LC–MS/MS measurement of 26 steroid hormones in human serum and plasma samples

2021 ◽  
Author(s):  
Yongjuan Jia ◽  
Xingli Liu ◽  
Li Xu ◽  
Jinbao Zhao ◽  
Junjun Ni ◽  
...  
Keyword(s):  
Human Serum ◽  
Steroid Hormones ◽  
Plasma Samples

scholarly journals Dihydro-sphingosine 1-phosphate interacts with carrier proteins in a manner distinct from that of sphingosine 1-phosphate

2018 ◽  
Vol 38 (5) ◽  
Author(s):  
Yuko Mishima ◽  
Makoto Kurano ◽  
Tamaki Kobayashi ◽  
Masako Nishikawa ◽  
Ryunosuke Ohkawa ◽  
...  
Keyword(s):  
Human Serum ◽  
Clinical Significance ◽  
Knockout Mice ◽  
Plasma Samples ◽  
Carrier Proteins ◽  
Serum Samples ◽  
Apolipoprotein M ◽  
Physiological Properties ◽  
Specific Manner ◽  
Sphingosine 1 Phosphate

Dihydro-sphingosine 1-phosphate (DH-S1P) is an analog of sphingosine 1-phosphate (S1P), which is a potent lysophospholipid mediator. DH-S1P has been proposed to exert physiological properties similar to S1P. Although S1P is known to be carried on HDL via apolipoprotein M (apoM), the association between DH-S1P and HDL/apoM has not been fully elucidated. Therefore, in the present study, we aimed to elucidate this association and to compare it with that of S1P and HDL/apoM. First, we investigated the distributions of S1P and DH-S1P among lipoproteins and lipoprotein-depleted fractions in human serum and plasma samples and observed that both S1P and DH-S1P were detected on HDL; furthermore, elevated amounts of DH-S1P in serum samples were distributed to the lipoprotein-depleted fraction to a greater degree than to the HDL fraction. Concordantly, a preference for HDL over albumin was only observed for S1P, and not for DH-S1P, when the molecules were secreted from platelets. Regarding the association with HDL, although both S1P and DH-S1P prefer to bind to HDL, HDL preferentially accepts S1P over DH-S1P. For the association with apoM, S1P was not detected on HDL obtained from apoM knockout mice, while DH-S1P was detected. Moreover, apoM retarded the degradation of S1P, but not of DH-S1P. These results suggest that S1P binds to HDL via apoM, while DH-S1P binds to HDL in a non-specific manner. Thus, DH-S1P is not a mere analog of S1P and might possess unique clinical significance.

Design and evaluation of standard lipid prediction models based on 1H-NMR spectroscopy of human serum/plasma samples

Metabolomics ◽  
2015 ◽  
Vol 11 (5) ◽  
pp. 1394-1404 ◽  
Author(s):  
Rubén Barrilero ◽  
Eduard Llobet ◽  
Roger Mallol ◽  
Jesús Brezmes ◽  
Lluis Masana ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Human Serum ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Prediction Models ◽  
Plasma Samples

Identification of the major steroids in ovarian and adrenal venous plasma of the brush-tail possum (Trichosurus vulpecula) and changes in the peripheral plasma levels of oestradiol and progesterone during the reproductive cycle

1985 ◽  
Vol 105 (1) ◽  
pp. 53-62 ◽  
Author(s):  
J. D. Curlewis ◽  
M. Axelson ◽  
G. M. Stone
Keyword(s):  
Steroid Hormones ◽  
Reproductive Cycle ◽  
Limit Of Detection ◽  
Peak Level ◽  
Gas Chromatography Mass Spectrometry ◽  
Plasma Samples ◽  
Ovarian Vein ◽  
Blood Samples ◽  
Peripheral Plasma ◽  
Venous Plasma

ABSTRACT The quantitatively major steroid hormones in ovarian and adrenal venous plasma of the female brush-tail possum were identified by gas chromatography–mass spectrometry. The ovarian vein plasma samples all contained oestradiol and its concentration was highest during the pro-oestrous phase of the reproductive cycle. During this phase the concentration of progesterone was below the limit of detection but at day 13 of the oestrous cycle and pregnancy, the concentration of progesterone exceeded that of oestradiol. Cortisol and corticosterone were the major steroid hormones found in all adrenal vein samples with cortisol predominant. Androgens with a 3-oxo structure, if present, were below the limits of detection in all plasma samples. Radioimmunoassays for the measurement of progesterone and oestradiol in peripheral plasma were used to follow changes in the concentrations of these steroids during the reproductive cycle. Progesterone in serial blood samples was low at oestrus, rose gradually until day 7 and then increased more rapidly to reach a peak level of 21–29 nmol/l at around day 13. Any differences between the pregnant and non-pregnant cycles were minor. Oestradiol was only detected around oestrus when levels were variable (53·3±20·92 (s.e.m.) pmol/l; n = 4). The results indicate that the reproductive cycle of the brush-tail possum is characterized by a single peak of oestradiol at around pro-oestrus followed by gradually increasing levels of progesterone. Pregnancy appears to have no influence on the circulating concentrations of oestradiol or progesterone. J. Endocr. (1985) 105, 53–62

scholarly journals Human Serum Albumin Cys34Oxidative Modifications following Infiltration in the Carotid Atherosclerotic Plaque

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Antonio Junior Lepedda ◽  
Angelo Zinellu ◽  
Gabriele Nieddu ◽  
Pierina De Muro ◽  
Ciriaco Carru ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Carotid Endarterectomy ◽  
Atherosclerotic Plaque ◽  
Plasma Samples ◽  
Carotid Atherosclerotic Plaque ◽  
Sds Page ◽  
Oxidative Modifications ◽  
First Time

Objectives.To evaluate if the prooxidant environment present in atherosclerotic plaque may oxidatively modify filtered albumin.Methods.Fluorescein-5-maleimide labelled plasma samples and plaque extracts from 27 patients who had undergone carotid endarterectomy were analysed through nonreducing SDS-PAGE for albumin-Cys34oxidation. Furthermore, degree and pattern of S-thiolation in both circulating and plaque-filtered albumin were assayed.Results.Albumin filtered in the atherosclerotic plaque showed higher levels of Cys34oxidative modifications than the corresponding circulating form as well as different patterns of S-thiolation.Conclusions.Data indicate that the circulating albumin, once filtered in plaque, undergoes Cys34oxidative modifications and demonstrate for the first time that albumin is a homocysteine and cysteinylglycine vehicle inside the plaque environment.

A Radioimmunoassay for Total Captopril in Human Serum or Plasma Samples

1984 ◽  
Vol 6 (1) ◽  
pp. 59-66 ◽  
Author(s):  
J. Tu ◽  
E. Liu ◽  
E. L. Nickoloff
Keyword(s):  
Human Serum ◽  
Plasma Samples
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