Determination of Polyphenolic Content and In-vitro Antioxidant Capacity of the Leaves of Lagenaria siceraria (mol.) standl

2010 ◽  
Vol 2 (7) ◽  
pp. 162-169 ◽  
Author(s):  
R. Badmanaban ◽  
CN Patel ◽  
Vinay Patel
Keyword(s):  
Antioxidant Capacity ◽  
Lagenaria Siceraria ◽  
Polyphenolic Content

scholarly journals Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Anita Agnieszka Śliwińska ◽  
Katarzyna Sykłowska-Baranek ◽  
Anita Kośmider ◽  
Sebastian Granica ◽  
Karolina Miszczak ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Cytotoxic Activity ◽  
Phenolic Compound ◽  
Plant Extracts ◽  
Detrimental Effect ◽  
A549 Cells ◽  
Ferulic Acids

<p>In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of <em>Polyscias filicifolia</em> was developed. The phenolic compound content in <em>P. filicifolia</em> has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 µM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of <em>P. filicifolia</em> extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed.</p><p>The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 µM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 µM SA and 200 µM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.</p>

Methodological Aspects about Determination of Phenolic Compounds and In Vitro Evaluation of Antioxidant Capacity in the Honey: A Review

2009 ◽  
Vol 5 (4) ◽  
pp. 293-302 ◽  
Author(s):  
Jose Alvarez-Suarez ◽  
Sara Tulipani ◽  
Stefania Romandini ◽  
Alexis Vidal ◽  
Maurizio Battino
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
In Vitro Evaluation ◽  
Methodological Aspects

scholarly journals Determination of in Vitro Antioxidant Enzyme Capacity and Oxidative Stress Levels in Mazı Meşesi (Quercus infectoria)

2021 ◽  
Vol 9 (4) ◽  
pp. 814-817
Author(s):  
İlter Demirhan ◽  
Büşra Çitil ◽  
Mehmet Özyurt ◽  
Meltem Güngör ◽  
Erkan Öner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activities ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Quercus Infectoria

South East Anatolia Region has a large genetic plant diversity due to its physical and different climatic charesteristics. These plants are potential sources of antioxidants that prevent oxidative stress caused by oxygen and photons. In recent years, it has become important to study the antioxidant capacity of many molecules found naturally in foods and biological systems. The reason for this is that it is believed that when the consumption of food rich in antioxidants is increased, the risk of developing different degenerative diseases will be reduced. In this study, it was aimed to measure the antoxidant capacity of Quercus infectoria, G.olivier gal seeds grown in Southeastern Anatolia. Q. infectoria gal seeds from Sanlıurfa province were used in our study. Q. infectoria gal seeds were extracted with water, ethanol and methanol and then antioxidant enzyme activities (catalase and superoxide dismutase) and malondialdehyde levels, which are indicators of oxidative stress were determined by spectrophotometric methods. It was found that the antioxidant capacity (catalase and superoxide dismutase activities) of extracts obtained from ethanol and methanol were higher and their malondialdehyde levels were statistically lower than those obtained from water. However, it was determined that there was no statistically significant difference between the antioxidant capacity and malondialdehyde levels of the extracts obtained from methanol compared to the extracts obtained from ethanol. It has been concluded that Q. infectoria gal seed has a effective antioxidant effect. In addition, it was observed that extracts obtained from ethanol and methanol have higher antioxidant capacity than extracts obtained from water.

scholarly journals Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of Its Antioxidant Capacity in Vitro

2010 ◽  
Vol 11 (10) ◽  
pp. 3793-3802 ◽  
Author(s):  
Alejandra Hernández-Ceruelos ◽  
Eduardo Madrigal-Santillán ◽  
José Antonio Morales-González ◽  
Germán Chamorro-Cevallos ◽  
Martha Cassani-Galindo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Capacity ◽  
Antigenotoxic Effect

scholarly journals Phenolic Composition, Antioxidant Activity, andIn VitroAvailability of Four Different Berries

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Javier Marhuenda ◽  
María Dolores Alemán ◽  
Amadeo Gironés-Vilaplana ◽  
Alfonso Pérez ◽  
Gabriel Caravaca ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Total Phenolic Compounds ◽  
Anthocyanin Concentration

Polyphenols from berries have proved healthy effects after“in vitro”and“in vivo”studies, such as preventing tumor growing and neurodegenerative and cardiovascular diseases. We compared four different kinds of berries—strawberry, raspberry, blackberry, and blueberry—with the aim to distinguish their phenolic composition, concerning their antioxidant capacity along with their“in vitro”availability. Folin-Ciocalteu method was used for the determination of phenolic compounds, and the antioxidant capacity was measured by ORAC method. Moreover, the determination of anthocyanins was accomplished with an HPLC-DAD. Finally, we carried out an“in vitro”digestion to simulate the gastrointestinal digestion. All berries showed good antioxidant capacity with significant differences, besides high total phenolic compounds. Content of anthocyanins measured by HPLC-DAD varied between the different berries, namely, blackberries and strawberries which showed higher anthocyanin concentration. After“in vitro”digestion, berries showed poor bioavailability of the analysis of anthocyanins (9.9%–31.7%). Availability of total phenolic compounds was higher than anthocyanins (33%–73%). Moreover, strawberries and blackberries presented the less availability grade. Decrease in antioxidant activity measured by ORAC method was about 90% in all berries studied. Therefore, bioavailability of phenolic compounds remains unclear and more correlation between“in vitro”and“in vivo”studies seems to be necessary.

scholarly journals A novel procedure to measure the antioxidant capacity of Yerba maté extracts

2012 ◽  
Vol 32 (1) ◽  
pp. 126-133 ◽  
Author(s):  
Vanessa Graciela Hartwig ◽  
Luis Alberto Brumovsky ◽  
Raquel María Fretes ◽  
Lucila Sánchez Boado
Keyword(s):  
Antioxidant Capacity ◽  
Dry Matter ◽  
Absolute Methanol ◽  
Yerba Mate ◽  
Dpph Assay ◽  
Dicaffeoylquinic Acid ◽  
Polyphenolic Content ◽  
Pure Compounds ◽  
Total Polyphenolic Content

Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 µL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 µM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay.

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