Effects of grape seed extract supplementation on exercise-induced oxidative stress in rats

The aim of the present study was to investigate the effects of grape seed extract (GSE) supplementation on exercise performance and oxidative stress in acutely and chronically exercised rats. A total of sixty-four male rats were used in the study. Rats were divided into six groups: control, chronic exercise control, acute exercise control (AEC), GSE-supplemented control, GSE-supplemented chronic exercise and GSE-supplemented acute exercise groups. Chronic exercise consisted of treadmill running at 25 m/min, 45 min/d, 5 d a week for 6 weeks. Rats in the acute exercise groups were run on the treadmill at 30 m/min until exhaustion. GSE were given at 100 mg/kg of body weight with drinking water for 6 weeks. Plasma was separated from blood samples for the analysis of oxidative stress markers. There was no significant difference in time of exhaustion between the acute exercise groups. Plasma malondialdehyde (MDA) levels were higher in the acute exercise groups and lower in the chronic exercise groups. GSE supplementation decreased MDA levels. Xanthine oxidase and adenosine deaminase activities were higher in the AEC group compared to all the other groups. NO levels were increased with both chronic exercise and GSE supplementation. Superoxide dismutase and glutathione peroxidase activities were lower in the acute exercised groups and higher in the chronic exercised groups. GSE supplementation caused an increase in antioxidant enzyme activities. In conclusion, GSE supplementation prevents exercise-induced oxidative stress by preventing lipid peroxidation and increasing antioxidant enzyme activities.

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The effects of grape seed extract supplementation on exercise-induced oxidative stress in young untrained males

Iranian Journal Of Health Sciences ◽

10.18502/jhs.v9i4.8218 ◽

2022 ◽

Author(s):

Hamid Reza Zolfi ◽

Vahid Sari-Sarraf ◽

Hossein Babaei ◽

Amirmansour Vatankhah

Keyword(s):

Oxidative Stress ◽

Aerobic Exercise ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Controlled Study ◽

Total Antioxidant ◽

Exercise Induced

Background and Purpose: The aim of this study was to determine the effect of acute aerobic exercise and 14-days grape seed extract supplementation on total antioxidant capacity, lipid peroxidation and muscle cell damage biomarkers in untrained males Materials and Methods: In a randomized, double-blinded, and placebo-controlled study, twenty-two male students (age 19±1 years, weight 67.44±7 kg, BMI 22±2, V̇ O2max 39±2 ml/kg-1 /min-1 ) were randomly assigned to two groups of grape seed extract and placebo (PLA) (GSE: 200 mg/day for two weeks). After subsequent 14-days of supplementation, subjects did a single session of aerobic exercise (running) on treadmill at 75% VO2max for 30 minutes. Blood samples were taken 3 times: prior to supplementation (baseline), 14-days after supplementation, and immediately after exercise (post-exercise). Total antioxidant capacity (TAC), malondialdehyde (MDA), creatine kinase (CK), lactate dehydrogenase (LDH) and lactate were measured. TAC was determined by ABTs method. The collected data were then analyzed by running analysis of variance (ANOVA) with repeated measure and Bonferroni post-hoc tests as appropriate using SPSS17 at p<0.05. Results: Malondialdehyde, CK, LDH, Lactate were significantly increased after aerobic exercise (p<0.05). Short-term GSE supplementation significantly prevented MDA and CK cascade after exercise compared to PLA (p<0.05) but, it had no significant effect on basal parameters (p>0.05). The exercise had also no significant effect on total antioxidant capacity in any of the groups (p>0.05). Conclusion: Aerobic exercise could increase blood oxidative stress biomarkers and GSE supplementation, due to influential antioxidant effect; yet, it could attenuate exercise-induced oxidative stress in men

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Grape seed extract protects against amiodarone - induced nephrotoxicity and ultrastructural alterations associated with the inhibition of biomarkers of inflammation and oxidative stress in rats

Ultrastructural Pathology ◽

10.1080/01913123.2020.1864076 ◽

2021 ◽

pp. 1-10

Author(s):

Refaat A. Eid ◽

Mohamed Samir Ahmed Zaki ◽

Mubarak Al-Shraim ◽

Muhammad Alaa Eldeen ◽

Mohamed A. Haidara

Keyword(s):

Oxidative Stress ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Ultrastructural Alterations ◽

And Oxidative Stress

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Effects of prolonged stanozolol treatment on antioxidant enzyme activities, oxidative stress markers, and heat shock protein HSP72 levels in rat liver

The Journal of Steroid Biochemistry and Molecular Biology ◽

10.1016/j.jsbmb.2003.09.001 ◽

2003 ◽

Vol 87 (4-5) ◽

pp. 269-277 ◽

Cited By ~ 40

Author(s):

Angel Pey ◽

Ana Saborido ◽

Isabel Blázquez ◽

Jerónimo Delgado ◽

Alicia Megı́as

Keyword(s):

Oxidative Stress ◽

Heat Shock ◽

Heat Shock Protein ◽

Antioxidant Enzyme ◽

Rat Liver ◽

Enzyme Activities ◽

Antioxidant Enzyme Activities ◽

Oxidative Stress Markers ◽

Stress Markers

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Effects of Cadmium on Growth, Oxidative Stress and Antioxidant Enzyme Activities in Peanut (Arachis hypogaea L.) Seedlings

Journal of Agricultural Science ◽

10.5539/jas.v4n6p142 ◽

2012 ◽

Vol 4 (6) ◽

Cited By ~ 1

Author(s):

Shihua Shan ◽

Feng Liu ◽

Chunjuan Chunjuan Li ◽

Shubo Wan

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzyme ◽

Arachis Hypogaea ◽

Enzyme Activities ◽

Antioxidant Enzyme Activities ◽

Arachis Hypogaea L

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Antioxidants and selenocompounds inhibit 3,5-dimethylaminophenol toxicity to human urothelial cells

Archives of Industrial Hygiene and Toxicology ◽

10.2478/aiht-2019-70-3159 ◽

2019 ◽

Vol 70 (1) ◽

pp. 18-29 ◽

Cited By ~ 4

Author(s):

Pinar Erkekoglu ◽

Ming-Wei Chao ◽

Chia-Yi Tseng ◽

Bevin P. Engelward ◽

Ozge Kose ◽

...

Keyword(s):

Oxidative Stress ◽

Bladder Cancer ◽

Antioxidant Enzyme ◽

Enzyme Activities ◽

Mammalian Cells ◽

Antioxidant Enzyme Activities ◽

Urothelial Cells ◽

Dna Breaks ◽

Primary Metabolite ◽

Stress Changes

AbstractExposure to alkyl anilines may lead to bladder cancer, which is the second most frequent cancer of the urogenital tract. 3,5-dimethylaniline is highly used in industry. Studies on its primary metabolite 3,5-dimethylaminophenol (3,5-DMAP) showed that this compound causes oxidative stress, changes antioxidant enzyme activities, and leads to death of different mammalian cells. However, there is no in vitro study to show the direct effects of 3,5-DMAP on human bladder and urothelial cells. Selenocompounds are suggested to decrease oxidative stress caused by some chemicals, and selenium supplementation was shown to reduce the risk of bladder cancer. The main aim of this study was to investigate whether selenocompounds organic selenomethionine (SM, 10 µmol/L) or inorganic sodium selenite (SS, 30 nmol/L) could reduce oxidative stress, DNA damage, and apoptosis in UROtsa cells exposed to 3,5-DMAP. 3,5-DMAP caused a dose-dependent increase in intracellular generation of reactive oxygen species, and its dose of 50 µmol/L caused lipid peroxidation, protein oxidation, and changes in antioxidant enzyme activities in different cellular fractions. The comet assay also showed single-strand DNA breaks induced by the 3,5-DMAP dose of 50 µmol/L, but no changes in double-strand DNA breaks. Apoptosis was also triggered. Both selenocompounds provided partial protection against the cellular toxicity of 3,5-DMAP. Low selenium status along with exposure to alkyl anilines can be a major factor in the development of bladder cancer. More mechanistic studies are needed to specify the role of selenium in bladder cancer.

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