Mucosal lesions in the rat associated with the attachment ofMoniliformis(Acanthocephala)

1983 ◽  
Vol 57 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Jean Martin ◽  
D. W. T. Crompton ◽  
Aruna Singhvi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Goblet Cells ◽  
Intestinal Wall ◽  
Anterior Region ◽  
Mucosal Tissue ◽  
Mucosal Lesions ◽  
Scanning Electron

ABSTRACTBy means of scanning electron microscopy, aspects of the attachment ofMoniliformis dubius(Acanthocephala) to the intestinal wall of the rat are described together with various changes in the structure and form of the mucosa. Attachment of the parasite results in the formation of a distinct pit around the everted proboscis and a crater around the neck. Contact between the anterior region of the trunk of the worm and the intestine of the host appears to lead to villous fusion so that characteristic grooves are formed in the mucosal tissue. Evidence was also obtained to suggest that host goblet cells may become exhausted during the course of an infection withMoniliformis.

Scanning electron microscopy of the operculum of Garra lamta (Hamilton) (Cyprinidae:Cypriniformes), an Indian hill stream fish

2010 ◽  
Vol 58 (3) ◽  
pp. 182 ◽  
Author(s):  
Swati Mittal ◽  
Usha Kumari ◽  
Pinky Tripathi ◽  
Ajay Kumar Mittal
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Epithelial Cells ◽  
Goblet Cells ◽  
Stream Fish ◽  
Male And Female ◽  
Fish Breeding ◽  
Inner Surface ◽  
Garra Lamta ◽  
Scanning Electron

The surface architecture of the epidermis on the outer surface of the operculum (OE) and the epithelium on the inner surface of the operculum (EISO) of Garra lamta was examined by scanning electron microscopy. The surface appeared smooth on the OE and wavy on the EISO. A wavy epithelium is considered to facilitate an increase in its stretchability, during the expansion of the branchial chamber. The OE and the EISO were covered by a mosaic pavement of epithelial cells with characteristic patterns of microridges and microbridges. Interspersed between the epithelial cells were mucous goblet cell pores, which were not significantly different in number in the OE and the EISO. Nevertheless, their surface area in the EISO was significantly higher than in the OE. This could be an adaptation to secrete higher amounts of mucus on the EISO for keeping the branchial chamber lining clean, avoiding clogging, the increased slipperiness reducing friction from water flow and increased efficiency in protecting against microbial attachments. Rounded bulges on the OE and the EISO were associated with mucous goblet cells. The absence of the taste buds in the EISO, in contrast to the OE, suggests that their function in the branchial chamber may not be of much significance in this fish. Breeding tubercles on the OE are believed to facilitate better contact between the male and female during breeding.

Study of the nasopharynx in man by scanning electron microscopy

1988 ◽  
Vol 102 (12) ◽  
pp. 1102-1106 ◽  
Author(s):  
M. Gulisano ◽  
G. Polli ◽  
G. Biondi ◽  
P. Pacini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Healthy Volunteers ◽  
Squamous Epithelium ◽  
Goblet Cells ◽  
Transitional Epithelium ◽  
Ciliated Cells ◽  
Scanning Electron

AbstractTo date only few and often disagreeing studies about human nasopharynx are available.The present research has the purpose to give a contribution to the knowledge of nasopharyngeal epithelium using S.E.M The study was carried out on biopsies taken from 20 healthy volunteers.The surface of nasopharynx is covered by ciliated cells, microvilli provided cells and goblet cells.Areas covered with squamous epithelium and presumably transitional epithelium were observed.The possibility that ciliated become microvilli provided cells is discussed.

Ultrastructure of the egg of Coquillettidia venezuelensis (Theobald) (Diptera: Culicidae)

Zootaxa ◽  
2020 ◽  
Vol 4894 (3) ◽  
pp. 432-436
Author(s):  
CECILIA FERREIRA DE MELLO ◽  
JERONIMO ALENCAR
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Central Region ◽  
Outer Margin ◽  
Anterior Region ◽  
Width Ratio ◽  
Ultrastructural Characteristics ◽  
Scanning Electron

This study examined the ultrastructural characteristics of the egg of Coquillettidia venezuelensis (Theobald) (Diptera: Culicidae) with the aid of scanning electron microscopy. The eggs are elliptical and blackish, measuring on average 525.3 ± 12.8 μm in length and 94.9 ± 5.14 μm in width, with a length to width ratio of 5.54 ± 0.18. The anterior region of the egg is tubuliform, with a well-developed micropylar collar measuring approximately 2.54 ± 0.33 μm in thickness, the inner side of which is uniformly and deeply excavated, with a depression around the outer margin of the micropylar disc, which has a diameter of 29.4 ± 1.7 μm, an area of 234.4 μm2 ± 401.3 μm2 and a slightly elevated central region measuring 1.7 ± 0.5 μm in diameter that bears the central micropyle. 

Nematodirus battus: scanning electron microscope studies of the duodenal mucosa of infected lambs

Parasitology ◽  
1980 ◽  
Vol 81 (3) ◽  
pp. 573-578 ◽  
Author(s):  
Jean Martin ◽  
D. L. Lee
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Goblet Cells ◽  
Duodenal Mucosa ◽  
Intestinal Damage ◽  
Third Stage ◽  
Scanning Electron

SUMMARYLambs given 60000 third-stage larvae of Nematodirus battus were killed on days 16, 20, 22, 24 and 32 of the infection and the duodenal mucosa was examined by scanning electron microscopy (SEM). On day 16 of the infection the villi were long and finger-like and, although goblet cells were visible, the surface of the villi was not extensively folded, as is the surface of villi of uninfected control animals. By day 20 of the infection the villi had been reduced to flattened, plate-like structures or had formed low, irregular-shaped ridges. On this day of the infection the nematodes appeared to be enclosed by mucus-like material. As the infection progressed the intestinal damage was repaired, so that, by day 24 of the infection, the surface of the intestine was covered by short, smooth, finger-like projections and, by day 32 of the infection, the morphology of the intestine was somewhat similar to that of the control lamb. The possible role of villus atrophy and of mucus in the rejection of N. battus from the intestine is discussed.

Scanning Electron Microscopy of Experimental Keratoconjunctivitis Sicca in Dogs: Cornea and Bulbar Conjunctiva

1988 ◽  
Vol 25 (6) ◽  
pp. 468-474 ◽  
Author(s):  
T. J. Kern ◽  
H. N. Erb ◽  
J. M. Schaedler ◽  
E. P. Dougherty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Replacement Therapy ◽  
Cell Density ◽  
Goblet Cell ◽  
Keratoconjunctivitis Sicca ◽  
Goblet Cells ◽  
Dark Cell ◽  
Scanning Electron ◽  
Goblet Cell Density

Keratoconjunctivitis sicca was produced experimentally in 16 beagles by bilateral surgical removal of the lacrimal and nictitans glands; four dogs were not treated, and 12 received tear-replacement therapy on post-operative days 7 through 28. Keratoconjunctivitis sicca was verified by reduction in Schirmer tear test values by post-operative day 6, and there was no response on day 28 to tear-replacement therapy. Corneas of both normal and tear-deficient dogs had polygonal squamous epithelial cells of light and dark electron density by scanning electron microscopy. Light cells had more microvilli and microplicae than dark cells. Conjunctivae were similar to corneas, except for numerous goblet cells on the surface. Corneal dark-cell density and goblet cell density were not different between groups. Goblet cells most often occurred singly in normal dogs, while they were in clusters in tear-deficient dogs. A hypothesis that petrolatum/mineral oil ointment should provide more effective artificial tear replacement than hydroxymethylcellulose drops for tear-deficient dogs could not be confirmed by objective analysis of corneal dark-cell density or conjunctival goblet cell density.

Surface changes in the intestinal mucosa of mice infected with Eimeria falciformis

1984 ◽  
Vol 62 (9) ◽  
pp. 1675-1679 ◽  
Author(s):  
Clarence A. Speer ◽  
Franklin L. Pollari
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basal Lamina ◽  
Intestinal Mucosa ◽  
Goblet Cells ◽  
Mucosal Surface ◽  
Apical Area ◽  
Absorptive Enterocytes ◽  
Scanning Electron ◽  
Surface Changes

Scanning electron microscopy (SEM) was used to study changes in the mucosa of the cecum and colon of mice infected with Eimeria falciformis. Pieces of the cecum and colon were removed from mice that had been inoculated 1–12 days earlier with 106 or 2 × 104 sporulated oocysts, and then processed for SEM. Alterations of the mucosal surface of infected mice were restricted to the cecum and upper half of the colon. At 2–8 days postinoculation (DPI), the apical area of absorptive enterocytes gradually increased to more than 4 times normal, and goblet cells initially increased in number, then declined and eventually disappeared. Lesions in the epithelium which exposed the basal lamina were most severe at 6–8 DPI. Repair of the epithelium occurred at 9–12 DPI.

scholarly journals PATHOLOGICAL STUDY OF LOCALLY ISOLATED FOWL POXVIRUS -EXPERIMENTALLY INFECTED TRACHEA USING SCANNING ELECTRON MICROSCOPY

2021 ◽  
Vol 26 (1) ◽  
pp. 109-117
Author(s):  
H. M. Al-Hyali ◽  
A. Al-Jumily
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Epithelial Cells ◽  
Respiratory Distress ◽  
Goblet Cells ◽  
Post Inoculation ◽  
Pathological Study ◽  
Tracheal Mucosa ◽  
Epithelial Surface ◽  
Scanning Electron

In this study fowl poxvirus has been isolated from naturally infected birds. This virus induced respiratory distress, 2, 3 and 4 days post inoculation, in intratracheally inoculated 8 week old chickens, these respiratory distress include congestion of tracheal mucosa, increased tracheal secreation and whitish-yellowish pastules appeared at ninth day post inoculation in the upper part of the trachea forming diphtheritic or pseudodiphtheritic membrane. Using scanning electron microscopy to study the changes that occurs in the epithelial surface of trachea. It was found that the virus causes proliferation of epithelial cells forming cluster like papilloma, deciliation and activation of goblet cells.

scholarly journals Morphology of Intestinal Goblet Cells of The Dromedary (Camelus dromedarius)

1970 ◽  
Vol 8 (1) ◽  
pp. 1379-1384
Author(s):  
AMA Mohamed ◽  
AAM Taha ◽  
AM Ali
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Goblet Cells ◽  
Camelus Dromedarius ◽  
Electron Microscopes ◽  
Columnar Cells ◽  
Mucosal Fold ◽  
Scanning Electron ◽  
Large Spherical

Morphology of the goblet cells in the intestine of the dromedary (Camelus dromedarius ) was carried out by light and electron microscopes. Histologically, the goblet cells appeared as globular or ovoidshaped with basally located flat nuclei. They were dispersed among the columnar cells lining the epithelia of the villi and crypts of Lieberkuhn, and they increased greatly in number towards the rectum. Goblet cells showed positive reaction to (PAS) stain. Ultrastructurally, the scanning electron microscopy showed villi with rough surface which displayed goblet cells and microvilli in the small intestine. In the large intestine, the surface of the mucosal fold was characteristically covered by goblet cells and epithelial cells. The ultrastructure of the goblet cells showed the cell membrane lacked microvilli and the cytoplasm contained numerous large spherical granules of mucin, rough endoplasmic reticulum and mitochondria. The nucleus containing eccentric nucleolus, chromatin and heterochromatin materials.Key words: dromedary, goblet cells, intestine, morphology

Histomorphology and scanning electron microscopy of the pharyngeal tonsil in goats

2016 ◽  
Author(s):  
V. R. Indu ◽  
K. M. Lucy ◽  
N. Ashok ◽  
S. Maya ◽  
V. L. Gleeja
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Lymphoid Tissue ◽  
Average Diameter ◽  
Goblet Cells ◽  
Surface Epithelium ◽  
Blood Capillaries ◽  
Follicle Associated Epithelium ◽  
Scanning Electron ◽  
Pharyngeal Tonsil

Gross and histological studies were conducted on the pharyngeal tonsil of six male crossbred goats of six months of age. In the nasopharynx, pharyngeal tonsil was located on the caudal part of the pharyngeal septum and was 5.54±1.41cm long and 2.19±0.92cm wide. It presented numerous longitudinally arranged primary and secondary folds. Histologically the tonsil was lined by pseudostratified ciliated columnar epithelium comprising of 8-14 rows of nuclei of three types of cells, viz. basal, supporting and goblet cells. This epithelium was transformed at places into follicle-associated epithelium (FAE) and was characterized by decreased height of the epithelial cells, absence of cilia and goblet cells and heavy infiltration of lymphocytes through the interrupted basement membrane. The height of surface epithelium was 87.33± 1.20μm and that of follicle-associated epithelium was 52.33± 5.21μm. Propria-submucosa comprised of a central axis of loosely arranged connective tissue with dense aggregates of lymphoid tissue, fine blood capillaries and few nerve fibres folded around it. The cryptolymphatic units and tonsillar nodules of varying shape and dimensions constituted the majority of the lymphoid tissue. The average diameter of lymphoid nodules was 921.67±8.72μm and the lymphocyte count per nodule was 32233.23±324.24. The average number of lymphatic nodules counted per field under low power magnification of microscope was 2.5±0.43 and the internodular distance was 29.83±1.40μm. In scanning electron microscopy surface of the pharyngeal tonsil was covered by two types of epithelium viz., the ciliated respiratory surface epithelium and the FAE consisting predominantly of three types of non-ciliated microvillus cells.

Description of the egg of Hulecoeteomyia koreica (Edwards) (Diptera: Culicidae) using scanning electron microscopy

Zootaxa ◽  
2018 ◽  
Vol 4442 (1) ◽  
pp. 194-200
Author(s):  
CARLOS BRISOLA MARCONDES ◽  
CECÍLIA FERREIRA DE MELLO ◽  
AMANDA QUEIROZ BASTOS ◽  
FABRIZIO MONTARSI ◽  
JERONIMO ALENCAR ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Mosquito Species ◽  
Anterior Region ◽  
Aedes Koreicus ◽  
Invasive Mosquito ◽  
Scanning Electron

The egg of Hulecoeteomyia koreica (Edwards) [=Aedes koreicus (Edwards)], an invasive mosquito in Europe, is described from material obtained in Italy. Eggs are cigar-shaped and tapered posteriorly. They are 667.5 ±47.27 µm in length, and the width posteriorly, in the middle and near the micropyle is, respectively, 113.18 ±10.07, 182.5 ±26.92 and 142.25 ±13.85µm. The outer chorion has groups of tubercles, 3 or 4 in an irregular row in the anterior region and 4–6 irregularly dispersed in cavities surrounded by a covering similar to a membrane. The micropyle is surrounded by an irregular collar, and groups of tubercles are less defined in this region. This pattern is different from the egg described for any other mosquito species. 

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