Cuticular and internal n-alkane composition of Lucilia sericata larvae, pupae, male and female imagines: application of HPLC-LLSD and GC/MS-SIM

2012 ◽  
Vol 102 (4) ◽  
pp. 453-460 ◽  
Author(s):  
M. Gołębiowski ◽  
M. Paszkiewicz ◽  
A. Grubba ◽  
D. Gąsiewska ◽  
M.I. Boguś ◽  
...  
Keyword(s):  
Lucilia Sericata ◽  
Cuticular Lipids ◽  
Ion Current ◽  
Hydrocarbon Fraction ◽  
Adult Males ◽  
Selected Ion Monitoring ◽  
Male And Female ◽  
Carbon Chains ◽  
Males And Females

AbstractThe composition of cuticular and internal n-alkanes in Lucilia sericata larvae, pupae, and male and female imagines were studied. The cuticular and internal lipid extracts were separated by HPLC-LLSD, after which the hydrocarbon fraction was identified by GC/MS in selected ion monitoring (SIM) and total ion current (TIC) modes.The cuticular lipids of the larvae contained seven n-alkanes from C23 to C31. The major n-alkane in L. sericata larvae was C29 (42.1%). The total cuticular n-alkane content in the cuticular lipids was 31.46 μg g−1 of the insect body. The internal lipids of L. sericata larvae contained five n-alkanes ranged from C25 to C31. The most abundant compound was C27 (61.71 μg g−1 of the insect body). Eighteen n-alkanes from C14 to C31 were identified in the cuticular lipids of the pupae. The most abundant n-alkanes ranged from C25 to C31; those with odd-numbered carbon chains were particularly abundant, the major one being C29:0 (59.5%). Traces of eight cuticular n-alkanes were present. The internal lipids of L. sericata pupae contained five n-alkanes, ranging from C25 to C31. The cuticular lipids of female imagines contained 17 n-alkanes from C12 to C30. Among the cuticular n-alkanes of females, C27 (47.5%) was the most abundant compound. Four n-alkanes, with only odd-numbered carbon chains, were identified in the internal lipids of females. The lipids from both sexes of L. sericata had similar n-alkane profiles. The cuticular lipids of adult males contained 16 n-alkanes ranging from C13 to C31. C27 (47.9%) was the most abundant cuticular n-alkanes in males. The same n-alkanes only with odd-numbered carbon chains and in smaller quantities of C27 (0.1%) were also identified in the internal lipids of males.The highest amounts of total cuticular n-alkanes were detected in males and females of L. sericata (330.4 and 158.93 μg g−1 of the insect body, respectively). The quantities of total cuticular alcohols in larvae and pupae were smaller (31.46 μg g−1 and 42.08 μg g−1, respectively). The internal n-alkane contents of larvae, pupae, and male and female imagines were significantly higher than the cuticular n-alkane contents (153.53, 99.60, 360.06 and 838.76 μg g−1 of the insect body, respectively).

GONADOTROPHIN PATTERNS IN MALE AND FEMALE RATS: PLASMA LH AND FSH AT VARIOUS AGES EVALUATED BY THE METHOD OF PARABIOSIS

1967 ◽  
Vol 56 (1) ◽  
pp. 165-176 ◽  
Author(s):  
Donald C. Johnson
Keyword(s):  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Female Rats ◽  
Adult Males ◽  
Male And Female ◽  
Ovarian Weight ◽  
Average Value ◽  
Male And Female Rats ◽  
Tonic Level ◽  
Males And Females

ABSTRACT Ventral prostates in hypophysectomized male parabiotic partners of intact animals were used to compare the amount of plasma luteinizing hormone (LH) in males and females of various ages. Ovarian weight, histology, and augmentation with chorionic gonadotrophin, in hypophysectomized androgenized females were used to estimate plasma follicle stimulating hormone (FSH) activity in intact adult males and females. In young animals, up to 50 days of age, males apparently have the same amount of plasma LH as females, but older cyclic females produced significantly heavier prostates in their hypophysectomized male partners than did males. The results are consistent with the interpretation that cyclic surges of LH added to a tonic level produced an average value higher for females than males. In contrast, males of all ages and particularly adults, have a significantly greater amount of circulating FSH than females.

The antifungal activity of the cuticular and internal fatty acid methyl esters and alcohols in Calliphora vomitoria

Parasitology ◽  
2013 ◽  
Vol 140 (8) ◽  
pp. 972-985 ◽  
Author(s):  
MAREK GOŁĘBIOWSKI ◽  
MAGDALENA CERKOWNIAK ◽  
MAŁGORZATA DAWGUL ◽  
WOJCIECH KAMYSZ ◽  
MIECZYSŁAWA I. BOGUŚ ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Acid Methyl Ester ◽  
Cuticular Lipids ◽  
Carbon Chains ◽  
Acid Methyl ◽  
Males And Females ◽  
The Individual

SUMMARYThe composition of the fatty acid methyl ester (FAME) and alcohol fractions of the cuticular and internal lipids of Calliphora vomitoria larvae, pupae and male/female adults was obtained by separating these two fractions by HPLC–LLSD and analysing them quantitatively using GC–MS. Analysis of the cuticular lipids of the worldwide, medically important ectoparasite C. vomitoria revealed 6 FAMEs with odd-numbered carbon chains from C15:0 to C19:0 in the larvae, while internal lipids contained 9 FAMEs ranging from C15:1 to C19:0. Seven FAMEs from C15:0 to C19:0 were identified in the cuticular lipids of the pupae, whereas the internal lipids of the pupae contained 10 FAMEs from C13:0 to C19:0. The cuticular lipids of males and females and also the internal lipids of males contained 5, 7 and 6 FAMEs from C15:0 to C19:0 respectively. Seven FAMEs from C13:0 to C19:0 were identified in the internal lipids of females, and 7, 6, 5 and 3 alcohols were found in the cuticular lipids of larvae, pupae, males and females respectively. Only saturated alcohols with even-numbered carbon chains were present in these lipids. Only 1 alcohol (C22:0) was detected in the internal lipids of C. vomitoria larvae, while just 4 alcohols from – C18:0 to C24:0 – were identified in the internal lipids of pupae, and males and females. We also identified glycerol and cholesterol in the larvae, pupae, males and females of C. vomitoria. The individual alcohols and FAMEs, as well as their mixtures isolated from the cuticular and internal lipids of larvae, pupae, males and females of C. vomitoria, demonstrated antimicrobial activity against entomopathogenic fungi.

scholarly journals Multiple Toll-Spätzle Pathways in Drosophila melanogaster Immunity

2018 ◽  
Author(s):  
Munmun Chowdhury ◽  
Chun-Feng Li ◽  
Zhen He ◽  
Yuzhen Lu ◽  
Xusheng Liu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drosophila Melanogaster ◽  
Candida Albicans ◽  
Systemic Infection ◽  
Defense Responses ◽  
Immune Defense ◽  
Adult Males ◽  
Male And Female ◽  
Vesicular Stomatitis ◽  
Males And Females

AbstractThe Drosophila melanogaster Toll-Spätzle pathway plays an important role in development and immunity. Drosophila genome encodes nine Toll receptors and six Spätzle (Spz) proteins, and only the canonical Toll-Spz (Toll-1-Spz-1) pathway has been well investigated. In this study, we compared the nine Drosophila Tolls and showed that similarly to Toll, Toll-7 also strongly activated drosomycin promoter. Importantly, we showed that both Toll and Toll-7 interacted with Spz, Spz-2 and Spz-5, and co-expression of Toll or Toll-7 with Spz, Spz-2 and Spz-5 activated the drosomycin promoter. Furthermore, Toll and Toll-7 both recognized vesicular stomatitis virus (VSV) by binding to the VSV glycoprotein. Septic infection in Toll and Toll-7 mutant flies suggested that Toll and Toll-7 differentially affected defense responses in adult males and females after systemic infection by Enterococcus faecalis, Pseudomonas aeruginosa, Candida albicans or VSV. Our results suggest multiple Toll family members activate the expression of antimicrobial peptides. Our results also provide evidence that Toll and Toll-7 bind multiple Spätzle proteins and differentially affect immune defense against different pathogens in adult male and female flies.

Roads, routes and rams: does sexual segregation contribute to anthropogenic risk in a desert-dwelling ungulate?

2016 ◽  
Vol 43 (5) ◽  
pp. 380 ◽  
Author(s):  
Vernon C. Bleich ◽  
Jericho C. Whiting ◽  
John G. Kie ◽  
R. Terry Bowyer
Keyword(s):  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Sexual Segregation ◽  
Adult Males ◽  
Large Mammals ◽  
Male And Female ◽  
Use Of Resources ◽  
Core Areas ◽  
Males And Females ◽  
Time Of Year

Context Little is known about the consequences of sexual segregation (differential use of resources by the sexes outside of the mating season) for the conservation of large mammals. Roadways (i.e. the strip of land over which a road or route passes) are ubiquitous around the world, and are a major cause of wildlife mortality, as well as habitat loss and fragmentation. Many populations of bighorn sheep (Ovis canadensis) occur at low densities and in a metapopulation structure. Roadways could affect movements of males and females differentially, an outcome that has not been considered previously. Aims We investigated the propensity of the sexes to cross a paved two-lane road and a single-lane, maintained dirt route and predicted that adult males, because of their life-history characteristics, would cross those roadways more often than females. Methods We investigated movements of male and female bighorn sheep from 1986 to 1990. We used a fixed-wing aircraft with an H-antenna on each wing strut to locate individuals each week from October 1986 to December 1990. We estimated the degree of overlap among 50% core areas of use by males and females with the utilisation distribution overlap index (UDOI). Key results We relocated male and female bighorn sheep on 948 occasions during sexual aggregation and on 1951 occasions during sexual segregation. More males than females were likely to cross both types of roadways during segregation, and the dirt route during aggregation. Propensity of males and females to cross roadways was strongly influenced by time of year (i.e. whether the period of sexual aggregation or sexual segregation). The lowest overlap in 50% core areas was between females and males during periods of segregation (UDOI = 0.1447). Conclusions More males than females crossed Kelbaker Road and the unnamed dirt route during segregation, as well as the unnamed route during aggregation. Both of those features could affect males more than females, and could result in reductions in the use of habitat or increased mortality of bighorn sheep from vehicle collisions as a result of spatial segregation of the sexes. Implications During environmental review, biologists should consider sexual segregation when assessing potential anthropogenic effects on movements of bighorn sheep. Biologists also should consider sexual segregation and how roadways, even lightly traveled routes, affect movements of male and female ungulates differently before manipulating habitat, translocating animals, or constructing or modifying roadways.

Impact of age and sex on response to ischemic preconditioning in the rat heart: differential role of the PI3K–AKT pathway

2013 ◽  
Vol 91 (8) ◽  
pp. 640-647 ◽  
Author(s):  
Veronika Ledvenyiova ◽  
Dezider Pancza ◽  
Jana Matejiková ◽  
Miroslav Ferko ◽  
Iveta Bernatova ◽  
...  
Keyword(s):  
Ischemic Preconditioning ◽  
Ischemia Reperfusion ◽  
Akt Pathway ◽  
Female Rat ◽  
Adult Males ◽  
Male And Female ◽  
Males And Females ◽  
The Impact ◽  
Old Females

Sex and aging represent important factors that determine morbidity and mortality due to cardiovascular diseases in the human population. This study aimed to investigate the impact of aging on the response to ischemia–reperfusion in male and female rat hearts, and to explore a potential role of the PI3K–Akt pathway in the cardioprotective effects of ischemic preconditioning (IPC) in the myocardium of younger and older adult males and females. Langendorff-perfused nonpreconditioned and preconditioned hearts of 12- and 18-week-old male and female Wistar rats were subjected to regional ischemia and reperfusion with or without prior perfusion with the PI3K inhibitor wortmannin for the evaluation of ischemia-induced arrhythmias and the size of myocardial infarction (infarct size; IS). Aging did not modify IS in both sexes; however, it markedly increased susceptibility to arrhythmias. Although IPC effectively reduced IS in males and females of both ages, only the hearts of males and 18-week-old females benefited from its antiarrhythmic effect. In the preconditioned 12-week-old females, but not the 18-week-old females, and in males of both ages, wortmannin blunted the anti-infarct effect of IPC. In conclusion, activation of the PI3K–Akt pathway plays an important role in protection against lethal injury conferred by IPC in males irrespective of age. The IS-limiting effect of IPC appears to be PI3K–Akt-dependent only in the 12-week-old females.

scholarly journals Description of life stages of dung beetle Scaptodera rhadamistus (Fabricius, 1775) (Coleoptera: Scarabaeidae: Scarabaeinae) with notes on nesting and biology

2018 ◽  
Vol 10 (15) ◽  
pp. 12990-12994
Author(s):  
Suvarna S. Khadakkar ◽  
Ashish D. Tiple ◽  
Arun M. Khurad
Keyword(s):  
Dung Beetle ◽  
Female Genitalia ◽  
Immature Stages ◽  
Life Stages ◽  
Adult Males ◽  
Male And Female ◽  
Males And Females ◽  
First Time ◽  
Support Projection

Immature stages of Scaptodera rhadamistus (Fabricius) are described for the first time along with notes on nidification and biology.  The larvae differ from other Scarabaeinae species in the structure of raster on tenth sternum with two irregular bunches of serrations ventrally one on either half.  Pupae with pronotum transverse having rounded margins resemble adults, and consist of four lateral, single caudal and single pteronotal support projection.  Adult males and females differ in coloration, structure of pronotum, presence of spine like process on mesosternum and, in the structure of male and female genitalia.

Gyrinus rhyssonotum sp. n., a new species of Gyrinidae (Coleoptera: Adephaga) from northern Brazil

Zootaxa ◽  
2019 ◽  
Vol 4560 (1) ◽  
pp. 164
Author(s):  
DANIARA COLPANI ◽  
CESAR JOÃO BENETTI ◽  
NEUSA HAMADA
Keyword(s):  
New Species ◽  
Body Shape ◽  
The Body ◽  
Female Genitalia ◽  
Adult Males ◽  
Male And Female ◽  
Northern Brazil ◽  
Males And Females ◽  
Amazonas State ◽  
A New Species

Gyrinus rhyssonotum sp. n. is described and illustrated based on adult males and females collected in Barcelos County, Amazonas state, Brazil. The new species belongs to the subgenus Oreogyrinus Ochs, 1935 and it is compared with Gyrinus (Oreogyrinus) fittkaui Ochs, 1963 and G. (O.) opalinus Régimbart, 1883, both occurring in the Brazilian Amazonas and G. (O.) colombicus Régimbart, 1883 and G. (O.) venezolensis Ochs, 1954, these two latter alien to the Brazilian fauna but with which G. (O) rhyssonotum sp. n. seems to be more closely related, though the new species can be distinguished from both by a series of characteristics, mainly observed in the body shape, elytra, pronotum, and in male and female genitalia. 

Perceived Age and Perceived Physical Attractiveness

1982 ◽  
Vol 54 (3_suppl) ◽  
pp. 1251-1258 ◽  
Author(s):  
Kathleen M. Korthase ◽  
Irene Trenholme
Keyword(s):  
Physical Attractiveness ◽  
Inverse Relationship ◽  
Adolescent Females ◽  
Adolescent Males ◽  
The Other ◽  
Adult Males ◽  
Male And Female ◽  
Males And Females ◽  
Perceived Age ◽  
Female Subjects

The purpose of the present study was to determine if there exists an inverse relationship between perceived age and perceived physical attractiveness, i.e., are older faces evaluated as less attractive? Four groups of 15 subjects were studied: adult males and females (aged 31 to 38 yr., M = 34.53 yr.), adolescent males and females (aged 14 to 16 yr., M = 14.63 yr.). Subjects were given written instructions asking them to arrange two sets of photographs, male and female, according to physical attractiveness and then according to age. Perceived age and perceived physical attractiveness were negatively correlated ( rho = –.91, p < .01), i.e., as perceived age increased perceived attractiveness decreased. Differences in rankings by 30 male vs 30 female subjects and by 30 adolescent vs 30 adult subjects were not significant. However, there was greater agreement among the adolescent females than among members of the other groups for rankings of both males' attractiveness and females' age. All subjects showed greater agreement for what constitutes females' physical attractiveness than for what constitutes males' physical attractiveness.

scholarly journals Adolescent Cannabinoid and Nicotine Exposure Differentially Alters Adult Nicotine Self-Administration in Males and Females

2020 ◽  
Vol 22 (8) ◽  
pp. 1364-1373 ◽  
Author(s):  
Angeline J Dukes ◽  
James P Fowler ◽  
Valeria Lallai ◽  
Anna N Pushkin ◽  
Christie D Fowler
Keyword(s):  
Drug Exposure ◽  
Cannabinoid Receptor ◽  
Therapeutic Interventions ◽  
Adult Males ◽  
Self Administration ◽  
Male And Female ◽  
Nicotine Intake ◽  
Males And Females ◽  
The Impact ◽  
Cannabinoid Agonist

Abstract Introduction During adolescence, exposure to nicotine or cannabis independently induces effects on neuromaturation and later cognitive function. However, the potential effect of both drugs under co-use conditions has become of increasing concern given the prevalence of e-cigarettes, legalization of cannabis, and availability of synthetic “spice” cannabinoid agonists. Aims and Methods The current studies investigated the effects of exposure to a cannabinoid receptor agonist (WIN55,212-2) and/or nicotine over a discrete time period in mid-adolescence on later intravenous nicotine self-administration in adult male and female mice. We further examined whether cannabinoid agonist administration in adulthood would alter nicotine reinforcement, with either acute or chronic pairing across 7 days. Results We found that adult males exhibited increased nicotine self-administration at a lower, rewarding nicotine dose following adolescent cannabinoid exposure, either alone or with nicotine coadministration. In contrast, adult females demonstrated an opposing effect in which adolescent cannabinoid and nicotine coexposure resulted in decreased nicotine intake compared with the nicotine only and control groups. Furthermore, after maintaining nicotine self-administration across sessions, pretreatment with a low dose of the cannabinoid agonist decreased nicotine intake in both male and female control mice, and this lowering effect was evidenced after both acute and chronic treatment. However, the cannabinoid agonist was ineffective in altering nicotine intake in mice previously exposed to nicotine, cannabinoid agonist, or both during adolescence. Conclusions These data provide evidence that adolescent drug exposure can alter later nicotine reinforcement in a sex-specific manner and can further modulate the effectiveness of interventions in reducing nicotine intake during adulthood. Implications These studies demonstrate a significant impact of nicotine, cannabinoids, or coexposure on developmental processes during adolescence. Differential effects were observed within each sex, with opposing results found for cannabinoid exposure on nicotine intake in males and females. Intriguingly, we also evidenced resistance to the lowering effects of a cannabinoid agonist on nicotine intake in adulthood based on adolescent drug exposure. Thus, these findings have important implications for our understanding of the impact of nicotine and cannabinoids (eg, Δ9-tetrahydrocannabinol (THC) and synthetic “spice” cannabinoids) during development, with further implications for the effectiveness of therapeutic interventions based on prior drug exposure in youth.

Parasitism of damselflies (Enallagma boreale) by gregarines: sex biases and relations to adult survivorship

2002 ◽  
Vol 80 (1) ◽  
pp. 162-168 ◽  
Author(s):  
K R Hecker ◽  
M R Forbes ◽  
N J Léonard
Keyword(s):  
Confounding Factor ◽  
Host Age ◽  
Potential Confounding Factor ◽  
Adult Males ◽  
Intensity Of Infection ◽  
Male And Female ◽  
Adult Females ◽  
Males And Females ◽  
Enallagma Boreale ◽  
Sex Biases

We studied host damselflies Enallagma boreale (Odonata: Coenagrionidae) and their gregarine parasites (Apicomplexa: Eugregarinidae) to elucidate the causes and consequences of any sex biases in parasitism of adult hosts. Larvae of both sexes were highly infected, but there was no difference between male and female larvae in either prevalence or intensity of gregarine infections. Newly emerged adults had few or no parasites, thereby setting the stage for investigating accumulation of parasites by adults. Adult females had a higher prevalence and intensity of infection by gregarines than did males, but only on 1 (of 2) days when the potential confounding factor of host age was controlled for. Both adult males and females showed a positive correlation between longevity under conditions of food stress and the number of gregarines they initially carried. This finding may be explained if the food ingested with the infective cysts is more beneficial than the parasites are harmful, and it also has implications for investigating sex biases in numbers of trophically transmitted parasites of such insects.

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