IgG response of rats and humans to the released products of schistosomula of Schistosoma mansoni

Parasitology ◽  
1985 ◽  
Vol 90 (3) ◽  
pp. 509-518 ◽  
Author(s):  
Claudie Verwaerde ◽  
C. Auriault ◽  
Martine Damonneville ◽  
J.-M. Grzych ◽  
R. Pierce ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Surface Proteins ◽  
Brown Norway ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Molecular Weights ◽  
Wide Range ◽  
Human Sera ◽  
Norway Rats

The participation of products released from Schistosoma mansoni schistosomula (SRP-A) in the IgG antibody response of infected Brown-Norway rats and infected humans has been studied using immunoprecipitation with various antigenic preparations and in in vitro cytotoxicity assays. A large number of SRP-A molecules with a wide range of molecular weights was recognized by infected rat and human sera. Anti-SRP-A antibodies appeared in rat sera from day 28 after infection. In infected humans, a variable pattern of SRP-A recognition was observed between individuals. IgG antibodies obtained by immunization of rats with SRP-A without addition of adjuvants reacted with 3 major schistosomula surface proteins with molecular weights of 38, 32 and 21 kDa. These latter molecules were also revealed strongly by infected rat sera. Moreover, these antibodies were able to kill schistosomula in vitro in the presence of complement or eosinophils.

scholarly journals Bioaffinity-based Surface Immobilization of Antibodies to Capture Endothelial Colony-forming Cells

2021 ◽  
Author(s):  
Mariève D Boulanger ◽  
Mohamed A Elkhodiry ◽  
Omar Bashth ◽  
Gaétan Laroche ◽  
Corinne A Hoesli
Keyword(s):  
Surface Modification ◽  
Surface Proteins ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G ◽  
Cell Capture ◽  
Igg Antibodies ◽  
Immobilized Antibodies ◽  
Endothelial Colony Forming Cells ◽  
Covalent Grafting

Maximizing the re-endothelialization of vascular implants such as prostheses or stents has the potential to significantly improve their long-term performance. Endothelial progenitor cell capture stents with surface-immobilized antibodies show significantly improved endothelialization in the clinic. However, most current antibody-based stent surface modification strategies rely on antibody adsorption or direct conjugation via amino or carboxyl groups which leads to poor control over antibody surface concentration and/or molecular orientation, and ultimately bioavailability for cell capture. Here, we assess the utility of a bioaffinity-based surface modification strategy consisting of a surface-conjugated cysteine-tagged protein G molecules that immobilize Immunoglobulin G (IgG) antibodies via the Fc domain to capture circulating endothelial colony-forming cells (ECFCs). The cysteine-tagged protein G was grafted onto aminated substrates at different concentrations as detected by an enzyme-linked immunosorbent assay and fluorescence imaging. Different IgG antibodies were successfully immobilized on the protein G-modified surfaces and higher antibody surface concentrations were achieved compared to passive adsorption methods. Surfaces with immobilized antibodies targeting endothelial surface proteins, such as CD144, significantly enhanced the capture of circulating ECFCs in vitro compared to surfaces with non-endothelial specific antibodies such as anti-CD14. This work presents a potential avenue for enhancing the clinical performance of vascular implants by using covalent grafting of protein G to immobilize IgG antibodies more effectively.

scholarly journals Evolution of IgG antibody response against Toxoplasma gondii tissue cyst in acute and chronic human infections

1998 ◽  
Vol 40 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Margarita VILLAVEDRA ◽  
Hernán CAROL ◽  
Alberto NIETO
Keyword(s):  
Toxoplasma Gondii ◽  
Acute Infection ◽  
Chronic Phase ◽  
Tissue Cyst ◽  
Specific Response ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Wide Range ◽  
Toxoplasmic Infection ◽  
Human Infections

The recognition profile of the tissue cysts antigens by IgG antibodies was studied during acute and chronic human toxoplasmic infection. Thus the IgG response against Toxoplasma gondii was investigated by immunoblotting in two patients accidentally infected with the RH strain as well as in group of naturally infected patients at acute and chronic phase. There was an overall coincidence of molecular mass among antigens of tachyzoites and tissue cysts recognized by these sera, however, they appear not to be the same molecules. The response against tissue cysts starts early during acute infection, and the reactivity of antibodies is strong against a wide range of antigens. Six bands (between 82 and 151 kDa) were exclusively recognized by chronic phase sera but only the 132 kDa band was positive in more than 50% of the sera analysed. A mixture of these antigens could be used to discriminate between the two infection phases. The most important antigens recognized by the acute and the chronic phase sera were 4 clusters in the ranges 20-24 kDa, 34-39 kDa, 58-80 kDa and 105-130 kDa as well as two additional antigens of 18 and 29 kDa. Both accidentally infected patients and some of the naturally infected patients showed a weak specific response against tissue cyst antigens.

scholarly journals Evaluation of Red and Green Colored Bell peppers for the Production of Polyclonal IgM and IgG Antibodies in Murine Spleen Cells

2021 ◽  
Vol 24 (1) ◽  
pp. 45-53
Author(s):  
Md Moklesur Rahman Sarker
Keyword(s):  
B Cells ◽  
Antibody Production ◽  
Bacterial Infections ◽  
Pharmaceutical Journal ◽  
Bell Pepper ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Green Pepper ◽  
Bell Peppers

Immunostimulants are greatly required for the upregulation of immunity to fight against viral and bacterial infections and cancers. Bell peppers (Capsicum annuum L.), eaten as vegetables, are rich sources of vitamin C and E, provitamin A, β-carotene, and numerous phenolic compounds. Antimicrobial, antioxidant, anti-mutagenic and anti-inflammatory properties of Bell peppers were reported. Our research group for the first time reported the immunomodulatory activities of Bell peppers. In this study, we evaluated the antibody production abilities of two different colored Bell peppers (red and green) in the culture of antibody producing splenic B cells of mice. Antibodies and the number of viable cells were determined by an ELISA and MTT assays, respectively. Red Bell pepper Extract (RBPE) at the doses of (0.375, 0.75, 1.5, and 2.25 mg/mL) significantly augmented the production of polyclonal IgM and IgG antibodies in-vitro. The highest amount of IgM antibody production was observed by the dose of 1.5 mg/kg which was 3 times higher than that of the untreated cells. Similarly, RBPE also enhanced the production of IgG antibody in the culture of murine splenic B cells. On the contrary, cultural treatment of murine splenic B cells with Green Bell pepper Extract (GBPE) could not stimulate the B cells, and hence, failed to produce neither IgM nor IgG antibody. Thus the current findings suggest that consumption of Red Bell Pepper extract or its vegetables, not green pepper, may be beneficial to strengthen humoral immune responses. Bangladesh Pharmaceutical Journal 24(1): 45-53, 2021

scholarly journals Inhibitory Effects of Diclofenac on Steroid Glucuronidation in Vivo Do not Affect Hair-Based Doping Tests for Stanozolol

2017 ◽  
Author(s):  
Gergely Zachár ◽  
Nawed I. K. Deshmukh ◽  
Andrea Petróczi ◽  
Andrea D. Székely ◽  
Iltaf Shah ◽  
...  
Keyword(s):  
Steroid Metabolism ◽  
Brown Norway ◽  
Inhibitory Effects ◽  
Spot Urine ◽  
Rat Study ◽  
Weekly Assessment ◽  
Urine And Serum ◽  
Norway Rats

In vitro studies show that diclofenac inhibits enzymatic steroid glucuronidation. This study was designed to investigate the influence of diclofenac on the excretion of stanozolol and 3'-hydroxystanozolol via analyses in hair, blood and urine in vivo in a rat study. Brown Norway rats were administered with stanozolol (weeks 1-3) and diclofenac (weeks 1-6). Weekly assessment of steroid levels in hair was complemented with spot urine and serum tests. Levels of both stanozolol and 3'-hydroxystanozolol steadily increased in hair during stanozolol treatment and decreased post-treatment, but remained readily detectable for 6 weeks. In contrast, compared to control rats, diclofenac significantly reduced urinary excretion of 3’-hydroxystanozolol which was undetectable in most samples. This is the first report of diclofenac altering steroid metabolism in vivo, detrimentally affecting detection in urine, but not in hair which holds considerable advantages over urinalysis for anti-doping tests.

scholarly journals A survey of coxsackie A16 virus antibodies in human sera

1984 ◽  
Vol 93 (2) ◽  
pp. 205-212 ◽  
Author(s):  
G. E. D. Urquhart
Keyword(s):  
Spontaneous Abortion ◽  
Adult Female ◽  
Foot And Mouth Disease ◽  
Antibody Detection ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Detection Rates ◽  
Common Cause ◽  
Human Sera ◽  
Antibody Tests

SummaryA comparison of neutralizing and immunofluorescent (IF) IgG antibody tests in 18 sera from 10 cases of hand, foot and mouth disease (HFMD) showed a variety of responses but all sera taken more than one week after infection had both neutralizing and IF IgG antibodies.A survey of IF IgG antibody in 80 paediatric and 80 adult non-HFMD case sera gave antibody detection rates of 47·5% and 11·3% respectively. This difference could be attributed to a decline in IF IgG antibody with time after infection. Three point nine per cent of 2G sera from possible adult carditis cases had IF antibody suggesting that coxsackie A16 virus was not a common cause of adult carditis. Forty-eight point three per cent of 29 sera from cases of spontaneous abortion had detectable IF antibody, a rate similar to the paediatric sera and significantly greater than that in adult male (7·9%) and other adult female (13%) sera tested. This interesting observation requires further study.

Antibody to carbohydrate and polypeptide epitopes on the surface of schistosomula of Schistosoma mansoni in Egyptian patients with acute and chronic schistosomiasis

Parasitology ◽  
1989 ◽  
Vol 98 (3) ◽  
pp. 417-424 ◽  
Author(s):  
P. Omer Ali ◽  
M. Mansour ◽  
J. N. Woody ◽  
S. R. Smithers ◽  
A. J. G. Simpson
Keyword(s):  
Schistosoma Mansoni ◽  
Chronic Infection ◽  
Acute Infection ◽  
Surface Antigens ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Human Antibodies ◽  
Serum Pool ◽  
Surface Recognition ◽  
Egyptian Patients

SUMMARY125I-Schistosoma mansoni schistosomulum surface antigens were immunoprecipitated with human antibodies from individual Egyptian patients diagnosed as being either acutely or chronically infected with S. mansoni. Both sets of patients were found to have IgG antibodies in their sera capable of immunoprecipitating the major Mr > 200, 38 and 32K antigens. However, the immunoprecipitation of the Mr 200K antigen was found to constitute a significantly greater proportion of the total precipitate achieved with acute sera than with chronic sera. The Mr 38 and 32K antigens were more variably precipitated by the acute sera than the chronic sera but the proportion of the total precipitation that these two antigens constituted was not found to be significantly different between the two sets of sera. Immunoprecipitation with pooled antibodies absorbed with egg and adult worm homogenates which had been treated to remove either carbohydrate or polypeptide epitopes demonstrated that the Mr > 200K antigen was the principal target of egg-cross-reactive anti-carbohydrate antibody amongst the antigens detected. The Mr 38 and 32K antigens were found to be precipitated by antibodies to protease-sensitive and periodate-insensitive polypeptide epitopes. These results are consistent with egg-cross-reactive anti-carbohydrate IgG antibody making a greater contribution to schistosomulum surface recognition in acute infection than in chronic infection. Indeed the presence of a higher level of egg-cross-reactive and anti-carbohydrate antibody directed against schistosomulum surface epitopes in an acute serum pool than in a chronic serum pool was confirmed by measurement of antibody binding to whole schistosomula.

scholarly journals Attenuating Effect of Peruvian Cocoa Populations on the Acute Asthmatic Response in Brown Norway Rats

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2301
Author(s):  
Marta Périz ◽  
Francisco J. Pérez-Cano ◽  
Trinitat Cambras ◽  
Àngels Franch ◽  
Ivan Best ◽  
...  
Keyword(s):  
Mast Cell ◽  
Allergic Asthma ◽  
Immunoglobulin E ◽  
In Vivo Study ◽  
Brown Norway ◽  
Mast Cell Protease ◽  
Norway Rats ◽  
Anaphylactic Response

Cocoa contains bioactive components, which vary according to genetic and environmental factors. The present study aimed to ascertain the anti-allergic properties of native Peruvian cocoa populations (“Blanco de Piura” or BPC, “Amazonas Peru” or APC, “Criollo de Montaña” or CMC, “Chuncho” or CCC, and an ordinary cocoa or OC). To do so, after an initial in vitro approach, an in vivo study focused on the induction of an anaphylactic response associated with allergic asthma in Brown Norway rats was carried out. Based on their polyphenol content, antioxidant activity and in vitro effects, the APC and CMC were selected to be included in the in vivo study. Cocoa diets were tested in a model of allergic asthma in which anaphylactic response was assessed by changes in body temperature, motor activity and body weight. The concentration of specific immunoglobulin E (IgE), mast cell protease and leukotrienes was also quantified in serum and/or bronchoalveolar lavage fluid. CMC and OC populations exhibited a protective effect on the allergic asthma rat model as evidenced by means of a partial protection against anaphylactic response and, above all, in the synthesis of IgE and the release of mast cell protease.

Inhibitory action of Wnt target gene osteopontin on mitochondrial cytochrome c release determines renal ischemic resistance

2010 ◽  
Vol 299 (1) ◽  
pp. F234-F242 ◽  
Author(s):  
Jose Luis Viñas ◽  
Anna Sola ◽  
Michaela Jung ◽  
Chrysoula Mastora ◽  
Eugenia Vinuesa ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Wnt Pathway ◽  
Brown Norway ◽  
Mitochondrial Cytochrome ◽  
Cytochrome C Release ◽  
Norway Rats ◽  
Mitochondrial Cytochrome C

Certain determinants of ischemic resistance in the Brown Norway rat strain have been proposed, but no studies to date have focused on the role of the Wnt pathway in the ischemic resistance mechanism. We performed a comparative genomic study in Brown Norway vs. Sprague-Dawley rats. Selective manipulations of the Wnt pathway in vivo and in vitro allowed us to study whether the action of the Wnt pathway on apoptosis through the regulation of osteopontin was critical to the maintenance of inherent ischemic resistance mechanisms. The results revealed a major gene upregulation of the Wnt family in Brown Norway rats after renal ischemia-reperfusion. Manipulation of the Wnt signaling cascade by selective antibodies increased mitochondrial cytochrome c release and caspase 3 activity. The antiapoptotic role of Wnt was mediated by osteopontin, a direct Wnt target gene. Osteopontin was reduced by Wnt antibody administration in vivo, and osteopontin gene silencing in vitro significantly increased mitochondrial cytochrome c release. The overexpression of Wnt pathway genes detected in Brown Norway rats is critical in the maintenance of their inherent ischemic resistance. Activation of the Wnt signaling cascade reduces mitochondrial cytochrome c release and caspase 3 activity through the action of osteopontin.

scholarly journals Partially Hydrolysed Whey Has Superior Allergy Preventive Capacity Compared to Intact Whey Regardless of Amoxicillin Administration in Brown Norway Rats

2021 ◽  
Vol 12 ◽  
Author(s):  
Katrine Bækby Graversen ◽  
Jeppe Madura Larsen ◽  
Signe Schultz Pedersen ◽  
Laila Vestergaard Sørensen ◽  
Heidi Frahm Christoffersen ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Physicochemical Properties ◽  
Infant Formula ◽  
Human Infant ◽  
Brown Norway ◽  
Allergy Prevention ◽  
Microbiota Composition ◽  
Norway Rats ◽  
Gut Microbiota Composition

BackgroundIt remains largely unknown how physicochemical properties of hydrolysed infant formulas influence their allergy preventive capacity, and results from clinical and animal studies comparing the preventive capacity of hydrolysed infant formula with conventional infant formula are inconclusive. Thus, the use of hydrolysed infant formula for allergy prevention in atopy-prone infants is highly debated. Furthermore, knowledge on how gut microbiota influences allergy prevention remains scarce.ObjectiveTo gain knowledge on (1) how physicochemical properties of hydrolysed whey products influence the allergy preventive capacity, (2) whether host microbiota disturbance influences allergy prevention, and (3) to what extent hydrolysed whey products influence gut microbiota composition.MethodsThe preventive capacity of four different ad libitum administered whey products was investigated in Brown Norway rats with either a conventional or an amoxicillin-disturbed gut microbiota. The preventive capacity of products was evaluated as the capacity to reduce whey-specific sensitisation and allergic reactions to intact whey after intraperitoneal post-immunisations with intact whey. Additionally, the direct effect of the whey products on the growth of gut bacteria derived from healthy human infant donors was evaluated by in vitro incubation.ResultsTwo partially hydrolysed whey products with different physicochemical characteristics were found to be superior in preventing whey-specific sensitisation compared to intact and extensively hydrolysed whey products. Daily oral amoxicillin administration, initiated one week prior to intervention with whey products, disturbed the gut microbiota but did not impair the prevention of whey-specific sensitisation. The in vitro incubation of infant faecal samples with whey products indicated that partially hydrolysed whey products might confer a selective advantage to enterococci.ConclusionsOur results support the use of partially hydrolysed whey products for prevention of cow’s milk allergy in atopy-predisposed infants regardless of their microbiota status. However, possible direct effects of partially hydrolysed whey products on gut microbiota composition warrants further investigation.

scholarly journals Schistosoma mansoni soluble egg antigens enhance HCV replication in mammalian cells

2010 ◽  
Vol 4 (04) ◽  
pp. 226-234 ◽  
Author(s):  
Mahmoud Mohamed Bahgat ◽  
Mohamed Abd-Elhafez El-Far ◽  
Ahmed Atef Mesalam ◽  
Amany Abd-Elghany Ismaeil ◽  
Ahmed Atef Ibrahim ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Blood Cells ◽  
Hepg2 Cells ◽  
Mammalian Cells ◽  
Culture Media ◽  
Rt Pcr ◽  
Western Blots ◽  
Molecular Weights ◽  
Viral Shedding ◽  
Human Sera

Background: This work demonstrates successful propagation of HCV in HepG2 and human blood cells as well as viral shedding into their culture media. The influence of Schistosoma mansoni crude soluble egg antigens (SEA) on the rate of viral propagation in both mammalian cells was also monitored. Methodology: HepG2 cells were inoculated with HCV viremic human sera and some wells were exposed to HCV infection in presence of SEA. Cells were harvested for RT-PCR and Western blotting analysis. HepG2 media was collected for HCV ELISA. Blood samples from HCV-infected humans were cultured in the presence and absence of SEA. Media were collected at different time points post culturing and subjected to HCV ELISA. Results: The ELISA concentration of HCV antigens were generally higher in media of infected HepG2 cells compared to media of control cells at all time intervals post infection. Western blots showed reactivity to immunogenic peptides of different molecular weights in lysate of infected HepG2 cells that were not evidenced in uninfected cells. In presence of SEA, RT-PCR results revealed earlier detection of viral RNA in infected HepG2 cells compared to in absence of such bilharzial antigen. Also, ELISA results revealed higher levels of detected HCV antigens in media of both infected HepG2 and blood cells cocultured with S. mansoni SEA compared to that of cultured infected cells in absence of the parasite antigens. Conclusion: HepG2 cells as well as whole blood cultures maintain HCV replication. Furthermore, SEA has the potential to enhance HCV propagation.

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