Genetic diversity and phylogeography of the elusive, but epidemiologically important Echinococcus granulosus sensu stricto genotype G3

Parasitology ◽  
2018 ◽  
Vol 145 (12) ◽  
pp. 1613-1622 ◽  
Author(s):  
Liina Kinkar ◽  
Teivi Laurimäe ◽  
Ibrahim Balkaya ◽  
Adriano Casulli ◽  
Houria Zait ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Echinococcus Granulosus ◽  
Phylogenetic Network ◽  
Sensu Stricto ◽  
Phylogeographic Analysis ◽  
Definition Of ◽  
Human Infections ◽  
Insight Into ◽  
Genotype G3 ◽  
Complete Mtdna

AbstractCystic echinococcosis (CE) is a severe parasitic disease caused by the species complex Echinococcus granulosus sensu lato. Human infections are most commonly associated with E. granulosus sensu stricto (s.s.), comprising genotypes G1 and G3. The objective of the current study was to provide first insight into the genetic diversity and phylogeography of genotype G3. Despite the epidemiological importance of the genotype, it has remained poorly explored due to the ambiguity in the definition of the genotype. However, it was recently demonstrated that long sequences of mitochondrial DNA (mtDNA) provide a reliable method to discriminate G1 and G3 from each other. Therefore, we sequenced near-complete mtDNA of 39 G3 samples, covering most of the known distribution range and host spectra of the genotype. The phylogenetic network revealed high genetic variation within E. granulosus s.s. G3 and while G3 is significantly less prevalent worldwide than G1, the genetic diversity of both of the genotypes is equally high. We also present the results of the Bayesian phylogeographic analysis, which yielded several well-supported diffusion routes of genotype G3 originating from Turkey and Iran, suggesting the Middle East as the origin of the genotype.

scholarly journals Human Cystic Echinococcosis in Lebanon: A Retrospective Study and Molecular Epidemiology

2021 ◽  
Author(s):  
Gaelle Joanny ◽  
Maria Grazia Cappai ◽  
Francesca Nonnis ◽  
Claudia Tamponi ◽  
Giorgia Dessì ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Cystic Echinococcosis ◽  
Epidemiological Data ◽  
Sensu Stricto ◽  
Control Measures ◽  
Mediterranean Countries ◽  
High Gene ◽  
Human Infections ◽  
And Control ◽  
Human Cystic Echinococcosis

Abstract Purpose Human cystic echinococcosis (CE) is a zoonotic parasitic disease that constitutes a public health challenge and a socio-economic burden in endemic areas worldwide. No specific surveillance system of CE infections in humans exists in Lebanon. The incidence and trends over time have not been documented. The current study aimed to assess the demographic and epidemiologic features of human CE surgical cases over a 14-year period in the five main regions of Lebanon. Methods From 2005 to 2018, a total of 894 surgically confirmed cases of hydatidosis were recorded from five anatomy and pathology laboratories. Results The mean annual surgical incidence was 1.23/100,000 inhabitants. Over the span of these years, the incidence increased from 0.53 to 1.94 cases/100,000 inhabitants in 2005 and 2018, respectively. CE is present in Lebanon with an uneven distribution from one region to the other with higher prevalence in Bekaa (29.0%), a rural area where sheep raising is widespread. Human CE cases were more common in females (60.1%) than in males (39.9%) and a high burden of infection was reported for the age group of 30–39 years. Besides, 66.7% of the cases expressed only liver complications whereas, 20.5% showed predilection towards lungs. The 7.8% of cases presented cysts in other organs, and 1.3% showed multiple localizations. Additionally, predominant involvement of Echinococcus granulosus sensu stricto was recorded in human infections. Comparison of Echinococcus granulosus s.s. populations from different Mediterranean countries also revealed high gene flow among this region and sharing of alleles. Conclusion The current study is a step forward to fill the gap of knowledge for the hydatidosis in Lebanon where the lack of epidemiological data and control measures have resulted in higher incidence of human CE. Graphic Abstract

Investigating the genetic diversity of Echinococcus granulosus sensu stricto with new microsatellites

2018 ◽  
Vol 117 (9) ◽  
pp. 2743-2755 ◽  
Author(s):  
Gérald Umhang ◽  
Frédéric Grenouillet ◽  
Vanessa Bastid ◽  
Selim M’Rad ◽  
Benoît Valot ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Echinococcus Granulosus ◽  
Sensu Stricto ◽  
Echinococcus Granulosus Sensu Stricto

First report of Echinococcus granulosus sensu stricto (G1) in Nigeria, West Africa

2019 ◽  
Vol 94 ◽  
Author(s):  
J.A. Ohiolei ◽  
H.-B. Yan ◽  
L. Li ◽  
C. Isaac ◽  
B.-Q. Fu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
West Africa ◽  
Host Range ◽  
Echinococcus Granulosus ◽  
Cystic Echinococcosis ◽  
Sensu Stricto ◽  
Human Infection ◽  
Zoonotic Potential ◽  
The West ◽  
Echinococcus Granulosus Sensu Stricto

Abstract Echinococcus granulosus sensu stricto is regarded to have the highest zoonotic potential of all Echinococcus taxa. Globally, human infection due to this species constitutes over 88.44% of the total cystic echinococcosis (CE) burden. Here, we report a CE infection in a Nigerian camel caused by E. granulosus G1 genotype. To the best of our knowledge, this report is the first encounter of the G1 genotype in the West Africa sub-region where the G6 genotype is reportedly prevalent, suggesting that the epidemiology of this highly zoonotic group could have a wider host range and distribution in the sub-region, and emphasizes the need for further investigation into the genetic diversity of Echinococcus spp. in Nigeria and across the sub-region.

scholarly journals Genetic diversity of Echinococcus granulosus sensu stricto infecting humans in western Algeria

2021 ◽  
Author(s):  
Daouia Moussa ◽  
Kheira Senouci ◽  
Nori Midoun ◽  
Mohamed Lacheheb ◽  
Benali Tabeti ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Echinococcus Granulosus ◽  
Sensu Stricto ◽  
Echinococcus Granulosus Sensu Stricto ◽  
Western Algeria

scholarly journals Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Echinococcus granulosus Sensu Stricto Complex

2020 ◽  
Author(s):  
Abolghasem SIYADATPANAH ◽  
Ahmad DARYANI ◽  
Shahabeddin SARVI ◽  
Adel SPOTIN ◽  
Mehdi SHARIF ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Echinococcus Granulosus ◽  
Haplotype Diversity ◽  
Taxonomic Status ◽  
Sensu Stricto ◽  
Ffpe Tissue ◽  
Cox1 Gene ◽  
Northern Iran ◽  
Tissue Samples ◽  
Echinococcus Granulosus Sensu Stricto

Background: Human Echinococcosisis a cyclo-zoonotic infection caused by tapeworms of the Echinococcus granulosus sensu stricto complex. The detection of mitochondrial genome data of genus Echinococcus can reflect the taxonomic status, genetic diversity, and population structure genetics. Methods: Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 (cox1) gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity. Results: Molecular analysis showed that 50(96.1%) and 2 (3.84%) isolates were identified as G1 andG3 E. granulosus genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46%; G1 genotype) and 2 (3.84%; G3 genotype) new haplotypes were unequivocally identified. Conclusion: G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of Echinococcus parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran.

Investigation of the relationship between CE cyst characteristics and genetic diversity of Echinococcus granulosus sensu lato in humans from Turkey

Parasitology ◽  
2020 ◽  
Vol 147 (14) ◽  
pp. 1712-1717
Author(s):  
Serra Örsten ◽  
Türkmen Çiftçi ◽  
Aynur Azizova ◽  
Gökhan Yüce ◽  
Aycan Uysal ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Echinococcus Granulosus ◽  
Rural Areas ◽  
Sensu Stricto ◽  
Genetic Identification ◽  
Cyst Volume ◽  
Gene Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
The Relationship ◽  
Echinococcus Granulosus Sensu Lato

AbstractCystic echinococcosis (CE) is one of the most common zoonotic diseases worldwide, particularly in rural areas. This study aimed at the identification of the genotype/species belonging to Echinococcus granulosus sensu lato (s.l.) specimens in retrieved percutaneously from the human host and to investigate their relationship with cyst characteristics. The genetic identification of cyst material was performed by mt-CO1 gene polymerase chain reaction, and confirmed via sequencing. A total of 110 CE cysts were identified as E. granulosus s.l. In detail, 104 belonged to E. granulosus sensu stricto (G1 and G3) and six isolates were in the E. canadensis cluster (G6/7). All clusters were tested for the relationship between demographics, cyst features and genetic diversity. The relationship between genetic variation and certain clinical characteristics such as cyst volume and location were statistically significant for G6/7 cluster. Further studies are required with a larger sample set to investigate the relationship between the genetic variability of E. granulosus s.l. and cyst features.

First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia

2016 ◽  
Vol 223 ◽  
pp. 57-62 ◽  
Author(s):  
Zoran Debeljak ◽  
Belgees Boufana ◽  
Maria Interisano ◽  
Dejan Vidanovic ◽  
Zoran Kulisic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Echinococcus Granulosus ◽  
Sensu Stricto ◽  
Echinococcus Granulosus Sensu Stricto

Frequency and genetic diversity of Echinococcus granulosus sensu stricto in sheep and cattle from the steppe region of Djelfa, Algeria

2018 ◽  
Vol 118 (1) ◽  
pp. 89-96 ◽  
Author(s):  
Abd Elkarim Laatamna ◽  
Dennis Ebi ◽  
Khadidja Brahimi ◽  
Khadidja Bediaf ◽  
Marion Wassermann ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Echinococcus Granulosus ◽  
Sensu Stricto ◽  
Echinococcus Granulosus Sensu Stricto ◽  
Steppe Region
Sign in / Sign up

Export Citation Format

Share Document