PVI-16 Performance evaluation of VIDIA® HBs AG, a new automated immunoassay test for the qualitative detection of HBs antigen in human serum and plasma samples

2009 ◽  
Vol 46 ◽  
pp. S34
Author(s):  
V. Barlet ◽  
R. Croisé ◽  
M. Maniez-Montreuil ◽  
F. Descamps ◽  
J.C. Plantier ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Human Serum ◽  
Plasma Samples ◽  
Hbs Antigen ◽  
Qualitative Detection ◽  
Automated Immunoassay ◽  
Hbs Ag

scholarly journals Immunochromatographic Strip Test for Rapid Detection of Nevirapine in Plasma Samples from Human Immunodeficiency Virus-Infected Patients

2007 ◽  
Vol 51 (9) ◽  
pp. 3361-3363 ◽  
Author(s):  
Tim R. Cressey ◽  
Sawitree Nangola ◽  
Yardpiroon Tawon ◽  
Mookda Pattarawarapan ◽  
Marc Lallemant ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Limit Of Detection ◽  
Drug Level ◽  
Plasma Samples ◽  
Immunochromatographic Strip ◽  
Resource Limited ◽  
Qualitative Detection ◽  
Immunodeficiency Virus ◽  
Immunochromatographic Strip Test ◽  
Strip Test

ABSTRACT We report a novel one-step immunochromatographic strip test for the rapid, qualitative detection of nevirapine in plasma samples from human immunodeficiency virus-infected patients. The sensitivity was 100% (95% confidence interval [95% CI], 97.8 to 100%), and the specificity was 99.5% (95% CI, 97.2 to 99.9%). The limit of detection was 25 ng/ml. Immunochromatographic strip tests are simple, rapid, and cheap assays that could greatly facilitate drug level monitoring in resource-limited settings.

scholarly journals Dihydro-sphingosine 1-phosphate interacts with carrier proteins in a manner distinct from that of sphingosine 1-phosphate

2018 ◽  
Vol 38 (5) ◽  
Author(s):  
Yuko Mishima ◽  
Makoto Kurano ◽  
Tamaki Kobayashi ◽  
Masako Nishikawa ◽  
Ryunosuke Ohkawa ◽  
...  
Keyword(s):  
Human Serum ◽  
Clinical Significance ◽  
Knockout Mice ◽  
Plasma Samples ◽  
Carrier Proteins ◽  
Serum Samples ◽  
Apolipoprotein M ◽  
Physiological Properties ◽  
Specific Manner ◽  
Sphingosine 1 Phosphate

Dihydro-sphingosine 1-phosphate (DH-S1P) is an analog of sphingosine 1-phosphate (S1P), which is a potent lysophospholipid mediator. DH-S1P has been proposed to exert physiological properties similar to S1P. Although S1P is known to be carried on HDL via apolipoprotein M (apoM), the association between DH-S1P and HDL/apoM has not been fully elucidated. Therefore, in the present study, we aimed to elucidate this association and to compare it with that of S1P and HDL/apoM. First, we investigated the distributions of S1P and DH-S1P among lipoproteins and lipoprotein-depleted fractions in human serum and plasma samples and observed that both S1P and DH-S1P were detected on HDL; furthermore, elevated amounts of DH-S1P in serum samples were distributed to the lipoprotein-depleted fraction to a greater degree than to the HDL fraction. Concordantly, a preference for HDL over albumin was only observed for S1P, and not for DH-S1P, when the molecules were secreted from platelets. Regarding the association with HDL, although both S1P and DH-S1P prefer to bind to HDL, HDL preferentially accepts S1P over DH-S1P. For the association with apoM, S1P was not detected on HDL obtained from apoM knockout mice, while DH-S1P was detected. Moreover, apoM retarded the degradation of S1P, but not of DH-S1P. These results suggest that S1P binds to HDL via apoM, while DH-S1P binds to HDL in a non-specific manner. Thus, DH-S1P is not a mere analog of S1P and might possess unique clinical significance.

LC–MS/MS measurement of 26 steroid hormones in human serum and plasma samples

2021 ◽  
Author(s):  
Yongjuan Jia ◽  
Xingli Liu ◽  
Li Xu ◽  
Jinbao Zhao ◽  
Junjun Ni ◽  
...  
Keyword(s):  
Human Serum ◽  
Steroid Hormones ◽  
Plasma Samples

Design and evaluation of standard lipid prediction models based on 1H-NMR spectroscopy of human serum/plasma samples

Metabolomics ◽  
2015 ◽  
Vol 11 (5) ◽  
pp. 1394-1404 ◽  
Author(s):  
Rubén Barrilero ◽  
Eduard Llobet ◽  
Roger Mallol ◽  
Jesús Brezmes ◽  
Lluis Masana ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Human Serum ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Prediction Models ◽  
Plasma Samples

scholarly journals Determination of the prevalence of the HBs antigen and evaluation of the vaccination status against Hepatitis B among the staff of the Dschang district hospital in West Cameroon

2021 ◽  
Vol 15 (2) ◽  
pp. 128-139
Author(s):  
Gouajeu Rodrigue ◽  
Djoko Ernest ◽  
Nzoume Nsope Marcel ◽  
Ngogang Jeanne
Keyword(s):  
Risk Factors ◽  
Hepatitis B ◽  
Health Personnel ◽  
District Hospital ◽  
Unprotected Sex ◽  
Hepatitis B Vaccine ◽  
Sub Saharan Africa ◽  
Hbs Antigen ◽  
Paramedical Staff ◽  
Hbs Ag

Hepatitis B infection is a major global health problem. Sub–saharan Africa is particularly affected. The aim of this study was to determine the prevalence of HBs antigen (HBs Ag) and to assess the hepatitis B vaccine status among medical and paramedical staff at Dschang District Hospital. A descriptive cross-sectional study was conducted at Dschang District Hospital from November 2018 to June 2019. All medical and paramedical staff of the Dschang District Hospital were included, regardless of sex or age, vaccinated and unvaccinated. We assessed the level of knowledge of hepatitis B, investigated hepatitis B markers like HBs antigen (HBs Ag), HBc antibody (HBc Ab), HBs antibody (HBs Ab); we performed HBs Ab assay, investigated hepatitis B risk factors, and low hepatitis B immunization factors, among an average of 171 health personnel aged 36.48 ± 9.58. Of these, 94.7 % said they knew hepatitis B. The prevalence of hepatitis B was 7%. Nursing and paramedical professions were the main concerned by the HBs Ag positivity. The risk factors found in HBs Ag carriers were unprotected sex, scarification and blood exposure accident. There was an association between HBs antigen portage and unprotected sex (P=0.021), blood exposure accident (P=0.021) and piercing (P=0.004). However, there was no association between HBs Ag carrying and age group (P=0.779), sex (P=0.248) and marital status (P=0.779). On the basis of the statements, without a certificate, 57% of the staff said they had already been vaccinated against hepatitis B; of these, 89% had received at least 3 doses of vaccine, of which 53.3% had acquired immunity from HBV. Advanced age (over 40 years) and overweight were associated with a low response to hepatitis B vaccine. In addition, sex and smoking were not associated with low hepatitis B seroprotection. In conclusion, this study found that the prevalence of HBs Ag is still high in our context and low hepatitis B seroprotection in the study population. Hence the need for awareness-raising, systematic screening and vaccination of health personnel, training of vaccinators and monitoring of the vaccination chain.

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