Spatial and temporal population dynamics of a phyllosphere colonizing Bacillus subtilis biological control agent of sugar beet cercospora leaf spot

Postharvest fungal disease is one of the significant factors that limits the storage period and marketing life of peaches, and even result in serious economic losses worldwide. Biological control using microbial antagonists has been explored as an alternative approach for the management of postharvest disease of fruits. However, there is little information available regarding to the identification the fungal pathogen species that cause the postharvest peach diseases and the potential and mechanisms of using the Bacillus subtilis JK-14 to control postharvest peach diseases. In the present study, a total of six fungal isolates were isolated from peach fruits, and the isolates of Alternaria tenuis and Botrytis cinerea exhibited the highest pathogenicity and virulence on the host of mature peaches. In the culture plates, the strain of B. subtilis JK-14 showed the significant antagonistic activity against the growth of A. tenuis and B. cinerea with the inhibitory rates of 81.32% and 83.45% at 5 days after incubation, respectively. Peach fruits treated with different formulations of B. subtilis JK-14 significantly reduced the mean disease incidences and lesion diameters of A. tenuis and B. cinerea. The greatest mean percent reduction of the disease incidences (81.99% and 71.34%) and lesion diameters (82.80% and 73.57%) of A. tenuis and B. cinerea were obtained at the concentration of 1 × 107 CFU mL−1 (colony forming unit, CFU). Treatment with the strain of B. subtilis JK-14 effectively enhanced the activity of the antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in A. tenuis and B. cinerea inoculated peach fruits. As such, the average activities of SOD, POD and CAT were increased by 36.56%, 17.63% and 20.35%, respectively, compared to the sterile water treatment. Our results indicate that the isolates of A. tenuis and B. cinerea are the main pathogens that cause the postharvest peach diseases, and the strain of B. subtilis JK-14 can be considered as an environmentally-safe biological control agent for the management of postharvest fruits diseases. We propose the possible mechanisms of the strain of B. subtilis JK-14 in controlling of postharvest peach diseases.

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POPULATION DYNAMICS OF ERWINIA AMYLOVORA AND A BIOLOGICAL CONTROL AGENT, ERWINIA HERBICOLA, ON APPLE BLOSSOM PARTS

Acta Horticulturae ◽

10.17660/actahortic.1987.217.37 ◽

1987 ◽

pp. 221-222 ◽

Cited By ~ 8

Author(s):

J.R. Rundle ◽

S.V. Beer

Keyword(s):

Biological Control ◽

Population Dynamics ◽

Erwinia Amylovora ◽

Biological Control Agent ◽

Control Agent ◽

Erwinia Herbicola

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Evaluation of Bacillus subtilis C186 as a potential biological control of sapstain and mould on unseasoned lumber

Canadian Journal of Microbiology ◽

10.1139/m87-192 ◽

1987 ◽

Vol 33 (12) ◽

pp. 1102-1107 ◽

Cited By ~ 23

Author(s):

Keith A. Seifert ◽

Wendy E. Hamilton ◽

Colette Breuil ◽

Maureen Best

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Biological Control Agent ◽

Control Agent ◽

Mould Growth

Bacillus subtilis C186 was evaluated as a potential biological control agent for sapstain and mould growth on unseasoned lumber. The strain produces antibiotics that are fungistatic to many pine-inhabiting fungi. Although pine blocks precolonized with C186 and subsequently inoculated with staining fungi were generally less discoloured than blocks inoculated only with fungi, discolouration was not sufficiently prevented to warrant further consideration of this strain as a biological control agent. The disappointing performance of C186 is traced to its poor colonization of wood.

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Efficacy of Bacillus subtilis V26 as a biological control agent against Rhizoctonia solani on potato

Comptes Rendus Biologies ◽

10.1016/j.crvi.2015.09.005 ◽

2015 ◽

Vol 338 (12) ◽

pp. 784-792 ◽

Cited By ~ 16

Author(s):

Saoussen Ben Khedher ◽

Olfa Kilani-Feki ◽

Mouna Dammak ◽

Hayfa Jabnoun-Khiareddine ◽

Mejda Daami-Remadi ◽

...

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Rhizoctonia Solani ◽

Biological Control Agent ◽

Control Agent

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A process-based population dynamics model to explore target and non-target impacts of a biological control agent

Ecological Modelling ◽

10.1016/j.ecolmodel.2009.04.039 ◽

2009 ◽

Vol 220 (17) ◽

pp. 2035-2050 ◽

Cited By ~ 18

Author(s):

Darren J. Kriticos ◽

Michael S. Watt ◽

Toni M. Withers ◽

Agathe Leriche ◽

Michelle C. Watson

Keyword(s):

Biological Control ◽

Population Dynamics ◽

Biological Control Agent ◽

Control Agent ◽

Dynamics Model ◽

Population Dynamics Model

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AKTIVITAS SIDEROFOR BACILLUS SUBTILIS SEBAGAI PEMACU PERTUMBUHAN DAN PENGENDALI PATOGEN TANAMAN TERUNG

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.217170-178 ◽

2017 ◽

Vol 17 (2) ◽

pp. 170 ◽

Cited By ~ 1

Author(s):

Nur Prihatiningsih ◽

Heru Adi Djatmiko ◽

Puji Lestari

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Plant Growth ◽

Biological Control Agent ◽

Control Agent ◽

Leaf Number ◽

Growth Promoters ◽

Root Volume ◽

Volume Weight ◽

Plant Growth Promoters

Siderophore activity of Bacillus subtilis as plant growth promoters and biological control agent of eggplants pathogens. The aims of this research were to identify the siderophores of B. subtilis, to assess its activities as plant growth promoters and biological control agent of eggplants pathogens. Five isolates of B. subtilis i.e.B46, B209, B211, B298 and B 315 grown on SDCASA medium. The isolate which showed the best siderophores production was then further studied on its ability as a growth promoter on eggplants in two soil types with different Fe content. The inhibitory test was conducted against two kinds of pathogens, namely Colletotrichum sp. and Ralstonia solanacearum. The greenhouse experiment was arranged using a factorial completely randomized block design. The first factor was the B. subtilis (B. subtilis B298 and without B. subtilis B298), second factor was the type of soil (Ultisol and Andisol). The variables measured were Fe uptake by plants, plant growth parameters on eggplant i.e. height, leaf number, root length, root volume, weight of fresh and dried shoot as well as fresh and dry root, percentage of inhibition to fungal and bacterial eggplant pathogens. The results showed that the five isolates of B. subtilis were able to produce siderophores as catecholate and hydroxamate types. The best siderophore production was showed by B. subtilis B298. The ability of B. subtilis B298 in accelerating the growth of plants was indicated by the increased of uptake Fe, plant height, leaf number, root volume, weight of dried plants by 45.62%, 25.48%, 19.45%, 41.10% and 34.89% respectively. The inhibition to the fungal and bacterial eggplant pathogens best shown by the isolates of B. subtilis B298 with 55.4% and 22 mm respectively.

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Population Dynamics of Broadleaf Weeds in Turfgrass as Influenced by Chemical and Biological Control Methods

Weed Science ◽

10.1614/ws-06-144 ◽

2007 ◽

Vol 55 (4) ◽

pp. 371-380 ◽

Cited By ~ 8

Author(s):

Mohammed H. Abu-Dieyeh ◽

Alan K. Watson

Keyword(s):

Biological Control ◽

Population Dynamics ◽

Weed Control ◽

Seed Bank ◽

Biological Control Agent ◽

Control Agent ◽

Weed Species ◽

Early Autumn ◽

Herbicide Treatment ◽

Broadleaf Species

A 3-yr field study was conducted to determine the effect of a biological control agent,Sclerotinia minorJagger, and a common herbicide, Killex, on the population dynamics of dandelion and other broadleaf species and on the dandelion seed bank. Treatments were applied as one spring, one early autumn, or a spring plus an early autumn treatment per year. The response of the dandelion population to a spring herbicide treatment was similar to two applications per year (spring and early autumn). Significantly less dandelion control occurred after the first early autumn application of the herbicide. Two weeks after application, spring or early autumn treatments withS. minorwere equally effective in suppressing dandelions. In the second year of the two applications per year ofS. minortreatment, weed control was equivalent to the herbicide. By the third year of the one spring application ofS. minor, weed control was equivalent to the herbicide. Generally over the study period, the early autumn application ofS. minorwas less effective than the spring or the spring and early autumn applications. TheS. minortreatments significantly reduced the dandelion seed bank, and this effect was not significantly different from the Killex herbicide treatment. The rate, frequency, and seasonal timing of application had no effect on the dandelion seed-bank size, but terminating the application would gradually replenish the seed bank. Populations of white clover, broadleaf plantain, birdsfoot trefoil, and common ragweed were similarly suppressed by either theS. minoror the herbicide treatments. Yellow woodsorrel significantly increased after 1 yr of herbicide treatment compared with theS. minorand untreated control treatments, indicating a possible weed species shift. Turf quality was improved because of the herbicide andS. minortreatments, but grass injury and smooth crabgrass invasion were recorded in 17% of herbicide-treated plots.

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