Sex-linked photosynthetic physiologic research and the evolutionary ecological analysis in living fossil plant, Ginkgo biloba L.

2008 ◽  
Vol 28 (3) ◽  
pp. 1128-1136 ◽  
Author(s):  
Jin Jing ◽  
Jiang Hong ◽  
Yu Shuquan ◽  
Zhou Guomo
Biologia ◽  
2008 ◽  
Vol 63 (4) ◽  
Author(s):  
Feng Xu ◽  
Rong Cai ◽  
Shui-yuan Cheng ◽  
Liu-ji Chen ◽  
Shu-han Cheng

AbstractThe chloroplast ATP synthase (ATPase) utilizes the energy of a transmembrane electrochemical proton gradient to drive the synthesis of ATP from ADP and phosphate. The chloroplast ATPase α and β subunits are the essential components of multisubunit protein complex. In this paper, the full-length cDNA and genomic DNA of ATPase α (designated as GbatpA) and β (designated as GbatpB) subunit genes were isolated from Ginkgo biloba. The GbatpA and GbatpB genes were both intronless. The coding regions of GbatpA and GbatpB were 1530 bp and 1497 bp long, respectively, and their deduced amino acid sequences showed high degrees of identity to those of other plant ATPase α and β proteins, respectively. The expression analysis by RT-PCR revealed that GbatpA and GbatpB both expressed in tissue-specific manners in G. biloba and might involve in leaf development. The recombinant GbATPB protein was successfully expressed in E. coli strain using pET28a vector with ATPase activity as three times high as the control, and the results showed that the molecular weight of the recombinant protein was about 54 kDa, a size that was in agreement with that predicted by bioinformatics analysis. This study provides useful information for further studying on overall structure, function and regulation of the chloroplast ATPase in G. biloba, the so-called “living fossil” plant as one of the oldest gymnosperm species.


2020 ◽  
Vol 2 (1) ◽  
pp. 29

Based on the global environmental pollution problems, the main focus of every nano-research is to produce the nanomaterial in a green and eco-friendly way without any interference of chemical synthesis. By the way, the present study was intended to use an aqueous extract of the living fossil plant viz., Ginkgo biloba L., to synthesize the gold nanoparticles and evaluate their antibiotic activity against bacterial pathogens. The gold nanoparticles (AuNps) were successfully synthesized by mixing the Ginkgo biloba aqueous extract and the auric chloride solution for approximately 24 hours. The UV-Vis spectra of Gold nanoparticles (AuNps) showed the maximum absorption peak at 520nm. The SEM analysis also showed the gold nanoparticles synthesized from Ginkgo biloba were spherical with particle size ranging from 40 to 60nm. During our study, the gold nanoparticles exhibited significant antimicrobial activity against bacterial pathogens, i.e., E. coli and Bacillus subtilis. The later bacterium was found to be more susceptible to the nanoparticles as well as the extracts of G. biloba in comparison to the former bacterium.


Forests ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 705 ◽  
Author(s):  
Ying Guo ◽  
Jing Guo ◽  
Xin Shen ◽  
Guibin Wang ◽  
Tongli Wang

Ginkgo (Ginkgo biloba L.) is not only considered a ‘living fossil’, but also has important ecological, economic, and medicinal values. However, the impact of climate change on the performance and distribution of this plant is an increasing concern. In this study, we developed a bioclimatic model based on data about the occurrence of ginkgo from 277 locations, and validated model predictions using a wide-ranging field test (12 test sites, located at the areas from 22.49° N to 39.32° N, and 81.11° E to 123.53° E). We found that the degree-days below zero were the most important climate variable determining ginkgo distribution. Based on the model predictions, we classified the habitat suitability for ginkgo into four categories (high, medium, low, and unsuitable), accounting for 9.29%, 6.09%, 8.46%, and 76.16% of China’s land area, respectively. The ANOVA results of the validation test showed significant differences in observed leaf-traits among the four habitat types (p < 0.05), and importantly the rankings of the leaf traits were consistent with our classification of the habitat suitability, suggesting the effectiveness of our classification in terms of biological and economic significance. In addition, we projected that suitable (high and medium) habitats for ginkgo would shrink and shift northward under both the RCP4.5 and RCP8.5 climate change scenarios for three future periods (the 2020s, 2050s, and 2080s). However, the area of low-suitable habitat would increase, resulting in a slight decrease in unsuitable habitats. Our findings contribute to a better understanding of climate change impact on this plant and provide a scientific basis for developing adaptive strategies for future climate.


2019 ◽  
Author(s):  
He Zhang ◽  
Rui Zhang ◽  
Xianwei Yang ◽  
Kai-Jie Gu ◽  
Wenbin Chen ◽  
...  

ABSTRACTSexual dimorphism like dioecy (separate male and female individuals) have evolved in diverse multicellular eukaryotes while the molecular mechanisms underlying the development of such a key biological trait remains elusive (1). The living fossil Ginkgo biloba represents an early diverged lineage of land plants with dioecy. However, its sex-determination system and molecular basis have long been controversial or unknown. In the present research, we assembled the first and largest to date chromosome-level genome of a non-model tree species using Hi-C data. With this reference genome, we addressed both questions using genome resequencing data gathered from 97 male and 265 female trees of ginkgo, as well as transcriptome data from three developmental stages for both sexes. Our results support vertebrate-like XY chromosomes for ginkgo and five potential sex-determination genes, which may originate ~14 million years ago. This is the earliest diverged sex determination region in all reported plants as yet. The present research resolved a long-term controversy, lay a foundation for future studies on the origin and evolution of plant sexes, and provide genetic markers for sex identification of ginkgo which will be valuable for both nurseries and field ecology of ginkgo.


2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Petr Šmarda ◽  
Lucie Horová ◽  
Ondřej Knápek ◽  
Heidi Dieck ◽  
Martin Dieck ◽  
...  
Keyword(s):  

2016 ◽  
Vol 66 (5) ◽  
pp. 2013-2018 ◽  
Author(s):  
Huan Xu ◽  
Sheng Zhang ◽  
Juan Cheng ◽  
Mipeshwaree Devi Asem ◽  
Meng-Yue Zhang ◽  
...  

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