scholarly journals Novel dual role of dendritic cells in priming de novo CTL responses while inhibiting memory CTL responses to HIV-1 through the PD-L1 pathway

2017 ◽  
Vol 3 ◽  
pp. 36
Author(s):  
T.M. Garcia-Bates ◽  
M. Palma ◽  
B. Macatangay ◽  
C. Rinaldo ◽  
R. Mailliard
PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0156063 ◽  
Author(s):  
Besma Aouar ◽  
Denisa Kovarova ◽  
Sebastien Letard ◽  
Albert Font-Haro ◽  
Jonathan Florentin ◽  
...  

2004 ◽  
Vol 200 (10) ◽  
pp. 1279-1288 ◽  
Author(s):  
Jean-François Arrighi ◽  
Marjorie Pion ◽  
Eduardo Garcia ◽  
Jean-Michel Escola ◽  
Yvette van Kooyk ◽  
...  

Dendritic cells (DCs) are essential for the early events of human immunodeficiency virus (HIV) infection. Model systems of HIV sexual transmission have shown that DCs expressing the DC-specific C-type lectin DC-SIGN capture and internalize HIV at mucosal surfaces and efficiently transfer HIV to CD4+ T cells in lymph nodes, where viral replication occurs. Upon DC–T cell clustering, internalized HIV accumulates on the DC side at the contact zone (infectious synapse), between DCs and T cells, whereas HIV receptors and coreceptors are enriched on the T cell side. Viral concentration at the infectious synapse may explain, at least in part, why DC transmission of HIV to T cells is so efficient. Here, we have investigated the role of DC-SIGN on primary DCs in X4 HIV-1 capture and transmission using small interfering RNA–expressing lentiviral vectors to specifically knockdown DC-SIGN. We demonstrate that DC-SIGN− DCs internalize X4 HIV-1 as well as DC-SIGN+ DCs, although binding of virions is reduced. Strikingly, DC-SIGN knockdown in DCs selectively impairs infectious synapse formation between DCs and resting CD4+ T cells, but does not prevent the formation of DC–T cells conjugates. Our results demonstrate that DC-SIGN is required downstream from viral capture for the formation of the infectious synapse between DCs and T cells. These findings provide a novel explanation for the role of DC-SIGN in the transfer and enhancement of HIV infection from DCs to T cells, a crucial step for HIV transmission and pathogenesis.


2010 ◽  
Vol 6 (4) ◽  
pp. e1000862 ◽  
Author(s):  
Marie-Thérèse Melki ◽  
Héla Saïdi ◽  
Alexandre Dufour ◽  
Jean-Christophe Olivo-Marin ◽  
Marie-Lise Gougeon

Blood ◽  
2009 ◽  
Vol 114 (1) ◽  
pp. 85-94 ◽  
Author(s):  
Andrew N. Harman ◽  
Marianne Kraus ◽  
Chris R. Bye ◽  
Karen Byth ◽  
Stuart G. Turville ◽  
...  

AbstractDendritic cells (DCs) play a key role in the pathogenesis of HIV infection. HIV interacts with these cells through 2 pathways in 2 temporal phases, initially via endocytosis and then via de novo replication. Here the transcriptional response of human DCs to HIV-1 was studied in these phases and at different stages of the virus replication cycle using purified HIV-1 envelope proteins, and inactivated and viable HIV-1. No differential gene expression was detected in response to envelope. However, more than 100 genes were differentially expressed in response to entry of viable and inactivated HIV-1 in the first phase. A completely different set of genes was differentially expressed in the second phase, predominantly in response to viable HIV-1, including up-regulation of immune regulation genes, whereas genes encoding lysosomal enzymes were down-regulated. Cathepsins B, C, S, and Z RNA and protein decreased, whereas cathepsin L was increased, probably reflecting a concomitant decrease in cystatin C. The net effect was markedly diminished cathepsin activity likely to result in enhanced HIV-1 survival and transfer to contacting T lymphocytes but decreased HIV-1 antigen processing and presentation to these T cells.


Development ◽  
2020 ◽  
Vol 147 (24) ◽  
pp. dev191494
Author(s):  
Andrew C. Symonds ◽  
Clare E. Buckley ◽  
Charlotte A. Williams ◽  
Jonathan D. W. Clarke

ABSTRACTUsing the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that Mpp5a and Rab11a play a dual role in coordinating the generation of ipsilateral junctional belts whilst simultaneously releasing contralateral adhesions across the centre of the tissue. We show that Mpp5a- and Rab11a-mediated resolution of cell-cell adhesions are both necessary for midline lumen opening and contribute to later maintenance of epithelial organisation. We propose that these roles for both Mpp5a and Rab11a operate through the transmembrane protein Crumbs. In light of a recent conflicting publication, we also clarify that the junction-remodelling role of Mpp5a is not specific to dividing cells.


2005 ◽  
Vol 115 (3) ◽  
pp. 765-773 ◽  
Author(s):  
Theresa L. Chang ◽  
Jesus Vargas ◽  
Armando DelPortillo ◽  
Mary E. Klotman
Keyword(s):  
Anti Hiv ◽  

2014 ◽  
Vol 11 (1) ◽  
pp. 23 ◽  
Author(s):  
Guangyu Li ◽  
Mark A Endsley ◽  
Anoma Somasunderam ◽  
Sonia L Gbota ◽  
Maryann I Mbaka ◽  
...  
Keyword(s):  
Hiv 1 ◽  

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