Assessment of rumen in vitro incubation (gas production) technique and chemical analyses by detergent system to predict metabolisable energy content in mixed diets of lactating cows

1998 ◽  
Vol 74 (2) ◽  
pp. 169-177 ◽  
Author(s):  
M.B Nataraja ◽  
U Krishnamoorthy ◽  
P Krishnappa
Keyword(s):  
Energy Content ◽  
Gas Production ◽  
Chemical Analyses ◽  
Production Technique ◽  
Lactating Cows ◽  
In Vitro Incubation ◽  
Metabolisable Energy ◽  
Detergent System

Chemical composition and metabolisable energy content of four aquatic plants for sheep

2005 ◽  
Vol 2005 ◽  
pp. 153-153
Author(s):  
M. Sakarya ◽  
A. Kamalak ◽  
O. Canbolat ◽  
Y. Gurbuz ◽  
N. Tursun ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Aquatic Plants ◽  
Energy Content ◽  
Gas Production ◽  
Production Technique ◽  
Lack Of Information ◽  
Nutritive Values ◽  
Ruminant Diets ◽  
Metabolisable Energy

Although some aquatic plants have been used for ruminant diets in some parts of Turkey there is a lack of information about their nutritive values. The aim of this study was to determine the chemical composition and metabolisable energy (ME) content of four different aquatic plants using in vitro gas production technique.

Effects of increasing the inclusion level of Atriplex amnicola in the diet of sheep

2009 ◽  
Vol 49 (11) ◽  
pp. 1029
Author(s):  
M. B. P. Kumara Mahipala ◽  
G. L. Krebs ◽  
P. McCafferty ◽  
K. Dods
Keyword(s):  
Crude Protein ◽  
Energy Content ◽  
Gas Production ◽  
Mineral Contents ◽  
Neutral Detergent Fibre ◽  
Freeze Dried ◽  
Acid Detergent Fibre ◽  
Inclusion Level ◽  
Metabolisable Energy

The effects of increasing the level of inclusion of fresh Atriplex amnicola Paul G.Wilson foliage in an oaten chaff (Avena sativa L.) diet fed to sheep were investigated. Six experimental diets were formulated to contain varying amounts [0, 161, 362, 496, 650 and 836 g/kg diet dry matter (DM)] of A. amnicola, and these diets were fed to individually penned sheep according to a Latin square design. Feed and faecal samples were analysed for crude protein, neutral detergent fibre, acid detergent fibre, acid detergent lignin, and ash contents. Mineral contents and 24 h in vitro gas production were measured in freeze-dried feed samples. As the level of inclusion of A. amnicola increased, digestibility of DM, organic matter and neutral detergent fibre increased (P < 0.05), reaching the peak at the 496 g/kg DM inclusion level and then decreasing (P < 0.05) thereafter. Acid detergent fibre digestibility followed a similar trend, but peak digestibility was at 362 g/kg DM inclusion level. Digestibility of crude protein continued to increase (P < 0.05) with increasing inclusion of A. amnicola. Gas production (24 h) from A. amnicola was lower (P < 0.05) than that from oaten chaff (24.4 v. 45.3 mL/200 mg DM) and also had lower metabolisable energy content (in vitro ME, 6.3 v. 8.6 MJ/kg DM). As the level of inclusion of A. amnicola in the diet increased, gas production and in vitro metabolisable energy content decreased (P < 0.05). The 496 g/kg DM inclusion level provided the optimum N : energy ratio for fermentation of dietary fibre. At this level of inclusion the diet was rich in Na, Ca, P, Mg, K, S, Mn and Zn. High inclusion levels improved the crude protein value of the diet but compromised fibre digestibility.

Variation In Metabolizable Energy Content of Forages Estimated Using in vitro Gas Production Technique

2004 ◽  
Vol 7 (4) ◽  
pp. 601-605 ◽  
Author(s):  
A. Kamalak . ◽  
O. Canbolat . ◽  
Y. Gurbuz . ◽  
O. Ozay . ◽  
E. Ozkose .
Keyword(s):  
Energy Content ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Production Technique ◽  
In Vitro Gas Production

Associative effects of poor-quality forages combined with food industry byproducts determined in vitro with an automated gas-production system

2015 ◽  
Vol 55 (9) ◽  
pp. 1117 ◽  
Author(s):  
Franco Tagliapietra ◽  
Mirko Cattani ◽  
Matteo Guadagnin ◽  
Mohamed L. Haddi ◽  
Leonardo Sulas ◽  
...  
Keyword(s):  
Food Industry ◽  
Energy Content ◽  
Rumen Fluid ◽  
Poor Quality ◽  
Gas Production ◽  
Apple Pomace ◽  
Economic Sustainability ◽  
Citrus Pulp ◽  
Metabolisable Energy

This experiment aimed to investigate the associative effects among two low-quality forages (crown daisy, milk thistle) and three agro-industrial byproducts (apple pomace, citrus pulp, tomato peel), by means of an automated gas production (GP) system. All feeds were incubated alone or as 50 : 50 mixtures of each forage with each byproduct. Samples (0.500 ± 0.0010 g) of single feeds or mixtures were incubated for 96 h, in three replicates in individual bottles (310 mL), with 75 mL of buffered rumen fluid. Bottles were vented by an open-close valve when the internal pressure reached 3.4 kPa. The metabolisable energy content of single feeds and mixtures was computed from GP at 24 h and feed chemical composition. Feed substrates were ranked for GP in the following way: byproducts, mixtures, and forages. The two forages did not differ for GP and metabolisable energy content, although differences were observed among byproducts and among mixtures. Both forages interacted positively with apple pomace from 6 h (P < 0.001) to 24 h (P = 0.029) of incubation and with citrus pulp at 12 h (P = 0.005) and 24 h (P = 0.012), whereas no associative effects were detected when forages were incubated with tomato peels. Results suggest that in vitro fermentability of low-quality forages could be efficiently improved by combining these two forages with apple pomace or citrus pulp. These findings are relevant, because the use of low-quality forages and byproducts in ruminant feeding is considered important for improving the environmental and economic sustainability of forage systems in arid and semi-arid areas.

The gas production capacity of purified chemicals and feedstuffs when incubated in vitro with rumen microbes as a possible indicator of energy availability in the rumen

1994 ◽  
Vol 1994 ◽  
pp. 91-91
Author(s):  
A.J. Chamberlain
Keyword(s):  
Microbial Activity ◽  
Energy Content ◽  
Production Capacity ◽  
Gas Production ◽  
Energy Availability ◽  
Rumen Microbes ◽  
Available Energy ◽  
Metabolisable Energy ◽  
Theoretical Considerations

The metabolisable protein system (AFRC, 1992) introduced the term Fermentable Metabolisable Energy (FME) as a measure of the amount of energy that was available in the rumen to support microbial activity. FME is currently derived from theoretical considerations rather than direct measurement; it is on based metabolisable energy (ME) but does not take into account rumen outflow rates and gives limited consideration to the chemical composition of feeds. Gas production from in vitro fermentation of feeds is an indicator of microbial activity which might be a suitable assessment of the rumen available energy content of feeds.

scholarly journals Effect of tannin from blackberry (Syzigium cumini) seed on in vitro rumen fermentation

2015 ◽  
Vol 25 ◽  
pp. 31-37
Author(s):  
SCK Barma ◽  
MR Amin ◽  
M Monirruzzaman ◽  
MB Sarker ◽  
AKMA Kabir
Keyword(s):  
Organic Matter ◽  
Energy Content ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Feed Additives ◽  
In Vitro Gas Production ◽  
Metabolisable Energy ◽  
Organic Matter Digestibility ◽  
Fermentation Parameters

An experiment was conducted to quantify the chemical composition, different forms of tannins and their effects on in vitro gas production, organic matter digestibility and metabolisable energy content in Syzigium cumini (Blackberry) seed with and without polyethelene glycol (PEG). The DM, OM, ash, CP, NDF and ADF contents of S. cumini seeds were 90.1, 87.6, 2.6, 4.6, 32.9 and 15.5%, respectively. The total phenol, total tannin, condensed tannin and hydrolysable tannin content were 5.89, 4.25, 0.42 and 0.05mg/g seed, respectively. In vitro gas production, organic matter digestibility and metabolisable energy content of S. cumini seed were 54 ml, 42.91% and 6.43 MJ/Kg DM, respectively. Addition of PEG to tannin containing seed was significantly (P<0.05) increased in vitro gas production, OMD and ME content. Higher levels of tannins in S. cumini seed could limit utilization through impaired digestibility and nutrient utilization but the addition of PEG increased all the fermentation parameters studied. It is predictable from the present findings that S. cumini seed could be utilized as natural source of feed additives to alter rumen fermentation parameters especially to protect protein and other nutrients from ruminal degradation, thereby make the nutrients available in the lower tract for higher milk and meat yield.Progress. Agric. 2014. 25: 31-37

Estimation of fermentable energy by the automatic in vitro gas production technique for a selection of feedstuffs incubated alone and in combination

1998 ◽  
Vol 1998 ◽  
pp. 69-69
Author(s):  
S. Fakhri ◽  
A. R. Moss ◽  
D.I. Givens ◽  
E. Owen
Keyword(s):  
Organic Matter ◽  
Incubation Period ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Fe Content ◽  
The Individual ◽  
Gas Volume ◽  
Selection Of

The gas production (GP) technique has previously been used to estimate the gas volume (fermentable energy (FE)) of compound feed ingredients for ruminants (Newbold et al., 1996). It was shown that the FE content of feed mixtures was represented by the combination of the total gas from the incubation of the individual feeds. However this additivity might not be consistent throughout the incubation period. The objectives were to test whether 1. other GP parameters give better estimates of FE for simple mixtures and are they additive; 2. whether organic matter apparently degraded in the rumen (OMADR) explain differences in GP; and 3. to find out if there are any other better measures than OMADR for estimating FE.

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