scholarly journals Scyllarus arctus (Crustacea: Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with morphological description

2010 ◽  
Vol 91 (2) ◽  
pp. 485-492 ◽  
Author(s):  
Ferran Palero ◽  
Guillermo Guerao ◽  
Paul F. Clark ◽  
Pere Abelló
Keyword(s):  
Final Stage ◽  
Dna Analysis ◽  
Detailed Examination ◽  
Sympatric Species ◽  
Western Mediterranean ◽  
Molecular Techniques ◽  
Nucleotide Sequence Analysis ◽  
Morphological Description ◽  
Western Mediterranean Sea ◽  
Phyllosoma Larvae

Advanced stages of Scyllarus phyllosoma larvae were collected by demersal trawling during fishery research surveys in the western Mediterranean Sea in 2003–2005. Nucleotide sequence analysis of the mitochondrial 16S rDNA gene allowed the final-stage phyllosoma of Scyllarus arctus to be identified among these larvae. Its morphology is described and illustrated. This constitutes the second complete description of a Scyllaridae phyllosoma with its specific identity being validated by molecular techniques (the first was S. pygmaeus). These results also solved a long lasting taxonomic anomaly of several species assigned to the ancient genus Phyllosoma Leach, 1814. Detailed examination indicated that the final-stage phyllosoma of S. arctus shows closer affinities with the American scyllarid Scyllarus depressus or with the Australian Scyllarus sp. b (sensu Phillips et al., 1981) than to its sympatric species S. pygmaeus.

The true identities of the slipper lobsters Nisto laevis and Nisto asper (Crustacea: Decapoda: Scyllaridae) verified by DNA analysis

2009 ◽  
Vol 23 (1) ◽  
pp. 77 ◽  
Author(s):  
Ferran Palero ◽  
Guillermo Guerao ◽  
Paul F. Clark ◽  
Pere Abelló
Keyword(s):  
Dna Analysis ◽  
Historical Review ◽  
Western Mediterranean ◽  
History Museum ◽  
Larval Stages ◽  
The North ◽  
Western Mediterranean Sea ◽  
Eastern Atlantic Ocean ◽  
Reference Collection ◽  
North Western

In 1885, Sarato created Nisto as a new genus of slipper lobster and assigned two species to his taxon, namely Nisto laevis and N. asper. Bouvier later regarded these two species as post-larval stages of Scyllarus arctus (Linnaeus, 1758). This viewpoint of two post-larval stages has been accepted and retained, including being cited in the most recent scyllarid literature. The discovery of N. laevis and N. asper specimens in the reference collection of the Natural History Museum, London and the capture of three nisto-stage individuals, one in the north-western Mediterranean Sea and two in the Gulf of Cádiz (eastern Atlantic Ocean) have facilitated a historical review into the post-larval stages of S. arctus, as well as new detailed morphological descriptions. From the present study, it has been possible to describe and illustrate the nisto stages of S. arctus and S. pygmaeus (Bate, 1888) and to resolve the true identities of N. laevis and N. asper by DNA analysis. Accordingly, N. laevis has been identified as the nisto stage of S. pygmaeus and N. asper as that of S. arctus.

scholarly journals Genetic differentiation among Parastichopus regalis populations from Western Mediterranean Sea: potential effects of its fishery and current connectivity.

2015 ◽  
Vol 16 (3) ◽  
pp. 489 ◽  
Author(s):  
C. MAGGI ◽  
M. GONZÁLEZ-WANGÜEMERT
Keyword(s):  
Genetic Diversity ◽  
Genetic Differentiation ◽  
Haplotype Diversity ◽  
Western Mediterranean ◽  
Mediterranean Coast ◽  
Morphological Data ◽  
Coi Gene ◽  
Fixation Index ◽  
Morphological Description ◽  
Western Mediterranean Sea

Parastichopus regalis (Cuvier, 1817) is the most expensive seafood product on the catalonian market (NE Spain), with prices around 130 €/Kg (fresh weight). Despite its ecological and economic importance, biological and genetic information on this sea cucumber species is scarce. We provided the first insight on the genetic structure of P. regalis using sequences of cytochrome oxidase I (COI) and 16S genes, as well as a morphological description of its populations. Individuals were collected in six locations along the Spanish Mediterranean coast, including an area under fishery pressure (Catalonia). We found high haplotype diversity and low nucleotide diversity for both genes, with higher levels of genetic diversity observed on COI gene. Population pairwise fixation index (FST), AMOVA and correspondence analysis (CA) based on COI, revealed significant genetic differentiation among some locations. However, further analysis using nuclear markers (e.g. microsatellites) would be necessary to corroborate these results. Moreover, the genetic and morphological data may indicate fishery effects on the Catalonian population with decrease of the size and weight average and lower genetic diversity compared to locations without fishery pressure. For an appropriate management of this species, we suggest: 1) an accurate assessment of the stocks status along the Spanish coasts; 2) the study of the reproductive cycle of this target species and the establishment of a closed fishery season according to it; 3) the founding of protected areas (i.e. not take zones) to conserve healthy populations and favour the recruitment on the nearby areas.

Abnormal Processing of the Glycoprotein IIb Transcript due to a Nonsense Mutation in Exon 17 Associated with Glanzmann’s Thrombasthenia

1995 ◽  
Vol 73 (05) ◽  
pp. 756-762 ◽  
Author(s):  
Yoshiaki Tomiyama ◽  
Hirokazu Kashiwagi ◽  
Satoru Kosugi ◽  
Masamichi Shiraga ◽  
Yoshio Kanayama ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Molecular Genetic ◽  
Genetic Defect ◽  
Nucleotide Sequence Analysis ◽  
Type I ◽  
Normal Amount ◽  
Immunoblot Assay ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Pcr Products

SummaryWe analyzed the molecular genetic defect responsible for type I Glanzmann’s thrombasthenia in a Japanese patient. In an immunoblot assay using polyclonal anti-GPIIb-IIIa antibodies, some GPIIIa (15% of normal amount) could be detected in the patient’s platelets, whereas GPIIb could not (<2% of normal amount). Nucleotide sequence analysis of platelet GPIIb mRNA-derived polymerase chain reaction (PCR) products revealed that patient’s GPIIb cDNA had a 75-bp deletion in the 3’ boundary of exon 17 resulting in an in-frame deletion of 25 amino acids. DNA analysis and family study revealed that the patient was a compound heterozygote of two GPIIb gene defects. One allele derived from her father was not expressed in platelets, and the other allele derived from her mother had a 9644C → T mutation which was located at the position -3 of the splice donor junction of exon 17 and resulted in a termination codon (TGA). Moreover, quantitative analysis demonstrated that the amount of the abnormal GPIIb transcript in the patient’s platelets was markedly reduced. Thus, the C → T mutation resulting in the abnormal splicing of GPIIb transcript and the reduction in its amount is responsible for Glanzmann’s thrombasthenia.

scholarly journals Tracking the global reduction of marine traffic during the COVID-19 pandemic

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
David March ◽  
Kristian Metcalfe ◽  
Joaquin Tintoré ◽  
Brendan J. Godley
Keyword(s):  
Large Scale ◽  
Human Mobility ◽  
Western Mediterranean ◽  
Western Mediterranean Sea ◽  
Marine Traffic ◽  
Human Movements ◽  
Exclusive Economic Zones ◽  
Global Impacts ◽  
Economic Zones

AbstractThe COVID-19 pandemic has resulted in unparalleled global impacts on human mobility. In the ocean, ship-based activities are thought to have been impacted due to severe restrictions on human movements and changes in consumption. Here, we quantify and map global change in marine traffic during the first half of 2020. There were decreases in 70.2% of Exclusive Economic Zones but changes varied spatially and temporally in alignment with confinement measures. Global declines peaked in April, with a reduction in traffic occupancy of 1.4% and decreases found across 54.8% of the sampling units. Passenger vessels presented more marked and longer lasting decreases. A regional assessment in the Western Mediterranean Sea gave further insights regarding the pace of recovery and long-term changes. Our approach provides guidance for large-scale monitoring of the progress and potential effects of COVID-19 on vessel traffic that may subsequently influence the blue economy and ocean health.

scholarly journals Parasites of the head of Scomber colias (Osteichthyes: Scombridae) from the western Mediterranean Sea

2014 ◽  
Vol 59 (1) ◽  
Author(s):  
Salvatore Mele ◽  
Maria Pennino ◽  
Maria Piras ◽  
José Bellido ◽  
Giovanni Garippa ◽  
...  
Keyword(s):  
Mediterranean Sea ◽  
Eastern Mediterranean ◽  
Western Mediterranean ◽  
Parasite Fauna ◽  
Point Of View ◽  
Published Data ◽  
Multivariate Techniques ◽  
Western Mediterranean Sea ◽  
Scomber Colias ◽  
Non Parametric

AbstractThe metazoan parasite assemblage of the head of 30 specimens of the Atlantic chub mackerel (Scomber colias) from the western Mediterranean Sea was analysed. Eight species of parasites were found, four mazocraeid monogeneans: Grubea cochlear (prevalence = 10%), Kuhnia scombercolias (59%), K. scombri (52%), Pseudokuhnia minor (86%); three didymozoid trematodes: Nematobothrium cf. faciale (21%), N. filiforme (41%), N. scombri (7%); and one laerneopodid copepod: Clavelissa scombri (7%). Results were compared with previously published data from 14 localities of the eastern Mediterranean Sea and the Atlantic Ocean, using non-parametric univariate and multivariate analyses, and the whole parasite fauna of S. colias was compared with that of the congeners (S. australasicus, S. japonicus and S. scombrus). Parasites showed to reflect the biogeographical and phylogenetic history of host. From a methodological point of view, the use of both non-parametric univariate and multivariate techniques proved to be effective tools to detect dissimilarities between parasite assemblages.

scholarly journals Dynamical Downscaling of ERA5 Data on the North-Western Mediterranean Sea: From Atmosphere to High-Resolution Coastal Wave Climate

2021 ◽  
Vol 9 (2) ◽  
pp. 208
Author(s):  
Valentina Vannucchi ◽  
Stefano Taddei ◽  
Valerio Capecchi ◽  
Michele Bendoni ◽  
Carlo Brandini
Keyword(s):  
Mediterranean Sea ◽  
Wind Wave ◽  
Dynamical Downscaling ◽  
Wave Climate ◽  
Western Mediterranean ◽  
Limited Area ◽  
Computational Domain ◽  
Wave Hindcast ◽  
The North ◽  
Western Mediterranean Sea

A 29-year wind/wave hindcast is produced over the Mediterranean Sea for the period 1990–2018. The dataset is obtained by downscaling the ERA5 global atmospheric reanalyses, which provide the initial and boundary conditions for a numerical chain based on limited-area weather and wave models: the BOLAM, MOLOCH and WaveWatch III (WW3) models. In the WW3 computational domain, an unstructured mesh is used. The variable resolutions reach up to 500 m along the coasts of the Ligurian and Tyrrhenian seas (Italy), the main objects of the study. The wind/wave hindcast is validated using observations from coastal weather stations and buoys. The wind validation provides velocity correlations between 0.45 and 0.76, while significant wave height correlations are much higher—between 0.89 and 0.96. The results are also compared to the original low-resolution ERA5 dataset, based on assimilated models. The comparison shows that the downscaling improves the hindcast reliability, particularly in the coastal regions, and especially with regard to wind and wave directions.

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