A new microsporidian parasite, Heterosporis saurida n. sp. (Microsporidia) infecting the lizardfish, Saurida undosquamis from the Arabian Gulf, Saudi Arabia: ultrastructure and phylogeny

SUMMARYA new microsporidian that infects the lizardfish Saurida undosquamis (Richardson, 1848) that are caught in the Arabian Gulf in Saudi Arabia is described here. This parasite invades the skeletal muscle of the abdominal cavity forming white, cyst-like structures containing numerous spores. The prevalence of the infection was 32·1% (135/420). The spores were oval to pyriform in shape and measured approximately 3·3 μm×2·0 μm. The developing spores were found within parasitophorous vacuoles. In mature spores, the polar filament was arranged into 5 coils in a row. Molecular analysis of the rRNA genes, including the ITS region, and phylogenetic analyses using maximum parsimony, maximum likelihood, and Bayesian inference were performed. The ultrastructural characteristics and phylogenetic analyses support the recognition of a new species, herein named Heterosporis saurida n. sp.

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Lactarius guangdongensis sp. nov. (Russulaceae, Russulales), a species of Lactarius sect. Deliciosi growing with a vulnerable five-needle pine, Pinus kwangtungensis

Phytotaxa ◽

10.11646/phytotaxa.393.3.4 ◽

2019 ◽

Vol 393 (3) ◽

pp. 278

Author(s):

YING HAN ◽

CUN-ZHU LIANG ◽

PEI-GUI LIU ◽

JORINDE NUYTINCK ◽

XIANG-HUA WANG

Keyword(s):

New Species ◽

South China ◽

Ex Situ Conservation ◽

Phylogenetic Analyses ◽

Its Region ◽

Morphological Characters ◽

Ex Situ ◽

Dehydrogenase Gene ◽

A New Species

Lactarius sect. Deliciosi is a group of ectomycorrhizal mushrooms showing high host specificity with trees of Pinaceae and more rarely Fagales. A new species of this section, L. guangdongensis, is described from South China. This new species has the smallest basidiocarps (pileus 1–3 cm) in the section. The pure orange basidiocarps with faint greenish discoloration are similar to those of Abies-associated L. abieticola and L. laeticolor and Pinus-associated L. vividus. The subdistant lamellae and absence of pleuromacrocystidia can be used as valid characters for this species. It grows with Chinese national protected plant Pinus kwangtungensis and might be one of the very few species that form symbiosis with five-needle pines. Phylogenetic analyses of the ITS region and glyceraldehyde-3-phosphate dehydrogenase gene (gpd) show that L. guangdongensis clearly diversified from its relatives and look-alikes and probably represents an early diverging lineage in the section. Molecular and morphological characters provided in this study will be useful for in situ and ex situ conservation of P. kwangtungensis in the future.

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Cytological and molecular description of Hamiltosporidium tvaerminnensis gen. et sp. nov., a microsporidian parasite of Daphnia magna, and establishment of Hamiltosporidium magnivora comb. nov.

Parasitology ◽

10.1017/s0031182010001393 ◽

2010 ◽

Vol 138 (4) ◽

pp. 447-462 ◽

Cited By ~ 27

Author(s):

KAREN LUISA HAAG ◽

J. I. RONNY LARSSON ◽

DOMINIK REFARDT ◽

DIETER EBERT

Keyword(s):

Daphnia Magna ◽

Sequence Data ◽

Polar Filament ◽

Molecular Data ◽

Phylogenetic Position ◽

Microsporidian Parasite ◽

Isolated Nuclei ◽

Host Interactions ◽

Ultrastructural Characteristics ◽

Molecular Description

SUMMARYWe describe the new microsporidium Hamiltosporidium tvaerminnensis gen. et sp. nov. with an emphasis on its ultrastructural characteristics and phylogenetic position as inferred from the sequence data of SSU rDNA, alpha- and beta-tubulin. This parasite was previously identified as Octosporea bayeri Jírovec, 1936 and has become a model system to study the ecology, epidemiology, evolution and genomics of microsporidia - host interactions. Here, we present evidence that shows its differences from O. bayeri. Hamiltosporidium tvaerminnensis exclusively infects the adipose tissue, the ovaries and the hypodermis of Daphnia magna and is found only in host populations located in coastal rock pool populations in Finland and Sweden. Merogonial stages of H. tvaerminnensis have isolated nuclei; merozoites are formed by binary fission or by the cleaving of a plasmodium with a small number of nuclei. A sporogonial plasmodium with isolated nuclei yields 8 sporoblasts. Elongated spores are generated by the most finger-like plasmodia. The mature spores are polymorphic in shape and size. Most spores are pyriform (4·9–5·6×2·2–2·3 μm) and have their polar filament arranged in 12–13 coils. A second, elongated spore type (6·8–12·0×1·6–2·1 μm) is rod-shaped with blunt ends and measures 6·8–12·0×1·6–2·1 μm. The envelope of the sporophorous vesicle is thin and fragile, formed at the beginning of the sporogony. Cytological and molecular comparisons with Flabelliforma magnivora, a parasite infecting the same tissues in the same host species, reveal that these two species are very closely related, yet distinct. Moreover, both cytological and molecular data indicate that these species are quite distant from F. montana, the type species of the genus Flabelliforma. We therefore propose that F. magnivora also be placed in Hamiltosporidium gen. nov.

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Low genetic diversity among Francisella-like endosymbionts within different genotypes of Hyalomma dromedarii ticks infesting camels in Saudi Arabia

Veterinary World ◽

10.14202/vetworld.2020.1462-1472 ◽

2020 ◽

Vol 13 (7) ◽

pp. 1462-1472

Author(s):

Haitham Elbir ◽

Faisal Almathen ◽

Ayman Elnahas

Keyword(s):

Genetic Diversity ◽

Saudi Arabia ◽

Population Structure ◽

16S Rrna ◽

Sequence Similarity ◽

Phylogenetic Analyses ◽

16S Rrna Genes ◽

Rrna Genes ◽

Hyalomma Dromedarii ◽

Low Genetic Diversity

Background and Aim: Hyalomma dromedarii ticks are vectors of disease agents and hosts of Francisella-like endosymbionts (FLEs). Knowledge about intraspecific genetic variation among H. dromedarii and its Francisella species is limited. The aims of this study were to investigate whether certain H. dromedarii genotypes are specialized in carrying specific Francisella species genotypes and scrutinize the population structure of H. dromedarii ticks in Saudi Arabia. Materials and Methods: We collected 151 H. dromedarii ticks from 33 camels from 13 locations in Saudi Arabia. The second internal transcribed spacer (ITS2), cytochrome c oxidase subunit-1(COI), and 16S rRNA genes were used for single-and multi-locus sequence typing and phylogenetic analyses. H. dromedarii-borne Francisella was screened using the tul4 gene and 16S rRNA Francisella-specific primers followed by amplicon Sanger sequencing. Results: Single-locus typing of ticks using ITS2, 16S rRNA, and COI genes yielded 1, 10, and 31 sequence types (ST), respectively, with pairwise sequence similarity of 100% for ITS2, 99.18-99.86% for COI, and 99.50-99.75% for 16S rRNA. COI sequence analysis indicated a lack of strict geographical structuration, as ST15 was found in both Saudi Arabia and Kenya. In contrast, multilocus sequence typing resolved 148 H. dromedarii ticks into 39 genotypes of ticks and three genotypes of FLEs. The ST2-FLE genotype was carried by the tick genotype ST35, while the ST1-FLE genotype and 41.89% of the ST3-FLE genotype were carried by the tick genotype ST32. Accordingly, there appeared to be no specialization of certain tick genotypes to harbor-specific FLE genotypes. Conclusion: For the 1st time, we have provided an overview of the population structure of H. dromedarii ticks and FLE strains. We found a low level of genetic diversity among FLEs and non-specialized circulation of FLEs among H. dromedarii ticks.

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Review of the moray eels (Anguilliformes: Muraenidae) of the Red Sea, with description of a new species

Zootaxa ◽

10.11646/zootaxa.4704.1.1 ◽

2019 ◽

Vol 4704 (1) ◽

pp. 1-87 ◽

Cited By ~ 2

Author(s):

DAVID G. SMITH ◽

SERGEY V. BOGORODSKY ◽

AHMAD O. MAL ◽

TILMAN J. ALPERMANN

Keyword(s):

Saudi Arabia ◽

Red Sea ◽

New Record ◽

Phylogenetic Analyses ◽

Common Species ◽

Valid Species ◽

Nominal Species ◽

Dorsal Fin ◽

A New Species ◽

Preanal Length

Thirty-eight species of moray eels (Muraenidae) from the Red Sea are reported in an updated review. A species account is provided for each species, along with a full synonymy of all nominal species described from the Red Sea. One species is new to science, G. pharaonis, and two species are new for the Red Sea, Gymnothorax phasmatodes (Smith) and Scuticaria tigrina (Lesson). Gymnothorax pharaonis n. sp. is a common species that has long been misidentified as G. undulatus (Lacepède), although it more closely resembles G. margaritophorus Bleeker, to which it is closely related phylogenetically. It is characterized by the following combination of characters: total vertebrae 123–128; body slender, depth at anus 17–28 in TL; maxillary teeth biserial, dentary and vomerine teeth in a single row; color brown with irregular dendritic pale markings, not interconnected or chain-like, with oblique, conspicuous, parallel streaks posteriorly in dorsal fin. The new record of the distinctive whitish G. phasmatodes is based on an underwater photograph taken at Jeddah, Saudi Arabia. The new record of Scuticaria tigrina is based on a specimen collected from Jeddah, Saudi Arabia, and underwater photographs taken from the northern part of the Red Sea. Based on an integrative taxonomical approach by a combination of morphological and phylogenetic analyses and re-examination of holotype specimens, Gymnothorax cinerascens (Rüppell) is resurrected as a valid species, and it is shown that previous records of G. hepaticus (Rüppell), with which it has previously been synonymized, should refer to G. cinerascens. The true G. hepaticus is redescribed based on examination of the holotype and additional specimens collected during the course of this study. The two species are distinguished by the color of the jaws, the position of the median intermaxillary teeth, and a slight difference in the preanal length. They are also clearly distinct genetically. The Red Sea record of Gymnothorax atolli (Pietschmann) is based on an error, possibly a misidentification of G. griseus (Lacepède). A key to the species of Red Sea moray eels and a phylogenetic tree of presently known lineages of Indo-Pacific moray eels are provided including recently collected Red Sea specimens.

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Zygotorulaspora dagestanica sp. nov., a novel ascomycetous yeast species associated with the Georgian honeysuckle (Lonicera iberica M. Bieb.)

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijsem.0.004785 ◽

2021 ◽

Vol 71 (4) ◽

Author(s):

Aleksey V. Kachalkin ◽

Dinakhanum A. Abdullabekova ◽

Elena S. Magomedova ◽

Andrey M. Yurkov

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Its Region ◽

Rrna Gene ◽

Ascomycetous Yeast ◽

Wild Fruit ◽

Large Subunit Rrna ◽

A New Species ◽

Basal Position

During an investigation of the yeast communities associated with wild fruit shrubs in Dagestan (Caucasus, Russia), four fermenting ascospore-producing yeast strains were isolated from leaves of the Georgian honeysuckle (Lonicera iberica M. Bieb.) and from soil underneath this plant. Phylogenetic analyses based on concatenated sequences of the ITS region and D1/D2 domains of the large subunit rRNA gene and concatenated sequences of the ribosomal DNA cystron, RPB2 and TEF1 genes showed that the isolated strains represented a new species of the genus Zygotorulaspora. The new species was placed in the basal position to other species of the clade and close to Zygotorulaspora mrakii. Based on the results of phylogenetic analyses and the phenotypic characteristics of the four studied strains, a novel species is described, for which the name Zygotorulaspora dagestanica sp. nov. is proposed. The holotype is KBP Y-4591T, three metabolically inactive cryopreserved isotype cultures are DSM 100088, VKM Y-3060 and VKPM Y-4318. The MycoBank number is MB 838285.

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Rotylenchus castilloi n. sp. (Nematoda: Hoplolaimidae), a new species with long stylet from northern Iran

Zootaxa ◽

10.11646/zootaxa.3931.1.6 ◽

2015 ◽

Vol 3931 (1) ◽

pp. 88 ◽

Cited By ~ 6

Author(s):

ATEFEH TALEZARI ◽

EBRAHIM POURJAM ◽

AHMAD KHEIRI ◽

GRACIA LIÉBANAS ◽

FARZAD ALIRAMAJI ◽

...

Keyword(s):

New Species ◽

Body Length ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Rrna Genes ◽

Bisexual Species ◽

Northern Iran ◽

Stylet Length ◽

A New Species ◽

Specific Sequences

Rotylenchus castilloi n. sp., a new bisexual species is described and illustrated based on morphological, morphometric and molecular data. The new species is characterised by having a hemispherical, continuous lip region with an irregular corncob-like appearance under SEM, very long stylet (62–68 µm), vulva located at 49.7–62.2% of body length from anterior end, with a protruding double epiptygma, a rounded to convex-conoid (rarely bi-lobed) tail with 8–12 annuli and specific sequences of D2-D3 segments of 28S and ITS1-rRNA genes. Differences between the new species and four other species of the genus (R. mesorobustus, R. cazorlaensis, R. magnus and R. jaeni) are discussed. Morphologically, the new species can be separated from these species mostly by its body length, lip region characters, stylet length and location of phasmid. Phylogenetic analyses using 721 bp partial sequences of D2-D3 expansion segments of the 28S and 590 bp ITS1-rRNA genes revealed the new species forming a clade with two isolates of R. eximius and two isolates of R. unisexus, two morphologically unrelated species.

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Nodosilinea chupicuarensis sp. nov. (Leptolyngbyaceae, Synechococcales) a subaerial cyanobacterium isolated from a stone monument in central Mexico

Phytotaxa ◽

10.11646/phytotaxa.334.2.6 ◽

2018 ◽

Vol 334 (2) ◽

pp. 167 ◽

Cited By ~ 13

Author(s):

JUAN VÁZQUEZ-MARTÍNEZ ◽

JUAN MANUEL GUTIERREZ-VILLAGOMEZ ◽

CITLALI FONSECA-GARCÍA ◽

ENRIQUE RAMÍREZ-CHÁVEZ ◽

MA. LOURDES MONDRAGÓN-SÁNCHEZ ◽

...

Keyword(s):

Phylogenetic Analyses ◽

Its Region ◽

Molecular Data ◽

Central Mexico ◽

Rrna Gene ◽

North Central ◽

Stone Monuments ◽

Morphological And Molecular Data ◽

A New Species ◽

The 16S Rrna Gene

Microorganisms of subaerial biofilms, which develop on the surface of stone monuments, have been widely studied due to their impact on Cultural Heritage deterioration. Nevertheless, these extremophile microorganisms are poorly characterised. In this study, a cyanobacterial strain isolated from an archaeological zone in north-central Mexico was characterised through a polyphasic study based on ecological, morphological, and molecular data. Phylogenetic analyses were done using sequences of the 16S rRNA gene and the 16S-23S ITS region. Based on the polyphasic data, a new species corresponding to the genus Nodosilinea is described. This alkaliphilic species has optimum growth at pH of 9. Its fatty acid profile indicates that C18:1, 9E and C16:0 are its most abundant fatty acids. Extracellular polymeric substance production was verified on this strain. Only a few species of the genus Nodosilinea have been documented and described, and this is the first report of their presence in central Mexico. Hence the name Nodosilinea chupicuarensis sp. nov. is proposed.

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A new species of the genus Pseudocrangonyx (Crustacea: Amphipoda: Pseudocrangonyctidae) from Simbok Cave, Korea

Zootaxa ◽

10.11646/zootaxa.4731.3.2 ◽

2020 ◽

Vol 4731 (3) ◽

pp. 321-334 ◽

Cited By ~ 4

Author(s):

CHI-WOO LEE ◽

KO TOMIKAWA ◽

TAKAFUMI NAKANO ◽

GI-SIK MIN

Keyword(s):

Phylogenetic Analyses ◽

Histone H3 ◽

16S Rrna Genes ◽

Rrna Genes ◽

28S Rrna ◽

Mitochondrial Cytochrome ◽

Molecular Phylogenetic ◽

Subterranean Species ◽

A New Species ◽

Honshu Island

A new subterranean species of pseudocrangonyctid amphipod, Pseudocrangonyx joolaei, is described from the groundwater of a cave in South Korea. Pseudocrangonyx joolaei sp. nov. can be distinguished from its congeners by the number of sternal gills as well as a combination of the antennal sinus, the accessory flagellum of antenna 1, and the terminal article of uropod 3. Molecular phylogenetic analyses based on nuclear 28S rRNA and histone H3, and mitochondrial cytochrome c oxidase subunit I and 16S rRNA genes revealed that P. joolaei sp. nov. formed a clade with P. akatsukai Tomikawa & Nakano, 2018 that inhabits limestone caves in the western Honshu island, Japan.

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A new species of Tricholosporum (Agaricales, Tricholomataceae) from Liaoning Province of China

Phytotaxa ◽

10.11646/phytotaxa.374.1.5 ◽

2018 ◽

Vol 374 (1) ◽

pp. 63 ◽

Cited By ~ 1

Author(s):

JIZE XU ◽

CHUNLAN ZHANG ◽

TAIGA KASUYA ◽

ODESHNEE MOODLEY ◽

BAO LIU ◽

...

Keyword(s):

New Species ◽

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Its Region ◽

Molecular Data ◽

Liaoning Province ◽

Morphological And Molecular Data ◽

A New Species

Tricholosporum haitangshanum sp. nov. is described as a new species from China, based on morphological and molecular data. It is morphologically characterized by a yellowish ochre pileus, cruciate basidiospores and ampullaceous cystidia. Phylogenetic analyses based on the nuc rDNA internal transcribed spacer (ITS) region supports that it belongs to Tricholosporum. Comparing it with phenotypically similar and molecular phylogenetically allied taxa concluded that it is a new species in Tricholosporum.

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Puccinia marrubii (Pucciniaceae), a new rust species on Marrubium globosum subsp. globosum from Niğde and Malatya in Turkey

Phytotaxa ◽

10.11646/phytotaxa.272.4.5 ◽

2016 ◽

Vol 272 (4) ◽

pp. 277 ◽

Cited By ~ 1

Author(s):

ŞANLI KABAKTEPE ◽

BİROL MUTLU ◽

ŞÜKRÜ KARAKUŞ ◽

ILGAZ AKATA

Keyword(s):

New Species ◽

Bayesian Methods ◽

Related Species ◽

Phylogenetic Relationships ◽

Phylogenetic Analyses ◽

Rust Fungus ◽

Morphological Characteristics ◽

Its Region ◽

First Time ◽

A New Species

A new species of rust fungus, Puccinia marrubii sp. nov. is described from Turkey. The new species is the first Puccinia species to be identified on genus Marrubium (Lamiaceae). In order to determine the phylogenetic relationships of the new species, ITS region was amplified by PCR, sequenced, and analyzed. In addition to the ITS region of seven Puccinia species, P. annularis, P. jasmini, P. malvacearum, P. nigrescens, P. phlomidis, P. salviae, and P. serpylli were sequenced for the first time. Phylogenetic analyses performed by using distance, Bayesian methods confirmed that the new rust was closely related to P. serpylli, P. malvacearum, P. salviae, and P. annularis. The diagnostic morphological characteristics of the new species were discussed with the phylogenetically related species.

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