Histomorphology and scanning electron microscopy of the pharyngeal tonsil in goats

Author(s):  
V. R. Indu ◽  
K. M. Lucy ◽  
N. Ashok ◽  
S. Maya ◽  
V. L. Gleeja

Gross and histological studies were conducted on the pharyngeal tonsil of six male crossbred goats of six months of age. In the nasopharynx, pharyngeal tonsil was located on the caudal part of the pharyngeal septum and was 5.54±1.41cm long and 2.19±0.92cm wide. It presented numerous longitudinally arranged primary and secondary folds. Histologically the tonsil was lined by pseudostratified ciliated columnar epithelium comprising of 8-14 rows of nuclei of three types of cells, viz. basal, supporting and goblet cells. This epithelium was transformed at places into follicle-associated epithelium (FAE) and was characterized by decreased height of the epithelial cells, absence of cilia and goblet cells and heavy infiltration of lymphocytes through the interrupted basement membrane. The height of surface epithelium was 87.33± 1.20μm and that of follicle-associated epithelium was 52.33± 5.21μm. Propria-submucosa comprised of a central axis of loosely arranged connective tissue with dense aggregates of lymphoid tissue, fine blood capillaries and few nerve fibres folded around it. The cryptolymphatic units and tonsillar nodules of varying shape and dimensions constituted the majority of the lymphoid tissue. The average diameter of lymphoid nodules was 921.67±8.72μm and the lymphocyte count per nodule was 32233.23±324.24. The average number of lymphatic nodules counted per field under low power magnification of microscope was 2.5±0.43 and the internodular distance was 29.83±1.40μm. In scanning electron microscopy surface of the pharyngeal tonsil was covered by two types of epithelium viz., the ciliated respiratory surface epithelium and the FAE consisting predominantly of three types of non-ciliated microvillus cells.

2018 ◽  
Vol 3 (1) ◽  
pp. 12 ◽  
Author(s):  
Zaimahwati Zaimahwati ◽  
Yuniati Yuniati ◽  
Ramzi Jalal ◽  
Syahman Zhafiri ◽  
Yuli Yetri

<p>Pada penelitian ini telah dilakukan isolasi dan karakterisasi bentonit alam menjadi nanopartikel montmorillonit. Bentonit alam yang digunakan diambil dari desa Blangdalam, Kecamatan Nisam Kabupaten Aceh Utara.  Proses isolasi meliputi proses pelarutan dengan aquades, ultrasonic dan proses sedimentasi. Untuk mengetahui karakterisasi montmorillonit dilakukan uji FT-IR, X-RD dan uji morfologi permukaan dengan Scanning Electron Microscopy (SEM). Partikel size analyzer untuk menganalisis dan menentukan ukuran nanopartikel dari isolasi bentonit alam. Dari hasil penelitian didapat ukuran nanopartikel montmorillonit hasil isolasi dari bentonit alam diperoleh berdiameter rata-rata 82,15 nm.</p><p><em>In this research we have isolated and characterized natural bentonite into montmorillonite nanoparticles. Natural bentonite used was taken from Blangdalam village, Nisam sub-district, North Aceh district. The isolation process includes dissolving process with aquades, ultrasonic and sedimentation processes.  The characterization of montmorillonite, FT-IR, X-RD and surface morphology test by Scanning Electron Microscopy (SEM). Particle size analyzer to analyze and determine the size of nanoparticles from natural bentonite insulation. From the research results obtained the size of montmorillonite nanoparticles isolated from natural bentonite obtained an average diameter of 82.15 nm.</em></p>


2010 ◽  
Vol 58 (3) ◽  
pp. 182 ◽  
Author(s):  
Swati Mittal ◽  
Usha Kumari ◽  
Pinky Tripathi ◽  
Ajay Kumar Mittal

The surface architecture of the epidermis on the outer surface of the operculum (OE) and the epithelium on the inner surface of the operculum (EISO) of Garra lamta was examined by scanning electron microscopy. The surface appeared smooth on the OE and wavy on the EISO. A wavy epithelium is considered to facilitate an increase in its stretchability, during the expansion of the branchial chamber. The OE and the EISO were covered by a mosaic pavement of epithelial cells with characteristic patterns of microridges and microbridges. Interspersed between the epithelial cells were mucous goblet cell pores, which were not significantly different in number in the OE and the EISO. Nevertheless, their surface area in the EISO was significantly higher than in the OE. This could be an adaptation to secrete higher amounts of mucus on the EISO for keeping the branchial chamber lining clean, avoiding clogging, the increased slipperiness reducing friction from water flow and increased efficiency in protecting against microbial attachments. Rounded bulges on the OE and the EISO were associated with mucous goblet cells. The absence of the taste buds in the EISO, in contrast to the OE, suggests that their function in the branchial chamber may not be of much significance in this fish. Breeding tubercles on the OE are believed to facilitate better contact between the male and female during breeding.


2013 ◽  
Vol 334-335 ◽  
pp. 60-64 ◽  
Author(s):  
Mohammad Reza Loghman-Estark ◽  
Reza Shoja Razavi ◽  
Hossein Edris

Scandia, yttria doped zirconia ((ZrO2)0.96(REO1.5)0.04(RE=Sc3+, Y3+)) nanoparticles were prepared by the modified sol-gel method. The microstructure of the products was characterized by X-ray diffractometry (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Raman spectroscopy. Thermal stabillity of SYSZ nanocrystals were also investigated. The SYSZ nanocrystals synthesized with EGM:Zr+4mole ratio 4:1, calcined at 700°C, have average diameter of ~20 nm.


1988 ◽  
Vol 102 (12) ◽  
pp. 1102-1106 ◽  
Author(s):  
M. Gulisano ◽  
G. Polli ◽  
G. Biondi ◽  
P. Pacini

AbstractTo date only few and often disagreeing studies about human nasopharynx are available.The present research has the purpose to give a contribution to the knowledge of nasopharyngeal epithelium using S.E.M The study was carried out on biopsies taken from 20 healthy volunteers.The surface of nasopharynx is covered by ciliated cells, microvilli provided cells and goblet cells.Areas covered with squamous epithelium and presumably transitional epithelium were observed.The possibility that ciliated become microvilli provided cells is discussed.


2015 ◽  
Vol 21 (5) ◽  
pp. 1264-1270 ◽  
Author(s):  
Manila Chieruzzi ◽  
Stefano Pagano ◽  
Carlo De Carolis ◽  
Stefano Eramo ◽  
José M. Kenny

AbstractThe inflammatory resorption of dental root apex (i.e., the process of removal of cementum and/or dentine through the activity of resorbing cells) may show different configurations and damage the apical root structure. As knowing the morphology of resorption areas of human teeth is essential for the success of endodontic treatments, we investigated the apical resorption by scanning electron microscopy, focusing on roots with granulomas. A total of 30 teeth (with penetrating carious lesions and chronic periapical lesions) were examined, the apical third of the roots were removed and analyzed to estimate periforaminal and foraminal resorption, shape and morphology of foramen resorption, centering of the periforaminal resorption area, and diameters of each apical foramen. Periforaminal resorption was present in all samples, whereas foraminal resorption was present in 92% of cases (mainly funnel shaped). Lacunae were observed in the foraminal resorption area with an average diameter of 35±14 μm. The major and minor diameters of the foramina in teeth with resorption were 443 and 313 μm, respectively (higher than in healthy teeth). This result indicates an expansion of the apical diameters caused by the pathology, which could encourage a different clinical instrumentation for these teeth.


1988 ◽  
Vol 25 (6) ◽  
pp. 509-515 ◽  
Author(s):  
E. M. Liebler ◽  
J. F. Pohlenz ◽  
N. F. Cheville

Scanning electron microscopy of lymphoid tissue in the large intestine of three germfree calves (age 3, 6, and 7 days) revealed two different units: propria nodules and lymphoglandular complexes (LGC). Propria nodules had lymphoid tissue predominantly in lamina propria and were covered by distinct follicle-associated epithelium which lacked goblet cells; nodules were surrounded by wide crypts, which were also lined by follicle-associated epithelium towards the luminal side. Lymphoglandular complexes had lymphoid follicles in the tunica submucosa; epithelial diverticulae extended through the muscularis mucosae branching into the lymphoid nodule. In centers of lymphoglandular complexes, protrusions of lymphoid tissue were covered with distinct follicle-associated epithelium. By transmission electron microscopy cells compatible with M cells in the small intestine of calves and cells with characteristics of both enteroabsorptive and M cells were found. Follicle-associated epithelium of propria nodules and lymphoglandular complexes differed only in the relative frequency of cell types.


2013 ◽  
Vol 744 ◽  
pp. 428-431
Author(s):  
Yan Long Sun ◽  
Li Min Dong ◽  
Tao Jiang ◽  
Cao Guo ◽  
Xiao Qi Zhang

An method for making AlN(aluminum nitride) nanowires by double decomposition, and the effection of additives was described. Future more, the growth mechanism of AlN nanowire synthesis with addictive were analyzed. AlN nanowire were synthesis by he AlCl3 and NaN3 in stainless steel cauldron without solvent and using Mg and Zn as additive. X-ray diffraction (XRD) and scanning electron microscopy (SEM) were used to study characters of the AlN nanowire. It is shown that the process can produce AlN nanowire with mean diameters ranging from 50 to 100 nm at 450°C. The additive has effective facilitated on the synthesis of aluminum nitride nanowires, can improve the properties of aluminum nitride nanowire, minish the average diameter of aluminum nitride nanowires.


Development ◽  
1993 ◽  
Vol 117 (1) ◽  
pp. 307-317 ◽  
Author(s):  
S.M. Purcell ◽  
R. Keller

Ceratophrys ornata, the Argentinean horned frog, has a significantly different pattern of early morphogenesis than does the most studied amphibian, Xenopus laevis. Time-lapse videomicroscopy, scanning electron microscopy, histological sections and lineage tracers have shown that, in C. ornata, some prospective notochord, somite and tailbud mesoderm cells leave the surface epithelium of the archenteron by ingression. After gastrulation, SEM reveals cells with constricted apices and a bottle shape in three zones on the archenteron roof and in a fourth zone around the blastopore. Prospective somitic tissue ingresses first from two lateral zones, followed by ingression of prospective notochord from the medial zone and tailbud mesoderm from the circumblastoporal zone. This is unlike X. laevis, in which no cells with constricted apices are present on the dorsal surface of the archenteron, nor do any cells ingress into the deep mesodermal layers from the surface layer.


2021 ◽  
pp. 096739112110271
Author(s):  
Reyhaneh Fatahian ◽  
Mohammad Mirjalili ◽  
Ramin Khajavi ◽  
Mohammad Karim Rahimi ◽  
Navid Nasirizadeh

Differences in the properties of poly(vinyl alcohol) (PVA) and poly (lactic acid) (PLA) polymers have attracted much attention today. In this research, the aim is to produce PVA/PLA nanofibers with hydrophilicity and good mechanical properties using a mutual solvent. In this regard, the ability to produce PVA/PLA nanofibers using a mutual solvent was evaluated. The effect of electrospinning parameters on the morphology of nanofibers, hydrophilicity of nanofibers produced by measuring water absorption and contact angle as well as mechanical properties of nanofibers were considered. The results obtained from scanning electron microscopy analyses of the structure of these fibers showed that PVA had the highest viscosity of 5.64 Pa.s and the highest diameter of 260 nm, which decreased the thickness of the nanofibers with increasing PLA. And pure PLA had the lowest mean diameter of 76 nm. In the consideration of the mechanical properties of the prepared nanofibers, it was found that the combination of PLA and PVA nanofibers will lead to overlap the properties of each other and the creation of desirable mechanical properties. Moreover, in the investigation of water absorption and contact angle, it was concluded that the PVA/PLA was fully absorbed in less than 200 seconds and the samples have a contact angle of less than 52°. Finally, it was found that the average diameter of the produced nanofibers was decreased by increasing the voltage and the needle tip to collector distance by considering the PVA/PLA samples with a ratio of 50:50.


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