and minerals such as red amaranth leaf powder (RALP) have a vital and promising role in modulating rumen fermentation, mitigating methane production, as well as increasing substrate degradability.

scholarly journals Nutritional Quality and In Vitro Rumen Fermentation Characteristics of Silage Prepared with Lucerne, Sweet Maize Stalk, and Their Mixtures

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1205
Author(s):  
Musen Wang ◽  
Fujin Zhang ◽  
Xinxin Zhang ◽  
Ying Yun ◽  
Lei Wang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Nutritional Quality ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Fresh Matter ◽  
In Vitro Rumen Fermentation

The objective of this work was to evaluate the pH, chemical composition, minerals, vitamins, and in vitro rumen fermentation characteristics of silage prepared with lucerne, sweet maize stalk (MS), and their mixtures. Freshly chopped lucerne and MS were combined in ratios of 100:0 (M0, control), 80:20 (M20), 60:40 (M40), 40:60 (M60), 20:80 (M80), and 0:100 (M100) on a fresh matter basis. Each treatment was prepared in triplicate, and a total of eighteen silos were fermented for 65 days. After 65 days of fermentation, the pH values in M0, M20, M40, M60, M80, and M100 silages were 5.47, 4.84, 4.23, 4.13, 3.79, and 3.61, respectively. As the MS proportion in the mixtures increased, silage K, Ca, P, Na, Fe, and Cu concentrations linearly decreased (p < 0.001) and so did vitamins B5 and K1 and α-tocopherol. In vitro rumen dry matter and organic matter degradability, pH, ammonia, total volatile fatty acid, and gas production linearly decreased (p < 0.01), while neutral detergent fiber concentration linearly increased (p < 0.001), with increasing proportion of MS. The in vitro dry matter and organic matter degradability rapidly decreased when the MS percentage was ≥60%. In conclusion, the M40 silage is the most suitable for livestock utilization in local forage production considering the balance of silage pH, nutritional quality, and in vitro ruminal fermentation characteristics.

The effect of bromoethanesulphonic acid on gas production during an in vitro rumen fermentation

1998 ◽  
Vol 22 ◽  
pp. 193-194
Author(s):  
D. R. Davies ◽  
E. L. Bakewell ◽  
D. K. Leemans ◽  
R. J. Merry
Keyword(s):  
Pressure Transducer ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Production Technique ◽  
Microbial Ecosystem ◽  
Considerable Potential ◽  
Microbial Ecosystems ◽  
Presence And Absence ◽  
In Vitro Rumen Fermentation

Pressure transducer technology to measure gas production from microbial ecosystems has been utilized in a number of ways but predominantly for food evaluation. The approach also has considerable potential to increase our understanding of, and ability to manipulate, the rumen microbial ecosystem, but most research to date has concentrated on measurement of total gas production and not composition. The aim of this study was to extend the scope of the gas production technique to the quantitation of component gases, whilst investigating ruminal gas production in the presence and absence of methanogens.

scholarly journals Methanolic extract of Urochloa humidicola on in vitro rumen fermentation

2018 ◽  
Vol 53 (4) ◽  
pp. 504-513
Author(s):  
Rafaela Scalise Xavier de Freitas ◽  
Delci de Deus Nepomuceno ◽  
Elisa Cristina Modesto ◽  
Tatiana Pires Pereira ◽  
João Carlos de Carvalho Almeida ◽  
...  
Keyword(s):  
Dry Matter ◽  
Methanolic Extract ◽  
Short Chain Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Short Chain ◽  
Control Group ◽  
Fatty Acid Production ◽  
In Vitro Rumen Fermentation

Abstract: The objective of this work was to evaluate the effect of the addition of the methanolic extract of Urochloa humidicola at four different concentrations (0, 75, 150, and 250 g L-1) on the in vitro rumen fermentation of Urochloa brizantha. The following variables were evaluated by the in vitro gas production technique: kinetic parameters; rumen degradation of dry matter; and production and concentration of the methane and carbon dioxide gases and of the acetate, propionate, and butyrate short-chain fatty acids. The addition of the methanolic extract reduces the production of gases generated from the degradation of non-fibrous carbohydrates (fraction A) in 9.55, 6.67, and 13.33%, respectively, at the concentrations of 75, 150, and 250 g L-1, compared with the control group, but it negatively affects the degradation of the dry matter of U. brizantha at the concentrations of 150 and 250 g L-1. The extract shows negative quadratic effect on gas production during 12 and 24 hours of U. brizantha incubation. The extract of U. humidicola reduces methane production and increases short-chain fatty acid production at the concentrations of 75, 150, and 250 g L-1.

Dietary dragon fruit (Hylocereus undatus) peel powder improved in vitro rumen fermentation and gas production kinetics

2019 ◽  
Vol 51 (6) ◽  
pp. 1531-1538 ◽  
Author(s):  
Maharach Matra ◽  
Metha Wanapat ◽  
Anusorn Cherdthong ◽  
Suban Foiklang ◽  
Chaowarit Mapato
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Production Kinetics ◽  
Dragon Fruit ◽  
Hylocereus Undatus ◽  
In Vitro Rumen Fermentation
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