scholarly journals A novel procedure to measure the antioxidant capacity of Yerba maté extracts

2012 ◽  
Vol 32 (1) ◽  
pp. 126-133 ◽  
Author(s):  
Vanessa Graciela Hartwig ◽  
Luis Alberto Brumovsky ◽  
Raquel María Fretes ◽  
Lucila Sánchez Boado
Keyword(s):  
Antioxidant Capacity ◽  
Dry Matter ◽  
Absolute Methanol ◽  
Yerba Mate ◽  
Dpph Assay ◽  
Dicaffeoylquinic Acid ◽  
Polyphenolic Content ◽  
Pure Compounds ◽  
Total Polyphenolic Content

Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 µL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 µM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay.

scholarly journals IN VITRO ANTIOXIDANT CAPACITY, PHENOLIC, ASCORBIC ACID AND LYCOPENE CONTENT OF GUAVA (Psidium guajava L.) JUICES AND NECTARS

2009 ◽  
Vol 27 (2) ◽  
Author(s):  
CARLOS ALEXANDRE KOGUISHI DE BRITO ◽  
PRISCILA BECKER SIQUEIRA ◽  
JANE CRISTINA DE SOUZA ◽  
HELENA MARIA ANDRÉ BOLINI
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Ascorbic Acid Content ◽  
Psidium Guajava ◽  
Polyphenolic Compounds ◽  
Polyphenolic Content ◽  
Total Polyphenolic Content ◽  
Lycopene Content ◽  
Free Radicals Scavenging

capacity in vitro of three different brands of guava nectars and juices, through free radicals scavenging methods, 1,1-diphenyl-2-picrylhydrazine (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS.+), and its correlation with the total polyphenolic content, total lycopene and ascorbic acid. Brands revealed statistical differences (p£ 0.05) in antioxidant capacity, ranging from 1.9 to 7.7 molTE/mL. Antioxidant capacity presented positive correlation for ascorbic acid content and polyphenolic compounds, being relatively low for the lycopene. Different process can influence the content of these compounds as well as interfere in their antioxidant capacity. The control of the production process is important to add value to guava products and fulfill the new tendency of the market.

scholarly journals Anti-austeric Activity of Phenolic Constituents of Seeds of Arctium lappa

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yasuhiro Tezuka ◽  
Keiichi Yamamoto ◽  
Suresh Awale ◽  
Feng Li ◽  
Satoshi Yomoda ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cytotoxic Activity ◽  
Chlorogenic Acid ◽  
Human Pancreatic Cancer ◽  
Arctium Lappa ◽  
Dicaffeoylquinic Acid ◽  
Phenolic Constituents ◽  
Pure Compounds ◽  
F 14

From seeds of Arctium lappa L. (Asteraceae) we obtained arctigenin (1), arctiin (2), chlorogenic acid (3), 4,5-dicaffeoylquinic acid (4), 3,5-dicaffeoylquinic acid (5), 3,4-dicaffeoylquinic acid (6), matairesinol (11), isolappaol A (12), lappaol F (14), and lappaol B (15), together with 1:1 mixtures of isolappaol C (7) and lappaol C (8), arctignan E (9) and arctignan D (10), and 12 and lappaol A (13), while 3,3′,4′-tri- O-demethylarctigenin (16), 3,3′-di- O-demethyl-4′-dehydroxyarctigenin (17), and 3- O-demethylarctigenin (18) were obtained by anaerobic microbiological metabolism of 1. Then, we evaluated the in vitro preferential cytotoxic activity of these pure compounds and 1:1 mixtures, together with enterodiol (19) and enterolactone (20), against human pancreatic cancer PANC-1 cells in nutrient-deprived medium (NDM). Among them, 1 and 18 showed potent activity, with PC50 values of 1.75 and 4.38 μ M, respectively, while 11, 15, and 17 showed mild activity with PC50 values of 31.1, 30.9, and 38.7 μ/M, respectively. By comparing their structures and PC50 values, the following structural moieties could be concluded to be important for the preferential cytotoxicity of 1: 1) the 3-hydroxy-4-methoxyphenyl group at the 2-position on the γ-butyrolactone ring, 2) the less polar substituent at the 3-position on the γ-butyrolactone ring, and 3) the γ-butyrolactone ring.

scholarly journals In Vitro Anti-inflammatory and Antioxidant Potential of Si-Miao-San, its Modifications and Pure Compounds

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800
Author(s):  
Agnieszka D. Lower-Nedza ◽  
Carmen Kuess ◽  
Haiyu Zhao ◽  
Baolin Bian ◽  
Adelheid H. Brantner
Keyword(s):  
Cell Damage ◽  
Natural Antioxidants ◽  
Dpph Assay ◽  
Pure Compounds ◽  
Tyrosinase Inhibitors ◽  
Anti Inflammatory ◽  
Inhibitory Potential ◽  
The Ancient Chinese ◽  
Low Activity

The ancient Chinese prescription Si-Miao-San (SMS), which is widely used for the treatment of various diseases, e.g. rheumatic disorders, has been modified (m1SMS, m2SMS). The purpose of this study was to investigate the anti-inflammatory, antioxidant, and anti-tyrosinase effects of Si-Miao-San, of its two modifications, the component herbs, and its main pure ingredients. In vitro tyrosinase, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and lipid peroxidation (LPO) assays were carried out in order to determine the inhibitory potential of the samples. The traditionally applied decoctions as well as their fractions ( n-hexane, DCM, n-BuOH) were tested for their activities in concentrations of 100 μg/mL and 400 μg/mL, and the pure compounds in a range 6.25 μg/mL to 100 μg/mL. In conclusion, the decoction of m2SMS exhibited strong antioxidant activity in the DPPH assay, while the decoction of the classical SMS formulation showed low activity. The present results have shown the modifications to be more efficient scavengers of free radicals, such as superoxide and peroxide radicals. In addition, the decoctions of the two modifications have been shown to be more potent tyrosinase inhibitors. These formulas may thus be used as anti-inflammatory and anti-aging prescriptions, as they may help to prevent cell damage. This study clearly establishes the two modifications of Si-Miao-San as valuable sources of natural antioxidants and anti-inflammatory agents, and also as candidates in the search for modern pharmaceuticals.

scholarly journals Constituents of Cuscuto Reflexa are anti-HIV Agents

1997 ◽  
Vol 8 (1) ◽  
pp. 70-74 ◽  
Author(s):  
N Mahmood ◽  
S Piacente ◽  
A Burke ◽  
Al Khan ◽  
C Pizza
Keyword(s):  
Crude Extract ◽  
Quinic Acid ◽  
Cuscuta Reflexa ◽  
Gp 120 ◽  
Dicaffeoylquinic Acid ◽  
Pure Compounds ◽  
Specific Proteins ◽  
Anti Hiv Agents ◽  
Anti Hiv

Crude water extracts of Cuscuta reflexa exhibited anti-HIV activity. Fractionation of the crude extract led to the isolation of nine pure compounds with closely related structures, showing interesting structure activity relationships. 3,5,7,4′-Tetrahydroxyflavanone (aromadendrin) inhibited infection by binding to V3 loop of gp 120 and inhibiting its interaction with CD4, whereas 3,5,7,3′,4′-pentahydroxyflavanone (taxifolin), with an extra OH group in the 3′ position in ring B was less specific and exhibited less selectivity in cell cultures. In general, flavanones containing an extra OH group in the 3′ position (taxifolin, taxifolin-7-O-β-D-glucopyranoside and coccinoside B) were less specific and inhibited viral protease, reverse transcriptase, CD4 /gp120 interaction in vitro and bound to non specific proteins. Other compounds isolated from C reflexa were derivatives of quinic acids; 3,4-O-dicaffeoylquinic acid was more active than 3-O-caffeoyl quinic acid. The anti-HIV activity of crude extract may be the result of combinatory effects with compounds of different modes of action.

Determination of the Antioxidant Capacity of JiangHuang JiangZhi Capsule with DPPH Assay in Vitro

2011 ◽  
Vol 236-238 ◽  
pp. 913-916
Author(s):  
Sheng Zhang ◽  
Xiang Zhou Li ◽  
Chun Tao Kuang
Keyword(s):  
Free Radicals ◽  
Antioxidant Capacity ◽  
Antioxidant Effect ◽  
Assay Method ◽  
Dpph Assay

To investigate the antioxidant effect in vitro of JiangHuang JiangZhi capsule, different concentrations of content of capsule were determinated by DPPH assay method. Results showed Jianghuang Jiangzhi capsule had antioxidant capacity on eliminating DPPH free radicals.

scholarly journals Antioxidant capacity and total polyphenolic content in quince (Cydonia oblonga Mill.) fruit

2013 ◽  
Vol 19 (3-4) ◽  
Author(s):  
N. Papp ◽  
T. Szabó ◽  
Z. Szabó ◽  
J. Nyéki ◽  
A. Hegedűs
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Cydonia Oblonga ◽  
Antioxidant Power ◽  
Commercial Cultivars ◽  
Polyphenolic Content ◽  
Ferric Reducing Antioxidant Power ◽  
Total Polyphenolic Content

Fruits of twelve quince (Cydonia oblonga Mill.) commercial cultivars and selections were compared in the ferric reducing antioxidant power (FRAP) and total phenolic content (TPC) of intact and peeled fruits. The antioxidant capacity and total phenolic content ranged between 5.99 and 63.10 mmol AA/100 g FW, and 3.92 and 12.83 g GA/100 g FW, respectively. These ranges cover an almost 8-times variation among the tested genotypes in case of antioxidant capacity and also declares two-times variation for total polyphenolic content. Cultivars ‘Aromate’ and ‘Bereczki’ possessed the lowest antioxidant capacity and ‘Román portugál’ and ‘Bereczki’ had the lowest polyphenolic contents. In contrast, cultivars ‘Champion’ and ‘Konstantinápolyi’ possessed the highest antioxidant capacity and ‘Mezôtúri’ and ‘De Husi’ had the highest polyphenolic contents. The Pearson’s coeffi cient was relatively moderate but signifi cant (r=0.51) indicating a weak interdependence between FRAP and TPC values of quince fruits. Our results led us to the conclusion that quince might be considered as a valuable source of antioxidants and polyphenolics.

scholarly journals Description of the Guelder Rose Fruit in Terms of Chemical Composition, Antioxidant Capacity and Phenolic Compounds

2021 ◽  
Vol 11 (19) ◽  
pp. 9221
Author(s):  
Monika Mazur ◽  
Jakub Szperlik ◽  
Anna Marietta Salejda ◽  
Grażyna Krasnowska ◽  
Joanna Kolniak-Ostek ◽  
...  
Keyword(s):  
Food Production ◽  
Dry Matter ◽  
Titratable Acidity ◽  
Biologically Active ◽  
Binary Solvent ◽  
Tatra Mountains ◽  
Viburnum Opulus ◽  
Polyphenolic Content ◽  
Total Polyphenolic Content ◽  
The Tatra Mountains

The Viburnum opulus (guelder rose) fruit is a Polish native fruit that grows across almost the entire territory of the country, except for the Tatra Mountains. The fruits are rich in nutrients and biologically active compounds, however, they are rarely used for food production or as a dietary ingredient. This study assessed this fruit for dry matter (17.3% ± 0.7), extract (13% ± 0.53), titratable acidity (1.78 g/100 g ± 0.48), pectin (1.10% ± 0.97), vitamin C (47.79 mg/100 g ± 1.02), the amount of sugar identified as fructose (6.85 g/100 g ± 0.67), antioxidant activity, ABTS (14.64 ± 0.61 μM Trolox/100 g), FRAP (211.14 ± 1.04 µM Trolox/100 g) and total polyphenolic content TPC (1695.88 ± 0.86 mg GAE/100 g). The analysis of phenolic identification was performed using the ACQUITY Ultra Performance LC (UPLC) system with the binary solvent manager and PDA detector.

scholarly journals THE TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT PROPERTIES OF VARIOUS HONEY AND SUGARS

2018 ◽  
Vol 11 (5) ◽  
pp. 467
Author(s):  
Chaiyavat Chaiyasut ◽  
Periyanaina Kesika ◽  
Sartjin Peerajan ◽  
Bhagavathi Sundaram Sivamaruthi
Keyword(s):  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Polyphenol Content ◽  
Polyphenolic Content ◽  
Total Polyphenolic Content ◽  
Gallic Acid Equivalent

Objective: Honey and sugars are rich in polyphenols and known for several health benefits. The quality of the honey varied on the floral source and geographical region. The sugar quality depends on the processing and source. The study aimed to evaluate the total polyphenolic content and antioxidant properties of selected Thai honey and sugar samples.Methods: Total polyphenol content and antioxidant properties were determined by the colorimetric method, and 2, 2′-azino-bis-3-ethylbenzthiazoline- 6-sulfonic acid, ferric reducing antioxidant potential (FRAP), and ferrous ion-chelating assays, respectively.Results: The honey from Eupatorium odoratum Linn (HEO) (0.75 mg gallic acid equivalent/ml of sample), and meal cane sugar (MCS) (4.13 mg gallic acid equivalent/g of sample) exhibited higher polyphenol content among the honey, and sugar samples, respectively. The refined sugar (RS) showed least phenolic content (0.02 mg gallic acid equivalent/g of sample) when compared to all tested sugars and honey samples. HEO exhibited high Trolox equivalent antioxidant capacity (TEAC; 235.93 mg TEAC per ml sample) among the honey samples. Among the sugars, MCS showed high TEAC (3891.22 mg TEAC per g sample). The honey from Nephelium lappaceum Linn and HEO has superior FRAP value and chelating power, respectively. The RS was least in total antioxidant capacity, FRAP, and chelating power.Conclusion: The HEO, and MCS and palm sugars are harbored with phenolic content and antioxidants. The refining process drastically diminished the quality of the sugar. The information may help to choose best carbon source for the preparation of fermented beverages with improved functional properties.

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