Effect of starvation on the cold tolerance of adult Drosophila suzukii (Diptera: Drosophilidae)

Abstract The spotted wing drosophila, Drosophila suzukii, is an invasive pest in Europe and North America. Access to resources may be challenging in late fall, winter and early spring and flies may suffer from food deprivation along with cold stress in these periods. Whereas a plethora of studies have been performed on the overwintering capacity of D. suzukii, the effects of starvation on the fly's cold tolerance have not been addressed. In the present study, young D. suzukii adults (reared at 25°C, LD 12:12 h) were deprived of food for various periods (0, 12, 24 and 36 h), after which chill coma recovery time, critical thermal minimum, as well as acute and chronic cold tolerance were assessed. Additionally, the body composition of adults (body mass, water content, total lipid, glycerol, triglycerides, glucose and proteins) before and after starvation periods was analysed to confirm that starvation had detectable effects. Starved adults had a lower body mass, and both lipid and carbohydrate levels decreased with starvation time. Starvation slightly increased critical thermal minimum and affected chill coma recovery time; however, these changes were not gradual with starvation duration. Starvation promoted acute cold tolerance in both sexes. This effect appeared faster in males than in females. Food deprivation also led to enhanced survival to chronic cold stress. Short-term starvation was thus associated with significant changes in body composition in D. suzukii, and these alterations could alter some ecologically relevant traits related to cold tolerance, particularly in females. Our results suggest that food deprivation during short time (<36 h) can promote cold tolerance (especially survival after a cold stress) of D. suzukii flies. Future studies should address the ecological significance of these findings as short food deprivation may occur in the fields on many occasions and seasons.

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Artificial Selection to a Nonlethal Cold Stress in Trogoderma variabile Shows Associations With Chronic Cold Stress and Body Size

Environmental Entomology ◽

10.1093/ee/nvz162 ◽

2020 ◽

Vol 49 (2) ◽

pp. 422-434

Author(s):

Alison R Gerken ◽

Shelby R Abts ◽

Erin D Scully ◽

James F Campbell

Keyword(s):

Body Size ◽

Cold Stress ◽

Cold Tolerance ◽

Recovery Time ◽

Extreme Temperature ◽

Chill Coma ◽

Trogoderma Variabile ◽

Cold Tolerant ◽

Susceptible Populations ◽

Seven Generations

Abstract Extreme temperature has been used as an alternative to chemical treatments for stored product pests for years. Resistance to heat or cold treatments has not been documented in stored product insects, but repeated use of ineffective treatments could lead to adaptive tolerance. Trogoderma variabile (Dermestidae) is a common pest of stored products, and the larval stage is highly resistant to cold and destructive. We artificially selected populations by inducing chill coma at four different cold temperature treatments: 3 and 5 h at −10°C and 3 and 5 h at 0°C. Recovery time was highly heritable after selection for seven generations for decreased recovery time (cold tolerance) and increased recovery time (cold susceptibility) at all time and temperature combinations. Three replicate populations for each time and temperature combination varied substantially, suggesting different mutations in each population were probably responsible for selected phenotypes. Body size decreased in populations selected for cold susceptibility compared with those selected for cold tolerance and survivorship to long-term cold stress increased in the cold-tolerant populations compared with the susceptible populations. After the cessation of the selection experiment, cold tolerance dissipated within four generations from the populations at −10°C, but was maintained in populations exposed to 0°C. Our results suggest that warehouse beetles can adapt to cold stress quickly, but in the absence of cold stress, the proportion of cold-tolerant/susceptible individuals is quickly reduced, suggesting that some of the mutations responsible for these phenotypes may be associated with fitness costs under normal conditions.

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Temporal changes in body mass, body composition and metabolism of gibel carp Carassius auratus gibelio during food deprivation

Journal of Applied Ichthyology ◽

10.1111/j.1439-0426.2007.00827.x ◽

2007 ◽

Vol 23 (3) ◽

pp. 215-220 ◽

Cited By ~ 6

Author(s):

Z. Cui ◽

Y. Wang

Keyword(s):

Body Composition ◽

Body Mass ◽

Food Deprivation ◽

Carassius Auratus ◽

Temporal Changes ◽

Gibel Carp ◽

Carassius Auratus Gibelio

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Ovary Development and Cold Tolerance of the Invasive Pest Drosophila suzukii (Matsumura) in the Central Plains of Kansas, United States

Environmental Entomology ◽

10.1093/ee/nvy074 ◽

2018 ◽

Vol 47 (4) ◽

pp. 1013-1023 ◽

Cited By ~ 12

Author(s):

E R Everman ◽

P J Freda ◽

M Brown ◽

A J Schieferecke ◽

G J Ragland ◽

...

Keyword(s):

United States ◽

Cold Tolerance ◽

Invasive Pest ◽

Ovary Development ◽

Drosophila Suzukii ◽

Central Plains

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Insect capa neuropeptides impact desiccation and cold tolerance

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1501518112 ◽

2015 ◽

Vol 112 (9) ◽

pp. 2882-2887 ◽

Cited By ~ 71

Author(s):

Selim Terhzaz ◽

Nicholas M. Teets ◽

Pablo Cabrero ◽

Louise Henderson ◽

Michael G. Ritchie ◽

...

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Cold Tolerance ◽

Physiological Mechanism ◽

Neuroendocrine System ◽

Renal Tubules ◽

Chill Coma ◽

Peptide Analogs ◽

Dipteran Species

The success of insects is linked to their impressive tolerance to environmental stress, but little is known about how such responses are mediated by the neuroendocrine system. Here we show that the capability (capa) neuropeptide gene is a desiccation- and cold stress-responsive gene in diverse dipteran species. Using targeted in vivo gene silencing, physiological manipulations, stress-tolerance assays, and rationally designed neuropeptide analogs, we demonstrate that the Drosophila melanogaster capa neuropeptide gene and its encoded peptides alter desiccation and cold tolerance. Knockdown of the capa gene increases desiccation tolerance but lengthens chill coma recovery time, and injection of capa peptide analogs can reverse both phenotypes. Immunohistochemical staining suggests that capa accumulates in the capa-expressing Va neurons during desiccation and nonlethal cold stress but is not released until recovery from each stress. Our results also suggest that regulation of cellular ion and water homeostasis mediated by capa peptide signaling in the insect Malpighian (renal) tubules is a key physiological mechanism during recovery from desiccation and cold stress. This work augments our understanding of how stress tolerance is mediated by neuroendocrine signaling and illustrates the use of rationally designed peptide analogs as agents for disrupting protective stress tolerance.

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Multiple paths to cold tolerance: the role of environmental cues, morphological traits and the circadian clock gene vrille

BMC Ecology and Evolution ◽

10.1186/s12862-021-01849-y ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Noora Poikela ◽

Venera Tyukmaeva ◽

Anneli Hoikkala ◽

Maaria Kankare

Keyword(s):

Circadian Clock ◽

Cold Acclimation ◽

Cold Tolerance ◽

Recovery Time ◽

Morphological Traits ◽

Clock Gene ◽

Circadian Clock Gene ◽

Chill Coma ◽

The Impact ◽

Insect Cold Tolerance

Abstract Background Tracing the association between insect cold tolerance and latitudinally and locally varying environmental conditions, as well as key morphological traits and molecular mechanisms, is essential for understanding the processes involved in adaptation. We explored these issues in two closely-related species, Drosophila montana and Drosophila flavomontana, originating from diverse climatic locations across several latitudes on the coastal and mountainous regions of North America. We also investigated the association between sequence variation in one of the key circadian clock genes, vrille, and cold tolerance in both species. Finally, we studied the impact of vrille on fly cold tolerance and cold acclimation ability by silencing it with RNA interference in D. montana. Results We performed a principal component analysis (PCA) on variables representing bioclimatic conditions on the study sites and used latitude as a proxy of photoperiod. PC1 separated the mountainous continental sites from the coastal ones based on temperature variability and precipitation, while PC2 arranged the sites based on summer and annual mean temperatures. Cold tolerance tests showed D. montana to be more cold-tolerant than D. flavomontana and chill coma resistance (CTmin) of this species showed an association with PC2. Chill coma recovery time (CCRT) of both species improved towards northern latitudes, and in D. flavomontana this trait was also associated with PC1. D. flavomontana flies were darkest in the coast and in the northern mountainous populations, but coloration showed no linkage with cold tolerance. Body size decreased towards cold environments in both species, but only within D. montana populations largest flies showed fastest recovery from cold. Finally, both the sequence analysis and RNAi study on vrille suggested this gene to play an essential role in D. montana cold resistance and acclimation, but not in recovery time. Conclusions Our study demonstrates the complexity of insect cold tolerance and emphasizes the need to trace its association with multiple environmental variables and morphological traits to identify potential agents of natural selection. It also shows that a circadian clock gene vrille is essential both for short- and long-term cold acclimation, potentially elucidating the connection between circadian clock system and cold tolerance.

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Three quantitative trait loci explain more than 60% of phenotypic variation for chill coma recovery time in Drosophila ananassae

10.1101/676262 ◽

2019 ◽

Author(s):

Annabella Königer ◽

Saad Arif ◽

Sonja Grath

Keyword(s):

Candidate Genes ◽

Cold Tolerance ◽

Quantitative Trait ◽

Recovery Time ◽

Differentially Expressed ◽

Drosophila Ananassae ◽

Phenotypic Variance ◽

Climatic Fluctuations ◽

Standard Interval ◽

Chill Coma

AbstractEctothermic species such as insects are particularly vulnerable to climatic fluctuations. Nevertheless, many insects that evolved and diversified in the tropics have successfully colonized temperate regions all over the globe. To shed light on the genetic basis of cold tolerance in such species, we conducted a quantitative trait locus (QTL) mapping experiment for chill coma recovery time (CCRT) in Drosophila ananassae, a cosmopolitan species that has expanded its range from tropical to temperate regions.We created a mapping population of recombinant inbred advanced intercross lines (RIAILs) from two founder strains with diverging CCRT phenotypes. The RIAILs were phenotyped for their CCRT and, together with the founder strains, genotyped for polymorphic markers with double-digest restriction site-associated DNA (ddRAD) sequencing. Using a hierarchical mapping approach that combined standard interval mapping and a multiple-QTL model, we mapped three QTL which altogether explained 64% of the phenotypic variance. For two of the identified QTL, we found evidence of epistasis. To narrow down the list of cold tolerance candidate genes, we cross-referenced the QTL intervals with genes that we previously identified as differentially expressed in response to cold in D. ananassae, and with thermotolerance candidate genes of D. melanogaster. Among the 58 differentially expressed genes that were contained within the QTL, GF15058 showed a significant interaction of the CCRT phenotype and gene expression. Further, we identified the orthologs of four D. melanogaster thermotolerance candidate genes, MtnA, klarsicht, CG5246 (D.ana/GF17132) and CG10383 (D.ana/GF14829) as candidates for cold tolerance in D. ananassae.

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A Comparative Analysis on Body Composition, Risk Factors of Metabolic Disease, and Arteriosclerosis According to the Body Mass Index of Female College Students

Korean Journal of Sports Science ◽

10.35159/kjss.2018.12.27.6.939 ◽

2018 ◽

Vol 27 (6) ◽

pp. 939-947

Author(s):

Chul-Soon Choi

Keyword(s):

Risk Factors ◽

College Students ◽

Metabolic Disease ◽

Body Mass Index ◽

Body Composition ◽

Comparative Analysis ◽

Body Mass ◽

The Body ◽

Female College Students ◽

Female College

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Comparison of the Effect of Soy protein and Whey protein on Body Composition: A Meta-Analysis of Randomized Clinical Trials

British Journal Of Nutrition ◽

10.1017/s0007114521001550 ◽

2021 ◽

pp. 1-27

Author(s):

Masoome Piri Damaghi ◽

Atieh Mirzababaei ◽

Sajjad Moradi ◽

Elnaz Daneshzad ◽

Atefeh Tavakoli ◽

...

Keyword(s):

Body Composition ◽

Whey Protein ◽

Lean Body Mass ◽

Body Mass ◽

Body Fat ◽

Fat Mass ◽

Soy Protein ◽

Protein Supplementation ◽

Body Fat Percentage ◽

Fat Percentage

Abstract Background: Essential amino acids (EAAs) promote the process of regulating muscle synthesis. Thus, whey protein that contains higher amounts of EAA can have a considerable effect on modifying muscle synthesis. However, there is insufficient evidence regarding the effect of soy and whey protein supplementation on body composition. Thus, we sought to perform a meta-analysis of published Randomized Clinical Trials that examined the effect of whey protein supplementation and soy protein supplementation on body composition (lean body mass, fat mass, body mass and body fat percentage) in adults. Methods: We searched PubMed, Scopus, and Google Scholar, up to August 2020, for all relevant published articles assessing soy protein supplementation and whey protein supplementation on body composition parameters. We included all Randomized Clinical Trials that investigated the effect of whey protein supplementation and soy protein supplementation on body composition in adults. Pooled means and standard deviations (SD) were calculated using random-effects models. Subgroup analysis was applied to discern possible sources of heterogeneity. Results: After excluding non-relevant articles, 10 studies, with 596 participants, remained in this study. We found a significant increase in lean body mass after whey protein supplementation weighted mean difference (WMD: 0.91; 95% CI: 0.15, 1.67. P= 0.019). Subgroup analysis, for whey protein, indicated that there was a significant increase in lean body mass in individuals concomitant to exercise (WMD: 1.24; 95% CI: 0.47, 2.00; P= 0.001). There was a significant increase in lean body mass in individuals who received 12 or less weeks of whey protein (WMD: 1.91; 95% CI: 1.18, 2.63; P<0.0001). We observed no significant change between whey protein supplementation and body mass, fat mass, and body fat percentage. We found no significant change between soy protein supplementation and lean body mass, body mass, fat mass, and body fat percentage. Subgroup analysis for soy protein indicated there was a significant increase in lean body mass in individuals who supplemented for 12 or less weeks with soy protein (WMD: 1.48; 95% CI: 1.07, 1.89; P< 0.0001). Conclusion: Whey protein supplementation significantly improved body composition via increases in lean body mass, without influencing fat mass, body mass, and body fat percentage.

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Changes in Children’s Body Composition and Posture during Puberty Growth

Children ◽

10.3390/children8040288 ◽

2021 ◽

Vol 8 (4) ◽

pp. 288

Author(s):

Wojciech Rusek ◽

Joanna Baran ◽

Justyna Leszczak ◽

Marzena Adamczyk ◽

Rafał Baran ◽

...

Keyword(s):

Body Composition ◽

Adipose Tissue ◽

Regression Analysis ◽

Body Mass ◽

Pelvic Obliquity ◽

The Body ◽

Body Posture ◽

Older Children ◽

The Difference ◽

The Right

The main goal of our study was to determine how the age of children, puberty and anthropometric parameters affect the formation of body composition and faulty body posture development in children. The secondary goal was to determine in which body segments abnormalities most often occur and how gender differentiates the occurrence of adverse changes in children’s body posture and body composition during puberty. The study group consisted of 464 schoolchildren aged from 6–16. Body posture was assessed with the Zebris system. The composition of the body mass was tested with Tanita MC 780 MA body mass analyzer and the body height was measured using a portable stadiometer PORTSTAND 210. The participants were further divided due to the age of puberty. Tanner division was adopted. The cut-off age for girls is ≥10 years and for boys it is ≥12 years. The analyses applied descriptive statistics, the Pearson correlation, stepwise regression analysis and the t-test. The accepted level of significance was p < 0.05. The pelvic obliquity was lower in older children (beta = −0.15). We also see that age played a significant role in the difference in the height of the right pelvis (beta = −0.28), and the difference in the height of the right shoulder (beta = 0.23). Regression analysis showed that the content of adipose tissue (FAT%) increased with body mass index (BMI) and decreased with increasing weight, age, and height. Moreover, the FAT% was lower in boys than in girls (beta negative equal to −0.39). It turned out that older children (puberty), had greater asymmetry in the right shoulder blade (p < 0.001) and right shoulder (p = 0.003). On the other hand, younger children (who were still before puberty) had greater anomalies in the left trunk inclination (p = 0.048) as well as in the pelvic obliquity (p = 0.008). Girls in puberty were characterized by greater asymmetry on the right side, including the shoulders (p = 0.001), the scapula (p = 0.001) and the pelvis (p < 0.001). In boys, the problem related only to the asymmetry of the shoulder blades (p < 0.001). Girls were characterized by a greater increase in adipose tissue and boys by muscle tissue. Significant differences also appeared in the body posture of the examined children. Greater asymmetry within scapulas and shoulders were seen in children during puberty. Therefore, a growing child should be closely monitored to protect them from the adverse consequences of poor posture or excessive accumulation of adipose tissue in the body.

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