Comparative study of four known species of the genus Acrobeles von Linstow, 1877 (Nematoda, Cephalobidae) with ‘single’ and ‘double’ cuticle from coastal dunes in Spain

Abstract The nematode genus Acrobeles is composed of two morphological groups distinguished by the presence (‘double’ cuticle) or absence (‘single’ cuticle) of the refringent inner layer of the cuticle. In the present study, four species of this genus, two with ‘single’ cuticle (Acrobeles ciliatus and Acrobeles cylindricus) and two with ‘double’ (Acrobeles aenigmaticus and Acrobeles complexus) are studied from coastal dunes in Spain. This study provides detailed morphological and morphometrical analyses for the four species, while molecular analysis, based on 18S and 28S ribosomal DNA, is provided for A. complexus. The four species are studied with scanning electron microscopy, which is obtained for the first time for A. cylindricus. These analyses revealed morphological and molecular differentiations between both groups, appearing as two related monophyletic entities. The subgenera Acrobeles and Seleborca, formerly considered as separate genera, are proposed to accommodate both groups.

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SCANNING ELECTRON MICROSCOPE: POSSIBILITIES FOR STUDYING KIDNEY DISEASES

"Medical & pharmaceutical journal "Pulse" ◽

10.26787/nydha-2686-6838-2020-22-12-176-182 ◽

2020 ◽

pp. 176-182

Author(s):

Mamaeva S.N. ◽

Vinokurov R.R. ◽

Munkhalova Ya.A. ◽

Dyakonova D.P. ◽

Platonova V.A. ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Electron Microscope ◽

Scanning Electron Microscope ◽

Red Blood Cells ◽

Blood Cells ◽

Filtration Rate ◽

Kidney Diseases ◽

First Time ◽

Scanning Electron

Currently, due to the intensive development of high-tech science-intensive medical and research devices, more and more attention is paid to the development of diagnostics of rare and difficult to diagnose diseases. It is known that among numerous nephropathies, hematuria may be the only symptom of kidney and urinary tract diseases, which complicates their diagnosis and treatment. In order to develop new approaches for the diagnosis of nephropathies, the authors have been studying the morphology of red blood cells in the blood and urine of children and adults using a scanning electron microscope for several years. The paper presents the results of studies of children with various kidney diseases, including IgA-nephropathy, and chronic glomerulonephritis. Scanning electron microscopy was used for the first time to detect nanoparticles on the surface of red blood cells, the size of which is comparable to the size of viruses, which became the basis for one of the authors ' assumptions, namely, the possible transport of certain types of viruses by red blood cells. Thus, some kidney diseases could be considered virus-associated. This paper presents for the first time the results of determining the glomerular filtration rate of both kidneys separately in the study of separate kidney function and of the study of urine smears obtained during catheterization of the ureters in patients with hydronephrosis of one of the kidneys by scanning electron microscopy. As in previous studies, nanoparticles were found on the surface of red blood cells, which leads to the conclusion about the possible viral nature of the disease of the considered patient. In addition, smear images obtained using a microscope showed a significant difference in the elements of the right and left kidneys urine, which did not contradict the data on the study of glomerular filtration rate. According to the authors, the capabilities of the scanning electron microscope can be applied in fundamental research of kidney diseases at the cellular and molecular levels, forming new ideas about their origin, as well as on the basis of which new methods of non-invasive diagnostics can be built.

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Scanning Electron Microscopy Analysis of the Anterior Capsulotomy Edge: A Comparative Study between Femtosecond Laser-Assisted Capsulotomy and Manual Capsulorhexis

Journal of Ophthalmology ◽

10.1155/2018/8620150 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Daniele Tognetto ◽

Chiara De Giacinto ◽

Alberto Armando Perrotta ◽

Tommaso Candian ◽

Alessandro Bova ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Femtosecond Laser ◽

Comparative Study ◽

Surface Irregularity ◽

Significant Difference ◽

Group 2 ◽

Cut Surface ◽

Scanning Electron ◽

Group 1

Purpose. To compare the capsule edges ultrastructure obtained by two femtosecond laser-assisted cataract surgery (FLACS) platforms and manual continuous curvilinear capsulorhexis (CCC) using scanning electron microscopy (SEM). Setting. Eye Clinic, University of Trieste, Italy. Design. Experimental comparative study. Methods. 150 anterior capsules were collected and divided into three groups as follows: Group 1 (50 capsules) obtained with manual CCC, Groups 2 and 3 (each with 50 capsules) obtained with the Catalys Laser and the LenSx Laser, respectively. All samples were imaged by means of SEM and regularity of the cut surface, and thickness of the capsule edge were evaluated and compared. Results. All femtosecond laser (FSL) capsules were perfectly circular, whereas some alteration of the circular shape was observed in the manual ones. Group 1 showed a smooth and regular capsule edge without any surface irregularity, conversely Groups 2 and 3 showed postage-stamp perforations on the capsule edge. The cut surface irregularity value in Group 2 was 1.4 ± 0.63, while it was 0.7 ± 0.49 in Group 3 (p<0.05). Group 1 had a significantly lower thickness of the capsule edge than the FSL groups (p<0.05). No statistically significant difference in the capsule edge thickness between the FSL groups was found (p=0.244). Conclusions. Despite the presence of slight cut surface irregularities, both FSL capsulotomies showed a better geometry and circularity than the manual ones. Capsulotomy specimens obtained using both FSL capsulotomies showed laser-induced alterations of the capsule edge when compared with smooth and regular edges obtained using manual CCC.

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Labellum and gynostemium micromorphology in Polystachya (Orchidaceae)

Plant Systematics and Evolution ◽

10.1007/s00606-020-01724-1 ◽

2020 ◽

Vol 307 (1) ◽

Author(s):

Mytnik Joanna ◽

Davies L. Kevin ◽

Narajczyk Magdalena ◽

Łuszczek Dorota ◽

Kubiak Joanna ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Sub Saharan Africa ◽

Capitate Trichomes ◽

Sub Saharan ◽

First Time ◽

Scanning Electron

AbstractPolystachya is a large, pantropical orchid genus of 200 species, most of which occur as epiphytes in sub-saharan Africa. The three-lobed labellum of most Polystachya species possesses a fleshy callus and various types of trichomes and papillae. In this paper, we present the results of micromorphological studies on the labellum of 20 species, representing eight of the 13 sections in the genus, using scanning electron microscopy (SEM). Our results show the scale of infrageneric diversity of trichomes and papillae relative to the sampled sections. They also demonstrate the taxonomic value of labellar micromorphology at the sectional level. The study revealed seven types of papillae and five types of trichomes (uni- and multi-cellular) in Polystachya, some of which, are described here for the first time. Clavate trichomes are the most common and are present in 60% of the species studied. Moniliform trichomes mainly occur in sect. Polystachya and are strongly characteristic of the section. Pseudopollen is formed by fragmentation of moniliform trichomes or the detachment of other trichomes as bicellular units. We provide, for the first time, evidence for the detachment of the terminal cells of capitate trichomes.

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Scanning Electron Microscopy Coupled with Energy Dispersive Spectrometric Analysis Reveals for the First Time Weddellite and Sylvite Crystals on the Surface of Involucral Bracts and Petals of two Xeranthemum L. (Compositae) Species

Microscopy and Microanalysis ◽

10.1017/s1431927617000551 ◽

2017 ◽

Vol 23 (3) ◽

pp. 679-686 ◽

Cited By ~ 2

Author(s):

Milan Gavrilović ◽

Suzana Erić ◽

Petar D. Marin ◽

Núria Garcia-Jacas ◽

Alfonso Susanna ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Glandular Trichomes ◽

Energy Dispersive ◽

Spectrometric Analysis ◽

Eds Analysis ◽

Taxonomic Studies ◽

First Time ◽

Tetragonal Bipyramid ◽

Scanning Electron

AbstractIn this work, weddellite and sylvite crystals are identified for the first time on the involucral bracts and petals of Xeranthemum annuum and Xeranthemum cylindraceum using scanning electron microscopy coupled with energy dispersive spectrometric (SEM-EDS) analysis. Well-developed crystals of weddellite (CaC2O4·2H2O) occur in the form of a tetragonal bipyramid (hhl), rarely in combination of a bipyramid and tetragonal prism (h00). Indumentum of involucral bracts of X. cylindraceum consists of nonglandular and glandular trichomes. Sylvite (KCl) crystals are observed only on the petal surface of X. cylindraceum. The crystals of sylvite occur in the form of perfect cubes (hexahedrons), but some crystals are deformed, i.e., partially elongated. Taxonomic significance of investigated microcharacters as well as the use of SEM-EDS analysis in taxonomic studies of plants are discussed.

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A Comparative Study of Trichomes in Angophora Cav. And Eucalyptus L'hérit. - A Question of Homology

Australian Journal of Botany ◽

10.1071/bt9840561 ◽

1984 ◽

Vol 32 (5) ◽

pp. 561 ◽

Cited By ~ 14

Author(s):

PY Ladiges

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Comparative Study ◽

Light Microscopy ◽

Evolutionary Trends ◽

Oil Glands ◽

Ancestral Condition ◽

Scanning Electron

The trichomes of Angophora and Eucalyptus are illustrated from scanning electron microscopy and light microscopy, and evolutionary trends are discussed. Bristle glands of Angophora and Eucalyptus subgen. Blakella and Corymbia are emergent oil glands of varying lengths. Emergent oil glands occur in all other Eucalyptus subgenera but they are most conspicuous in Blakella, Corymbia and Angophora, in which they are characterized by four cap cells each ornamented with micropapillae. Hairs in Angophora are unique, being multicellular; they are also uniseriate and scattered on the epidermis. In contrast, hairs in Eucalyptus are simple extensions, short or long, of the cells on the sides of or the cap cells of the emergent oil glands, and they are not homologous with those of Angophora. Eucalyptus setosa (subgen. Blakella) and E. brockwayi (subgen. Symphyomyrtus) are two exceptions, having unicellular hairs on the epidermis, not associated with oil glands. It is suggested that this is an ancestral condition (or secondary reversal to it).

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Capture and scanning electron microscopy of individual snow crystals

Journal of Glaciology ◽

10.3189/s0022143000003981 ◽

1994 ◽

Vol 40 (134) ◽

pp. 195-197

Author(s):

E. W. Wolff ◽

A. P. Reid

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Electron Microscope ◽

Scanning Electron Microscope ◽

Liquid Nitrogen Temperature ◽

Experimental Methods ◽

Snow Crystal ◽

Snow Crystals ◽

First Time ◽

Scanning Electron

AbstractA snow crystal has been successfully collected on to a scanning electron microscope (SEM) stub in central Greenland. It was preserved at liquid-nitrogen temperature for 5 months, prior to examination in the SEM. This is believed to be the first time a snow crystal has been observed directly in the SEM and offers some new experimental methods for understanding crystals and their chemistry.

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Ascospore sculpturing and generic concepts in the Testudinaceae (syn. Zopfiaceae)

Canadian Journal of Botany ◽

10.1139/b79-017 ◽

1979 ◽

Vol 57 (2) ◽

pp. 91-99 ◽

Cited By ~ 22

Author(s):

D. L. Hawksworth

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

New Combination ◽

The Family ◽

First Time ◽

Scanning Electron

Generic concepts in the Testudinaceae (Ascomycotina, Loculoascomycetes) are reviewed with particular emphasis on the sculpturing of the ascospores which has been examined by scanning electron microscopy (SEM) for the first time. A key to the nine genera accepted in the family is provided of which two are described as new: Ulospora for U. bilgramii comb.nov. (= Zopfia bilgramii D. Hawksw. et al.), the ascospores of which have three to six deep fissures in each cell, and Zopfiofoveola for Z. punctata comb.nov. (= Zopfia punctata D. Hawksw. & C. Booth), with distinctly and regularly foveolate ascospores. One further new combination is made: Rechingeriella boudieri comb.nov. (= Zopfia boudieri Arnaud). The positions of three additional genera referred to the family by earlier authors but which are excluded from it here are also briefly discussed.

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Comparative Study of Field Emission-Scanning Electron Microscopy and Atomic Force Microscopy to Assess Self-assembled Monolayer Coverage on Any Type of Substrate

Microscopy and Microanalysis ◽

10.1017/s1431927699990475 ◽

1999 ◽

Vol 5 (6) ◽

pp. 413-419 ◽

Cited By ~ 10

Author(s):

Bernardo R.A. Neves ◽

Michael E. Salmon ◽

Phillip E. Russell ◽

E. Barry Troughton

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Atomic Force Microscopy ◽

Comparative Study ◽

Field Emission ◽

Self Assembled Monolayer ◽

Force Microscopy ◽

Atomic Force ◽

Self Assembled ◽

Scanning Electron

Abstract: In this work, we show how field emission–scanning electron microscopy (FE-SEM) can be a useful tool for the study of self-assembled monolayer systems. We have carried out a comparative study using FE-SEM and atomic force microscopy (AFM) to assess the morphology and coverage of self-assembled monolayers (SAM) on different substrates. The results show that FE-SEM images present the same qualitative information obtained by AFM images when the SAM is deposited on a smooth substrate (e.g., mica). Further experiments with rough substrates (e.g., Al grains on glass) show that FE-SEM is capable of unambiguously identifying SAMs on any type of substrate, whereas AFM has significant difficulties in identifying SAMs on rough surfaces.

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Statoblast morphology and systematics of the freshwater bryozoan Hyalinella orbisperma (Kellicott, 1882)

Canadian Journal of Zoology ◽

10.1139/z92-211 ◽

1992 ◽

Vol 70 (8) ◽

pp. 1536-1540 ◽

Cited By ~ 5

Author(s):

Anthony Ricciardi ◽

Timothy S. Wood

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Phylogenetic Relationship ◽

Georgian Bay ◽

Close Phylogenetic Relationship ◽

First Time ◽

Scanning Electron

The freshwater bryozoan Hyalinella orbisperma (Ectoprocta: Phylactolaemata), previously known only from Michigan, is added to the list of Canadian fauna from a specimen collected at Georgian Bay, Ontario. Floatoblasts match the appearance of those described from Michigan. The sessoblast of this species is recorded and illustrated for the first time. The case for other species with sessoblasts in the genus Hyalinella is extremely weak. Scanning electron microscopy of the floatoblast and sessoblast reveals a raised reticulation with interstitial tubercles covering the capsule periblast, suggesting a close phylogenetic relationship with certain Plumatella species. It is proposed that H. orbisperma be reassigned to the genus Plumatella.

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New perspectives on Nephridiacanthus major (Acanthocephala: Oligacanthorhynchidae) collected from hedgehogs in Iran

Journal of Helminthology ◽

10.1017/s0022149x20000073 ◽

2020 ◽

Vol 94 ◽

Cited By ~ 1

Author(s):

O.M. Amin ◽

M. Sharifdini ◽

R.A. Heckmann ◽

M. Zarean

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Phylogenetic Analysis ◽

Chemical Analysis ◽

Central Asia ◽

Molecular Profile ◽

X Ray ◽

The Family ◽

First Time ◽

Scanning Electron

We describe morphological features not previously reported for this old acanthocephalan Nephridiacanthus major (Bremser, 1811 in Westrumb, 1821) Golvan, 1962 first described over 200 years ago. Our specimens were collected from long-eared hedgehog Hemiechinus auritus (Gmelin, 1770) (Erinaceidae) in Iran. We compare the morphometrics of our material with others previously reported from the same host in Iran, Russia, central Asia and Europe. Our specimens had markedly smaller proboscides, proboscis hooks and lemnisci than those reported from Russia and central Asia, but comparable measurements of other structures with specimens previously described from other collections. We document our new observations with scanning electron microscopy features not previously demonstrable by other observers and provide a chemical analysis of proboscis hooks using energy-dispersive X-ray analysis for the first time. The molecular profile of this acanthocephalan, based on 18S rDNA and cox1 genes, was generated for the first time. The phylogenetic analysis showed that N. major is placed in a clade of the family Oligacanthorhynchidae, well separated from the families Moniliformidae and Gigantorhynchidae.

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