The N-acetyl neuraminic-acid content of the milk of various species

1973 ◽  
Vol 40 (3) ◽  
pp. 421-425 ◽  
Author(s):  
P. A. Morrissey

SummaryAverage N-acetyl neuraminic-acid (NANA) content of milk from cow, sheep, goat, mare, donkey and sow (about 10 samples from each species) was 17·7, 10·1, 6·4, 6·1, 12·0 and 64·0mg/100 ml, of which 14·2, 8·4, 3·2, 3·1, 5·0 and 53·7mg/100 ml respectively was in the casein fraction. The NANA content of casein showed marked species variation ranging from an average of 2·18 % for the sow to 0·09 % for the goat.About 28 % of the casein NANA of mare's milk was liberated in the fraction soluble in 12 % TCA by the action of rennin at pH 6·6 compared with 75 and around 100 % for donkey's and sow's milk respectively; about half the total NANA in the casein of cow's, sheep's and goat's milk was released under the same conditions.

1953 ◽  
Vol 36 (1) ◽  
pp. 29-32 ◽  
Author(s):  
R.A. Collins ◽  
R.E. Boldt ◽  
C.A. Elvehjem ◽  
E.B. Hart

2010 ◽  
Vol 31 (13) ◽  
pp. 2211-2217 ◽  
Author(s):  
Azahara Carpio ◽  
Vicente Rodríguez-Estévez ◽  
Manuel Sánchez-Rodríguez ◽  
Lourdes Arce ◽  
Miguel Valcárcel

2011 ◽  
Vol 92 (8) ◽  
pp. 1657-1664 ◽  
Author(s):  
Adela Abellán ◽  
José María Cayuela ◽  
Antonio Pino ◽  
Adela Martínez-Cachá ◽  
Eva Salazar ◽  
...  

1940 ◽  
Vol 20 (2) ◽  
pp. 99-108
Author(s):  
Martha S. Richmond ◽  
G. H. Satterfield ◽  
C. D. Grinnells ◽  
W. J. Dann

2019 ◽  
Vol 43 (1) ◽  
pp. 50-58
Author(s):  
H. S. Alnaemi

     Fate of AflatoxinM1 in soft white cheese and its by-product (whey) and in yogurt locally made from raw sheep's and goat's milk experimentally inoculated with 0.05 and 0.5 µg/l AflatoxinM1 were investigated using ELISA technique. Results reported that AflatoxinM1 was concentrated in cheese at levels significantly higher than that recorded in the raw milk that used for its processing, with a significant decrease in AflatoxinM1 levels in its by-product (whey) comparable to the raw milk used in manufacturing at both inoculated levels. Yogurt produced from raw sheep's milk at second inoculated level exerted AflatoxinM1concentration significantly lower than that present in the milk. Significant differences in AflatoxinM1distribution in cheese and whey produced from sheep's milk comparable to their counterparts produced from goat's milk were recorded. Finally, results revealed the efficacious role of the various dairy manufacturing processes in AflatoxinM1 distribution and the necessity to issue of local legislations concerning the maximum permissible limits for AflatoxinM1 in milk in order to stay within the universal permissible levels for AflatoxinM1 in dairy products to provide greater protection for consumer health. 


2021 ◽  
pp. 108201322110037
Author(s):  
Ercan Sarica ◽  
Hayri Coşkun

This study was aimed to determine the changes in kefir samples (CK and GK) made from cow’s and goat’s milk during frozen storage. The CK and GK samples were first stored at +4 °C for 14 and 21 days. Thereafter, all the samples were frozen at –35 °C for 24 h and kept at –18 °C for 45 days. There was no significant change in the fat, protein, acidity and pH values in both samples during the storage. The values of viscosity, WI and C* were higher in the CK samples while the syneresis value was higher in the GK samples throughout the frozen storage. The microorganisms ( Lactococcus spp., Lactobacillus spp., Leuconostoc spp., total mesophilic aerobic bacteria and yeasts) found in kefir made from goat's milk were more affected from the frozen storage. In both samples, the changes in organic acids and volatile flavor components were not significant during frozen storage, except acetic, citric and oxalic acids and acetaldehyde in GK sample. In addition, CK samples were preferred sensorially more by the panellists during frozen storage.


Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2625
Author(s):  
Muzammeer Mansor ◽  
Jameel R. Al-Obaidi ◽  
Nurain Nadiah Jaafar ◽  
Intan Hakimah Ismail ◽  
Atiqah Farah Zakaria ◽  
...  

Two-dimensional electrophoretic (2DE)-based proteomics remains a powerful tool for allergenomic analysis of goat’s milk but requires effective extraction of proteins to accurately profile the overall causative allergens. However, there are several current issues with goat’s milk allergenomic analysis, and among these are the absence of established standardized extraction method for goat’s milk proteomes and the complexity of goat’s milk matrix that may hamper the efficacy of protein extraction. This study aimed to evaluate the efficacies of three different protein extraction methods, qualitatively and quantitatively, for the 2DE-proteomics, using milk from two commercial dairy goats in Malaysia, Saanen, and Jamnapari. Goat’s milk samples from both breeds were extracted by using three different methods: a milk dilution in urea/thiourea based buffer (Method A), a triphasic separation protocol in methanol/chloroform solution (Method B), and a dilution in sulfite-based buffer (Method C). The efficacies of the extraction methods were assessed further by performing the protein concentration assay and 1D and 2D SDS-PAGE profiling, as well as identifying proteins by MALDI-TOF/TOF MS/MS. The results showed that method A recovered the highest amount of proteins (72.68% for Saanen and 71.25% for Jamnapari) and produced the highest number of protein spots (199 ± 16.1 and 267 ± 10.6 total spots for Saanen and Jamnapari, respectively) with superior gel resolution and minimal streaking. Six milk protein spots from both breeds were identified based on the positive peptide mass fingerprinting matches with ruminant milk proteins from public databases, using the Mascot software. These results attest to the fitness of the optimized protein extraction protocol, method A, for 2DE proteomic and future allergenomic analysis of the goat’s milk.


2013 ◽  
Vol 64 (3) ◽  
pp. 304-310 ◽  
Author(s):  
P. Hueso ◽  
L. Zancada ◽  
F. Pérez-Díez ◽  
F. Sánchez-Juanes ◽  
J. M. Alonso ◽  
...  

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