Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds

2004 ◽  
Vol 111 (1-4) ◽  
pp. 57-71 ◽  
Author(s):  
G Getachew ◽  
P.H Robinson ◽  
E.J DePeters ◽  
S.J Taylor
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Gas Production ◽  
In Vitro Gas Production ◽  
Dry Matter Degradation

scholarly journals Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 60-67 ◽  
Author(s):  
A. Kamalak ◽  
O. Canbolat ◽  
Y. Gurbuz ◽  
O. Ozay
Keyword(s):  
Dry Matter ◽  
Degradation Kinetics ◽  
Insoluble Fraction ◽  
Gas Production ◽  
Barley Straw ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Dry Matter Degradation

Dry matter (DM) degradation of wheat straw (WS), barley straw (BS), lucerne hay (LH) and maize silage (MS) was determined using two different techniques: (i) in vitro gas production and (ii) nylon bag degradability technique. In vitro gas production and in situ DM disappearance were measured after 3, 6, 12, 24, 48, 72 and 96 hours of incubation. In situ and in vitro DM degradation kinetics was described using the equation y = a + b (1 &ndash; e<sup>ct</sup>). In all incubations there were significant (P &lt; 0.001) correlations between gas production and in situ DM disappearance or estimated parameters ((a + b)<sub>ga</sub><sub>s</sub> and (a + b)<sub>is</sub> or (a + b)<sub>gas</sub> and EDMD<sub>is</sub>) whereas there were no significant (P &gt; 0.05) correlations between c<sub>gas</sub> and c<sub>is</sub> or b<sub>gas</sub> and b<sub>is</sub>. Gas production from the insoluble fraction (b) alone explained 98.3% of the variation of EDMD. The inclusion of gas production from the quickly soluble fraction (a) and rate constant (c) of gas production in the regression equation improved the accuracy of EDMD prediction. The correlations between the results of both methodologies seem to be sufficiently strong to predict degradability parameters from gas production parameters. It was concluded that the in vitro gas production technique has good potentiality to predict in situ DM disappearance and some DM degradation parameters. &nbsp; &nbsp;

Comparison between in vitro gas production and in situ degradation for evaluating rumen dry matter degradation of maize grain and tropical grasses

2006 ◽  
Vol 34 ◽  
pp. 69-72
Author(s):  
R. M. Mauricio ◽  
T.R. Tomich ◽  
R. A. Filho ◽  
R. B. Reis ◽  
L.C. Gonçalves ◽  
...  
Keyword(s):  
Closed System ◽  
Dry Matter ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Dry Matter Degradation ◽  
In Situ Degradation ◽  
Maize Grain

SummaryThe in situ technique (NB) was compared to the in vitro gas production technique (Gas) in terms of ability to estimate the dry matter degradation (DMD) using high soluble substrates (maize grains) and low soluble substrates (four tropical forages). The experiments (in situ and in vitro) were carried out at the same time using the same cow for both techniques and DMD was estimated at 6, 12, 24, 48 & 96 h. The results showed that DMD from Gas were lower than NB DMD and the correlation for the maize group were lower than those of the grass group which indicated that Gas technique, closed system, has potential to analyse highly soluble substrates, probably, overcoming the particle losses effect demonstrated by the in situ technique.

scholarly journals Effect of the ruminal liquid extraction method in the in vitro gas technique

2021 ◽  
Vol 38 (3) ◽  
pp. 681-691
Author(s):  
Roselia Ramírez Díaz ◽  
René Pinto Ruiz ◽  
Francisco Guevara Hernández ◽  
José Venegas Venegas ◽  
Mariela Reyes Sosa ◽  
...  
Keyword(s):  
Dry Matter ◽  
Elaeis Guineensis ◽  
Liquid Extraction ◽  
Gas Production ◽  
Extraction Technique ◽  
Fluid Extraction ◽  
Ruminal Fluid ◽  
In Vitro Gas Production ◽  
Dry Matter Degradation

In vitro gas studies require the use of cannulated animals; however, the high cost of the surgery limits its application. Therefore, the objective of the study was to evaluate the effect of the ruminal fluid extraction technique by oro-ruminal probe affects the results derived from the in vitro gas production technique (GPT). Elaeis guineensis meal was used as a substrate. Ruminal fluid was obtained in two ways: cannulated animals and oro-ruminal probe. Dry matter degradation was estimated (DMD), parameters of fermentation kinetics and fractional volume by the GPT. The comparison of means was carried out using the Tukey procedure (p<0,05). The results indicated the lack of significant statistical differences (p>0,05). It was concluded that the ruminal fluid extraction technique did not modify the DGRMS, the parameters and fermentation fractions of E. guineensis.

scholarly journals Preserving Porphyra umbilicalis and Saccharina latissima as Silages for Ruminant Feeding

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1957
Author(s):  
Margarita Novoa-Garrido ◽  
Carlos Navarro Marcos ◽  
María Dolores Carro Travieso ◽  
Eduarda Molina Alcaide ◽  
Mogens Larsen ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Saccharina Latissima ◽  
Porphyra Umbilicalis ◽  
In Vitro Gas Production ◽  
Ruminal Degradability ◽  
Kinetics Of

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH3-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants.

Condensed tannins extracted from Quebracho affect the fermentation profile, nutritional quality and in vitro gas production of pornunça (Manihot spp.) silages

2021 ◽  
pp. 1-10
Author(s):  
D. T. Q. Carvalho ◽  
A. R. F. Lucena ◽  
T. V. C. Nascimento ◽  
L. M. L. Moura ◽  
P. D. R. Marcelino ◽  
...  
Keyword(s):  
Nutritional Quality ◽  
Dry Matter ◽  
Condensed Tannin ◽  
Gas Production ◽  
Rapid Decline ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Acid Detergent Fibre ◽  
Fermentation Profile

Abstract The objective was to evaluate the fermentation profile, in vitro gas production and nutritional quality of pornunça (Manihot spp.) silages containing levels of condensed tannin (CT; 0, 4, 8 and 12% on dry matter (DM) basis), at five opening times (0, 3, 7, 14, 28 and 56 days). A completely randomized design in a 4 × 5 factorial arrangement was adopted, with four replications, totalling 80 experimental silos. The pH and NH3-N analyses were performed at all opening times of the silos. The other analyses were performed only with silages opened at 56 days of storage. There was an interaction effect between CT levels and silo opening times for pH and NH3-N. Tannin levels in pornunça silages after 56 days ensiling increased the pH and DM and reduced crude protein (CP) and neutral detergent fibre (NDF). There was a quadratic effect for NH3-N, acetic acid, butyric acid, gas losses, dry matter recovery (DMR), hemicellulose and acid detergent fibre. Inclusion of 4 and 8% CT in pornunça silage promotes a rapid decline in pH, being within the acceptable limit for adequate fermentation at 3 days of ensiling. Silages with 4% CT establish the pH at 28 days of opening the silos, with reduced NH3-N. Silages with 4% CT present higher concentrations of acetic and butyric acids and greater DMR. Inclusion of CT in pornunça silage after 56 days ensiling increases DM and reduces CP and NDF, directly affecting the in vitro degradability and reducing gas production.

scholarly journals The nutritional evaluation of forage-based mixed rations in New Zealand using an in vitro gas production technique. 1: analytical survey

2021 ◽  
pp. 1-6
Author(s):  
N.D. Meads ◽  
R. Tahmasbi ◽  
N. Jantasila
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Environmental Science ◽  
Ghg Emissions ◽  
Negative Relationship ◽  
Real Data ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production

Greenhouse gas (GHG) emissions from livestock are an important consideration in environmental science. Estimating GHG production can be problematic at a farm or animal level, and requires controlled conditions to produce real data. An in vitro gas production technique (IVGPT) was developed to evaluate forage-based total mixed rations in digestion kinetics and GHG production. Two hundred and sixty samples of complete mixed rations (MR), which included a pasture component used in commercial lactating dairy herds, were collected around NZ across three calendar years, 2017-2019. Twenty of the 260 samples were 100% total mixed rations (TMR) with no pasture content. The samples were submitted for proximate analysis as well as IVGPT to generate GHG production figures. The results showed an average total gas production (TGP) of 129.82 ml/g dry matter (DM), 78.6% true digestibility (TDMD), 125.06 mg/g DM microbial biomass (MB), 20.16 g CH4/kg DM, and 12.8 MJME/kg DM. The average nutrient composition was dry matter (DM) 31.55%, crude protein (CP) 21.85%, neutral detergent fibre (NDF) 44.35%, and starch 7.03%. The IVGPT CH4 production was negatively correlated to NDF (r=-0.312), ADF (r=-0.193), TGP (r=-0.216), and was positively correlated with TDMD (r=0.250), apparent digestibility (ADMD) (r=0.614), starch (r=0.117) and volatile fatty acids (r=0.538). The MR diet showed a strong positive relationship with ADMD digestibility (P=0.01) and a negative relationship with fibre content (NDF, P=0.01 and ADF, P=0.01). However, CH4 production reduced linearly with increasing TGP (P=0.01). The results indicated that a greater CH4 production may be related to higher digestibility of mixed ration.

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