Linear Arrays of Helical Polyribosomes in Cells Exposed to Antitumor Drug Vincristine Sulfate

1969 ◽  
Vol 27 ◽  
pp. 358-359
Author(s):  
Awtar Krishan
Keyword(s):  
Close Association ◽  
Annulate Lamellae ◽  
Large Array ◽  
Vincristine Sulfate ◽  
Linear Arrays ◽  
Golgi Membranes ◽  
Related Drug ◽  
Drug Leads ◽  
Lethal Doses ◽  
Vinblastine Sulfate

Earle's L-929 fibroblasts treated with mitosis-arresting but sub-lethal doses of vinblastine sulfate (VLB) show hypertrophy of the granular endoplasmic reticulum and annulate lamellae. Exposure of the cells to heavier doses of vincristine sulfate (VCR), a VLB-related drug, leads to the accumulation of large amounts of helical polyribosomes, Golgi membranes and crystals in the cytoplasm. In many of these cells a large number of helical polyribosomes are arranged in prominent linear rows, some of which may be up to 5 micrometers in length. Figure 1 shows a large array of helical polyribosomes near a crystalline mass (CRS) in an Earle's L-929 fibroblast exposed to VCR (5ϒ/ml.) for 3 hours At a higher magnification, as seen in figure 2, the helical polyribosomes are seen arranged in parallel rows. In favorably cut sections, a prominent backbone like "stalk" of finely granular material, measuring approximately 300Å in width is seen in close association with the linear rows of helical polyribosomes.

scholarly journals THE FINE STRUCTURE AND IMMUNOLOGICAL LABELING OF THE ACHROMATIC MITOTIC APPARATUS AFTER DISRUPTION OF CELL MEMBRANES

1973 ◽  
Vol 59 (3) ◽  
pp. 643-660 ◽  
Author(s):  
Samuel Dales ◽  
Konrad C. Hsu ◽  
Ariaki Nagayama
Keyword(s):  
Nuclear Pore Complex ◽  
Membrane Disruption ◽  
Nuclear Pore ◽  
Annulate Lamellae ◽  
Immunoperoxidase Method ◽  
Mitotic Apparatus ◽  
Uniform Size ◽  
Nonionic Detergent ◽  
Antibody Conjugates ◽  
Vinblastine Sulfate

After treatment of HeLa and L cells with vinblastine sulfate the material of microtubules (tubulin) was reorganized into (a) large paracrystals (PC) of tightly packed tubules; (b) smaller aggregates of tubules with greater diameter whose walls are constituted from well defined, helically arranged morphological subunits; and (c) microtubules associated with helices of polyribosomes of uniform size. All of these structures survived disruption of cellular membranes by means of a nonionic detergent. Following a thorough stripping of membranes there remained a subcellular fraction sedimenting at 1,500 g for 15 min, in which were contained nuclei, centrioles, and the above mentioned microtubular elements, maintained as a complex of organelles by an interconnecting network of 80 Å microfibrils. As a result of membrane disruption it was possible to localize precisely in the electron microscope the binding of ferritin antibody conjugates. Specific labeling at the surface of PC and microtubule aggregates could be demonstrated. This result was substantiated by means of the immunoperoxidase method of labeling the PC. A concentrated deposit of ferritin was also found in the vicinity of centrioles and related structures, the annuli of the nuclear pore complex and the annulate lamellae. However, the specificity of the label on these organelles remains questionable because ferritin, albeit in lower concentration, was also present on them in control preparations reacted with preimmune sera.

scholarly journals Sensitive and Rapid Spectrophotometric Methods for Determination of Anticancer Drugs

2002 ◽  
Vol 85 (5) ◽  
pp. 1021-1024 ◽  
Author(s):  
Padmarajaiah Nagaraja ◽  
Ramanathapura A Vasantha ◽  
Hemmige S Yathirajan
Keyword(s):  
Anticancer Drugs ◽  
Pharmaceutical Formulations ◽  
Subsequent Reaction ◽  
Vincristine Sulfate ◽  
Spectrophotometric Methods ◽  
Hydrochloric Acid Medium ◽  
Beer's Law ◽  
Beer’S Law ◽  
Vinblastine Sulfate

Abstract Sensitive, rapid, and simple spectrophotometric methods were developed for determination of the anticancer drugs vinblastine sulfate (VBS) and vincristine sulfate (VCS), which belong to the class of vinca alkaloids. The first method is based on the reaction of VBS and VCS with diazotized dapsone, forming yellow azo products with absorption maxima at 430 nm. The colored species obey Beer's law in the concentration range of 0.5–24 μg/mL for VBS and 0.5–12 μg/mL for VCS. The second method describes the reaction of VBS and VCS with iron(III) and subsequent reaction with ferricyanide in hydrochloric acid medium to yield blue products with absorption maxima at 750 nm. The Beer's law range for this method is 0.1–4 μg/mL for VBS and 0.5–10 μg/mL for VCS. With both methods, colored species were stable for 1 h. The methods are simple and reproducible and are applied for determination of VBS and VCS in pharmaceutical formulations. Commonly encountered pharmaceuticals added as excipients do not interfere in the analysis and the results obtained in the analysis of dosage forms agree well with the labeled contents.

scholarly journals Pre-M Phase-promoting Factor Associates with Annulate Lamellae inXenopus Oocytes and Egg Extracts

2003 ◽  
Vol 14 (3) ◽  
pp. 1125-1137 ◽  
Author(s):  
Clare Beckhelling ◽  
Patrick Chang ◽  
Sandra Chevalier ◽  
Chris Ford ◽  
Evelyn Houliston
Keyword(s):  
High Speed ◽  
Fluorescent Protein ◽  
Soluble Fraction ◽  
Annulate Lamellae ◽  
Golgi Membranes ◽  
M Phase ◽  
Egg Extracts ◽  
Approaches To Study ◽  
Green Fluorescent

We have used complementary biochemical and in vivo approaches to study the compartmentalization of M phase-promoting factor (MPF) in prophase Xenopus eggs and oocytes. We first examined the distribution of MPF (Cdc2/CyclinB2) and membranous organelles in high-speed extracts of Xenopus eggs made during mitotic prophase. These extracts were found to lack mitochondria, Golgi membranes, and most endoplasmic reticulum (ER) but to contain the bulk of the pre-MPF pool. This pre-MPF could be pelleted by further centrifugation along with components necessary to activate it. On activation, Cdc2/CyclinB2 moved into the soluble fraction. Electron microscopy and Western blot analysis showed that the pre-MPF pellet contained a specific ER subdomain comprising “annulate lamellae” (AL): stacked ER membranes highly enriched in nuclear pores. Colocalization of pre-MPF with AL was demonstrated by anti-CyclinB2 immunofluorescence in prophase oocytes, in which AL are positioned close to the vegetal surface. Green fluorescent protein-CyclinB2 expressed in oocytes also localized at AL. These data suggest that inactive MPF associates with nuclear envelope components just before activation. This association may explain why nuclei and centrosomes stimulate MPF activation and provide a mechanism for targeting of MPF to some of its key substrates.

Stimulated Virus Production in Vinblastine and Cytochalasin-Induced Multinucleate Cells

1970 ◽  
Vol 28 ◽  
pp. 186-187
Author(s):  
Krishan Awtar
Keyword(s):  
Cell Division ◽  
Normal Cell ◽  
Virus Production ◽  
Multinucleate Cells ◽  
Daughter Cells ◽  
Cell Divisions ◽  
Nuclear Membranes ◽  
Division 2 ◽  
Vinblastine Sulfate

Exposure of cells to low sublethal but mitosis-arresting doses of vinblastine sulfate (Velban) results in the initial arrest of cells in mitosis followed by their subsequent return to an “interphase“-like stage. A large number of these cells reform their nuclear membranes and form large multimicronucleated cells, some containing as many as 25 or more micronuclei (1). Formation of large multinucleate cells is also caused by cytochalasin, by causing the fusion of daughter cells at the end of an otherwise .normal cell division (2). By the repetition of this process through subsequent cell divisions, large cells with 6 or more nuclei are formed.

Immunocytochemical Localization of Amyloid Protein AA in the “Dense Fibrillar Inclusions” of the Reticuloendothelical Cells

1977 ◽  
Vol 35 ◽  
pp. 438-439
Author(s):  
T. Shirahama ◽  
M. Skinner ◽  
A.S. Cohen
Keyword(s):  
Amyloid Fibril ◽  
Close Association ◽  
Gel Filtration ◽  
Fibril Formation ◽  
Amyloid Protein ◽  
Electron Microscopic ◽  
Amyloid Deposits ◽  
Amyloid Fibril Formation ◽  
Electron Microscopic Technique ◽  
Lysosomal System

A1thought the mechanisms of amyloidogenesis have not been entirely clarified, proteolysis of the parent proteins may be one of the important steps in the amyloid fibril formation. Recently, we reported that "dense fibrillar inclusions" (DFI), which had the characteristics of lysosomes and contained organized fibrillar profiles as well, were observed in the reticuloendothelial cells in close association with the foci of new amyloid deposits. We considered the findings as evidence for the involvement of lysosomal system in amyloid fibril formation (l). In the present study, we attempted to determine the identity of the contents of the DFI by the use of antisera against the amyloid protein (AA) and an immuno-electron microscopic technique.Amyloidosis was induced in CBA/J mice by daily injections of casein (l). AA was isolated from amyloid-laden spleens by gel filtration and antibody to it was produced in rabbits (2). For immunocytochemistry, the unlabeled antibody enzyme method (3) was employed.

Membranous alterations in the cytoplasm and nucleus in a primitive neuroectodermal tumor of the brain

1978 ◽  
Vol 36 (2) ◽  
pp. 628-629
Author(s):  
C. N. Sun ◽  
C. Araoz ◽  
H. J. White
Keyword(s):  
Nuclear Envelope ◽  
Tumor Cells ◽  
Osmium Tetroxide ◽  
Primitive Neuroectodermal Tumor ◽  
Uranyl Acetate ◽  
Neuroectodermal Tumor ◽  
Annulate Lamellae ◽  
Lead Citrate ◽  
Thin Sections ◽  
The Brain

The ultrastructure of a cerebral primitive neuroectodermal tumor has been reported previously. In the present case, we will present some unusual previously unreported membranous structures and alterations in the cytoplasm and nucleus of the tumor cells.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4% glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1% buffered osmium tetroxide for one hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate.In the cytoplasm of the tumor cells, we found paired cisternae (Fig. 1) and annulate lamellae (Fig. 2) noting that the annulate lamellae were sometimes associated with the outer nuclear envelope (Fig. 3). These membranous structures have been reported in other tumor cells. In our case, mitochondrial to nuclear envelope fusions were often noted (Fig. 4). Although this phenomenon was reported in an oncocytoma, their frequency in the present study is quite striking.

The ultrastructure of acinar cells in the external orbital gland of young and old male rats

1978 ◽  
Vol 36 (2) ◽  
pp. 276-277
Author(s):  
R. Carriere
Keyword(s):  
Young Adulthood ◽  
Acinar Cells ◽  
Cell Number ◽  
Male Rats ◽  
Polyploid Cell ◽  
Secretory Cells ◽  
Age Changes ◽  
Albino Rat ◽  
Golgi Membranes ◽  
Diploid Cells

The external orbital gland of the albino rat exhibits both sexual dimorphism and histological age changes. In males, many cells attain a remarkable degree of polyploidy and an increase of polyploid cell number constitutes the major age change until young adulthood. The acini of young adults have a small lumen and are composed of tall serous cells. Subsequently, many acini acquire a larger lumen with an irregular outline while numerous vacuoles accumulate throughout the secretory cells. At the same time, vesicular acini with a large lumen surrounded by pale-staining low cuboidal diploid cells begin to appear and their number increases throughout old age. The fine structure of external orbital glands from both sexes has been explored and in considering acinar cells from males, emphasis was given to the form of the Golgi membranes and to nuclear infoldings of cytoplasmic constituents.

Annulate lamellae in the Sertoli cells of guinea pig testis after unilateral torsion of the spermatic cord

1984 ◽  
Vol 42 ◽  
pp. 196-197
Author(s):  
J. Chakraborty ◽  
A. P. Sinha Hikim ◽  
J. S. Jhunjhunwala
Keyword(s):  
Sertoli Cells ◽  
Guinea Pigs ◽  
Spermatic Cord ◽  
Present Report ◽  
Cell Types ◽  
Annulate Lamellae ◽  
Spermatogenic Cells ◽  
Electron Microscopic ◽  
Structural Details ◽  
First Time

Although the presence of annulate lamellae was noted in many cell types, including the rat spermatogenic cells, this structure was never reported in the Sertoli cells of any rodent species. The present report is based on a part of our project on the effect of torsion of the spermatic cord to the contralateral testis. This paper describes for the first time, the fine structural details of the annulate lamellae in the Sertoli cells of damaged testis from guinea pigs.One side of the spermatic cord of each of six Hartly strain adult guinea pigs was surgically twisted (540°) under pentobarbital anesthesia (1). Four months after induction of torsion, animals were sacrificed, testes were excised and processed for the light and electron microscopic investigations. In the damaged testis, the majority of seminiferous tubule contained a layer of Sertoli cells with occasional spermatogonia (Fig. 1). Nuclei of these Sertoli cells were highly pleomorphic and contained small chromatinic clumps adjacent to the inner aspect of the nuclear envelope (Fig. 2).

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