Associative effects of poor-quality forages combined with food industry byproducts determined in vitro with an automated gas-production system

2015 ◽  
Vol 55 (9) ◽  
pp. 1117 ◽  
Author(s):  
Franco Tagliapietra ◽  
Mirko Cattani ◽  
Matteo Guadagnin ◽  
Mohamed L. Haddi ◽  
Leonardo Sulas ◽  
...  
Keyword(s):  
Food Industry ◽  
Energy Content ◽  
Rumen Fluid ◽  
Poor Quality ◽  
Gas Production ◽  
Apple Pomace ◽  
Economic Sustainability ◽  
Citrus Pulp ◽  
Metabolisable Energy

This experiment aimed to investigate the associative effects among two low-quality forages (crown daisy, milk thistle) and three agro-industrial byproducts (apple pomace, citrus pulp, tomato peel), by means of an automated gas production (GP) system. All feeds were incubated alone or as 50 : 50 mixtures of each forage with each byproduct. Samples (0.500 ± 0.0010 g) of single feeds or mixtures were incubated for 96 h, in three replicates in individual bottles (310 mL), with 75 mL of buffered rumen fluid. Bottles were vented by an open-close valve when the internal pressure reached 3.4 kPa. The metabolisable energy content of single feeds and mixtures was computed from GP at 24 h and feed chemical composition. Feed substrates were ranked for GP in the following way: byproducts, mixtures, and forages. The two forages did not differ for GP and metabolisable energy content, although differences were observed among byproducts and among mixtures. Both forages interacted positively with apple pomace from 6 h (P < 0.001) to 24 h (P = 0.029) of incubation and with citrus pulp at 12 h (P = 0.005) and 24 h (P = 0.012), whereas no associative effects were detected when forages were incubated with tomato peels. Results suggest that in vitro fermentability of low-quality forages could be efficiently improved by combining these two forages with apple pomace or citrus pulp. These findings are relevant, because the use of low-quality forages and byproducts in ruminant feeding is considered important for improving the environmental and economic sustainability of forage systems in arid and semi-arid areas.

scholarly journals Determination of in vitro rumen digestibility and potential feed value of tiger nut varieties

2021 ◽  
Vol 50 (5) ◽  
Author(s):  
T. Ayaşan ◽  
E. Sucu ◽  
I. Ülger ◽  
H. Hızlı ◽  
P. Cubukcu ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Nutritive Value ◽  
Energy Content ◽  
Rumen Fluid ◽  
Gas Production ◽  
Time Dependent ◽  
In Vivo Studies ◽  
In Vitro Digestibility ◽  
In Vitro Gas Production

Tiger nut (Cyperus esculentus L.), or chufa, is a plant that is found in nature and is cultivated for its edible tubers. The purpose of this study was to determine the chemical composition, nutritive value, and in vitro digestibility of three tiger nut varieties using the in vitro gas production technique. These varieties were Sarışeker (yellow), Introduction 1, and Balyumru (brown). Rumen fluid was obtained from two cannulated Holstein animals. Time-dependent in vitro gas production was monitored at 3, 6, 9, 12, 24, 48, 72, and 96 hours of incubation. The varieties differed in dry matter (DM), crude ash (CA), ether extract (EE), neutral detergent fibre (NDF), and non-fibre carbohydrate (NFC) content (P <0.05). They also differed in the instantaneous volume of gas produced and in time-dependent gas production. Balyumru produced more gas at the onset of incubation than Introduction 1 and Sarışeker. However, over time, the gas produced by digestion of Introduction 1 exceeded the other two varieties. The amounts of gas produced at each time-point were intercorrelated. It is recommended that these results should lead to further evaluation in in vivo studies. Keywords: chemical composition, energy content, in vitro gas production

Chemical composition and metabolisable energy content of four aquatic plants for sheep

2005 ◽  
Vol 2005 ◽  
pp. 153-153
Author(s):  
M. Sakarya ◽  
A. Kamalak ◽  
O. Canbolat ◽  
Y. Gurbuz ◽  
N. Tursun ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Aquatic Plants ◽  
Energy Content ◽  
Gas Production ◽  
Production Technique ◽  
Lack Of Information ◽  
Nutritive Values ◽  
Ruminant Diets ◽  
Metabolisable Energy

Although some aquatic plants have been used for ruminant diets in some parts of Turkey there is a lack of information about their nutritive values. The aim of this study was to determine the chemical composition and metabolisable energy (ME) content of four different aquatic plants using in vitro gas production technique.

Effects of increasing the inclusion level of Atriplex amnicola in the diet of sheep

2009 ◽  
Vol 49 (11) ◽  
pp. 1029
Author(s):  
M. B. P. Kumara Mahipala ◽  
G. L. Krebs ◽  
P. McCafferty ◽  
K. Dods
Keyword(s):  
Crude Protein ◽  
Energy Content ◽  
Gas Production ◽  
Mineral Contents ◽  
Neutral Detergent Fibre ◽  
Freeze Dried ◽  
Acid Detergent Fibre ◽  
Inclusion Level ◽  
Metabolisable Energy

The effects of increasing the level of inclusion of fresh Atriplex amnicola Paul G.Wilson foliage in an oaten chaff (Avena sativa L.) diet fed to sheep were investigated. Six experimental diets were formulated to contain varying amounts [0, 161, 362, 496, 650 and 836 g/kg diet dry matter (DM)] of A. amnicola, and these diets were fed to individually penned sheep according to a Latin square design. Feed and faecal samples were analysed for crude protein, neutral detergent fibre, acid detergent fibre, acid detergent lignin, and ash contents. Mineral contents and 24 h in vitro gas production were measured in freeze-dried feed samples. As the level of inclusion of A. amnicola increased, digestibility of DM, organic matter and neutral detergent fibre increased (P < 0.05), reaching the peak at the 496 g/kg DM inclusion level and then decreasing (P < 0.05) thereafter. Acid detergent fibre digestibility followed a similar trend, but peak digestibility was at 362 g/kg DM inclusion level. Digestibility of crude protein continued to increase (P < 0.05) with increasing inclusion of A. amnicola. Gas production (24 h) from A. amnicola was lower (P < 0.05) than that from oaten chaff (24.4 v. 45.3 mL/200 mg DM) and also had lower metabolisable energy content (in vitro ME, 6.3 v. 8.6 MJ/kg DM). As the level of inclusion of A. amnicola in the diet increased, gas production and in vitro metabolisable energy content decreased (P < 0.05). The 496 g/kg DM inclusion level provided the optimum N : energy ratio for fermentation of dietary fibre. At this level of inclusion the diet was rich in Na, Ca, P, Mg, K, S, Mn and Zn. High inclusion levels improved the crude protein value of the diet but compromised fibre digestibility.

Metabolisable Energy Content of a Saponified Palm Fat Product for Ruminants

1988 ◽  
Vol 1988 ◽  
pp. 22-22
Author(s):  
D I Givens ◽  
Jeannie M Everington ◽  
N Shepperson
Keyword(s):  
Fatty Acids ◽  
Cell Walls ◽  
Energy Content ◽  
Rumen Fluid ◽  
Calcium Soap ◽  
Ruminant Diets ◽  
Soya Oil ◽  
Metabolisable Energy ◽  
Rumen Microflora

Many experiments have shown (see Armstrong and Ross, 1968) that the addition of fats to ruminant diets can lead to a reduction in the digestibility of forage cell walls due to adverse affects on the rumen microflora. Jenkins and Palmquist (1982) showed in vitro that the formation of insoluble calcium soaps in rumen fluid could prevent this problem. Additionally, Jenkins and Palmquist (1984) reported that the feeding of the preformed calcium soaps of tallow and soya oil fatty acids to dairy cows allowed normal rumen digestion of fibre whereas non-saponified tallow fatty acids caused a reduction in fibre digestion.The main aims of the present experiment were to examine a new calcium soap of palm fatty acids for its effects on whole tract digestibility of various feed fractions and to measure its effective metabolisable energy (ME) content at maintenance, the present United Kingdom standard.

scholarly journals Chemical Characterisation and in Vitro Gas Production Kinetics of Eight Faba Bean Varieties

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 398
Author(s):  
Alessandra Pelagalli ◽  
Nadia Musco ◽  
Nikita Trotta ◽  
Monica I. Cutrignelli ◽  
Antonio Di Francia ◽  
...  
Keyword(s):  
Faba Bean ◽  
Energy Content ◽  
Rumen Fluid ◽  
Morphological Characteristics ◽  
Gas Production ◽  
In Vitro Fermentation ◽  
In Vitro Gas Production ◽  
Ruminant Diets ◽  
Fermentation Parameters

Faba bean is an important vegetable protein source for ruminant diets. This research aimed to compare the nutritional characteristics of four commercial and four local cultivars in order to better characterise the local ones and promote their use in animal nutrition. The seeds’ weight and the chemical composition, including starch and the energy, was evaluated. The in vitro fermentation characteristics were studied for 48 h using bull’s rumen fluid as inoculum. All the varieties showed the values’ weight corresponding to the specific botanical typology. The varieties significantly differed for protein, starch and lignin (p < 0.01) and structural carbohydrates (p < 0.05) concentration. No significant differences were observed for energy content. All the in vitro fermentation parameters resulted significantly different among the varieties. Organic matter degradability ranged between 89.9% and 85.1% and the potential gas production from 367 to 325 mL/g. The Pearson’s analysis showed significant correlation between morphological characteristics, chemical data and in vitro fermentation parameters. In conclusion, this investigation confirms the possibility of using local faba bean varieties (i.e., Aquino, Castrocielo, 13#5, 4#4) in ruminant nutrition with the advantage that, being local natural resources, they are better adapted to the climate and agronomic conditions and limit environmental impact.

scholarly journals Effect of phenological stage on nutrient composition, in vitro fermentation and gas production kinetics of Plantago lanceolata herbage

2018 ◽  
Vol 63 (No. 6) ◽  
pp. 251-260 ◽  
Author(s):  
K. Kara ◽  
S. Ozkaya ◽  
E. Baytok ◽  
BK Guclu ◽  
E. Aktug ◽  
...  
Keyword(s):  
Ph Value ◽  
Energy Content ◽  
Rumen Fluid ◽  
Plantago Lanceolata ◽  
Gas Production ◽  
Nutrient Composition ◽  
Metabolic Energy ◽  
Net Energy ◽  
Phenological Stages

This study was aimed at determining the nutrient composition and in vitro ruminal digestion values of Plantago lanceolata herbage in different phenological stages. The plant samples were gathered in the vegetative, flowering and early seed stages of the plant. The crude protein, diethyl ether extract, ash, non-fibre carbohydrates, and proanthocyanidins levels of the vegetative and flowering stages were higher than those of the early seed stage (P &lt; 0.001). Structural carbohydrate levels (P &lt; 0.05) were determined to have a higher value in the early seed stage. Glucose, fructose, Ca, K, Mg, P, Fe and Cu concentrations decreased as the plant matured (P &lt; 0.001), but Na, Zn, and Mn concentrations increased (P &lt; 0.05). The asymptote gas production, gas production rate (P &lt; 0.001), total gas production at 24 h (P = 0.002), metabolic energy, net energy lactation and organic matter digestibility values and the number of Entodinium (P &lt; 0.001) and total bacteria count (P = 0.026) of the flowering and vegetative stages were higher than those of the early seed stage. Methane produced by 0.2 g dry matter was similar in the three phenological stages (P = 0.078). The bound condensed tannins and saponin contents of plants and ammoniacal-N, number of total protozoa and pH value of rumen fluid were similar in the three different phenological stages (P &gt; 0.05). The present study indicates that P. lanceolata in the vegetative and flowering stages has, owing to its chemical composition, energy content and digestibility, the potential to be used as a forage source for ruminants in areas affected by drought.

The gas production capacity of purified chemicals and feedstuffs when incubated in vitro with rumen microbes as a possible indicator of energy availability in the rumen

1994 ◽  
Vol 1994 ◽  
pp. 91-91
Author(s):  
A.J. Chamberlain
Keyword(s):  
Microbial Activity ◽  
Energy Content ◽  
Production Capacity ◽  
Gas Production ◽  
Energy Availability ◽  
Rumen Microbes ◽  
Available Energy ◽  
Metabolisable Energy ◽  
Theoretical Considerations

The metabolisable protein system (AFRC, 1992) introduced the term Fermentable Metabolisable Energy (FME) as a measure of the amount of energy that was available in the rumen to support microbial activity. FME is currently derived from theoretical considerations rather than direct measurement; it is on based metabolisable energy (ME) but does not take into account rumen outflow rates and gives limited consideration to the chemical composition of feeds. Gas production from in vitro fermentation of feeds is an indicator of microbial activity which might be a suitable assessment of the rumen available energy content of feeds.

scholarly journals Effect of tannin from blackberry (Syzigium cumini) seed on in vitro rumen fermentation

2015 ◽  
Vol 25 ◽  
pp. 31-37
Author(s):  
SCK Barma ◽  
MR Amin ◽  
M Monirruzzaman ◽  
MB Sarker ◽  
AKMA Kabir
Keyword(s):  
Organic Matter ◽  
Energy Content ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Feed Additives ◽  
In Vitro Gas Production ◽  
Metabolisable Energy ◽  
Organic Matter Digestibility ◽  
Fermentation Parameters

An experiment was conducted to quantify the chemical composition, different forms of tannins and their effects on in vitro gas production, organic matter digestibility and metabolisable energy content in Syzigium cumini (Blackberry) seed with and without polyethelene glycol (PEG). The DM, OM, ash, CP, NDF and ADF contents of S. cumini seeds were 90.1, 87.6, 2.6, 4.6, 32.9 and 15.5%, respectively. The total phenol, total tannin, condensed tannin and hydrolysable tannin content were 5.89, 4.25, 0.42 and 0.05mg/g seed, respectively. In vitro gas production, organic matter digestibility and metabolisable energy content of S. cumini seed were 54 ml, 42.91% and 6.43 MJ/Kg DM, respectively. Addition of PEG to tannin containing seed was significantly (P<0.05) increased in vitro gas production, OMD and ME content. Higher levels of tannins in S. cumini seed could limit utilization through impaired digestibility and nutrient utilization but the addition of PEG increased all the fermentation parameters studied. It is predictable from the present findings that S. cumini seed could be utilized as natural source of feed additives to alter rumen fermentation parameters especially to protect protein and other nutrients from ruminal degradation, thereby make the nutrients available in the lower tract for higher milk and meat yield.Progress. Agric. 2014. 25: 31-37

scholarly journals Digestibility and rumen fermentation of a high forage diet pre-treated with a mixture of cellulase and xylanase enzymes

2021 ◽  
Vol 51 (3) ◽  
pp. 399-406
Author(s):  
K. Selzer ◽  
A. Hassen ◽  
A.M. Akanmu ◽  
A.Z.M. Salem
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Poor Quality ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Ruminal Fermentation ◽  
Forage Digestibility

Forages play an important role in ruminant animal production worldwide. Unlocking the nutritional potential of poor-quality tropical forages with fibrolytic enzymes would improve forage digestibility and utilization. Using in vitro and in vivo methods this study investigated the effect of pre-treating Smutsfinger hay for 24 hours with a mixture of fibrolytic enzyme (100% cellulase; 75% cellulase: 25% xylanase; 50% cellulase: 50% xylanase; 25% cellulase: 75% xylanase; 100% xylanase and a control with no enzyme) on ruminal fermentation and digestibility of nutrients by sheep. For in vitro fermentation, dry matter, neutral detergent fibre (NDF) degradability and volatile fatty acids (VFA) were determined with standard procedures. The same treatments were used for an in vivo digestibility trial using Merino sheep in a 6 x 6 Latin square design. Feed intake and total tract digestibility were recorded. Rumen fluid samples were collected daily, preserved, and analysed for VFA. The addition of 100% cellulase enzyme to Smutsfinger hay in vitro increased (P <0.05) NDF degradability and gas production compared with the control and inclusion of 100% xylanase enzyme. Both 100% cellulase and xylanase enzymes significantly reduced in vitro end time fermentation pH. A 50:50 mixture of cellulase and xylanase plus enzyme in vivo, increased acetate, total VFA concentration, and higher NDF and ADF digestibility of the test feed compared with the control. Inclusion of a 50-75% mixture of cellulase and 50-25% xylanase enzymes treatment led to higher gas production and butyrate concentration, decreased ruminal pH and improved nutrient digestibility.

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