Efficacy of Bacillus subtilis V26 as a biological control agent against Rhizoctonia solani on potato

Postharvest fungal disease is one of the significant factors that limits the storage period and marketing life of peaches, and even result in serious economic losses worldwide. Biological control using microbial antagonists has been explored as an alternative approach for the management of postharvest disease of fruits. However, there is little information available regarding to the identification the fungal pathogen species that cause the postharvest peach diseases and the potential and mechanisms of using the Bacillus subtilis JK-14 to control postharvest peach diseases. In the present study, a total of six fungal isolates were isolated from peach fruits, and the isolates of Alternaria tenuis and Botrytis cinerea exhibited the highest pathogenicity and virulence on the host of mature peaches. In the culture plates, the strain of B. subtilis JK-14 showed the significant antagonistic activity against the growth of A. tenuis and B. cinerea with the inhibitory rates of 81.32% and 83.45% at 5 days after incubation, respectively. Peach fruits treated with different formulations of B. subtilis JK-14 significantly reduced the mean disease incidences and lesion diameters of A. tenuis and B. cinerea. The greatest mean percent reduction of the disease incidences (81.99% and 71.34%) and lesion diameters (82.80% and 73.57%) of A. tenuis and B. cinerea were obtained at the concentration of 1 × 107 CFU mL−1 (colony forming unit, CFU). Treatment with the strain of B. subtilis JK-14 effectively enhanced the activity of the antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in A. tenuis and B. cinerea inoculated peach fruits. As such, the average activities of SOD, POD and CAT were increased by 36.56%, 17.63% and 20.35%, respectively, compared to the sterile water treatment. Our results indicate that the isolates of A. tenuis and B. cinerea are the main pathogens that cause the postharvest peach diseases, and the strain of B. subtilis JK-14 can be considered as an environmentally-safe biological control agent for the management of postharvest fruits diseases. We propose the possible mechanisms of the strain of B. subtilis JK-14 in controlling of postharvest peach diseases.

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Evaluation of Bacillus subtilis C186 as a potential biological control of sapstain and mould on unseasoned lumber

Canadian Journal of Microbiology ◽

10.1139/m87-192 ◽

1987 ◽

Vol 33 (12) ◽

pp. 1102-1107 ◽

Cited By ~ 23

Author(s):

Keith A. Seifert ◽

Wendy E. Hamilton ◽

Colette Breuil ◽

Maureen Best

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Biological Control Agent ◽

Control Agent ◽

Mould Growth

Bacillus subtilis C186 was evaluated as a potential biological control agent for sapstain and mould growth on unseasoned lumber. The strain produces antibiotics that are fungistatic to many pine-inhabiting fungi. Although pine blocks precolonized with C186 and subsequently inoculated with staining fungi were generally less discoloured than blocks inoculated only with fungi, discolouration was not sufficiently prevented to warrant further consideration of this strain as a biological control agent. The disappointing performance of C186 is traced to its poor colonization of wood.

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Laetisaria arvalis (Aphyllophorales, Corticiaceae): A Possible Biological Control Agent for Rhizoctonia solani and Pythium Species

Mycologia ◽

10.2307/3759765 ◽

1980 ◽

Vol 72 (4) ◽

pp. 728 ◽

Cited By ~ 21

Author(s):

H. H. Burdsall ◽

H. C. Hoch ◽

M. G. Boosalis ◽

E. C. Setliff

Keyword(s):

Biological Control ◽

Rhizoctonia Solani ◽

Biological Control Agent ◽

Control Agent ◽

Pythium Species ◽

Laetisaria Arvalis

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AKTIVITAS SIDEROFOR BACILLUS SUBTILIS SEBAGAI PEMACU PERTUMBUHAN DAN PENGENDALI PATOGEN TANAMAN TERUNG

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.217170-178 ◽

2017 ◽

Vol 17 (2) ◽

pp. 170 ◽

Cited By ~ 1

Author(s):

Nur Prihatiningsih ◽

Heru Adi Djatmiko ◽

Puji Lestari

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Plant Growth ◽

Biological Control Agent ◽

Control Agent ◽

Leaf Number ◽

Growth Promoters ◽

Root Volume ◽

Volume Weight ◽

Plant Growth Promoters

Siderophore activity of Bacillus subtilis as plant growth promoters and biological control agent of eggplants pathogens. The aims of this research were to identify the siderophores of B. subtilis, to assess its activities as plant growth promoters and biological control agent of eggplants pathogens. Five isolates of B. subtilis i.e.B46, B209, B211, B298 and B 315 grown on SDCASA medium. The isolate which showed the best siderophores production was then further studied on its ability as a growth promoter on eggplants in two soil types with different Fe content. The inhibitory test was conducted against two kinds of pathogens, namely Colletotrichum sp. and Ralstonia solanacearum. The greenhouse experiment was arranged using a factorial completely randomized block design. The first factor was the B. subtilis (B. subtilis B298 and without B. subtilis B298), second factor was the type of soil (Ultisol and Andisol). The variables measured were Fe uptake by plants, plant growth parameters on eggplant i.e. height, leaf number, root length, root volume, weight of fresh and dried shoot as well as fresh and dry root, percentage of inhibition to fungal and bacterial eggplant pathogens. The results showed that the five isolates of B. subtilis were able to produce siderophores as catecholate and hydroxamate types. The best siderophore production was showed by B. subtilis B298. The ability of B. subtilis B298 in accelerating the growth of plants was indicated by the increased of uptake Fe, plant height, leaf number, root volume, weight of dried plants by 45.62%, 25.48%, 19.45%, 41.10% and 34.89% respectively. The inhibition to the fungal and bacterial eggplant pathogens best shown by the isolates of B. subtilis B298 with 55.4% and 22 mm respectively.

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Bacillus subtilis IAB/BS03 as a potential biological control agent

European Journal of Plant Pathology ◽

10.1007/s10658-016-0945-3 ◽

2016 ◽

Vol 146 (3) ◽

pp. 597-608 ◽

Cited By ~ 15

Author(s):

Estefanía Hinarejos ◽

Mayte Castellano ◽

Ismael Rodrigo ◽

José María Bellés ◽

Vicente Conejero ◽

...

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Biological Control Agent ◽

Control Agent

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Bacillus subtilis TE3: A promising biological control agent against Bipolaris sorokiniana, the causal agent of spot blotch in wheat (Triticum turgidum L. subsp. durum)

Biological Control ◽

10.1016/j.biocontrol.2019.02.012 ◽

2019 ◽

Vol 132 ◽

pp. 135-143 ◽

Cited By ~ 9

Author(s):

Eber Villa-Rodríguez ◽

Fannie Parra-Cota ◽

Ernestina Castro-Longoria ◽

Jaime López-Cervantes ◽

Sergio de los Santos-Villalobos

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Triticum Turgidum ◽

Biological Control Agent ◽

Bipolaris Sorokiniana ◽

Spot Blotch ◽

Control Agent ◽

Causal Agent

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Draft Genome Sequence of Bacillus subtilis ALBA01, a Strain with Antagonistic Activity against the Soilborne Fungal Pathogen of Onion Setophoma terrestris

Genome Announcements ◽

10.1128/genomea.00455-16 ◽

2016 ◽

Vol 4 (3) ◽

Cited By ~ 4

Author(s):

Andrea G. Albarracín Orio ◽

Romina A. Tobares ◽

Daniel A. Ducasse ◽

Andrea M. Smania

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Biological Activity ◽

Genome Sequence ◽

Fungal Pathogen ◽

Biological Control Agent ◽

Draft Genome ◽

Control Agent ◽

Antagonistic Activity ◽

Setophoma Terrestris

Bacillus subtilis is a nonpathogenic bacterium that lives in soil and has long been used as biological control agent in agriculture. Here, we report the genome sequence of a B. subtilis strain isolated from rhizosphere of onion that shows strong biological activity against the soilborne fungal pathogen Setophoma terrestris .

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Uji Antagonisme Bacillus cereus terhadap Rhizoctonia solani dan Sclerotium rolfsii

Buletin Tanaman Tembakau Serat & Minyak Industri ◽

10.21082/bultas.v7n1.2015.1-8 ◽

2016 ◽

Vol 7 (1) ◽

pp. 1

Author(s):

Nurul Hidayah ◽

Titiek Yulianti

Keyword(s):

Biological Control ◽

Growth Rate ◽

Rhizoctonia Solani ◽

Biological Control Agent ◽

Sclerotium Rolfsii ◽

Control Agent ◽

Dual Culture ◽

Bacillus Sp ◽

Unfavorable Condition

Jamur Rhizoctonia solani dan Sclerotium rolfsii merupakan kelompok jamur steril (tidak menghasilkan spora) tetapi dapat menghasilkan sklerosia sebagai sumber inokulum primer, dan struktur istirahat jamur yang dapat bertahan selama beberapa tahun di dalam tanah saat kondisi lingkungan kurang menguntungkan. Penggunaan fungisida, fumigasi, dan solarisasi tanah telah digunakan untuk mengendalikan kedua jamur tersebut, namun hasil yang diperoleh masih beragam. Pengendalian hayati dengan menggunakan bakteri Bacillus sp. yang merupakan salah satu kelompok agens hayati patogen diketahui memberikan hasil yang baik pada beberapa tanaman. Penelitian yang bertujuan menguji potensi B. cereus dalam menghambat pertumbuhan jamur R. solani dan S. rolfsii secara in vitro dilaksanakan di Laboratorium Fitopatologi Balittas dengan menggunakan metode dual culture pada media potato dextrose agar (PDA). Miselia jamur R. solani dan S. rolfsii masing-masing berumur 5 hari diambil dengan menggunakan cork borer ukuran 0,5 cm ditanam pada media PDA berhadapan dengan B. cereus dengan jarak 3 cm. Penelitian disusun dalam rancangan acak lengkap dan diulang empat kali. Pengamatan dilakukan terhadap persentase penghambatan pertumbuhan jamur oleh Bacillus sp. dan laju pertumbuhan jamur. Hasil penelitian menunjukkan bahwa Bacillus sp. mampu menghambat pertumbuhan miselia R. solani dan S. rolfsii masing-masing sebesar 68,9% dan 33% pada hari ketiga setelah perlakuan. Keberadaan B. cereus dapat memperlambat laju pertumbuhan R. solani (15,5 mm/24 jam), dibandingkan perlakuan kontrol (tanpa B. cereus) sebesar 19,7 mm/24 jam. Hasil ini menunjukkan bahwa B. cereus dapat menghambat pertumbuhan R. solani dan berpotensi untuk dikembangkan sebagai agens hayati. Rhizoctonia solani and Sclerotium rolfsii (the causal agents of damping off disease on various hosts) are the group of sterile fungi that cannot produce spores. Nevertheless, they produce sclerotia as primary inocula and resting spores when facing unfavorable condition. Several control methods using chemical fungicides and solarization had been conducted, but the results were still inconsistent. In addition, the use of Bacillus sp. as a biological control agent for several plant diseases had provided successful results. Furthermore, the research aimed to evaluate the potency of B. cereus towards R. solani and S. rolfsii in vitro was carried out in the laboratory of phytopathology using dual culture method on PDA medium. Five days of R. solani and S. rolfsii miselia were plugged and inoculated on PDA medium toward B. cereus. The research was arranged by completely randomized design with four replicates. The percentage of fungal inhibition and fungal growth rate were observed. The result showed that B. cereus exhibited mycelial growth inhibition activity of R. solani and S. rolfsii by 68,9% and 33% three days after treatments, respectively. The result also indicated that B. cereus has a potential prospect to be developed as a biological control agent because the bacteria could suspend the growth rate of R. solani.

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